Additional file 4: Figure S3. Interference of LncRNA-TBP inhibits fatty acid oxidation in skeletal muscle. (A-F) Relative LncRNA-TBP expression (n = 4) (A), relative mtDNA content (n = 4) (B), relative fatty acid β-oxidation rate (n = 4) (C), relative FFA and TG content (n = 4) (D), relative mRNA (n = 6) (E) and protein (n = 3) (F) expression levels of fatty acid oxidation or synthesis related-genes in gastrocnemius with LncRNA-TBP interference in...

Additional file 4: Figure S3. Interference of LncRNA-TBP inhibits fatty acid oxidation in skeletal muscle. (A-F) Relative LncRNA-TBP expression (n = 4) (A), relative mtDNA content (n = 4) (B), relative fatty acid β-oxidation rate (n = 4) (C), relative FFA and TG content (n = 4) (D), relative mRNA (n = 6) (E) and protein (n = 3) (F) expression levels of fatty acid oxidation or synthesis related-genes in gastrocnemius with LncRNA-TBP interference in...

Additional file 5: Figure S4. Interference of LncRNA-TBP activates fast-twitch muscle phenotype and reduces muscle hypertrophy. (A-N) relative enzymes activity of LDH and SDH (n = 4) (A), Relative glycogen content (n = 5) (B), relative mRNA expression levels of glycogenolytic and glycolytic genes (n = 6) (C), relative mRNA expression levels of several fast-/slow-twitch myofiber genes (n = 6) (D), immunohistochemistry analysis of MYH1A/MYH7B (n = 3) (E), MYH1A/MYH7B protein content (n = 8)...

Additional file 9: Figure S8. The expression and location analysis of TBP. (A) Relative TBP expression during the proliferation and differentiation of CPM isolated from XH chicken (n = 4). (B) Subcellular location of TBP protein annotated by UniProt Knowledgebase ( https://www.uniprot.org/ ). In panels (A), results are presented as mean ± SEM.

Additional file 2: Figure S1. Conservative analysis of LncRNA-TBP performed by using the NCBI’s BLAST. A total of eighteen species, including Anas platyrhynchos, Anser cygnoides, Apteryx mantelli mantelli, Aquila chrysaetos, Bos taurus, Coturnix japonica, Gallus gallus, Geospiza fortis, Homo sapiens, Meleagris gallopavo, Melopsittacus undulatus, Mus musculus, Numida meleagris, Ovis aries, Pan troglodytes, Rattus norvegicus, Sus scrofa and Zebra finch were used for Nucleotide BLAST. Top 4 most conservative results were listed above.

Additional file 3: Fig. S3. The overall methylation profile of COL12A1 gene in iCCA and adjacent nontumor tissue samples from TCGA (A) and GSE156299 (B) dataset, respectively. TCGA, The Cancer Genome Atlas; iCCA, intrahepatic cholangiocarcinoma.

Additional file 1. Figure S1. RNA-seq results and characterization of MenSCs. A RNA-seq analysis of BNIP3 and BNIP3L mRNA expression in HepG2 in response to coculture with MenSCs for 72 h. C means control group, M means MenSCs coculture group (n=3). B The mRNA levels of BNIP3 and BNIP3L were determined using qRT-PCR analysis 48 h after HCC-SR cells were cultured under normoxia or hypoxia (1% O2). C The overexposed images of the Immunoblotting analysis...

Additional file 2. Figure S2. Effects of BNIP3 and BNIP3L on autophagic flux in HCC-SR cells. A The levels of BNIP3, BNIP3L, LC3, and β-actin in HCC-SR cells were determined using immunoblotting analysis. The LC3-II／LC3-I was labelled below the LC3 lane. B The expression of GFP-LC3 was determined using confocal microscopy analysis. The results suggested that overexpressing BNIP3 and BNIP3L enhanced the autophagy flux of HCC-SR cells. Scale bar: 10 µm. Cells in A and...

Additional file 4.

Additional file 8: Supplementary Table 2. down-regulated genes.

Additional file 7: Figure S7. Representative FACS plots and bar graphs showing CD45R/B220+ B cells in the spleens of B-NDG mice in non-IR + PBS, IR + PBS, IR + GA, IR-OEa8 + PBS, and IR-OEa8 (PM69) + GA groups.

Additional file 1: Fig. S1. Analysis of microglial and astrocyte population after separation. A Flow cytometry assay results of cells in the lower layer. B Flow cytometry assay results of cells in the upper layer.

Additional file 2: Figure S2. The standard IHC PD L1 scores in Adeno-NEPC.

Additional file 3: Figure S3. Co expression of CgA, SYN, CD56 and NSE in Adeno-NEPC.

Additional file 2: Figure S2. Variant allele frequency and copy number change in three patient cohorts. a Variant allele frequency (VAF) of EGFR and MET TKD mutations in baseline patients. b The copy number of MET amplification before and after MET-TKI treatment. c VAF of MET exon 14 skipping alterations (METex14) before and after MET-TKI treatment. d VAF of EGFR and MET TKD mutations in patients before and after EGFR-TKI treatment.

Additional file 1: Figure S1. (A) 143B cells were treated with 3 μM TM for 0, 24 and 36 h. The expression levels of LINC00629 were detected by qRT-PCR. (B) Kaplan–Meier plot of the overall survival rate of 259 patients with sarcoma. The data were obtained from the Kaplan-Meier Plotter. (C) LINC00629 was knocked down in MNNG/HOS and143B cells using lentivirus expressing shRNAs. The expression of LINC00629 was detected by qRT-PCR. (D-E) 143B cells with...

Additional file 2: Figure S2. (A-B) The osteosarcoma cells (3000 cell/well) with or without LINC00629 overexpression were tested for the cell growth in the colony formation assay. Viable colonies after 1 week were counted and were shown(A). Data are depicted as bar graphs (B). (C-D) The migration of the indicated cells was detected by Transwell assays. Represented images of crystal violet-stained culture plates were shown (C). Data are depicted as bar graphs (D). Data in...

Additional file 4: Figure S4. (A-B) 143B cells with or without LINC00629 knockdown were treated with 10 mg/ml cycloheximide (CHX) for the indicated times. The expression levels of KLF4 were detected by Western blot (A) and quantification of KLF4 levels relative to GAPDH is shown (B). Results are shown as mean ± s.d. n = 3 independent experiments. P = 0.003. (C) The 143B cells with or without LINC00629 knockdown were transfected with the indicated...

Additional file 6: Figure S6. (A-B) The osteosarcoma cells (3000 cell/well) with or without KLF4 knockdown were tested for the cell growth in the colony formation assay. Viable colonies after 1 week were counted and were shown (A). Data are depicted as bar graphs (B). (C-D) The migration of the indicated cells was detected by transwell assays. Represented images of crystal violet-stained culture plates were shown (C). Data are depicted as bar graphs (D). (E-F)...

Additional file 4: Figure S4. MTT assay of ZIF-90-RhB and ZIF-90-RhB-GW2580. (a and b) The cell viabilities of (a) ZIF-90-RhB and (b) ZIF-90-RhB-GW2580 at different concentrations from 3.125 to 50 mg/L.

Additional file 4: Figure S4. MTT assay of ZIF-90-RhB and ZIF-90-RhB-GW2580. (a and b) The cell viabilities of (a) ZIF-90-RhB and (b) ZIF-90-RhB-GW2580 at different concentrations from 3.125 to 50 mg/L.

Additional file 2: Fig. S2. Combination of FPR1 and FPR2 inhibitors improves inflammation, but efficacy improvement is not significant. (A, B) Kidney tissues were sectioned for histological examination. Scale bar, 100 μm. Representative images from one experiment out of three are shown. (C) Serum from sham surgery, IRI, WRW4-treated, and CsH/WRW4-treated rats was sampled at 24 h after surgery. Serum creatinine and blood urea nitrogen levels were measured. (D) MDA levels in rat kidneys were...

Additional file 4: Fig. S4. Identification of optimal MOI of lentiviruses with GFP.

Additional file 4: Fig. S4. Identification of optimal MOI of lentiviruses with GFP.

Additional file 5: Fig. S5. Cell survival of astrocytes in hydrogel detected by CCK-8 test reagent.