847 Works

Circular RNA circCHFR downregulation protects against oxidized low-density lipoprotein-induced endothelial injury via regulation of microRNA-15b-5p/growth arrest and DNA damage inducible gamma

Yang Li & Bing Wang
Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

Melatonin affects hypoxia-inducible factor 1α and ameliorates delayed brain injury following subarachnoid hemorrhage via H19/miR-675/HIF1A/TLR4

Zhijian Xu, Fengduo Zhang, Hu Xu, Fan Yang, Gezhi Zhou, Minfeng Tong, Yaqiang Li & Song Yang
This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Hyperthermia inhibits growth of nasopharyngeal carcinoma through degradation of c-Myc

Xiaole Li, Shichao Duan, Yingjuan Zheng, Yongqiang Yang, Lei Wang, Xinqiang Li, Qing Zhang, Rick F. Thorne, Wencai Li & Daoke Yang
Hyperthermia is a widely used adjunct treatment for different cancers including nasopharyngeal carcinoma (NPC). The protooncogene c-Myc is up-regulated in NPC and its expression is associated with poor prognosis. We hypothesized that c-Myc constitutes an important hyperthermia treatment target, and we investigated its contribution to hyperthermia responses in NPC. The growth of the human NPC cell lines CNE1 and CNE2 was analyzed using CCK-8 and clonogenicity assays after 43 °C hyperthermia, knockdown or overexpression of...

Modified Taohong Siwu decoction improves cardiac function after myocardial ischaemia and reperfusion in rats by promoting endogenous stem cell mobilization and regulating metabolites

Wan-ting Meng, Zhong-Xin Xiao, Han Li, Ya-chao Wang, Yue Zhao, Yan Zhu & Hai-dong Guo
Taohong Siwu decoction (THSWD) has been shown to promote heart repair in myocardial infarction. To determine the effects of modified THSWD (THSWD plus four ingredients) on myocardial ischaemia and reperfusion (I/R) injury. Sixty Sprague-Dawley rats were randomly divided into the I/R group and three different modified THSWD dose groups (gavage administration, 1.215, 2.43, and 4.86 g, respectively). 2,3,5-Triphenyltetrazolium chloride and Evans blue staining were used to detect the infarct area at 24 h after treatment....

Additional file 2 of SPARC plays an important role in the oviposition and nymphal development in Nilaparvata lugens Stål

Weixia Wang, Tingheng Zhu, Pinjun Wan, Qi Wei, Jiachun He, Fengxiang Lai & Qiang Fu
Additional file 2: Figure. S1. Unrooted phylogenetic tree of NlSPARC from N.lugens and representative insect species. An unrooted phylogenetic tree was constructed by the neighbour-joining tree construction program Mega 7. Evolutionary distances were computed using Poisson correction method. Branch support values (1000 bootstraps) for nodes are indicated only support values > 50% are shown. NlSPARC is marked with filled triangle. All protein sequences (accession numbers, length and pI) obtained from GenBank was listed in Table...

Additional file 1 of ETV2 promotes osteogenic differentiation of human dental pulp stem cells through the ERK/MAPK and PI3K-Akt signaling pathways

Jing Li, Haoran Du, Xin Ji, Yihan Chen, Yishuai Li, Boon Chin Heng & Jianguang Xu
Additional file 1: Figure S1. The mRNA level of CBFA1 in qRT-PCR (a) and RNA-Seq (b). *P < 0.05.

Additional file 4 of ETV2 promotes osteogenic differentiation of human dental pulp stem cells through the ERK/MAPK and PI3K-Akt signaling pathways

Jing Li, Haoran Du, Xin Ji, Yihan Chen, Yishuai Li, Boon Chin Heng & Jianguang Xu
Additional file 4: Figure S4. HE (scale bar = 50 μm) and Masson (scale bar = 50 μm) staining were performed to evaluate ectopic bone formation in nude mice (n=4).

Ultrasound targeted microbubble destruction-mediated SOCS3 attenuates biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells

Xiaojiang Tang, Na Hao, Yuhui Zhou & Yang Liu
SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Additional file 4 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 4: Figure S1. Validation of ω-transaminase activity based on color reaction. 1: Control 2: Empty vector plasmid strain breaking solution 3: Breaking solution of strain expressing He-ωTA protein 4: Breaking solution of strain expressing Cv-ωTA protein 5: Purified He-ωTA protein solution 6: Purified Cv-ωTA protein solution.

Additional file 5 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 6 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Porcine epidemic diarrhoea virus (PEDV) infection activates AMPK and JNK through TAK1 to induce autophagy and enhance virus replication

Jingxiang Wang, Xianjin Kan, Xiaomei Li, Jing Sun & Xiulong Xu
Autophagy plays an important role in defending against invading microbes. However, numerous viruses can subvert autophagy to benefit their replication. Porcine epidemic diarrhoea virus (PEDV) is an aetiological agent that causes severe porcine epidemic diarrhoea. How PEDV infection regulates autophagy and its role in PEDV replication are inadequately understood. Herein, we report that PEDV induced complete autophagy in Vero and IPEC-DQ cells, as evidenced by increased LC3 lipidation, p62 degradation, and the formation of autolysosomes....

Knockdown of lectin-like oxidized low-density lipoprotein-1 ameliorates alcoholic cardiomyopathy via inactivating the p38 mitogen-activated protein kinase pathway

Yifan Zhang, Ruiqi Zhang, Lan Lu, Na Zhou, Xiaoyan Lv, Xin Wang & Zhanbin Feng
LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

Additional file 2 of Combining QTL mapping and RNA-Seq Unravels candidate genes for Alfalfa (Medicago sativa L.) leaf development

Xueqian Jiang, Xijiang Yang, Fan Zhang, Tianhui Yang, Changfu Yang, Fei He, Ting Gao, Chuan Wang, Qingchuan Yang, Zhen Wang & Junmei Kang
Additional file 2: Fig. S2 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of maternal parent (P2).

Additional file 1 of Comparison of open, laparoscopic, and robotic left colectomy for radical treatment of colon cancer: a retrospective analysis in a consecutive series of 211 patients

Zhixiang Huang, Taiyuan Li, Genghua Zhang, Zhen Zhou, Haoran Shi, Cheng Tang, Lingling Yang & Xiong Lei
Additional file 1: Supplementary Figure 1. Illustrations for the length of bowel resection, location of related arteries ligation and scope of the lymph node dissection for radical left colectomy for tumours located (A) at the distal 1/3 of the transverse colon (TC) and splenic flexure (SF), (B) at the upper segment of descending colon (UDC), (C) at the lower segment of descending colon (LDC).

Additional file 5 of Exosomal circZNF451 restrains anti-PD1 treatment in lung adenocarcinoma via polarizing macrophages by complexing with TRIM56 and FXR1

Jian Gao, Yong-Qiang Ao, Ling-Xian Zhang, Jie Deng, Shuai Wang, Hai-Kun Wang, Jia-Hao Jiang & Jian-Yong Ding
Additional file 5: Supplementary Figure 1. A The enrichment of 4 most significantly changed circRNAs (FC > 4, P < 0.05) of the circRNA sequencing in the exosomes of 6 LUAD patients (3 PR and 3 PD) accepting the PD1 blockade was measured by qRT-PCR. B Correlation between exosomal circRNA-seq results of circZNF451 and the expression of circZNF451 in the tumor tissues of six LUAD patients was analyzed by Spearman correlation analysis. C The expression...

Additional file 6 of Exosomal circZNF451 restrains anti-PD1 treatment in lung adenocarcinoma via polarizing macrophages by complexing with TRIM56 and FXR1

Jian Gao, Yong-Qiang Ao, Ling-Xian Zhang, Jie Deng, Shuai Wang, Hai-Kun Wang, Jia-Hao Jiang & Jian-Yong Ding
Additional file 6: Supplementary Figure 2. A Infiltration of CD4+T, CD8+T, Foxp3+Treg, CD56+NK and CD11C+dendritic cells and CD86+ and CD163+ macrophages in the circZNF451high and circZNF451low group was presented in the violin plot. B The transfection efficiency of circZNF451 in LLC was measured by qRT-PCR. C The construction of A549-shcircZNF451, H1299-shcircZNF451, H1395-circZNF451 and H1975-circZNF451 cell lines was confirmed by qRT-PCR. D IFN-γ and GZMB levels in the supernatant of CD8+ T cells were measured via...

Additional file 8 of Exosomal circZNF451 restrains anti-PD1 treatment in lung adenocarcinoma via polarizing macrophages by complexing with TRIM56 and FXR1

Jian Gao, Yong-Qiang Ao, Ling-Xian Zhang, Jie Deng, Shuai Wang, Hai-Kun Wang, Jia-Hao Jiang & Jian-Yong Ding
Additional file 8: Supplementary Figure 4. A After coculturing with A549 for 48h, RNA pull down was performed in macrophages transfected with TRIM56mutant to confirm the interaction between circZNF451 and TRIM56mutant. B The co-IP assay was used to verify the interaction between FXR1 and TRIM56 in macrophage after coculturing with A549/H1299-shcircZNF451 cells for 48h. C The M2 phenotype markers Arg1 and IL-10 were detected by qRT-PCR in LPS stimulated macrophage-siFXR1 cells stimulated by LPS. D...

Additional file 3 of Integrated assessment of the clinical and biological value of ferroptosis-related genes in multiple myeloma

Bibo Fu, Ruonan Shao, Huizhong Wang, Guanjun Chen, Shenrui Bai & Hua Wang
Additional file 3: Figure S2. Genetic alterations of the 11 FRGs in CCLE.

Additional file 1 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 1: Figure S1. (A) WT-HeLa cells were treated with 20 μM of J3 for 3, 6, and 12 h for western blot, quantification of the protein expression of p62 (B) and LC3 (C) was analyzed. (D) GFP-LC3-HeLa were treated as (A), GFP-LC3 dots were analyzed. Scale bar = 20 μm. (E) GFP-LC3 dots of (D) were quantified. (F) GFP-LC3-MEF and GFP-LC3-A549 cells were incubated with 20 μM of J3 for 6 h. GFP-LC3...

Additional file 2 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 2: Figure S2. WT-HeLa cells were incubated with 20 μM of J3 with or without 2 mM of NAC, 1 μM of compound C, 10 μM of U0126, 10 μM of BAPTA for 6 h, respectively. The protein level of LC3-II was detected by western blot and quantified.

Additional file 5 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 5: Figure S5. (A) The flow diagram of the mice generation, genotype identification, and the administration methods (HdhQ140/Q140 and WT mice are not littermates in our study). (B) Dissection of the mice striatum were shown in the picture, the red line indicates the outline for a piece of striatum. (C) The immunostaining of T-HTT (anti-HTT, MAB2166) in the striatum of WT and homozygous mice was analyzed at 12-month-old. (D) The expression of total...

Additional file 5 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 5: Figure S5. (A) The flow diagram of the mice generation, genotype identification, and the administration methods (HdhQ140/Q140 and WT mice are not littermates in our study). (B) Dissection of the mice striatum were shown in the picture, the red line indicates the outline for a piece of striatum. (C) The immunostaining of T-HTT (anti-HTT, MAB2166) in the striatum of WT and homozygous mice was analyzed at 12-month-old. (D) The expression of total...

Additional file 6 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 6: Figure S6. (A-B) Analysis of the expression level of T-HTT (anti-HTT, MAB2166) and DARPP32 in the striatum of 15-month-old homozygous HdhQ140/Q140 mice with or without 60 mg/kg of J3 administration for 3 months. Data are presented as mean ± sem from three individual samples. *p < 0.05, **p < 0.01, ***p < 0.001. (C) Values of parameters characterizing physicochemical and basic ADME properties calculated using ACD/Percepta.

Additional file 3 of Costunolide is a dual inhibitor of MEK1 and AKT1/2 that overcomes osimertinib resistance in lung cancer

Xueli Tian, Rui Wang, Tingxuan Gu, Fayang Ma, Kyle Vaughn Laster, Xiang Li, Kangdong Liu, Mee-Hyun Lee & Zigang Dong
Supplementary Material 3

Registration Year

  • 2022
    847

Resource Types

  • Image
    847

Affiliations

  • Fudan University
    847
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    571
  • Zhejiang University
    563
  • Sichuan University
    518
  • Huazhong University of Science and Technology
    499
  • Shanghai Jiao Tong University
    485
  • Sun Yat-sen University
    484
  • Nanjing Medical University
    470
  • Capital Medical University
    456
  • Chinese Academy of Sciences
    405