Additional file 10. Figure S10. (a) T-Distributed Stochastic Neighbor Embedding (t-SNE) plot depicting clusters of all immune and non-immune cells from 24 EMT-6 tumors analyzed by 10× genomics scRNA-seq. (b) Violin plot showing expression of Ptprc (encoding CD45) in all clusters from EMT-6 tumors.

Additional file 4. Figure S4: The effects of MSA-2 and TGF-β on chemokine production in BMDM. (a-f) Unactivated BMDMs were cultured with MSA-2 for one day, and supernatants were collected for chemokine detection.

Additional file 5. Figure S5: MSA-2-stimulated IFN-β expression in cancer cells. Three cell lines B16, CT26, and EMT-6 were cultured with MSA-2 for one day, and supernatants were collected for IFN-β detection with ELISA. BMDM and BMDC were used as positive controls.

Additional file 3: Figure S3. Significantly regulated PI3K pathway genes in HeLa cells 2 h after 4 Gy irradiation compared to unirradiated cells

Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant chemotherapy treatment predicts survival. Studies have shown that patient-derived organoids can be used in cancer research and drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples to predict the response of patients to neoadjuvant chemotherapy. We generated a living organoid biobank from locally advanced breast cancer patients receiving neoadjuvant chemotherapy. When...

Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant chemotherapy treatment predicts survival. Studies have shown that patient-derived organoids can be used in cancer research and drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples to predict the response of patients to neoadjuvant chemotherapy. We generated a living organoid biobank from locally advanced breast cancer patients receiving neoadjuvant chemotherapy. When...

Additional file 1: Other immune cell populations besides MAIT cells in grafts on aGVHD development.

Additional file 1: Other immune cell populations besides MAIT cells in grafts on aGVHD development.

Additional file 1: Figure S1. The relative abundance of P-CA regulators in DLAT wild and DLAT mutation groups is presented

Additional file 3: Figure S3. TPM1 knockdown reduces LPS-induced inflammation and function decline in C57BL/6J mice. A, B mRNA level of TPM1 (A) and IL-10 (B) in C57BL/6 J mouse retinas after treatments with LPS and siCTR or siTPM1-1. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to LPS + siCTR or LPS + siTPM1-1, *p < 0.05, **p < 0.01). n = 4 or...

Additional file 7: Figure S7. (A) Phylogenetic tree of CEF3 in rice and Arabidopsis. (B) The amino acid sequence alignment between the AtSCD2 and OsCEF3. Different rectangles represent different functional domains.

Additional file 2.

Additional file 2: Fig S1. (A) Echocardiography shows transposition of great arteries and ventricular septal defect in the patient JM0087 (F-2: II-1). (B) Chest X-ray shows dextrocardia in the patient JM0529 (F-4:II-1) at the age of 2 years

Additional file 1: Figure S1.Changes in the metabolic indicators in each group of mice during the experiment. a In step 1 of the animal experiment, the blood glucose level of diabetic mice increased significantly after modelling. It then showed a downward trend in the later stage of the experiment but remained higher than 16.7 mmol/L (random blood glucose). b Changes in the body weight of mice in step 1 of the animal experiment. c Changes...

Additional file 1: Figure S1.Changes in the metabolic indicators in each group of mice during the experiment. a In step 1 of the animal experiment, the blood glucose level of diabetic mice increased significantly after modelling. It then showed a downward trend in the later stage of the experiment but remained higher than 16.7 mmol/L (random blood glucose). b Changes in the body weight of mice in step 1 of the animal experiment. c Changes...

Additional file 1: Figure S1. The increase rate of frequency by slope from low to high. The increase rate of frequency was defined as the frequency at a specific slope was divided by the accumulated frequency at all observed slopes that were less than the specific slope.

Supplementary Material 9

Additional file 6. Figure S6: The effect of the combination therapy on T cell infiltration and peritumoral collagen deposition in the EMT-6 model. (a) Immunofluorescent staining showing T cells in tumor margin and center. (b) Picrosirius red staining showing picrosirius red staining.

Additional file 9. Figure S9: The effect of the combination therapy on proliferation and apoptosis markers in B16 and EMT-6 model. (a) Tunel staining in B16 model. (b) Ki67 staining in B16 model. (c) Tunel staining in EMT-6 model. (d) Ki67 staining in EMT-6 model. (e) PCNA staining in EMT-6 model.

Additional file 4: Fig S4. Schematic illustration of DSB induction and homologous recombination. After preparing competent cells, the pTarget-gene and Donor DNA which harbored homology arms (HRR and HRL) that targeted a chromosomal locus spanning the middle of targeted gene and the DSB site were electroporated into cells.

Additional file 4: Fig S4. Schematic illustration of DSB induction and homologous recombination. After preparing competent cells, the pTarget-gene and Donor DNA which harbored homology arms (HRR and HRL) that targeted a chromosomal locus spanning the middle of targeted gene and the DSB site were electroporated into cells.

sj-tif-7-cll-10.1177_09636897221129171 for Effects of Low-Intensity Pulsed Ultrasound on the Migration and Homing of Human Amnion–Derived Mesenchymal Stem Cells to Ovaries in Rats With Premature Ovarian Insufficiency by Li Ling, Jiying Hou, Yan Wang, Han Shu and Yubin Huang in Cell Transplantation

Additional file 2: Supplementary figure 2. The relationship between immune-related function and prognosis of CLL patients.

Supplemental material, sj-tif-5-tej-10.1177_20417314221132093 for A new cell-free therapeutic strategy for liver regeneration: Human placental mesenchymal stem cell-derived extracellular vesicles by Ting Li, Yu Fu, Zeyi Guo, Honglei Zhu, Hangyu Liao, Xiaoge Niu, Lin Zhou, Shunjun Fu, Yang Li, Shao Li, Lujia Wang, Yizhou Zheng, Lei Feng, Yi Gao and Guolin He in Journal of Tissue Engineering

Additional file 5: Figure S5. TPM1 knockdown changes transcriptome associated with glial cells in WT mouse retina. A A heatmap showing DEGs associated with microglia and astrocytes in WT mice following treatments with PBS, or with LPS and siTPM1-1 or siCTR. B–G The expression levels of DEGs associated with M1 (B) or M2 microglia (C, D) and with A1 (E) or A2 astrocytes (F, G) in WT mice after treatment with PBS, or with LPS...