Mitochondrial injury-triggered podocyte apoptosis is a major risk factor for diabetic nephropathy (DN). However, the detailed relationship between mitochondrial homeostasis and podocyte apoptosis remains unclear. The present study aimed to explore the role and functional mechanism of germacrone in DN in type I diabetes (type I DN). A mouse model of type I DN was established by injecting streptozocin, and a podocyte injury model was constructed using high glucose (HG) induction. Histopathology was detected by...

Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

Numerous studies have reported the regulatory effects of miR-21-5p and reversine in human breast cancer (HBC). However, the mechanism of reversine and miR-21-5p has not been fully investigated in HBC. The aim of the current study was to assess the mechanism of action of reversine, with or without miR-21-5p, in HBC progression. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot results confirmed the upregulation of miR-21-5p and downregulation of sprouty RTK signaling antagonist 2...

Additional file 2: Figure. S1. Unrooted phylogenetic tree of NlSPARC from N.lugens and representative insect species. An unrooted phylogenetic tree was constructed by the neighbour-joining tree construction program Mega 7. Evolutionary distances were computed using Poisson correction method. Branch support values (1000 bootstraps) for nodes are indicated only support values > 50% are shown. NlSPARC is marked with filled triangle. All protein sequences (accession numbers, length and pI) obtained from GenBank was listed in Table...

Additional file 1: Figure S1. The mRNA level of CBFA1 in qRT-PCR (a) and RNA-Seq (b). *P < 0.05.

Additional file 4: Figure S4. HE (scale bar = 50 μm) and Masson (scale bar = 50 μm) staining were performed to evaluate ectopic bone formation in nude mice (n=4).

SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Additional file 4: Figure S1. Validation of ω-transaminase activity based on color reaction. 1: Control 2: Empty vector plasmid strain breaking solution 3: Breaking solution of strain expressing He-ωTA protein 4: Breaking solution of strain expressing Cv-ωTA protein 5: Purified He-ωTA protein solution 6: Purified Cv-ωTA protein solution.

Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Sponges are animals that inhabit many aquatic environments while filtering small particles and ejecting metabolic wastes. They are composed of cells in a bulk extracellular matrix, often with an embedded scaffolding of stiff, siliceous spicules. We hypothesize that the mechanical response of this heterogeneous tissue to hydrodynamic flow influences cell proliferation in a manner that generates the body of a sponge. Towards a more complete picture of the emergence of sponge morphology, we dissected a...

Sponges are animals that inhabit many aquatic environments while filtering small particles and ejecting metabolic wastes. They are composed of cells in a bulk extracellular matrix, often with an embedded scaffolding of stiff, siliceous spicules. We hypothesize that the mechanical response of this heterogeneous tissue to hydrodynamic flow influences cell proliferation in a manner that generates the body of a sponge. Towards a more complete picture of the emergence of sponge morphology, we dissected a...

Additional file 5: Supplementary Figure 1. A The enrichment of 4 most significantly changed circRNAs (FC > 4, P < 0.05) of the circRNA sequencing in the exosomes of 6 LUAD patients (3 PR and 3 PD) accepting the PD1 blockade was measured by qRT-PCR. B Correlation between exosomal circRNA-seq results of circZNF451 and the expression of circZNF451 in the tumor tissues of six LUAD patients was analyzed by Spearman correlation analysis. C The expression...

Additional file 6: Supplementary Figure 2. A Infiltration of CD4+T, CD8+T, Foxp3+Treg, CD56+NK and CD11C+dendritic cells and CD86+ and CD163+ macrophages in the circZNF451high and circZNF451low group was presented in the violin plot. B The transfection efficiency of circZNF451 in LLC was measured by qRT-PCR. C The construction of A549-shcircZNF451, H1299-shcircZNF451, H1395-circZNF451 and H1975-circZNF451 cell lines was confirmed by qRT-PCR. D IFN-γ and GZMB levels in the supernatant of CD8+ T cells were measured via...

Additional file 8: Supplementary Figure 4. A After coculturing with A549 for 48h, RNA pull down was performed in macrophages transfected with TRIM56mutant to confirm the interaction between circZNF451 and TRIM56mutant. B The co-IP assay was used to verify the interaction between FXR1 and TRIM56 in macrophage after coculturing with A549/H1299-shcircZNF451 cells for 48h. C The M2 phenotype markers Arg1 and IL-10 were detected by qRT-PCR in LPS stimulated macrophage-siFXR1 cells stimulated by LPS. D...

Additional file 4: Figure S4. The correlation of pyroptosis-related prognostic genes and clinical features in the training set. (A, B, C) The correlation of NLRP1 with grade, N stage, T stage. (D) The correlation of NLRP2 with grade. (E) The correlation of GSDMC with stage. (F) The correlation of CASP4 with grade. (G) The correlation of risk score with grade.

Additional file 5: Figure S5. GSEA enrichment analysis identifies KEGG pathways associated with high-risk and low-risk groups in the training set (A) and test set (B).

Additional file 9: Figure S9. Overexpression GSDMC promotes cell proliferation and invasion in PAAD. (A)PANC-1 (left panel) and CFPAC-1 (right panel) cells were subjected to immunoblotting as indicated. (B, C) Cell growth of PANC-1 cells (B) and CFPAC-1 cells (C) were measured by CCK-8 assay. *P < 0.05, **P < 0.01 by two-way ANOVA. (D, F) Clone formation of PANC-1 cells (D) and CFPAC-1 cells (F) were measured as indicated ***P < 0.001 by a...

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Additional file 3: Figure S3. Significantly regulated PI3K pathway genes in HeLa cells 2 h after 4 Gy irradiation compared to unirradiated cells

Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant chemotherapy treatment predicts survival. Studies have shown that patient-derived organoids can be used in cancer research and drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples to predict the response of patients to neoadjuvant chemotherapy. We generated a living organoid biobank from locally advanced breast cancer patients receiving neoadjuvant chemotherapy. When...