806 Works

Exosomes from miR-374a-5p-modified mesenchymal stem cells inhibit the progression of renal fibrosis by regulating MAPK6/MK5/YAP axis

Mingzhu Liang, Di Zhang, Danna Zheng, Wenfang He & Juan Jin
Chronic kidney disease (CKD) in clinical is defined as a gradual loss of kidney function for more than 3 months. The pathologic course of CKD is characterized by extensive renal fibrosis; thus, preventing renal fibrosis is vital for the treatment of CKD. It has been reported that microRNA (miR)-374a-5p was under-expressed in renal venous blood samples from patients with CKD. In addition, it exhibited anti-apoptotic effects in renal tissues suggesting that miR-374a-5p may play an...

Circular RNA protein tyrosine kinase 2 (circPTK2) promotes colorectal cancer proliferation, migration, invasion and chemoresistance

Zhipeng Jiang, Zehui Hou, Wei Liu, Zhuomin Yu, Zhiqiang Liang & Shuang Chen
The dysregulated circular RNAs (circRNAs) are linked to progression and chemoresistance in colorectal cancer (CRC). However, the role of circRNA protein tyrosine kinase 2 (circPTK2) in CRC progression and chemoresistance is uncertain. The circPTK2, microRNA (miR)-136-5p, m6A ‘reader’ protein YTH domain family protein 1 (YTHDF1), β-catenin and cyclin D1 abundances were examined via quantitative reverse transcription PCR or Western blotting. The progression was investigated by cell counting kit-8 (CCK-8), colony formation, transwell and xenograft analysis....

Hyperthermia inhibits growth of nasopharyngeal carcinoma through degradation of c-Myc

Xiaole Li, Shichao Duan, Yingjuan Zheng, Yongqiang Yang, Lei Wang, Xinqiang Li, Qing Zhang, Rick F. Thorne, Wencai Li & Daoke Yang
Hyperthermia is a widely used adjunct treatment for different cancers including nasopharyngeal carcinoma (NPC). The protooncogene c-Myc is up-regulated in NPC and its expression is associated with poor prognosis. We hypothesized that c-Myc constitutes an important hyperthermia treatment target, and we investigated its contribution to hyperthermia responses in NPC. The growth of the human NPC cell lines CNE1 and CNE2 was analyzed using CCK-8 and clonogenicity assays after 43 °C hyperthermia, knockdown or overexpression of...

Long non-coding RNA PMS2L2 is down-regulated in osteoarthritis and inhibits chondrocyte proliferation by up-regulating miR-34a

Fei Yang, Min Zhao, Qinghua Sang, Changhong Yan & Zhenjun Wang
Long non-coding RNA (lncRNA) PMS2L2 has been reported to participate in endotoxin-induced inflammatory responses. As these types of responses can promote osteoarthritis (OA), it was of interest to ascertain if PMS2L2 may be involved in OA. To explore any potential participation of PMS2L2 in OA, synovial fluid was extracted from both OA patients and healthy controls (n = 62 each) and PMS2L2 expression of each sample determined by RT-qPCR. In addition, as miR-34a has a...

Additional file 3 of The prognostic value of IPI in patients with primary breast lymphoma, a multicenter retrospective study

Kexin Feng, Shuangtao Zhao, Qingyao Shang, Guangdong Qiao, Jiaxiang Liu, Chenxuan Yang, Ya Wei, Yalun Li, Fei Ren, Lixue Xuan, Xiang Wang & Xin Wang
Additional file 3: Figure S1. ROC curves for 5-year survival of IPI and predictive factors significant in univariate analysis. A, ROC curves for 5-year OS of IPI and predictive factors significant in univariate analysis. B, ROC curves for 5-year PFS of IPI and predictive factors significant in univariate analysis.

Additional file 3 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 3: Fig S2. Origin and evolution of LSSL. (A) The phylogenetic tree for the LSSL family based on the neighbor-joining (NJ) method. An NJ tree was constructed using the amino acid sequences of the LSSL proteins. All species represented by abbreviations are shown in Table S2. The bar (0.020) indicates the genetic distance. Different color lines correspond to different species groups. (B) The motif composition of the LSSL family proteins. (C) Model diagram...

Additional file 2 of A prospective cohort study of Plasmodium falciparum malaria in three sites of Western Kenya

Benyl M. Ondeto, Xiaoming Wang, Harrysone Atieli, Daibin Zhong, Guofa Zhou, Ming-Chieh Lee, Pauline Winnie Orondo, Kevin O. Ochwedo, Collince J. Omondi, Simon M. Muriu, David O. Odongo, Horace Ochanda, James Kazura, Andrew K. Githeko & Guiyun Yan
Additional file 2: Figure S2. Heat map showing the Plasmodium falciparum infection patterns in Kombewa, Iguhu, and Marani in western Kenya.

Additional file 2 of Birth weight is associated with obesity and T2DM in adulthood among Chinese women

Pu Song, Hui Hui, Manqing Yang, Peng Lai, Yan Ye, Ying Liu & Xuekui Liu
Additional file 2. Supplement Figure.

Additional file 2 of PRR34-AS1 promotes exosome secretion of VEGF and TGF-β via recruiting DDX3X to stabilize Rab27a mRNA in hepatocellular carcinoma

Zhilei Zhang, Ye Zhou, Yuming Jia, Chao Wang, Meng Zhang & Zhuo Xu
Additional file 2: Figure S2. DDX3X positively regulates Rab27a expression in HCC cells. A The cellular distribution of PRR34-AS1 in HCC cells was confirmed by subcellular fractionation assay. N = 3. B, C The mRNA and protein levels of DDX3X and Rab27a were analyzed in HCC cells transfected with sh/DDX3X#a/b. N = 3. **P < 0.01. Data were analyzed via DGP PCR quantification.

Additional file 2 of PRR34-AS1 promotes exosome secretion of VEGF and TGF-β via recruiting DDX3X to stabilize Rab27a mRNA in hepatocellular carcinoma

Zhilei Zhang, Ye Zhou, Yuming Jia, Chao Wang, Meng Zhang & Zhuo Xu
Additional file 2: Figure S2. DDX3X positively regulates Rab27a expression in HCC cells. A The cellular distribution of PRR34-AS1 in HCC cells was confirmed by subcellular fractionation assay. N = 3. B, C The mRNA and protein levels of DDX3X and Rab27a were analyzed in HCC cells transfected with sh/DDX3X#a/b. N = 3. **P < 0.01. Data were analyzed via DGP PCR quantification.

Additional file 3 of PRR34-AS1 promotes exosome secretion of VEGF and TGF-β via recruiting DDX3X to stabilize Rab27a mRNA in hepatocellular carcinoma

Zhilei Zhang, Ye Zhou, Yuming Jia, Chao Wang, Meng Zhang & Zhuo Xu
Additional file 3: Figure S3. Transfection efficiency of Rab27a in HCC cells. A Rab27a levels in HCC cells was detected by RT-qPCR and western bot analyses after Rab27a silencing. N = 3. **P < 0.01.

Additional file 1 of Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsis

Bo Cheng, Mengyu Du, Shuxuan He, Lan Yang, Xi Wang, Hui Gao, Haiqing Chang, Wei Gao, Yan Li, Qiang Wang & Yansong Li
Additional file 1: Fig. S1. The gating strategies for the flow cytometry experiments. Data analysis was performed using FlowJo (Ashland, OR). Scatter and staining with the FITC-anti-CD41 and PE-anti-CD40L antibodies were used to gate platelet population. Cells were first gated by regions within a side scatter area (SSC-A) versus forward scatter area (FSC-A) plot, and then through gating those populations in the SSC-A versus FITC-A plots. Activated platelets were defined as FITC-anti-CD41-A positive and PE-anti-CD40L-A...

Additional file 2 of Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsis

Bo Cheng, Mengyu Du, Shuxuan He, Lan Yang, Xi Wang, Hui Gao, Haiqing Chang, Wei Gao, Yan Li, Qiang Wang & Yansong Li
Additional file 2: Fig. S2. KEGG enrichment of differential expressed genes in septic patients compared with healthy person. a The volcano plot was constructed using the fold change values and P-adjust. Red dots indicate upregulated genes; blue dots indicate downregulated genes. b The heatmap of the differential gene expression, where different colors represent trends of gene expression in different tissues. c, d The enriched KEGG signaling pathways were selected to demonstrate the primary biological actions...

Additional file 2 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 2: Supplementary Figure S2. Correlation analysis of the 76 NRGs for CESC (A), OV (B), UCEC (C), and UCS D. Red indicates a positive correlation; blue indicates a negative correlation.

Additional file 2 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 2: Supplementary Figure S2. Correlation analysis of the 76 NRGs for CESC (A), OV (B), UCEC (C), and UCS D. Red indicates a positive correlation; blue indicates a negative correlation.

Additional file 6 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 6: Supplementary Figure S6. Expression values of NRGs in prognostic signature of OV. A-D Expression values of NRGs in TCGA for BACH2 (A), MLKL (B), MYC (C), MYCN (D), and SIRT2 (E). F-J Expression values of NRGs in our cohort for BACH2 (F), MLKL (I), MYC (G), MYCN (H), and SIRT2 (J).

Additional file 7 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 7: Supplementary Figure S7. Clinical value of risk score by independent prognostic analysis. A-H The Univariate Cox regression analysis and Multivariate Cox regression analysis for CESC (A-B), OV (C-D), UCEC (E-F), and UCS G-H.

Additional file 4 of Role of a lipid metabolism-related lncRNA signature in risk stratification and immune microenvironment for colon cancer

Yaobin Lin, Yu Xiao, Shan Liu, Liang Hong, Lingdong Shao & Junxin Wu
Additional file 4: Figure S3. Association of immune checkpoints with Clusters and prognostic risk groups. The expression of immune checkpoints A PD-1, B PD-L1, C CTLA-4, D IDO1, E IDO2, F HAVCR2, and G LAG3 in Clusters 1 and 2. The expressions of H PD-L1, I PD-1, J CTLA-4, K IDO1, L IDO2, M HAVCR2, and N LAG3 in different risk groups. PD-1, programmed cell death 1; PD-L1, programmed cell death ligand 1; CTLA-4, cytotoxic...

Additional file 4 of Variety-Specific Transcriptional and Alternative Splicing Regulations Modulate Salt Tolerance in Rice from Early Stage of Stress

Guihua Jian, Yujian Mo, Yan Hu, Yongxiang Huang, Lei Ren, Yueqin Zhang, Hanqiao Hu, Shuangxi Zhou, Gang Liu, Jianfu Guo & Yu Ling
Additional file 4: Read Counts vs. Reads Length Barplot of all samples.

Additional file 4 of Variety-Specific Transcriptional and Alternative Splicing Regulations Modulate Salt Tolerance in Rice from Early Stage of Stress

Guihua Jian, Yujian Mo, Yan Hu, Yongxiang Huang, Lei Ren, Yueqin Zhang, Hanqiao Hu, Shuangxi Zhou, Gang Liu, Jianfu Guo & Yu Ling
Additional file 4: Read Counts vs. Reads Length Barplot of all samples.

Additional file 6 of Variety-Specific Transcriptional and Alternative Splicing Regulations Modulate Salt Tolerance in Rice from Early Stage of Stress

Guihua Jian, Yujian Mo, Yan Hu, Yongxiang Huang, Lei Ren, Yueqin Zhang, Hanqiao Hu, Shuangxi Zhou, Gang Liu, Jianfu Guo & Yu Ling
Additional file 6: GO analysis of expressed genes in rice.

Additional file 7 of Variety-Specific Transcriptional and Alternative Splicing Regulations Modulate Salt Tolerance in Rice from Early Stage of Stress

Guihua Jian, Yujian Mo, Yan Hu, Yongxiang Huang, Lei Ren, Yueqin Zhang, Hanqiao Hu, Shuangxi Zhou, Gang Liu, Jianfu Guo & Yu Ling
Additional file 7: Biological process enrichment of common and variety-specific regulated genes.

Additional file 4 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 4: Figure sup 4. Transcriptional changes of selected astrocyte-enrichedgenes in apoE-TR mice upon cuprizone-induced demyelination. The CC region of apoE-TR mice treated with normal or CPZ diet was subjected to transcriptomic profiling. Heat map showing transcriptional changes (CPZ vs CTL groups) of selected astrocyte-enriched genes identified from RNA-Seq. The scaled expression value (row Z score) is shown with a blue-red color scheme, denoting red as higher expression, and blue as lower expression.

Additional file 3 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 3: Figure sup 3. Similar levels of astrogliosis were observed in apoE-TR mice upon cuprizone-induced demyelination. The astrogliosis in the CC area of CTL (n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice was examined by immunostaining for GFAP. (A) Representative images of GFAP+astrocytes in the CC area of apoE-TR mice are shown. Scale bar, 100 µm. (B) The morphology of GFAP+astrocytes in apoE-TR mice are shown. Scale bar, 25 µm. (C) The fold change of...

Additional file 2 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 2: Figure sup 2. Lack of effects of cuprizone-induceddemyelination on the blood-brainbarrier integrity among apoE-TR mice. (A) Brain samples ofapoE-TR mice from CPZ-treated group (n=16/genotype) were subjected toimmunofluorescence staining for CLDN5(a tight junction-associated protein) and Glut1 (vascular marker). Representative images of CLDN5 (Green) staining and Glut1 (Red)staining in the CC region of apoE-TR mice are shown. Scale bar, 50 µm. Values are mean ± SEM. One-way ANOVA.N.S. not significant.

Registration Year

  • 2022
    806

Resource Types

  • Image
    806

Affiliations

  • Capital Medical University
    806
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    514
  • Zhejiang University
    496
  • Sichuan University
    476
  • Shanghai Jiao Tong University
    460
  • Fudan University
    456
  • Nanjing Medical University
    452
  • Huazhong University of Science and Technology
    451
  • Chinese Academy of Sciences
    403
  • Sun Yat-sen University
    395