Additional file 5: Figure S5. Composition of splenic immune cells and CD4+ T cell subpopulations in healthy Sigirr +/+ mice and Sigirr −/− mice. (A), Frequencies of major immune populations in spleens between Sigirr +/+ mice (n = 5) and Sigirr −/− mice (n = 5). (B), Absolute number and frequency of granulocytes among 2.5 × 105 live CD45+ leukocytes derived from Sigirr +/+ and Sigirr −/−mice. (C), Representative plots of T cells (CD3+ NK1.1−),...

Additional file 6: Figure S6. Gating strategy used to sort naïve CD4 T cells by flow cytometry. After performing cell surface staining with anti-CD4, CD25, CD62L and CD44 antibodies conjugated with fluorescein, naïve CD4 T cells (After) were sorted from splenic suspensions from Sigirr +/+ mice (Before) according to the CD4+ CD25− CD62L+ CD44− strategy among live lymphocytes.

Additional file 3.

Additional file 3: Fig S2. Origin and evolution of LSSL. (A) The phylogenetic tree for the LSSL family based on the neighbor-joining (NJ) method. An NJ tree was constructed using the amino acid sequences of the LSSL proteins. All species represented by abbreviations are shown in Table S2. The bar (0.020) indicates the genetic distance. Different color lines correspond to different species groups. (B) The motif composition of the LSSL family proteins. (C) Model diagram...

Additional file 5: Fig S4. Glycan selectivity of LSSL assessed by glycan microarrays. (A) LSSL (8 µg/mL) binding to 100 glycan microarray. The concentrations given for the glycan microarray represent those used in the carbohydrate immobilization reaction. Data are presented as the mean ± SDs; (n = 3 technical replicates). (B) Confocal microscopy detection of LSSL binding to yeast. LSSL were incubated with yeast cells (105 cells/well) in PBS for 1 h at room temperature...

Additional file 2: Fig. S1. A Schematic diagram of the genomic locus of CEBPA-DT (www.ncbi.nlm.nih.gov/). B The protein-coding potential of CEBPA-DT predicted by Open reading frame (ORF) Finder software prediction ( https://www.ncbi.nlm.nih.gov/orffinder/ ). C The protein-coding potential of CEBPA-DT predicted by Coding-Potential Assessment Tool ( wlcb.oit.uci.edu/cpat/ ).

Additional file 5: Fig. S4. A Clustering heatmap of significant differentially expressed mRNAs in HCCLM9 cells transfected with negative control or CEBPA-DT siRNAs. B Significantly enriched pathways annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Significantly enriched pathways annotated by the Gene Ontology (GO) database. D Significantly enriched pathways annotated by the Molecular Signatures Database (MSigDB). E The binding sites of hnRNPC and 3’UTR of DDR2 were predicted by PRIdictor database...

Additional file 6: Fig. S5. A-B The expression levels of DDR2 were measured by western blot and RT-qPCR in indicated HCCLM9 and SNU-449 cells transfected with negative control siRNA or siRNAs targeting DDR2. C-D Cell migration capacity of indicated HCCLM9 and SNU-449 cells were measured by scratch wound-healing assays. Scale bar, 100μm. E-F Cell migration and invasion capacity of indicated HCCLM9 and SNU-449 cells were measured by transwell migration and matrigel invasion assays. Scale bar,...

Additional file 5. Collection Proof of Strain RW10569.

Additional file 4: Fig. S4. RT-qPCR analysis for SOX17, BLIMP1, TFAP2C, NANOS3, POU5F1, SOX2 during hPGC differentiation in day 4 embryoids, Related to Fig. 1; n = 3 independent experiments. Data are presented as means ± s.d. Statistical analysis was performed by Student’s t-test (two-sided), compared to WT group, *p < 0.05, **p < 0.01, ***p < 0.001, Related to Fig. 1.

Supplemental material, sj-tif-2-cll-10.1177_09636897221139103 for Low-Dose Anti-Thymocyte Globulin Plus Low-Dose Posttransplant Cyclophosphamide as an Effective Regimen for Prophylaxis of Graft Versus Host Disease After Haploidentical Peripheral Blood Stem Cell Transplantation With Maternal/Collateral Related Donors by Ting Li, Qiaomei He, Jun Yang, Yu Cai, Chongmei Huang, Xiaowei Xu, Huiying Qiu, Jiahua Niu, Kun Zhou, Yin Zhang, Xinxin Xia, Yu Wei, Chang Shen, Xueying Ding, Yin Tong, Liping Wan and Xianmin Song in Cell Transplantation

Supplemental material, sj-tif-3-cll-10.1177_09636897221139103 for Low-Dose Anti-Thymocyte Globulin Plus Low-Dose Posttransplant Cyclophosphamide as an Effective Regimen for Prophylaxis of Graft Versus Host Disease After Haploidentical Peripheral Blood Stem Cell Transplantation With Maternal/Collateral Related Donors by Ting Li, Qiaomei He, Jun Yang, Yu Cai, Chongmei Huang, Xiaowei Xu, Huiying Qiu, Jiahua Niu, Kun Zhou, Yin Zhang, Xinxin Xia, Yu Wei, Chang Shen, Xueying Ding, Yin Tong, Liping Wan and Xianmin Song in Cell Transplantation

Additional file 5: Figure sup 5. Transcriptional changes of selected immuneresponse-related genes in apoE-TR mice upon cuprizone-induced demyelination.RNA was extracted from the CC area of CTL (n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice. (A-F)The expression of immune response-associated genes (i.e., Cst7, Ccl2, Csf1, Csf1r, Serpina3n, and Tnf-α) wasmeasured by real-time PCR. Values are mean ± SEM. Mann-Whitney tests followedby Bonferroni correction for multiple comparisons were used. *P < 0.0167;N.S. not significant.

Supplemental material, sj-png-2-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Additional file 4: Figure S4. Enrichment of differentially expressed genes in Serpine1-overexpressing MLE-12 cells. A, B GO and KEGG enrichment of the upregulated genes. C, D GO and KEGG enrichment of the downregulated genes. Q-value ≤ 0.05 and fold-change ≥ 2 were used as the thresholds for screening the differentially expressed genes.

Additional file 3: Figure S3. Significantly regulated PI3K pathway genes in HeLa cells 2 h after 4 Gy irradiation compared to unirradiated cells

Additional file 2: Figure S2. A Scatter plot showing RNA expression changes of genes in HeLa cells 0.5 h after irradiation compared to unirradiated cells. KEGG B and GO C analyses of differentially expressed genes in HeLa cells 0.5 h after irradiation compared to unirradiated cells

Additional file 2: Figure S2. Validation of the pyroptosis-related prognostic model in test set. (A) The patients were divided into two groups according to the threshold of median risk score. Green represents the low-risk group. Red represents the high-risk group. (B) Kaplan Meier curves showing the overall survival of patients in the high-risk and low-risk groups. (C) Survival status of patients with PAAD in high and low risk groups. Green represents survival. Red represents death....

Additional file 2: Figure S2. Validation of the pyroptosis-related prognostic model in test set. (A) The patients were divided into two groups according to the threshold of median risk score. Green represents the low-risk group. Red represents the high-risk group. (B) Kaplan Meier curves showing the overall survival of patients in the high-risk and low-risk groups. (C) Survival status of patients with PAAD in high and low risk groups. Green represents survival. Red represents death....

Additional file 3: Figure S3. Risk model independent prognostic analysis. (A) Univariate independent prognosis Cox regression analysis of risk score and indicated clinical characteristics. (B) Multivariate independent prognosis Cox regression analysis of risk score and indicated clinical characteristics.

Additional file 4: Figure S4. The correlation of pyroptosis-related prognostic genes and clinical features in the training set. (A, B, C) The correlation of NLRP1 with grade, N stage, T stage. (D) The correlation of NLRP2 with grade. (E) The correlation of GSDMC with stage. (F) The correlation of CASP4 with grade. (G) The correlation of risk score with grade.

Additional file 6: Figure S6. The correlation of immune infiltrating cells with risk scores was determined in the training set. (A) Boxplots represent the level of different types of immune infiltrating cells in high- and low- risk group. (B-F) the correlation between the risk score and immune infiltrating cells was further examined by Spearman correlation analysis. *p < 0.05, ***p < 0.001.

Additional file 7: Figure S7. Kaplan-Meier curves of the prognostic pyroptosis-related genes in the training set. The survival curves of NLRP1 (A), NLRP2 (B), GSDMC (C), IL18 (D), CASP4 (E).

Additional file 8: Figure S8. Restoration of GSDMC rescued the inhibition of cell proliferation, migration and invasion induced by GSDMC silencing. (A) PANC-1 (left panel) and CFPAC-1 (right panel) cells were subjected to immunoblotting as indicated. (B, C) Cell growth of PANC-1 cells (B) and CFPAC-1 cells (C) were measured by CCK-8 assay. *P < 0.05, **P < 0.01 by two-way ANOVA. (D, F) Clone formation of PANC-1 cells (D) and CFPAC-1 cells (F) were...

Supplementary Material 10