The alkaloids of Narcissus tazetta L. var. Chinensis Roem (Amaryllidaceae) have antitumor and antiviral activities. However, the immunopharmacological effects of one of its constituents, pseudolycorine chloride (PLY), have not been reported yet. We evaluated the effect of PLY on myeloid-derived suppressor cells (MDSCs) expansion and differentiation into monocyte-like MDSCs (M-MDSCs) and examined whether PLY alleviates Th17 cell-mediated experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS). In vitro, MDSCs were treated with PLY...

Additional file 7: Figure S3. The box plot of expression data (A) before and (B) after the batch effect correction for the GSE15459. The training-validation expression set was used as reference.

Additional file 4: Figure S4. Forest plot for failure to achieve clinical response (100-point response) at 4 weeks subgroup by previous TNF-α exposure in ADA and control groups.

Additional file 4: Figure S4. Forest plot for failure to achieve clinical response (100-point response) at 4 weeks subgroup by previous TNF-α exposure in ADA and control groups.

Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 2: Figure S2. Forest plot for failure to achieve clinical remission at 4 weeks subgroup by concomitant medication in ADA and control groups.

Additional file 1: Figure S1. Toxicity assessment of PMVs@PLGA complexes on cardiac muscle tissue. A TUNEL apoptosis assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. B BAX IF assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. C BCL-2 IF in cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats (scale bars indicate 100 μm).

Additional file 1: Figure S1. Toxicity assessment of PMVs@PLGA complexes on cardiac muscle tissue. A TUNEL apoptosis assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. B BAX IF assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. C BCL-2 IF in cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats (scale bars indicate 100 μm).

Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

Supplementary Material 1

Additional file 1. Supplementary Fig 1. TRIM46 reduces TBK1 expression and interacts with TBK1 in HEK293T cells. (A) HEK293T cells were transfected with 1μg TBK1-Flag plasmids with 0 μg (-), 1μg (+) and 3 μg (++) TRIM46-Myc plasmids for 24 h, cells were lysed and subjected to western blotting to detect the expression of Flag tag and Myc tag, GAPDH was used as an internal control. (B) HEK293T cells were transfected with TRIM46-Myc plasmids with...

Additional file 3: Fig. S3. Analysis of the methylation data from GEO database. The difference of the methylation level of H2A.Z and SFRP1 between ICC tissue and normal bile duct was visualized by box plot. (A) GSE38860. (B) GSE49656. *P<0.05, **p<0.01, ***p<0.001.

Additional file 2: Figure S2. KEGG enrichment of differential expressed genes in septic patients compared with healthy person. a The volcano plot was constructed using the fold change values and P-adjust. Red dots indicate upregulated genes; blue dots indicate downregulated genes. b The heatmap of the differential gene expression, where different colors represent trends of gene expression in different tissues. c, d The enriched KEGG signaling pathways were selected to demonstrate the primary biological actions...

Additional file 2: Figure S2. KEGG enrichment of differential expressed genes in septic patients compared with healthy person. a The volcano plot was constructed using the fold change values and P-adjust. Red dots indicate upregulated genes; blue dots indicate downregulated genes. b The heatmap of the differential gene expression, where different colors represent trends of gene expression in different tissues. c, d The enriched KEGG signaling pathways were selected to demonstrate the primary biological actions...

Additional file 3: Figure S3. Effects of cilostazol treatment on serum inflammatory factors. The level of serum TNF-α (a) and IL-1β (b) in each group (n = 6). The data are presented as the mean ± SEM, ***P < 0.001, ****P < 0.0001, and ns indicates no significant difference.

Additional file 5: Figure S5. Effect of cilostazol treatment on cardiac function after CLP. a Representative M-mode images of the four different groups. b–e Quantitative analysis of LVEF (b), LVFS (c), LVEDD (d), and LVESD (e) in each group (n = 6). These data indicate no significant difference.

Additional file 7: Figure S7. Effect of LPS and CD40L on TRAFs proteins in EGCs. a Representative western blot images of TRAFs proteins expression in EGCs. b–g Western blot analysis of TRAF1 (b), TRAF2 (c), TRAF3 (d), TRAF4 (e), TRAF5 (f) and TRAF6 (g) expression in different groups of EGCs (n = 6). The data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Supplementary Material 2

Supplementary Material 4

Supplementary Material 4

Additional file 4: Supplementary Fig. S3. TNFAIP2 expression and clinical features of AML patients in the microarray data of GSE14468 from GEO database and Beat AML dataset. (a) Comparison of TNFAIP2 expression level among different subtypes of AML in the distribution of FAB classifications in the microarray data of GSE1446. (b) Comparison of TNFAIP2 expression level in AML patients according to cytogenetic risk stratification in the microarray data of GSE1446. (c-h) Comparison of TNFAIP2 expression...

Additional file 4: Supplementary Fig. S3. TNFAIP2 expression and clinical features of AML patients in the microarray data of GSE14468 from GEO database and Beat AML dataset. (a) Comparison of TNFAIP2 expression level among different subtypes of AML in the distribution of FAB classifications in the microarray data of GSE1446. (b) Comparison of TNFAIP2 expression level in AML patients according to cytogenetic risk stratification in the microarray data of GSE1446. (c-h) Comparison of TNFAIP2 expression...

Additional file 3: Figure S1. Analysis of chromosomal location and gene structure of the CsFLS genes in C. sativa.

Additional file 1: NLRP6 is involved in the recruitment of macrophages and neutrophils in pulmonary infection with P. multocida. WT and Nlrp6-/- mice were intranasally infected with 20 µL P. multocida (1000 CFU) and the same volume of sterilized PBS were used as control. Flow cytometry was used to analyze the recruitment of neutrophils and macrophages after 24 h infection. (A, B, E, F). Aseptic PBS infection group (control group). (C, D, G, H). P....

Additional file 2: Supplemental Figure 2. Situation of affectorcells and target cells (HOS, Saos-2, SW1353) displayed by optical microscopy.