To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Additional file 3.

Additional file 3: Figure S3. Analysis of blood physiology parameters of control, vehicle- and CurE-treated mice. The following blood physiology functions were assayed: WBC: white blood cell count; RBC: red blood cell count; HGB: hemoglobin; MCV: mean corpuscular volume; RDW: red blood cell volume distribution width; PLT: platelet; LYM: lymphocyte count; MON: monocyte count; NEUT: neutrophil count. The data were shown as mean ± standard deviation. N = 6 for each group, **P < 0.01...

Additional file 5: Figure S5. Overexpression of CDK1 enhanced protein stabilities of UBE2C and AURKA/B. a Expressions of UBE2C and AURKA/B in SW-13_NC and SW-13_CDK1 cells after treatment with CHX (100 μmol/L) at 0, 1, 3, 9, 12 h. b Relative expression of UBE2C, AURKA c and AURKB d in SW-13_NC and SW-13_CDK1 cells after treatment with CHX. Statistical differences were assessed by two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.

Additional file 5: Figure S5. Overexpression of CDK1 enhanced protein stabilities of UBE2C and AURKA/B. a Expressions of UBE2C and AURKA/B in SW-13_NC and SW-13_CDK1 cells after treatment with CHX (100 μmol/L) at 0, 1, 3, 9, 12 h. b Relative expression of UBE2C, AURKA c and AURKB d in SW-13_NC and SW-13_CDK1 cells after treatment with CHX. Statistical differences were assessed by two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.

Additional file 6: Figure S6. Silencing the markers of apoptosis or pyroptosis could not rescue CurE-mediated cell death. a Transfection efficacy of knockdown caspase 3 expression in SW-13 cells. b Transfection efficacy of knockdown caspase 3 expression in NCI-H295R cells. c Transfection efficacy of knockdown GSDMD expression in SW-13 cells. b Transfection efficacy of knockdown GSDMD expression in NCI-H295R cells. e Silencing caspase 3 increased necrotic cells triggered by CurE in SW-13 cells. f Silencing...

Additional file 1: NLRP6 is involved in the recruitment of macrophages and neutrophils in pulmonary infection with P. multocida. WT and Nlrp6-/- mice were intranasally infected with 20 µL P. multocida (1000 CFU) and the same volume of sterilized PBS were used as control. Flow cytometry was used to analyze the recruitment of neutrophils and macrophages after 24 h infection. (A, B, E, F). Aseptic PBS infection group (control group). (C, D, G, H). P....

Supplemental material, sj-jpg-1-jbm-10.1177_03936155221132292 for Development and validation of a nomogram involving immunohistochemical markers for prediction of recurrence in early low-risk endometrial cancer by Wei Kong, Yuan Tu, Peng Jiang, Yuzhen Huang, Jingni Zhang, Shan Jiang, Ning Li and Rui Yuan in The International Journal of Biological Markers

Additional file 2: Figure S2. Genes expression levels in lung tissues of neonatal mice (A) The mRNA expression of differentially expressed genes analysed using RT-qPCR in lung tissues of neonatal mice. B, C Nr4a1 expression analysed using western blot in lung tissues of neonatal mice. D Serpine1 expression in lung tissues of neonatal mice detected using RT-qPCR on P1, P7, and P21. E, F Serpine1 expression analysed using RT-qPCR and western blot in siRNA-treated neonatal...

Additional file 3: Figure S3. Quantitative analysis of FTH expression in Fig. 3B.

Additional file 7: Fig. S7. Mapchart shown the distribution of GRAS genes on 7 chromosomes in Chinese rosa.

The aim of this study was to evaluate the association between recurrence-free survival (RFS) and perirenal fat thickness (PFT) in a cohort of Chinese population with unilateral nephrolithiasis. We retrospectively reviewed the medical records of 81 patients with unilateral nephrolithiasis in our center from January 2019 to June 2019. PFT measured on computed tomography (CT) scans was evaluated. Kaplan-Meier curves and log-rank tests were used to assess significant differences in RSF between high-PFT and low-PFT...

Additional file 2: Fig. S2. The number and rough ratio of incidence, prevalence, death and dalys in men and women worlwide from 1990 TO 2019.

Additional file 5: Fig. S5. Trends in the 5 risk factors attributed to AF/AFL in each of the 5 SDI regions, from 1990 to 2019.

Additional file 2: Supplementary Figure S2. Correlation analysis of the 76 NRGs for CESC (A), OV (B), UCEC (C), and UCS D. Red indicates a positive correlation; blue indicates a negative correlation.

Additional file 2: Supplementary Figure S2. Correlation analysis of the 76 NRGs for CESC (A), OV (B), UCEC (C), and UCS D. Red indicates a positive correlation; blue indicates a negative correlation.

Additional file 6: Supplementary Figure S6. Expression values of NRGs in prognostic signature of OV. A-D Expression values of NRGs in TCGA for BACH2 (A), MLKL (B), MYC (C), MYCN (D), and SIRT2 (E). F-J Expression values of NRGs in our cohort for BACH2 (F), MLKL (I), MYC (G), MYCN (H), and SIRT2 (J).

Additional file 7: Supplementary Figure S7. Clinical value of risk score by independent prognostic analysis. A-H The Univariate Cox regression analysis and Multivariate Cox regression analysis for CESC (A-B), OV (C-D), UCEC (E-F), and UCS G-H.

Additional file 2: Fig. S1. The correlation between the lactate metabolism-related gene signatures and GSVA scores of 50 cancer hallmark pathways.

Additional file 12: Figure S4. Representative immunohistochemical staining images for GSTT1 in bone marrows of Control, Model, S+Rg, S, and Rg groups.

Additional file 1: Figure S1. HPLC analysis of SMI from different batches. (A) HPLC analysis of SMI (Lot No. 1907018). (B) HPLC analysis of SMI (Lot No. 1907229). (C) HPLC analysis of SMI (Lot No. 1907277).

Supplemental material, sj-tif-4-onc-10.1177_11795549221127161 for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery by Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang and Rui Yuan in Clinical Medicine Insights: Oncology

Supplemental material, sj-tif-4-onc-10.1177_11795549221127161 for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery by Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang and Rui Yuan in Clinical Medicine Insights: Oncology

Supplemental material, sj-tif-5-onc-10.1177_11795549221127161 for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery by Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang and Rui Yuan in Clinical Medicine Insights: Oncology

Supplemental material, sj-tif-5-onc-10.1177_11795549221127161 for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery by Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang and Rui Yuan in Clinical Medicine Insights: Oncology