Hydrostatic pressure is known to regulate bovine nucleus pulposus cell metabolism, but its mechanism in human nucleus pulposus cells (HNPCs) remains obscure, which attracts our attention and becomes the focus in this study. Specifically, HNPCs were treated with SKL2001 (an agonist in the Wnt/β-catenin pathway) or XAV-939 (an inhibitor of the Wnt/β-catenin pathway), and pressurized under the hydrostatic pressure of 1, 3 and 30 atm. The viability, apoptosis and proteoglycan synthesis of treated HNPC were...

Additional file 1: Figure S1. Panx 2 and Panx 3 mRNA expression after CCI. A-B. A qPCR assay was used to detect Panx 2 (A) and Panx 3 (B) mRNA levels at 4 and 14 days post-CCI. The data are mean ± SEM. n = 3 mice/group. **p < 0.01, ***p < 0.001, vs. sham injury. The data were analyzed by one-way ANOVA followed by Dunnett’s test.

SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Additional file 4: Figure S1. Validation of ω-transaminase activity based on color reaction. 1: Control 2: Empty vector plasmid strain breaking solution 3: Breaking solution of strain expressing He-ωTA protein 4: Breaking solution of strain expressing Cv-ωTA protein 5: Purified He-ωTA protein solution 6: Purified Cv-ωTA protein solution.

Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Additional file 2: Fig. S2 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of maternal parent (P2).

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Additional file 3: Figure S1. The ML phylogenetic tree based on the sequences of the 18S rRNA gene (region 1) with reference sequences from NCBI (Accession number: LN484108.1 and U48380.1) and Dasyhelea sp. as the outgroup. Confidence values at branches are based on ultrafast bootstrap analysis with 10,000 bootstrap alignments.

Additional file 5: Figure S3. The ML phylogenetic tree based on the 28S rRNA gene sequences with reference sequences from NCBI (Accession number: LN484108.1) and Forcipomyia pluvialis as the outgroup. Confidence values at branches are based on ultrafast bootstrap analysis with 10,000 bootstrap alignments.

Additional file 3: Figure S3. TPM1 knockdown reduces LPS-induced inflammation and function decline in C57BL/6J mice. A, B mRNA level of TPM1 (A) and IL-10 (B) in C57BL/6 J mouse retinas after treatments with LPS and siCTR or siTPM1-1. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to LPS + siCTR or LPS + siTPM1-1, *p < 0.05, **p < 0.01). n = 4 or...

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Additional file 1: Figure S1. Phenotype distribution of root morphology traits of 295 rice varieties under low and high-nitrogen treatments. A, HRL: root length under high-nitrogen treatment. B, LRL: root length under low-nitrogen treatment. C, HRD: root diameter under high-nitrogen treatment. D, LRD: root diameter under low-nitrogen treatment.

Additional file 1: Figure S1.Changes in the metabolic indicators in each group of mice during the experiment. a In step 1 of the animal experiment, the blood glucose level of diabetic mice increased significantly after modelling. It then showed a downward trend in the later stage of the experiment but remained higher than 16.7 mmol/L (random blood glucose). b Changes in the body weight of mice in step 1 of the animal experiment. c Changes...

Additional file 1: Figure S1.Changes in the metabolic indicators in each group of mice during the experiment. a In step 1 of the animal experiment, the blood glucose level of diabetic mice increased significantly after modelling. It then showed a downward trend in the later stage of the experiment but remained higher than 16.7 mmol/L (random blood glucose). b Changes in the body weight of mice in step 1 of the animal experiment. c Changes...

Additional file 1: Figure S1. The increase rate of frequency by slope from low to high. The increase rate of frequency was defined as the frequency at a specific slope was divided by the accumulated frequency at all observed slopes that were less than the specific slope.

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Additional file 7: Figure S7. Schematic representation of the molecular mechanisms underlying IUI-induced lung injury in neonatal mice.

Additional file 14: Figure S7. Characterization of stromal cells in the KUL3 cohort. A. UMAP plot of stromal cells colored by cell cluster and sample origin. B. Heat map of marker genes identified through Seurat. For each cell cluster, cells were down-sampled to 100. C. Differentiation trajectory inferred by Monocle2. Dots represent stromal cells colored by identified cell cluster. D. Heat map plot of the top ranked regulons. E. Dot plot of the correlation between...

Additional file 15: Figure S8. Workflow of this study. Single-cell transcriptomes and bulk RNA-seq data were integrated to fully analyze the complicated relationship between colorectal epithelium and surrounding environment. C4 cells were featured with high invasive potential and related with TAMs and CAFs, and further validated in vitro using IHC and mIHC.

Additional file 8: Figure S1. Cellular landscape of CRC in the KUL3 cohort. A. UMAP plot of 26,268 cells colored by cell cluster, CMS and sample origin. Each dot represents a cell and cellular cluster is annotated with text. B. Proportions of the identified cell clusters distributed across tumor, border and adjacent normal tissues with the relative cell type proportions and total cell numbers. Upper: all cell clusters; lower: immune and stromal cell clusters. C....