11 Works

Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.

Brenda Andrews
Our ability to understand the genotype-to-phenotype relationship is hindered by the lack of detailed understanding of phenotypes at a single-cell level. To systematically assess cell-to-cell phenotypic variability, we combined automated yeast genetics, high-content screening and neural network-based image analysis of single cells, focussing on genes that influence the architecture of four subcellular compartments of the endocytic pathway as a model system. Our unbiased assessment of the morphology of these compartments — endocytic patch, actin patch,...

sj-jpg-2-jhs-10.1177_17531934221126864 - Supplemental material for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly

Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge & Naoki Morimoto
Supplemental material, sj-jpg-2-jhs-10.1177_17531934221126864 for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly by Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge and Naoki Morimoto in Journal of Hand Surgery (European Volume)

sj-jpg-2-jhs-10.1177_17531934221126864 - Supplemental material for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly

Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge & Naoki Morimoto
Supplemental material, sj-jpg-2-jhs-10.1177_17531934221126864 for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly by Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge and Naoki Morimoto in Journal of Hand Surgery (European Volume)

Whole-organ analysis of TGF-beta-mediated remodelling of the tumour microenvironment by tissue clearing

Shimpei I. Kubota
Tissue clearing is one of the most powerful strategies for a comprehensive analysis of disease progression. Here, we established an integrated pipeline that combines tissue clearing, 3D imaging, and machine learning and applied to a mouse tumour model of experimental lung metastasis using human lung adenocarcinoma A549 cells. This pipeline provided the spatial information of the tumour microenvironment. We further explored the role of transforming growth factor-beta (TGF-beta) in cancer metastasis. TGF-beta-stimulated cancer cells enhanced...

sj-jpg-1-jhs-10.1177_17531934221126864 - Supplemental material for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly

Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge & Naoki Morimoto
Supplemental material, sj-jpg-1-jhs-10.1177_17531934221126864 for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly by Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge and Naoki Morimoto in Journal of Hand Surgery (European Volume)

sj-jpg-1-jhs-10.1177_17531934221126864 - Supplemental material for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly

Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge & Naoki Morimoto
Supplemental material, sj-jpg-1-jhs-10.1177_17531934221126864 for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly by Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge and Naoki Morimoto in Journal of Hand Surgery (European Volume)

sj-jpg-4-jhs-10.1177_17531934221126864 - Supplemental material for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly

Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge & Naoki Morimoto
Supplemental material, sj-jpg-4-jhs-10.1177_17531934221126864 for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly by Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge and Naoki Morimoto in Journal of Hand Surgery (European Volume)

Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.

Brenda Andrews
Our ability to understand the genotype-to-phenotype relationship is hindered by the lack of detailed understanding of phenotypes at a single-cell level. To systematically assess cell-to-cell phenotypic variability, we combined automated yeast genetics, high-content screening and neural network-based image analysis of single cells, focussing on genes that influence the architecture of four subcellular compartments of the endocytic pathway as a model system. Our unbiased assessment of the morphology of these compartments — endocytic patch, actin patch,...

sj-jpg-3-jhs-10.1177_17531934221126864 - Supplemental material for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly

Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge & Naoki Morimoto
Supplemental material, sj-jpg-3-jhs-10.1177_17531934221126864 for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly by Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge and Naoki Morimoto in Journal of Hand Surgery (European Volume)

sj-jpg-3-jhs-10.1177_17531934221126864 - Supplemental material for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly

Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge & Naoki Morimoto
Supplemental material, sj-jpg-3-jhs-10.1177_17531934221126864 for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly by Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge and Naoki Morimoto in Journal of Hand Surgery (European Volume)

sj-jpg-4-jhs-10.1177_17531934221126864 - Supplemental material for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly

Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge & Naoki Morimoto
Supplemental material, sj-jpg-4-jhs-10.1177_17531934221126864 for Use of the duplication range concept for understanding morphology and predicting prognosis in thumb polydactyly by Susumu Saito, Aiko Makino, Hiroki Yamanaka, Itaru Tsuge and Naoki Morimoto in Journal of Hand Surgery (European Volume)

Registration Year

  • 2022
    8
  • 2021
    1
  • 2020
    2

Resource Types

  • Image
    11

Affiliations

  • RIKEN
    11
  • Ryukoku University
    8
  • Nihon University
    8
  • Institute for Research in Biomedicine
    2
  • Memorial Sloan Kettering Cancer Center
    2
  • Institute of Biomedical Research of Barcelona
    2
  • Institució Catalana de Recerca i Estudis Avançats
    2
  • University of Toronto
    2
  • Niigata University
    1
  • University of Tokyo
    1