Additional file 1: Supplemental Figure 1. The cytotoxicity of B7-H3CAR-T cells against solid tumor cell lines was analyzed by RTCA assay. The redarrow represents the time point of effector cell addition.

Long non-coding RNA (lncRNA) PMS2L2 has been reported to participate in endotoxin-induced inflammatory responses. As these types of responses can promote osteoarthritis (OA), it was of interest to ascertain if PMS2L2 may be involved in OA. To explore any potential participation of PMS2L2 in OA, synovial fluid was extracted from both OA patients and healthy controls (n = 62 each) and PMS2L2 expression of each sample determined by RT-qPCR. In addition, as miR-34a has a...

Additional file 2: Fig. S2. HDAC3 binds to miR-195 promoter. A The binding of HDAC3 to miR-195 promoter region as detected by ChIP assay on chromatin extract of A498 cells using anti-HDAC3 antibody or control (IgG). B A498 cells were transfected with si-NC or si-HDAC3 for 48 h, and then ChIP assay was performed with antibodies against K27-acetylated histone H3 (anti-acH3) or control (IgG). *p < 0.05 between the two groups. The cell experiment was...

Additional file 1: Figure S1. Identify the ligamentum flavum (LF) cells. A Pictures showing the morphology of the isolated cells with 1st and 3rd passage from LF tissues. B Identification of the phenotype of cultured LF cells using immunofluorescence staining. Immunofluorescence staining for collagen I and Vimentin in cultured cells.

Additional file 4: Figure S4. The SNAI2 inhibits miR-4306 expression to promote the proliferation and fibrosis of HLF cells in vitro. A RT-qPCR was used to detect the miR-4306 expression in HLF cells transfected with vector, miR-4306 inhibitor, and miR-4306 inhibitor + shSNAI2. B The proliferation of HLF cells transfected with vector, miR-4306 inhibitor, and miR-4306 inhibitor + shSNAI2 was determined by CCK-8 assays. C The apoptosis of HLF cells transfected with vector, miR-4306 inhibitor,...

Supplemental material, sj-tif-3-onc-10.1177_11795549221127161 for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery by Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang and Rui Yuan in Clinical Medicine Insights: Oncology

Additional file 10: Figure S10. A.443654 inhibits the cell proliferation of PAAD. (A) Cell viability of PANC-1 cell line treated with DMSO and 50 nM A.443654, respectively. *P < 0.05, **P < 0.01 by a two-tailed unpaired t-test. (B) Colony formation assay of PANC-1 cells treated with DMSO and 50 nM A.443654, respectively. **P < 0.01 by a two-tailed unpaired t-test. (C) Edu assay of PANC-1 cells treated with DMSO and 50 nM A.443654, respectively....

Additional file 1: Figure S1. Functional enrichment analyses of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). (A) Bubble graph for GO enrichment (the bigger bubble means the more genes enriched, and the increasing depth of red means the differences were more obvious; q-value: the adjusted p-value). (B) KEGG enrichment analysis of differentially expressed genes.

Additional file 3: Figure S3. Risk model independent prognostic analysis. (A) Univariate independent prognosis Cox regression analysis of risk score and indicated clinical characteristics. (B) Multivariate independent prognosis Cox regression analysis of risk score and indicated clinical characteristics.

Additional file 6: Figure S6. The correlation of immune infiltrating cells with risk scores was determined in the training set. (A) Boxplots represent the level of different types of immune infiltrating cells in high- and low- risk group. (B-F) the correlation between the risk score and immune infiltrating cells was further examined by Spearman correlation analysis. *p < 0.05, ***p < 0.001.

Additional file 9: Figure S9. Overexpression GSDMC promotes cell proliferation and invasion in PAAD. (A)PANC-1 (left panel) and CFPAC-1 (right panel) cells were subjected to immunoblotting as indicated. (B, C) Cell growth of PANC-1 cells (B) and CFPAC-1 cells (C) were measured by CCK-8 assay. *P < 0.05, **P < 0.01 by two-way ANOVA. (D, F) Clone formation of PANC-1 cells (D) and CFPAC-1 cells (F) were measured as indicated ***P < 0.001 by a...

Supplemental material, sj-jpg-1-jbm-10.1177_03936155221132292 for Development and validation of a nomogram involving immunohistochemical markers for prediction of recurrence in early low-risk endometrial cancer by Wei Kong, Yuan Tu, Peng Jiang, Yuzhen Huang, Jingni Zhang, Shan Jiang, Ning Li and Rui Yuan in The International Journal of Biological Markers

Supplemental material, sj-jpg-3-jbm-10.1177_03936155221132292 for Development and validation of a nomogram involving immunohistochemical markers for prediction of recurrence in early low-risk endometrial cancer by Wei Kong, Yuan Tu, Peng Jiang, Yuzhen Huang, Jingni Zhang, Shan Jiang, Ning Li and Rui Yuan in The International Journal of Biological Markers

Additional file 2: Figure S2. Schematic representation of the sequences of the dual luciferase reporter gene vectors for the 4 miRNAs.

Additional file 3: Figure S3. HE (scale bar = 200 μm) and Masson (scale bar = 50 μm) staining in the rat calvarial defect model (n = 6).

Additional file 1: Figure S1. MICAL1 promoted the progression of PC by activating WNT pathway. A–C Cell proliferation of indicated PC cells processed or untreated with WNT inhibitors were determined by CCK-8(A), colony formation (B) and EdU (C) assays. D, E Cell metastasis and invasion of indicated PC cells processed or untreated with WNT inhibitors were determined by Transwell (D) and wound healing (E) assays. F Crucial proteins of WNT pathway were detected by western...

Additional file 3: Figure S3. phosphorylating TBC1D1 did not affect the transcription of FZD7 A The mRNA level of FZD7 was detected in PC cells when TBC1D1 expression level or phosphorylation level changed.

Additional file 4: Figure S4. TBC1D1 interference inhibited malignant activities could be rescued by FZD7 reestablishment. A–B Cell proliferative capability was determined by CCK-8(A) and EdU (B)assays. C–D Cell metastatic and invasive capability were determined by wound healing (C) and Transwell (D) assays. E TOP/FOP flash assay was conducted on indicate cells to evaluate the effect of FZD7 on WNT/β-catenin signaling. F Western blot assay was conducted on indicate cells to evaluate the effect of...

Additional file 1: Supplementary Figure 1. MMP-13 levels in the medium of IL-1β-induced C28/I2 cells detected by ELISA.

Additional file 2: Supplementary Figure 1. Forest plots of ACE I/D polymorphism associated with susceptibility to prostate cancer based on HWE. ACE I/D: angiotensin-converting enzyme insertion/deletion. A: Model of allelic gene; B: Model of recessive gene inheritance; C: Model of dominant gene inheritance; D: Model of heterozygous gene inheritance; E: Model of homozygous gene inheritance. HWE: Hardy-Weinberg equilibrium.

Additional file 7: Fig. S7. Only a few proteins and transcripts have potential impacts on TMA production. A Ranked abundance plot of proteins and transcripts in duck livers. The top 10 most abundant proteins accounted for 12.7% of the total proteins, and the top 10 most abundant transcripts covered 33.8% of all transcripts in this tissue. B Changes in protein and transcript abundances. The upper-right panel shows the proportion of significantly altered proteins and transcripts.

Additional file 9: Fig. S9 Mutational profilesof CSF and plasma samples at baseline and after 8 weeks of treatment.

Supplemental material, sj-tif-2-cll-10.1177_09636897221139103 for Low-Dose Anti-Thymocyte Globulin Plus Low-Dose Posttransplant Cyclophosphamide as an Effective Regimen for Prophylaxis of Graft Versus Host Disease After Haploidentical Peripheral Blood Stem Cell Transplantation With Maternal/Collateral Related Donors by Ting Li, Qiaomei He, Jun Yang, Yu Cai, Chongmei Huang, Xiaowei Xu, Huiying Qiu, Jiahua Niu, Kun Zhou, Yin Zhang, Xinxin Xia, Yu Wei, Chang Shen, Xueying Ding, Yin Tong, Liping Wan and Xianmin Song in Cell Transplantation

BETA PRIME: Phase I study of AdAPT-001 as monotherapy and combined with a checkpoint inhibitor in superficially accessible, treatment-refractory solid tumors Infographic

Additional file 10: Supplementary Figure S10. Spearman correlation coefficient between 22 immune cells was calculated for CESC (A), OV (B), UCEC (C), and UCS D. Red bubbles indicate positive correlations and blue bubbles indicate negative correlations. The numbers in the bubbles represent the correlation coefficients.