Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

The Wandashan accretionary complex (AC) is located in the easternmost part of Northeast China and consists of the Raohe complex in the east and Yuejinshan complex in the west. It represents an excellent location for insights into Paleo-Pacific subduction and accretion processes. However, the protolith (mainly contains Ocean Plate Stratigraphy ‘OPS’ and ophiolite) nature and tectonic evolution of the Wandashan AC are under debate. This contribution reports three representative geological sections, conducts the OPS reconstruction,...

The Wandashan accretionary complex (AC) is located in the easternmost part of Northeast China and consists of the Raohe complex in the east and Yuejinshan complex in the west. It represents an excellent location for insights into Paleo-Pacific subduction and accretion processes. However, the protolith (mainly contains Ocean Plate Stratigraphy ‘OPS’ and ophiolite) nature and tectonic evolution of the Wandashan AC are under debate. This contribution reports three representative geological sections, conducts the OPS reconstruction,...

TableS1. Clinical data of ovarian cancer patients from TCGA and ICGC.
TableS2.Identified DNA driver methylation (DNAme) in TCGA, ICGC and GSE146556.
TableS3. The correlation between DNAme and corresponding mRNA expression in TCGA, ICGC and GSE146556. TableS4.Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of DNAme-associated mRNA in TCGA.
TableS5. Univariate Cox regression analysis of DNAme-associated mRNA. (P < 0.05)
TableS6. Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of...

Additional file 1: Supplemental Figure 1. Determining the optimal cutoff value of LND for predicting cancer-specific survival using x tile software. (A) Bar graph representing population distribution; (B) Kaplan–Meier survival curves divided by LND cutoff; (C) relative risk analysis among subgroups divided by cutoff value of LND.

Additional file 2: Supplementary Fig. S1. TNFAIP2 expression in normal tissues and cancer cell lines. (a) TNFAIP2 expression in normal tissues, analyzed by GTEx portal; (b) TNFAIP2 expression in cancer cell lines analyzed by CCLE.

Additional file 3: Supplementary Fig. S2. Epigenetic alterations of TNFAIP2 in AML. (a) The exact location of CpG sites in gene body of TNFAIP2 from UCSC database. (b) Kaplan-Meier analysis of the prognostic value of TNFAIP2 methylation at gene body region on OS in AML patients, analyzed by MethSurv.

Additional file 3: Fig. S1. A, B PCA based on the six pyroptosis-related genes signature. C, D The relationship between the risk score and metastasis. E-G The nomogram calibration curves for predicting 1-, 3-, and 5-year survival in the GSE21257 cohort. H Kaplan–Meier analysis based on the pan-cancer data set and univariate Cox regression analysis of CHMP4C. I The expressions of CHMP4C in tumor and adjacent normal tissues.

Additional file 4: Fig. S2. The PPI network.

Additional file 5: Supplementary Fig. S4. Prognostic value of hub genes for OS in AML patients.

Additional file 2: Fig S2. Construct of intermediate cloning vectors for preparing Donor DNA. (A) Fragment A cloned from BW25113-T7 was concatenated to pACYCD-Blank to assemble pACYCD-gene. (B) Reverse-PCR to constructed pACYCD-Donor. (C) The map of Donor DNA, which contains HRL and HRR.

Additional file 3: Supplementary figure 3. The expression of TRIM34, SLC30A10, HOXD4 and circ_0007675 in CLL patients and normal B cells.(A) The expression of TRIM34 in patient specimens were significantly increased. (B) The expression of SLC30A10 in patient specimens were significantly increased. (C) The expression of HOXD4 in patient specimens were significantly increased. (D) The expression of circ_0007675 in patient specimens were significantly increased. All results are expressed as mean ± SEM.

Supplementary Material 6

Supplementary figure 1-3 Supplementary table 1-3

Additional file 3: Figure S3. Tissue expression of differentially expressed protein-coding genes in atm mutated flies after ionizing radiation. A Heatmap of differentially expressed genes expressed in larval tissues. B Heatmap of differentially expressed genes expressed in adult female tissues. C Heatmap of differentially expressed genes expressed in adult male tissues.

Additional file 6: Figure S2. Western blotting of recombinant protein of CsFLS2 and CsFLS3.

Additional file 9: Figure S4. Cis-acting elements within the promoters of CsFLS2 and CsFLS3.

Supplementary Material 17

Additional file 1: Fig. S1. Projection of totaL AF/AFL incidence and death and their age-standardized rates from, 1990 TO 2034.

Additional file 3: Figure S1. Analysis of chromosomal location and gene structure of the CsFLS genes in C. sativa.

Supplemental material, sj-tif-5-tej-10.1177_20417314221132093 for A new cell-free therapeutic strategy for liver regeneration: Human placental mesenchymal stem cell-derived extracellular vesicles by Ting Li, Yu Fu, Zeyi Guo, Honglei Zhu, Hangyu Liao, Xiaoge Niu, Lin Zhou, Shunjun Fu, Yang Li, Shao Li, Lujia Wang, Yizhou Zheng, Lei Feng, Yi Gao and Guolin He in Journal of Tissue Engineering

Supplemental material, sj-jpg-2-tej-10.1177_20417314221132093 for A new cell-free therapeutic strategy for liver regeneration: Human placental mesenchymal stem cell-derived extracellular vesicles by Ting Li, Yu Fu, Zeyi Guo, Honglei Zhu, Hangyu Liao, Xiaoge Niu, Lin Zhou, Shunjun Fu, Yang Li, Shao Li, Lujia Wang, Yizhou Zheng, Lei Feng, Yi Gao and Guolin He in Journal of Tissue Engineering

Additional file 1: Supplemental Figure 1. The cytotoxicity of B7-H3CAR-T cells against solid tumor cell lines was analyzed by RTCA assay. The redarrow represents the time point of effector cell addition.

Long non-coding RNA (lncRNA) PMS2L2 has been reported to participate in endotoxin-induced inflammatory responses. As these types of responses can promote osteoarthritis (OA), it was of interest to ascertain if PMS2L2 may be involved in OA. To explore any potential participation of PMS2L2 in OA, synovial fluid was extracted from both OA patients and healthy controls (n = 62 each) and PMS2L2 expression of each sample determined by RT-qPCR. In addition, as miR-34a has a...