58 Works

Additional file 2 of LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1

Yue Li, Chen Chen, Hai-lin Liu, Zhen-fa Zhang & Chang-li Wang
Additional file 2: Figure S1. Depletion of LARRPM promoted proliferation, repressed apoptosis and promoted migration and invasion of LUAD cells. A LARRPM expression in HCC827 cells with LARRPM stable depletion or control was detected by qRT-PCR. B Cell proliferation of HCC827 cells with LARRPM stable depletion or control was detected using CCK-8 assays. C Cell proliferation of HCC827 cells with LARRPM stable overexpression or control was detected using EdU incorporation assays. Scale bar: 100 µm....

Additional file 6 of LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1

Yue Li, Chen Chen, Hai-lin Liu, Zhen-fa Zhang & Chang-li Wang
Additional file 6: Fig. S5. Anti-CSF1 antibody abolished the effects of LARRPM on M2 macrophage infiltration. A THP-1 cells were subjected to transwell migration assays towards A549 cells with LARRPM overexpression or A549 control cells treated with anti-CSF1. Scale bar: 100 µm. B THP-1 cells were subjected to transwell migration assays towards HCC827 cells with LARRPM depletion or HCC827 control treated with anti-CSF1. Scale bar: 100 µm. C M1 and M2 polarization markers expression in...

Additional file 3 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 3: Fig S2. Origin and evolution of LSSL. (A) The phylogenetic tree for the LSSL family based on the neighbor-joining (NJ) method. An NJ tree was constructed using the amino acid sequences of the LSSL proteins. All species represented by abbreviations are shown in Table S2. The bar (0.020) indicates the genetic distance. Different color lines correspond to different species groups. (B) The motif composition of the LSSL family proteins. (C) Model diagram...

Additional file 5 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 5: Fig S4. Glycan selectivity of LSSL assessed by glycan microarrays. (A) LSSL (8 µg/mL) binding to 100 glycan microarray. The concentrations given for the glycan microarray represent those used in the carbohydrate immobilization reaction. Data are presented as the mean ± SDs; (n = 3 technical replicates). (B) Confocal microscopy detection of LSSL binding to yeast. LSSL were incubated with yeast cells (105 cells/well) in PBS for 1 h at room temperature...

Additional file 1 of Identification of DNA methylation-regulated differentially expressed genes in RA by integrated analysis of DNA methylation and RNA-Seq data

Runrun Zhang, Cen Chang, Yehua Jin, LingXia Xu, Ping Jiang, Kai Wei, Linshuai Xu, Shicheng Guo, Songtao Sun & Dongyi He
Additional file 1: Data Quality Control (QC).

Additional file 7 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 7: Fig S6. Elimination of C3 from serum to detect the deposition of LSSL and MASP-1 on the surface of HeLa cells. (A) Quantitative analysis of proteins on the HeLa cells analyzed by Alexa-488 staining followed by flow cytometry. (B, C) The histogram shows the fluorescence intensity and cell proportions of the above flow cytometry results, respectively. The data are presented as the means ± SDs. (D) Western blotting analysis of depleted and...

Additional file 9 of Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Xuewen Zhu, Yaolei Mi, Xiangxiao Meng, Yiming Zhang, Weiqiang Chen, Xue Cao, Huihua Wan, Wei Yang, Jun Li, Sifan Wang, Zhichao Xu, Atia Tul Wahab, Shilin Chen & Wei Sun
Additional file 9: Figure S4. Cis-acting elements within the promoters of CsFLS2 and CsFLS3.

Additional file 1 of Identification of microbial taxa present in Ctenocephalides felis (cat flea) reveals widespread co-infection and associations with vector phylogeny

Charlotte Manvell, Hanna Berman, Benjamin Callahan, Edward Breitschwerdt, William Swain, Kelli Ferris, Ricardo Maggi & Erin Lashnits
Additional file 1: Figure S1. Decontam assigned metric score for ASVs from the genera identified to have at least one ASV with a decontam score above the minimum from Bartonella, Rickettsia and Wolbachia (> 0.89). Those identified as candidate microbiome members on the basis of literature search are outlined in green while the known microbiome members are outlined in red.

Additional file 1 of Identification of microbial taxa present in Ctenocephalides felis (cat flea) reveals widespread co-infection and associations with vector phylogeny

Charlotte Manvell, Hanna Berman, Benjamin Callahan, Edward Breitschwerdt, William Swain, Kelli Ferris, Ricardo Maggi & Erin Lashnits
Additional file 1: Figure S1. Decontam assigned metric score for ASVs from the genera identified to have at least one ASV with a decontam score above the minimum from Bartonella, Rickettsia and Wolbachia (> 0.89). Those identified as candidate microbiome members on the basis of literature search are outlined in green while the known microbiome members are outlined in red.

Additional file 1 of Identification of DNA methylation-regulated differentially expressed genes in RA by integrated analysis of DNA methylation and RNA-Seq data

Runrun Zhang, Cen Chang, Yehua Jin, LingXia Xu, Ping Jiang, Kai Wei, Linshuai Xu, Shicheng Guo, Songtao Sun & Dongyi He
Additional file 1: Data Quality Control (QC).

Additional file 3 of Identification of DNA methylation-regulated differentially expressed genes in RA by integrated analysis of DNA methylation and RNA-Seq data

Runrun Zhang, Cen Chang, Yehua Jin, LingXia Xu, Ping Jiang, Kai Wei, Linshuai Xu, Shicheng Guo, Songtao Sun & Dongyi He
Additional file 3: Correlation between mRNA expression and methylation level of other genes.

Additional file 3 of Rapid and simultaneous detection of multiple pathogens in the lower reproductive tract during pregnancy based on loop-mediated isothermal amplification-microfluidic chip

Xiaofang Xu, Yiguo Jia, Ruolin Li, Yuting Wen, Yuchen Liang, Guangjie Lao, Xiaojuan Liu, Wei Zhou, Huawei Liu, Jiang Xie, Xiaoxia Wang, Wenming Xu & Qun Sun
Additional file 3. Original gel of PCR products in the validation experiments of clinical samples with positive chip results for G. vaginalis.

Additional file 3 of Rapid and simultaneous detection of multiple pathogens in the lower reproductive tract during pregnancy based on loop-mediated isothermal amplification-microfluidic chip

Xiaofang Xu, Yiguo Jia, Ruolin Li, Yuting Wen, Yuchen Liang, Guangjie Lao, Xiaojuan Liu, Wei Zhou, Huawei Liu, Jiang Xie, Xiaoxia Wang, Wenming Xu & Qun Sun
Additional file 3. Original gel of PCR products in the validation experiments of clinical samples with positive chip results for G. vaginalis.

Additional file 3 of LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1

Yue Li, Chen Chen, Hai-lin Liu, Zhen-fa Zhang & Chang-li Wang
Additional file 3: Figure S2. The correlation between LARRPM expression, 5hmC level at LINC00240 promoter, CpG43 methylation level and LINC00240 expression in LUAD tissues. A Correlation between LINC00240 and LARRPM expression analysed using the TCGA LUAD data. r = 0.6164, P < 0.0001 by Spearman correlation analysis. B Correlation between LINC00240 and LARRPM expression analyzed in our LUAD cohort. r = 0.5521, P < 0.0001 by Spearman correlation analysis. C 5hmC levels of CpG43 from...

Additional file 5 of LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1

Yue Li, Chen Chen, Hai-lin Liu, Zhen-fa Zhang & Chang-li Wang
Additional file 5: Figure S4. The correlation between LARRPM expression, 5hmC level at CSF1 promoter, CpG82 methylation level and CSF1 expression in LUAD tissues. A Correlation between CSF1 and LARRPM expression analysed using the TCGA LUAD data. r = − 0.3069, P < 0.0001 by Spearman correlation analysis. B Correlation between CSF1 and LARRPM expression analysed in our LUAD cohort. r = − 0.5774, P < 0.0001 by Spearman correlation analysis. C 5hmC levels of...

Additional file 7 of Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Xuewen Zhu, Yaolei Mi, Xiangxiao Meng, Yiming Zhang, Weiqiang Chen, Xue Cao, Huihua Wan, Wei Yang, Jun Li, Sifan Wang, Zhichao Xu, Atia Tul Wahab, Shilin Chen & Wei Sun
Additional file 7: Figure S3. Comparsion of CsFLS2 and CsFLS3 with other proteins belonging to DOXC 28/47 subgroup of 2-ODD superfamily.

Additional file 7 of Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Xuewen Zhu, Yaolei Mi, Xiangxiao Meng, Yiming Zhang, Weiqiang Chen, Xue Cao, Huihua Wan, Wei Yang, Jun Li, Sifan Wang, Zhichao Xu, Atia Tul Wahab, Shilin Chen & Wei Sun
Additional file 7: Figure S3. Comparsion of CsFLS2 and CsFLS3 with other proteins belonging to DOXC 28/47 subgroup of 2-ODD superfamily.

Suppl FigS1_Breeding scheme to generate KO

Carole Wilson, Chi F. Hung, Brian Burkel, Suzanne M. Ponik, Sina Gharib & Lynn M. Schnapp
Generation of mice with the nephronectin null allele using a Pdgfrb-Cre transgenic

Additional file 2 of SWI/SNF complex gene variations are associated with a higher tumor mutational burden and a better response to immune checkpoint inhibitor treatment: a pan-cancer analysis of next-generation sequencing data corresponding to 4591 cases

Yue Li, Xinhua Yang, Weijie Zhu, Yuxia Xu, Jiangjun Ma, Caiyun He & Fang Wang
Additional file 2: Fig. S2 The progression-free survival (PFS) of patients receiving immune checkpoint inhibitor (ICI) treatment based on cancer types. The survival analysis was performed for individual cancer types that contained at least 10 cases in the SWI/SNF-mutant or SWI/SNF-non-mutant groups. The PFS of the SWI/SNF-mutant group was significantly superior to that of the SWI/SNF-non-mutant group in colorectal cancer (a) and gastric cancer (b), the same tendency was significant numerically by not statistically in...

Additional file 4 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 4: Fig S3. Cryo-EM analysis. (A) A flow-chart of cryo-EM data processing. (B) Subunit structure in LSSL trimer. (C) Fold diagram for the structure of LSSL. (D) FSC curve (gold standard FSC) as a function of before and after mask application.

Suppl FigS3_Transmission electron microscopy of embryonic lungs

Carole Wilson, Chi F. Hung, Brian Burkel, Suzanne M. Ponik, Sina Gharib & Lynn M. Schnapp
Analysis of the visceral pleura in a WT and nephronectin KO lung at E16.5 by transmission electron microscopy

Additional file 8 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 8: Fig S7. Elimination of LSSL from serum to detect the deposition of VLRB and C1q on the surface of HeLa cells. (A) Immuno-fluorescence detection of VLRB and C1q deposited on the surface of HeLa cells treated with lamprey serum and LSSL-depleted serum. Scale bars, 10 µm. (B) A histogram shows the statistics of the above-mentioned results. The data are presented as the means ± SDs. (C) Western blotting analysis with mouse anti-VLRB...

Additional file 6 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 6: Fig S5. Elimination of MASP-1 from serum to detect the deposition of LSSL and C3 on the surface of Hela cells. (A) Quantitative analysis of proteins on HeLa cell membranes analyzed by Alexa-488 staining followed by flow cytometry. (B, C) The histogram shows the fluorescence intensity and cell proportions of the above flow cytometry results, respectively. The data are presented as the means ± SDs. (D) Western blotting analysis of depleted and...

Additional file 4 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 4: Fig S3. Cryo-EM analysis. (A) A flow-chart of cryo-EM data processing. (B) Subunit structure in LSSL trimer. (C) Fold diagram for the structure of LSSL. (D) FSC curve (gold standard FSC) as a function of before and after mask application.

Additional file 6 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 6: Fig S5. Elimination of MASP-1 from serum to detect the deposition of LSSL and C3 on the surface of Hela cells. (A) Quantitative analysis of proteins on HeLa cell membranes analyzed by Alexa-488 staining followed by flow cytometry. (B, C) The histogram shows the fluorescence intensity and cell proportions of the above flow cytometry results, respectively. The data are presented as the means ± SDs. (D) Western blotting analysis of depleted and...

Registration Year

  • 2022
    58

Resource Types

  • Image
    58

Affiliations

  • University of Wisconsin–Madison
    58
  • Sun Yat-sen University
    42
  • Second Hospital of Anhui Medical University
    42
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    38
  • Yangzhou University
    36
  • Anhui Medical University
    36
  • Zhejiang University
    34
  • Sichuan University
    28
  • Second Military Medical University
    26
  • First Affiliated Hospital of Zhengzhou University
    26