Additional file 4. Tucidinostat plus checkpoint blockade induces significant antitumor immunity. a Schema of the experiment. Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). The proportion of intratumoral CD8+ T cells...

Additional file 4. Tucidinostat plus checkpoint blockade induces significant antitumor immunity. a Schema of the experiment. Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). The proportion of intratumoral CD8+ T cells...

Additional file 5. Tucidinostat plus checkpoint blockade induces CCL5 secretion. a Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with different doses (12.5, 25, 75 mg/kg, gavage, daily, n=5) of tucidinostat. The expression of CCL5 in peripheral blood serum from CT26 tumor-bearing mice on day 10 post treatment initiation. Mouse CT26 cells (5 ×...

Additional file 8: Figure S1. PPI network based on the analysis of 134 overlapping genes using Cytoscape.

Additional file 5: Figure S5. Percentage of age-standardised DALYs due to early-onset colorectal cancer attributable to risk factors for 21 GBD regions, both sexes, 2019. GBD: Global Burden of Diseases, Injuries, and Risk Factors Study; SDI: Socio-demographic index; DALYs, Disability-Adjusted Life Years.

Additional file 1: Figure S1. Distribution of the proportion of surveys being infected in Kombewa (a), Iguhu (b), and Marani (c) in western Kenya.

Additional file 6: Figure S3. Variations in monthly maximum temperature, minimum temperature, mean temperature and monthly rainfalls in Kombewa (a), Iguhu (b), and Marani (c) in western Kenya.

Additional file 1: Fig. S1. Projection of totaL AF/AFL incidence and death and their age-standardized rates from, 1990 TO 2034.

Additional file 4: Fig. S4. In 2019, the total rosk factors in 5 SDI regions were attributed to age-standardized death and Dalys in AF/AFL.

Additional file 4: Fig. S4. In 2019, the total rosk factors in 5 SDI regions were attributed to age-standardized death and Dalys in AF/AFL.

LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

Additional file 1. Supplementary Fig 1. TRIM46 reduces TBK1 expression and interacts with TBK1 in HEK293T cells. (A) HEK293T cells were transfected with 1μg TBK1-Flag plasmids with 0 μg (-), 1μg (+) and 3 μg (++) TRIM46-Myc plasmids for 24 h, cells were lysed and subjected to western blotting to detect the expression of Flag tag and Myc tag, GAPDH was used as an internal control. (B) HEK293T cells were transfected with TRIM46-Myc plasmids with...

Efficacy and safety of nivolumab for advanced/recurrent non-small-cell lung cancer: an up-to-date meta-analysis of large-scale phase III randomized controlled trials Supplementary figure 1

Additional file 2: Supplementary figure 2. The relationship between immune-related function and prognosis of CLL patients.

Additional file 2: Figure S2. Age-standardized prevalence rate at the global, regional, and national level. (A) Age-standardized prevalence rate of early-onset colorectal cancer globally and for 21 GBD regions by SDI, 1990–2019. (B) Age-standardized prevalence rates of early-onset colorectal cancer for 204 countries and territories in 2019. The black line represents the expected age-standardized prevalence rate of rheumatic heart disease based solely on SDI. GBD: Global Burden of Diseases, Injuries, and Risk Factors Study; SDI:...

Additional file 3: Figure S3. Age-standardized mortality rate at the global, regional, and national level. (A) Age-standardized mortality rate of early-onset colorectal cancer globally and for 21 GBD regions by SDI, 1990–2019. (B) Age-standardized mortality rates of early-onset colorectal cancer for 204 countries and territories in 2019. The black line represents the expected age-standardized mortality rate of rheumatic heart disease based solely on SDI. GBD: Global Burden of Diseases, Injuries, and Risk Factors Study; SDI:...

Supplemental material, sj-jpg-5-ijs-10.1177_10668969221125793 for Characterization of Intercalated Cell Markers KIT and LINC01187 in Chromophobe Renal Cell Carcinoma and Other Renal Neoplasms by Rahul Mannan, Xiaoming Wang, Pushpinder S. Bawa, Yuping Zhang, Stephanie L. Skala, Anya K. Chinnaiyan, Aniket Dagar, Lisha Wang, Sylvia B. Zelenka-Wang, Lisa M. McMurry, Nikita Daniel, Xuhong Cao, Ankur R. Sangoi, Sounak Gupta, Ulka N. Vaishampayan, Khaled S. Hafez, Todd M. Morgan, Daniel E. Spratt, Maria S. Tretiakova, Pedram Argani, Arul M....

Supplementary Material 7

Additional File 5.

Additional file 1: Figure S1. Identification and validation of prognostic DEMs. (A-B) Lasson analysis evaluated the optimal variables of the multivariate Cox regression analysis; (C)TCGA_BRCA database indicated that the expression of hsa-miR-193a and hsa-miR-449a was inconsistent with the univariate results; (D-F)TCGA_BRCA database verified the expression and prognosis of DEMs including hsa-let-7b, hsa-miR-195 and hsa-miR-301b with the P <0.05. The asterisk (*)means p-value < 0.05.

Additional file 3: Fig. S2. Multicollinearity analysis for BMI and the MNA-SF.

Additional file 5: Fig. S5. Mapchart shown the distribution of GRAS genes on 8 chromosomes in peach.

Additional file 6: Figure sup 6. The key molecules that regulate microglialfunctions are up-regulated in an apoE isoform-dependent manner uponcuprizone-induced demyelination. RNA was extracted from the CC area of CTL(n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice. (A-H) The expression of genes relatedto microglial activation, inflammation, and lipid sensing (Trem2, Tyrobp,Aim2, Btk, B2m, Ctsd, C3, and Cr3) was analyzed by real-time PCR. (I-L) The expression of genes involvedin microglial migration (Mmp2 and Mmp12) and phagocytosis (Axl)were examined...

Additional file 1: Figure sup 1. Opposing effects of apoE2 and apoE4 on Iba1 proteinlevels and their correlation with the amount of myelin debris in the corpuscallosum of apoE-TR mice upon cuprizone-induced demyelination. ApoE-TR micewere fed with either normal diet (CTL, n=5/genotype), or CPZ-containing diet(CPZ, n=5-6/genotype) for four weeks. (A,B) Iba1 protein level inthe CC region was assessed by Western blot (WB) analysis, and the fold change (CPZ vs. CTL) wasquantified. (C) A negative correlation...

Additional File 5.