The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...

Additional file 10: Figure S3. The co-expression levels of S. mansoni proteins identified.

To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Triggering receptor expressed on myeloid cells 1 (TREM1) participates in the development of endometritis. This study aims at identifying the effects and interaction of TREM1 and upstream stimulatory factor 2 (USF2) in endometritis by using a model of lipopolysaccharide (LPS)-induced human endometrial epithelial cells (HEnEpCs). ELISA was performed to determine the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF-α) after LPS stimulation. TREM1 and USF2 expression was examined with RT-qPCR and Western blot....

Long non-coding RNA (lncRNA) PMS2L2 has been reported to participate in endotoxin-induced inflammatory responses. As these types of responses can promote osteoarthritis (OA), it was of interest to ascertain if PMS2L2 may be involved in OA. To explore any potential participation of PMS2L2 in OA, synovial fluid was extracted from both OA patients and healthy controls (n = 62 each) and PMS2L2 expression of each sample determined by RT-qPCR. In addition, as miR-34a has a...

Additional file 2: Figure S2. Results of western blot analysis showed that ETV2 overexpression induced the the activation of p-ERK and p-AKT at days 3, 7 and 14 of osteogenic differentiation. *P < 0.05, compared with the 0 day group.

Additional file 3: Figure S3. HE (scale bar = 200 μm) and Masson (scale bar = 50 μm) staining in the rat calvarial defect model (n = 6).

Additional file 1: Figure S1. Panx 2 and Panx 3 mRNA expression after CCI. A-B. A qPCR assay was used to detect Panx 2 (A) and Panx 3 (B) mRNA levels at 4 and 14 days post-CCI. The data are mean ± SEM. n = 3 mice/group. **p < 0.01, ***p < 0.001, vs. sham injury. The data were analyzed by one-way ANOVA followed by Dunnett’s test.

Additional file 2: Figure S2. The distribution of Panx 1 in the DRG and within sciatic nerves. A. Double-immunofluorescence images of Panx 1 (green) and the sciatic nerve axonal marker, NF200 (red). (B). Images of Panx 1 immunostaining (red) and, Nissl staining (green) in the DRG; Scale bar = 50 μm. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 100 μm. Lower panel, scale bar...

Additional file 3: Figure S3. Domain-based classification of T. aestivum RING-zf proteins. a Additional TM domain contained in the RING-zf protein. b Additional IBR domain contained in the RING-zf protein. c Additional coiled coil domain contained in the RING-zf protein. d Additional RPT1 domain contained in the RING-zf protein. e Additional RING-ZnF-CHY, RING-Zinc_ribbon_6 and pfam Lon-substr-bdg domains contained in the RING-zf protein. f Additional DEXDc, HIRAN and HELICc domains contained in the RING-zf protein. g...

TableS1. Clinical data of ovarian cancer patients from TCGA and ICGC.
TableS2.Identified DNA driver methylation (DNAme) in TCGA, ICGC and GSE146556.
TableS3. The correlation between DNAme and corresponding mRNA expression in TCGA, ICGC and GSE146556. TableS4.Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of DNAme-associated mRNA in TCGA.
TableS5. Univariate Cox regression analysis of DNAme-associated mRNA. (P < 0.05)
TableS6. Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of...

Additional file 1: Supplementary Figure S1. BUSCO assembly evaluation results. C (complete): matches the BUSCO database sequence; F (fragmented): only part of the sequence can be compared with the BUSCO database; D (duplicate): multiple genes are compared with the same BUSCO; M (missing): the filtered sequence.

Additional file 2.

Additional file 3.

Additional file 3: Fig. S2. ADM was upregulated in glioma tissues based on online datasets and clinical collected samples. (A) GSE4412; (B) GSE4290; (C) overall survival of ADM according to CGGA dataset; (D) overall survival of ADM according to TCGA-GBMLGG dataset; (E and F) The mRNA and protein levels of ADM in glioma samples and non-cancerous peritumoral brain edema tissue.

Additional file 3: Figure S3. Domain-based classification of T. aestivum RING-zf proteins. a Additional TM domain contained in the RING-zf protein. b Additional IBR domain contained in the RING-zf protein. c Additional coiled coil domain contained in the RING-zf protein. d Additional RPT1 domain contained in the RING-zf protein. e Additional RING-ZnF-CHY, RING-Zinc_ribbon_6 and pfam Lon-substr-bdg domains contained in the RING-zf protein. f Additional DEXDc, HIRAN and HELICc domains contained in the RING-zf protein. g...

Additional file 6: Fig. S3. Different from TTC12 expression that restricted to spermatocytes and respiratory ciliated cells (upper), TTC21B was universally expressed in most of tissues but possess relative lower expression levels in spermatocytes and respiratory ciliated cells (bottom) according to Human Protein Atlas Database ( www.proteinatlas.org )

Additional file 3: Fig. S2. Exome-based copy number alteration detection was performed followed by MLPA confirmation. (A-B) CNV analysis identified 16p13.11 microduplication (chr16: 14.82-15.12, 300kb) in the F1-II-1 (607P) but not in F1-II-2 (607S). The individual 692F served as a control. (C) MLPA confirmation of the gain of copy in PDXDC1, located in the 16p13.11 region, that identified from CNV analysis in the 607-P when compared with 607-S. Three independent probes were designed for each...

Additional file 4: Figures S3. KEGG pathway enrichment analysis of the 12 differentially expressed proteins.

Additional file 3: Figures S2. GO enrichment analysis of the 12 differentially expressed proteins.

Additional file 1: Fig. S1. DNA methylation level trends. (A) Bean plots showing the methylation level distribution of methylation sites in the context of CpG, CHG and CHH at genome wide scale. (B) The methylation level trends around gene features per sample. a = promoter (2 kb upstream of transcription start site); b = first exon; c = first intron; d = inner exons; e = inner introns; f = last exon; g = downstream...

Additional file 2: Fig. S2. Methylation status of differentially methylated sites. (A-B) Volcano plots showing DMCs in the context of CHG (A) and CHH (B). (C) Bar plots showing distribution of differences in methylation levels of DMCs.

Additional file 4: Fig. S4. DNA methylation landscape. (A) The number of DMCs grouped by the differences in methylation levels per chromosome. (B-D) Manhattan plots showing the global distribution of DMCs in the context of CpG (B), CHG (C) and CHH (D). (E) DNA methylation landscape of chromosome 2.

Additional file 5: Fig. S5. Effect of cilostazol treatment on cardiac function after CLP. a Representative M-mode images of the four different groups. b-e Quantitative analysis of LVEF (b), LVFS (c), LVEDD (d), and LVESD (e) in each group (n = 6). These data indicate no significant difference.

Additional file 6: Fig. S6. Single-cell sequencing analysis of adult mice intestine. a Dotplot for highest specificity gene markers of cell clusters. Dot size represents the percentage of cells expressing the denoted gene, and the color represents average normalized expression level within the denoted cluster. b The expression of GFAP in different cell clusters by analysis of the Uniform Manifold Approximation and Projection (UMAP). c Different cell subpopulation of the GSE156905 dataset derived from the...