Additional file 2: Supplementary figure 2. The relationship between immune-related function and prognosis of CLL patients.

Additional file 1: Supplementary Figure 1. The original and uncropped gel image of AAT1.

Supplementary Material 2

Supplementary Material 2

Supplementary Material 2

Additional file 6: Fig. S3. Different from TTC12 expression that restricted to spermatocytes and respiratory ciliated cells (upper), TTC21B was universally expressed in most of tissues but possess relative lower expression levels in spermatocytes and respiratory ciliated cells (bottom) according to Human Protein Atlas Database ( www.proteinatlas.org )

Additional file 3: Fig. S2. Exome-based copy number alteration detection was performed followed by MLPA confirmation. (A-B) CNV analysis identified 16p13.11 microduplication (chr16: 14.82-15.12, 300kb) in the F1-II-1 (607P) but not in F1-II-2 (607S). The individual 692F served as a control. (C) MLPA confirmation of the gain of copy in PDXDC1, located in the 16p13.11 region, that identified from CNV analysis in the 607-P when compared with 607-S. Three independent probes were designed for each...

Additional file 2: Fig. S2. Methylation status of differentially methylated sites. (A-B) Volcano plots showing DMCs in the context of CHG (A) and CHH (B). (C) Bar plots showing distribution of differences in methylation levels of DMCs.

Additional file 3: Fig. S2. Multicollinearity analysis for BMI and the MNA-SF.

The alkaloids of Narcissus tazetta L. var. Chinensis Roem (Amaryllidaceae) have antitumor and antiviral activities. However, the immunopharmacological effects of one of its constituents, pseudolycorine chloride (PLY), have not been reported yet. We evaluated the effect of PLY on myeloid-derived suppressor cells (MDSCs) expansion and differentiation into monocyte-like MDSCs (M-MDSCs) and examined whether PLY alleviates Th17 cell-mediated experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS). In vitro, MDSCs were treated with PLY...

Additional file 1: Figure S1. Pie chart shows the number of differentially expressed lncRNAs in each category.

Additional file 4: Figure S3. Volcano plot showing the log2 fold change of 517 significantly differentially expressed miRNAs in CRC patients from the TCGA database.

Additional file 8: Figure S1. PPI network based on the analysis of 134 overlapping genes using Cytoscape.

Additional file 4: Figure S1. Phylogenetic analysis of Pvs230 sequences from global P. vivax populations. The maximum-likelihood phylogenetic tree reconstructed based on alignment by ClustalW with bootstrap analysis to assess clade support (500 replicates) was shown for Pvs230 global isolates. The global isolates were clustered into three main groups, the branches of which are shown in red (group 1), green (group 2), and blue (group 3).

Additional file 2: Figure S2. Phylogenetic relationships, gene structure and architecture of conserved protein motifs in 138 TaRING-zfs from T. aestivum. a The name of 138 TaRING-zf genes. b Exon, intron, and UTR structure of 138 TaRING-zf genes. Yellow boxes indicate untranslated 5’ and 3’ regions, green boxes indicate exon, and black lines indicate introns. The gene full length and protein length can be estimated by using the scale at the bottom. c The motif...

Additional file 6: Supplementary Figure 6.

Additional file 1: Figure S1. Phenotype of wild-type (WT), cef3 and cesa9 plants at seedling stage. The seedling of cesa9 is easily broken, while seedlings of WT and cef3 are normal after hand flexing

Additional file 2: Figure S2. CEF3 amino acid sequence alignment of WT and cef3 mutant

Additional file 4: Figure S4. Deduced CEF3 amino acid sequence alignments for the three homozygous mutants generated by CRISPR-Cas9 system and WT.

Additional file 6: Figure S6. Phenotypic comparison among the wild-type and cef3 plants generated by CRISPR–Cas9 system. (A) The phenotype of the wild-type (WT) and cef3 mutants generated by CRISPR-Cas9 system at the mature stage. Scale bars: 10 cm. (B) Folding the internodes of the wild-type, cef3-c2 and cef3-c3. Scale bars: 2 cm (C) The plant height of the wild-type, cef3-c2 and cef3-c3. (D) The extension force of culms in the wide-type, cef3-c2 and cef3-c3...

Additional file 7: Figure S7. (A) Phylogenetic tree of CEF3 in rice and Arabidopsis. (B) The amino acid sequence alignment between the AtSCD2 and OsCEF3. Different rectangles represent different functional domains.

Additional file 4: Figure S4. TPM1 knockdown exacerbates inflammation in the TREM2−/− mouse retina. A, B Western blot analysis (A) and quantification of TPM1 (B) in the retina of C57BL/6J (WT) and TREM2−/− mice. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test (WT vs. TREM2−/−, **p < 0.01). C, D Western blot analysis (C) and quantification of TREM2 (D) in WT mice following LPS and siCTR or siTPM1-1...

Additional file 5: Figure S5. TPM1 knockdown changes transcriptome associated with glial cells in WT mouse retina. A A heatmap showing DEGs associated with microglia and astrocytes in WT mice following treatments with PBS, or with LPS and siTPM1-1 or siCTR. B–G The expression levels of DEGs associated with M1 (B) or M2 microglia (C, D) and with A1 (E) or A2 astrocytes (F, G) in WT mice after treatment with PBS, or with LPS...

Additional file 4. Tucidinostat plus checkpoint blockade induces significant antitumor immunity. a Schema of the experiment. Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). The proportion of intratumoral CD8+ T cells...

Additional file 4. Tucidinostat plus checkpoint blockade induces significant antitumor immunity. a Schema of the experiment. Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). The proportion of intratumoral CD8+ T cells...