Additional file 6: Figure S1. The representative MS/MS spectrum of LTGMAFR, supporting both B. glabrata and S. mansoni glyceraldehyde-3-phosphate dehydrogenase (G3PDH).

Additional file 3.

Long non-coding RNAs (lncRNAs) are important players in regulating diverse human diseases, including cancers. Nonetheless, the function of long intergenic non-protein coding RNA 662 (LINC00662) in gastric cancer (GC) carcinogenesis and progression remains to be delineated. In the present study, LINC00662, microRNA-195-5p (miR-195-5p) and centrosomal protein 55 (CEP55) mRNA expression levels were quantified by qRT-PCR. GC cell proliferation, migration and invasion were analyzed by CCK-8, BrdU and Transwell assays. Besides, dual-luciferase reporter and RNA pull-down...

Numerous studies have reported the regulatory effects of miR-21-5p and reversine in human breast cancer (HBC). However, the mechanism of reversine and miR-21-5p has not been fully investigated in HBC. The aim of the current study was to assess the mechanism of action of reversine, with or without miR-21-5p, in HBC progression. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot results confirmed the upregulation of miR-21-5p and downregulation of sprouty RTK signaling antagonist 2...

Additional file 2: Figure. S1. Unrooted phylogenetic tree of NlSPARC from N.lugens and representative insect species. An unrooted phylogenetic tree was constructed by the neighbour-joining tree construction program Mega 7. Evolutionary distances were computed using Poisson correction method. Branch support values (1000 bootstraps) for nodes are indicated only support values > 50% are shown. NlSPARC is marked with filled triangle. All protein sequences (accession numbers, length and pI) obtained from GenBank was listed in Table...

Additional file 1: Figure S1. The mRNA level of CBFA1 in qRT-PCR (a) and RNA-Seq (b). *P < 0.05.

Additional file 4: Figure S4. HE (scale bar = 50 μm) and Masson (scale bar = 50 μm) staining were performed to evaluate ectopic bone formation in nude mice (n=4).

SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Additional file 2: Fig. S2 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of maternal parent (P2).

Additional file 1: Supplementary Figure 1. Illustrations for the length of bowel resection, location of related arteries ligation and scope of the lymph node dissection for radical left colectomy for tumours located (A) at the distal 1/3 of the transverse colon (TC) and splenic flexure (SF), (B) at the upper segment of descending colon (UDC), (C) at the lower segment of descending colon (LDC).

Additional file 4: Fig. S2. The PPI network.

Additional file 1: Figure S1. (A) WT-HeLa cells were treated with 20 μM of J3 for 3, 6, and 12 h for western blot, quantification of the protein expression of p62 (B) and LC3 (C) was analyzed. (D) GFP-LC3-HeLa were treated as (A), GFP-LC3 dots were analyzed. Scale bar = 20 μm. (E) GFP-LC3 dots of (D) were quantified. (F) GFP-LC3-MEF and GFP-LC3-A549 cells were incubated with 20 μM of J3 for 6 h. GFP-LC3...

Additional file 2: Figure S2. WT-HeLa cells were incubated with 20 μM of J3 with or without 2 mM of NAC, 1 μM of compound C, 10 μM of U0126, 10 μM of BAPTA for 6 h, respectively. The protein level of LC3-II was detected by western blot and quantified.

Additional file 5: Figure S5. (A) The flow diagram of the mice generation, genotype identification, and the administration methods (HdhQ140/Q140 and WT mice are not littermates in our study). (B) Dissection of the mice striatum were shown in the picture, the red line indicates the outline for a piece of striatum. (C) The immunostaining of T-HTT (anti-HTT, MAB2166) in the striatum of WT and homozygous mice was analyzed at 12-month-old. (D) The expression of total...

Additional file 5: Figure S5. (A) The flow diagram of the mice generation, genotype identification, and the administration methods (HdhQ140/Q140 and WT mice are not littermates in our study). (B) Dissection of the mice striatum were shown in the picture, the red line indicates the outline for a piece of striatum. (C) The immunostaining of T-HTT (anti-HTT, MAB2166) in the striatum of WT and homozygous mice was analyzed at 12-month-old. (D) The expression of total...

Additional file 6: Figure S6. (A-B) Analysis of the expression level of T-HTT (anti-HTT, MAB2166) and DARPP32 in the striatum of 15-month-old homozygous HdhQ140/Q140 mice with or without 60 mg/kg of J3 administration for 3 months. Data are presented as mean ± sem from three individual samples. *p < 0.05, **p < 0.01, ***p < 0.001. (C) Values of parameters characterizing physicochemical and basic ADME properties calculated using ACD/Percepta.

Additional file 4: Figure S4. The correlation of pyroptosis-related prognostic genes and clinical features in the training set. (A, B, C) The correlation of NLRP1 with grade, N stage, T stage. (D) The correlation of NLRP2 with grade. (E) The correlation of GSDMC with stage. (F) The correlation of CASP4 with grade. (G) The correlation of risk score with grade.

Additional file 5: Figure S5. GSEA enrichment analysis identifies KEGG pathways associated with high-risk and low-risk groups in the training set (A) and test set (B).

Additional file 9: Figure S9. Overexpression GSDMC promotes cell proliferation and invasion in PAAD. (A)PANC-1 (left panel) and CFPAC-1 (right panel) cells were subjected to immunoblotting as indicated. (B, C) Cell growth of PANC-1 cells (B) and CFPAC-1 cells (C) were measured by CCK-8 assay. *P < 0.05, **P < 0.01 by two-way ANOVA. (D, F) Clone formation of PANC-1 cells (D) and CFPAC-1 cells (F) were measured as indicated ***P < 0.001 by a...

Supplementary Material 3

Supplementary Material 4

Supplementary Material 5

Supplementary Material 7

Supplementary Material 10

Additional file 3: Figure S3. Significantly regulated PI3K pathway genes in HeLa cells 2 h after 4 Gy irradiation compared to unirradiated cells