989 Works

Circular RNA circCHFR downregulation protects against oxidized low-density lipoprotein-induced endothelial injury via regulation of microRNA-15b-5p/growth arrest and DNA damage inducible gamma

Yang Li & Bing Wang
Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

High hydrostatic pressure (30 atm) enhances the apoptosis and inhibits the proteoglycan synthesis and extracellular matrix level of human nucleus pulposus cells via promoting the Wnt/β-catenin pathway

Zongting Shi, Jun He, Jian He & Yuan Xu
Hydrostatic pressure is known to regulate bovine nucleus pulposus cell metabolism, but its mechanism in human nucleus pulposus cells (HNPCs) remains obscure, which attracts our attention and becomes the focus in this study. Specifically, HNPCs were treated with SKL2001 (an agonist in the Wnt/β-catenin pathway) or XAV-939 (an inhibitor of the Wnt/β-catenin pathway), and pressurized under the hydrostatic pressure of 1, 3 and 30 atm. The viability, apoptosis and proteoglycan synthesis of treated HNPC were...

Melatonin affects hypoxia-inducible factor 1α and ameliorates delayed brain injury following subarachnoid hemorrhage via H19/miR-675/HIF1A/TLR4

Zhijian Xu, Fengduo Zhang, Hu Xu, Fan Yang, Gezhi Zhou, Minfeng Tong, Yaqiang Li & Song Yang
This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Hyperthermia inhibits growth of nasopharyngeal carcinoma through degradation of c-Myc

Xiaole Li, Shichao Duan, Yingjuan Zheng, Yongqiang Yang, Lei Wang, Xinqiang Li, Qing Zhang, Rick F. Thorne, Wencai Li & Daoke Yang
Hyperthermia is a widely used adjunct treatment for different cancers including nasopharyngeal carcinoma (NPC). The protooncogene c-Myc is up-regulated in NPC and its expression is associated with poor prognosis. We hypothesized that c-Myc constitutes an important hyperthermia treatment target, and we investigated its contribution to hyperthermia responses in NPC. The growth of the human NPC cell lines CNE1 and CNE2 was analyzed using CCK-8 and clonogenicity assays after 43 °C hyperthermia, knockdown or overexpression of...

Additional file 1 of ETV2 promotes osteogenic differentiation of human dental pulp stem cells through the ERK/MAPK and PI3K-Akt signaling pathways

Jing Li, Haoran Du, Xin Ji, Yihan Chen, Yishuai Li, Boon Chin Heng & Jianguang Xu
Additional file 1: Figure S1. The mRNA level of CBFA1 in qRT-PCR (a) and RNA-Seq (b). *P < 0.05.

Additional file 4 of ETV2 promotes osteogenic differentiation of human dental pulp stem cells through the ERK/MAPK and PI3K-Akt signaling pathways

Jing Li, Haoran Du, Xin Ji, Yihan Chen, Yishuai Li, Boon Chin Heng & Jianguang Xu
Additional file 4: Figure S4. HE (scale bar = 50 μm) and Masson (scale bar = 50 μm) staining were performed to evaluate ectopic bone formation in nude mice (n=4).

Additional file 1 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 1: Figure S1. Panx 2 and Panx 3 mRNA expression after CCI. A-B. A qPCR assay was used to detect Panx 2 (A) and Panx 3 (B) mRNA levels at 4 and 14 days post-CCI. The data are mean ± SEM. n = 3 mice/group. **p < 0.01, ***p < 0.001, vs. sham injury. The data were analyzed by one-way ANOVA followed by Dunnett’s test.

Knockdown of long non-coding RNA plasmacytoma variant translocation 1 relieves ox-LDL-induced endothelial cell injury through regulating microRNA-30c-5p in atherosclerosis

Geng Li, Wenxia Zong, Lei Liu, Juan Wu & Jing Pang
Atherosclerosis (AS) is a chronic inflammatory disease involving endothelial dysfunction, and is one of the main causes of death from cardiovascular disease (CVD). Long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) is overexpressed in the serum of CVD patients. However, the mechanism by which lncRNA PVT1 functions in AS remains unknown. Our research was designed to probe interactions involving lncRNA PVT1 and oxidized low-density lipoprotein (ox-LDL)-stimulated endothelial cell injury in AS. lncRNA PVT1 expression...

Knockdown of long non-coding RNA plasmacytoma variant translocation 1 relieves ox-LDL-induced endothelial cell injury through regulating microRNA-30c-5p in atherosclerosis

Geng Li, Wenxia Zong, Lei Liu, Juan Wu & Jing Pang
Atherosclerosis (AS) is a chronic inflammatory disease involving endothelial dysfunction, and is one of the main causes of death from cardiovascular disease (CVD). Long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) is overexpressed in the serum of CVD patients. However, the mechanism by which lncRNA PVT1 functions in AS remains unknown. Our research was designed to probe interactions involving lncRNA PVT1 and oxidized low-density lipoprotein (ox-LDL)-stimulated endothelial cell injury in AS. lncRNA PVT1 expression...

Knockdown of zinc finger protein 267 suppresses diffuse large B-cell lymphoma progression, metastasis, and cancer stem cell properties

Hua Yang, Linmei Wang, Yingbin Zheng, Guiming Hu, Hongyan Ma & Liyun Shen
Zinc finger protein 267 (ZNF267) is a member of the Kruppel-like transcription factor family, which regulates various biological processes such as cell proliferation and differentiation. However, the biological significance of ZNF267 and its potential role in diffuse large B-cell lymphoma (DLBCL) remain to be documented. Experiments were herein conducted to study the role of ZNF267 in DLBCL. real-time quantitative reverse transcription PCR and Western blotting assays were conducted to detect the expression of ZNF267 in...

Ultrasound targeted microbubble destruction-mediated SOCS3 attenuates biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells

Xiaojiang Tang, Na Hao, Yuhui Zhou & Yang Liu
SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Additional file 4 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 4: Figure S1. Validation of ω-transaminase activity based on color reaction. 1: Control 2: Empty vector plasmid strain breaking solution 3: Breaking solution of strain expressing He-ωTA protein 4: Breaking solution of strain expressing Cv-ωTA protein 5: Purified He-ωTA protein solution 6: Purified Cv-ωTA protein solution.

Additional file 5 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 6 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Porcine epidemic diarrhoea virus (PEDV) infection activates AMPK and JNK through TAK1 to induce autophagy and enhance virus replication

Jingxiang Wang, Xianjin Kan, Xiaomei Li, Jing Sun & Xiulong Xu
Autophagy plays an important role in defending against invading microbes. However, numerous viruses can subvert autophagy to benefit their replication. Porcine epidemic diarrhoea virus (PEDV) is an aetiological agent that causes severe porcine epidemic diarrhoea. How PEDV infection regulates autophagy and its role in PEDV replication are inadequately understood. Herein, we report that PEDV induced complete autophagy in Vero and IPEC-DQ cells, as evidenced by increased LC3 lipidation, p62 degradation, and the formation of autolysosomes....

Knockdown of lectin-like oxidized low-density lipoprotein-1 ameliorates alcoholic cardiomyopathy via inactivating the p38 mitogen-activated protein kinase pathway

Yifan Zhang, Ruiqi Zhang, Lan Lu, Na Zhou, Xiaoyan Lv, Xin Wang & Zhanbin Feng
LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

Additional file 2 of Combining QTL mapping and RNA-Seq Unravels candidate genes for Alfalfa (Medicago sativa L.) leaf development

Xueqian Jiang, Xijiang Yang, Fan Zhang, Tianhui Yang, Changfu Yang, Fei He, Ting Gao, Chuan Wang, Qingchuan Yang, Zhen Wang & Junmei Kang
Additional file 2: Fig. S2 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of maternal parent (P2).

Additional file 4 of Identification of pyroptosis-related genes and long non-coding RNAs signatures in osteosarcoma

Jian Zhang, Jianjian Deng, Rui Ding, Jinghong Yuan, Jiahao Liu, Xiaokun Zhao, Tianlong Wu, Jingyu Jia & Xigao Cheng
Additional file 4: Fig. S2. The PPI network.

Additional file 3 of Exosomal miR-140-5p inhibits osteogenesis by targeting IGF1R and regulating the mTOR pathway in ossification of the posterior longitudinal ligament

Yifan Tang, Yanqing Sun, Junkai Zeng, Bo Yuan, Yin Zhao, Xiangwu Geng, Lianshun Jia, Shengyuan Zhou & Xiongsheng Chen
Additional file 3. Figure S2. Quantitative analysis of the phosphorylation of the proteins.

Additional file 3 of Exosomal miR-140-5p inhibits osteogenesis by targeting IGF1R and regulating the mTOR pathway in ossification of the posterior longitudinal ligament

Yifan Tang, Yanqing Sun, Junkai Zeng, Bo Yuan, Yin Zhao, Xiangwu Geng, Lianshun Jia, Shengyuan Zhou & Xiongsheng Chen
Additional file 3. Figure S2. Quantitative analysis of the phosphorylation of the proteins.

Additional file 3 of LPAR5 confers radioresistance to cancer cells associated with EMT activation via the ERK/Snail pathway

Xiao-Ya Sun, Hao-Zheng Li, Da-Fei Xie, Shan-Shan Gao, Xin Huang, Hua Guan, Chen-Jun Bai & Ping-Kun Zhou
Additional file 3: Figure S3. Significantly regulated PI3K pathway genes in HeLa cells 2 h after 4 Gy irradiation compared to unirradiated cells

Organoids from patient biopsy samples can predict the response of BC patients to neoadjuvant chemotherapy

Dan Shu, Meiying Shen, Kang Li, Xiaojian Han, Han Li, Zhaofu Tan, Yu Wang, Yang Peng, Zhenrong Tang, Chi Qu, Aishun Jin & Shengchun Liu
Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant chemotherapy treatment predicts survival. Studies have shown that patient-derived organoids can be used in cancer research and drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples to predict the response of patients to neoadjuvant chemotherapy. We generated a living organoid biobank from locally advanced breast cancer patients receiving neoadjuvant chemotherapy. When...

Organoids from patient biopsy samples can predict the response of BC patients to neoadjuvant chemotherapy

Dan Shu, Meiying Shen, Kang Li, Xiaojian Han, Han Li, Zhaofu Tan, Yu Wang, Yang Peng, Zhenrong Tang, Chi Qu, Aishun Jin & Shengchun Liu
Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant chemotherapy treatment predicts survival. Studies have shown that patient-derived organoids can be used in cancer research and drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples to predict the response of patients to neoadjuvant chemotherapy. We generated a living organoid biobank from locally advanced breast cancer patients receiving neoadjuvant chemotherapy. When...

Additional file 1 of Mucosal-associated invariant T cells predict increased acute graft-versus-host-disease incidence in patients receiving allogeneic hematopoietic stem cell transplantation

Zhao Wang, Sudong Zhang, Xiaoyu Zhang, Li Liu, Lukun Zhou, Yuyan Shen, Rongli Zhang, Yi He, Donglin Yang, Erlie Jiang, Xiaoming Feng, Jiaxi Zhou, Tao Cheng, Mingzhe Han & Sizhou Feng
Additional file 1: Other immune cell populations besides MAIT cells in grafts on aGVHD development.

Additional file 1 of Mucosal-associated invariant T cells predict increased acute graft-versus-host-disease incidence in patients receiving allogeneic hematopoietic stem cell transplantation

Zhao Wang, Sudong Zhang, Xiaoyu Zhang, Li Liu, Lukun Zhou, Yuyan Shen, Rongli Zhang, Yi He, Donglin Yang, Erlie Jiang, Xiaoming Feng, Jiaxi Zhou, Tao Cheng, Mingzhe Han & Sizhou Feng
Additional file 1: Other immune cell populations besides MAIT cells in grafts on aGVHD development.

Registration Year

  • 2022
    989

Resource Types

  • Image
    989

Affiliations

  • Chinese Academy of Medical Sciences & Peking Union Medical College
    989
  • Sichuan University
    632
  • Shanghai Jiao Tong University
    599
  • Fudan University
    571
  • Huazhong University of Science and Technology
    553
  • Zhejiang University
    549
  • Sun Yat-sen University
    518
  • Capital Medical University
    514
  • Nanjing Medical University
    494
  • Chinese Academy of Sciences
    475