Additional file 2: Figures S1. Confirmation of exosomes by transmission electron microscopy (TEM).

Additional file 2: Figures S1. Confirmation of exosomes by transmission electron microscopy (TEM).

Additional file 3: Fig. S3. Mapchart shown the distribution of GRAS genes on 7 chromosomes in woodland strawberry.

Additional file 8: Fig. S8. Mapchart shown the distribution of GRAS genes on 7 chromosomes in black raspberry.

Additional file 3: Fig. S2. A The expression levels of CEBPA-DT in different hepatoma cell lines measured by RT-qPCR. B Subcellular location of CEBPA-DT in indicated Huh7 cells examined by subcellular RNA fractionations and RT-qPCR analysis, β-actin and U3 were used as cytoplasmic and nuclear endogenous control, respectively. C The expression levels of CEBPA-DT in HCCLM9 and SNU-449 cells transfected with control siRNA or siRNA against CEBPA-DT. D qPCR quantification of the expression levels of...

Additional file 5: Fig. S4. A Clustering heatmap of significant differentially expressed mRNAs in HCCLM9 cells transfected with negative control or CEBPA-DT siRNAs. B Significantly enriched pathways annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Significantly enriched pathways annotated by the Gene Ontology (GO) database. D Significantly enriched pathways annotated by the Molecular Signatures Database (MSigDB). E The binding sites of hnRNPC and 3’UTR of DDR2 were predicted by PRIdictor database...

Additional file 7: Fig. S6. A-B Scratch wound-healing assays showed cell migration capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. C-D Transwell migration and matrigel invasion assays showed cell migration and invasion capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. E Representative images (fluorescence) of lung metastatic...

Aim: Diagnostic methods for HSV-1 need to be updated. Materials & methods: Serial dilutions of HSV- 1 DNA and ocular pathogenic microorganisms were tested by loop-mediated isothermal amplificationlateral flow dipstick assay (LAMP-LFD assay). The reliability of the LAMP-LFD assay and quantitative realtime PCR (qPCR) for HSV-1 detection was also validated using 30 clinical swab samples. Results: The LAMP reaction is optimal at 65◦C and 30 min. And the LAMP-LFD assay could detect HSV-1 up to...

SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

This study explored the function and mechanisms of HOX transcript antisense RNA (HOTAIR) in the drug resistance of nasopharyngeal carcinoma (NPC). Quantitative PCR, Western blotting, MTT assay, flow cytometry, Transwell assay, and luciferase assay were performed. HOTAIR expression levels were upregulated in cisplatin (DDP)-resistant NPC tissues and cells. Knockdown of HOTAIR in DDP-resistant NPC cells increased cell sensitivity of DDP, as well as decreased cell viability, expression of chemoresistance-related proteins, migration and invasion, increased cell...

Additional file 2.

Supplemental material, sj-tif-3-onc-10.1177_11795549221127161 for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery by Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang and Rui Yuan in Clinical Medicine Insights: Oncology

Supplemental material, sj-tif-5-tej-10.1177_20417314221132093 for A new cell-free therapeutic strategy for liver regeneration: Human placental mesenchymal stem cell-derived extracellular vesicles by Ting Li, Yu Fu, Zeyi Guo, Honglei Zhu, Hangyu Liao, Xiaoge Niu, Lin Zhou, Shunjun Fu, Yang Li, Shao Li, Lujia Wang, Yizhou Zheng, Lei Feng, Yi Gao and Guolin He in Journal of Tissue Engineering

Additional file 3. Tucidinostat plus checkpoint blockade induces improved therapeutic efficacy. a Schema of the experiment. For bioluminescence imaging (BLI) in vivo, mouse 4T1-luc (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). Tumors volume...

Additional file 2: Figure S1. (A) qRT-PCR analysis showed no significant changes in ACE2 expression in colorectal and pancreatic cancer cells after exposure to EPI, PTX or 5-FU. All data are shown as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns P > 0.05 versus control, N = 3.

Additional file 5: Figure S5. Percentage of integration sites at genomic features in each group with different clinical outcomes. Related to Figure 7.

Additional file 1: Fig. S1. Flowchart of the study design. A. Merge of WES cohorts from five published studies (Hellman et al. [10], Rizvi et al. [11], Miao et al [12, 13], Allen et al. [14], Liu et al. [15]). B. MSKCC cohort from the published study (Samstein et al [16]). C. The TCGA dataset was used to perform DDR-related gene mutation, tumor-infiltrating immune cells and prognostic analyses.

Additional file 2: Fig. S2. The pan-cancer landscape of MED12 mutations across human tumors. The proportion of MED12 mutated tumors identified for each cancer type with alteration frequency in TCGA pan-cancer cohorts.

Additional file 3: Fig. S3. Show the correlation of L_cancer RiskScore and L_cancer hub genes expression with immune infiltration level in L_cancer patients.

Additional file 2: Fig. S2 The progression-free survival (PFS) of patients receiving immune checkpoint inhibitor (ICI) treatment based on cancer types. The survival analysis was performed for individual cancer types that contained at least 10 cases in the SWI/SNF-mutant or SWI/SNF-non-mutant groups. The PFS of the SWI/SNF-mutant group was significantly superior to that of the SWI/SNF-non-mutant group in colorectal cancer (a) and gastric cancer (b), the same tendency was significant numerically by not statistically in...

Additional file 2.

Additional file 2: Fig. S2. (A) The comparison of immune infiltration levels between high-risk and low-risk groups in L_cancer patients, based on CIBERSORT. (B) The Stromal Score difference, Immune Score difference, ESTIMATE Score difference, and tumor purity difference between high-risk and low-risk groups in L_cancer patients. (C) The immune checkpoint-related gene expression levels in high-risk and low-risk groups in L_cancer patients. (D) The tumor mutation burden difference between high-risk and low-risk groups in L_cancer patients....

Additional file 4: Figure S4. Percentage of integration events at each genomic features based on gender and transduction efficiency. Related to Figure 6.