Sustained cardiac hypertrophy (CH) contributes to many heart diseases. Long noncoding RNAs (lncRNAs) collectively play critical roles in cardiovascular diseases (CVDs). However, the roles of lncRNA H19 in CH are still unclear. A CH model was constructed utilizing isoproterenol (ISO). We demonstrated H19 could participate in regulating ISO-induced CH development both in vivo and in vitro. The online databases DIANA and TargetScan were used to predict the targets of H19 and MicroRNA-145-3p (miR-145-3p), respectively. Luciferase...

Additional file 11: Figure S2. circPVT1 promotes the migration and invasion of NPC in vitroand metastasis of NPCin vivo. A-B. The expression of circPVT1 were measured in CNE2 and 5-8F cells after transfection with the circPVT1 overexpression vector or circPVT1 siRNA. PVT1 expression was not affected in CNE2 and 5-8F cells after overexpresion or knockdown of circPVT. All experiments were repeated at least three times. Data were represented as mean ± SD. ***, p <...

Additional file 11: Figure S2. circPVT1 promotes the migration and invasion of NPC in vitroand metastasis of NPCin vivo. A-B. The expression of circPVT1 were measured in CNE2 and 5-8F cells after transfection with the circPVT1 overexpression vector or circPVT1 siRNA. PVT1 expression was not affected in CNE2 and 5-8F cells after overexpresion or knockdown of circPVT. All experiments were repeated at least three times. Data were represented as mean ± SD. ***, p <...

Additional file 12: Figure S3. circPVT1 promotes the migration and invasion of NPC cells through binding to β-TrCP. A. The secondary ion flow diagram of β-TrCP identified by mass spectrometry according to the peptide sequence (DFITALPAR) of β -TrCP.B. Schematic diagram of binding sites betweent circPVT1 and β-TrCP using the catRAPID website.The 230-280 nt of circPVT1 was predicted to bind to β-TrCP protein with high interaction score.C. The secondary structure of circPVT1 changed when 230-280...

Additional file 13: Figure S4. The WD40 repeat domain of β-TrCP interacts with circPVT1. A. The functional domain of the of β-TrCP proteins are illustrated (top), including the F-box domain and the WD40 domain. The expressing plasmids for full-length β-TrCP or truncated fragments (F-box and WD40) were constructed and confirmed using anti-Flag antibody. B. Wound healing assay showed that overexpression of β-TrCP inhibited NPC cells migration. Data were represented as mean ± SD. ***, p...

Additional file 14: Figure S5. c-Myc is ubiquitinated substrate of β-TrCP. A. The expression of β-TrCP was examined in CNE2 and 5-8F cells after overexpression or knockdown of circPVT1 using western blotting.B. The β-TrCP binding proteins were identified in CNE2 cells by immunoprecipitation followed by LC-MS/MS.C. The interaction of c-Myc and β-TrCP was predicted by Molecular Docking ( http://hdock.phys.hust.edu.cn/ ). The blue part is the WD40 repeat domain of β-TrCP.D. The expression of c-Myc was...

TableS1. Clinical data of ovarian cancer patients from TCGA and ICGC.
TableS2.Identified DNA driver methylation (DNAme) in TCGA, ICGC and GSE146556.
TableS3. The correlation between DNAme and corresponding mRNA expression in TCGA, ICGC and GSE146556. TableS4.Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of DNAme-associated mRNA in TCGA.
TableS5. Univariate Cox regression analysis of DNAme-associated mRNA. (P < 0.05)
TableS6. Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of...

Additional file 9: Fig. S4. Whole-genome variation of two parental lines of QYSM. a, Density and chromosome distribution of polymorphic SNPs between Q9311B and WSSM. b, The principal component analysis. c, Phylogenetic positions of two parental lines (Q9311B and WSSM) in the neighbor-joining tree of 1,946 rice accessions from 3 K-RG, in which the known information on the clades of 3 K-RG is indicated.

Additional file 2: Figure S2. CEF3 amino acid sequence alignment of WT and cef3 mutant

Additional file 3: Figure S3. Complementary assay. The phenotype (A) and the plant height (B) show the rescued properties in the complemented plants. Scale bars: 10 cm.

Additional file 1: Fig. S1 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of paternal parent (P1).

Additional file 3: Supplementary figure 3. The expression of TRIM34, SLC30A10, HOXD4 and circ_0007675 in CLL patients and normal B cells.(A) The expression of TRIM34 in patient specimens were significantly increased. (B) The expression of SLC30A10 in patient specimens were significantly increased. (C) The expression of HOXD4 in patient specimens were significantly increased. (D) The expression of circ_0007675 in patient specimens were significantly increased. All results are expressed as mean ± SEM.

Additional file 2. Figure S1: Results of RNA-seq of the A549 cell line. A. The volcano plot of differentially expressed genes revealed by RNA-seq sequencing. B. Heatmap for autophagy- or interferon-related gene expression. C. Enrichment analysis for differentially expressed genes based on the GO database. D. Enrichment analysis for differentially expressed genes based on the REACTOM database.

Additional file 2. Figure S1: Results of RNA-seq of the A549 cell line. A. The volcano plot of differentially expressed genes revealed by RNA-seq sequencing. B. Heatmap for autophagy- or interferon-related gene expression. C. Enrichment analysis for differentially expressed genes based on the GO database. D. Enrichment analysis for differentially expressed genes based on the REACTOM database.

Additional file 11: Figure S5. Expression of the predicted AI-2 synthases and receptors within a rumen microbial metatranscriptome at the phylum level. (A) The number of bacterial genomes containing AI-2-related genes and the percentage of corresponding genomes that expressed these genes. (B) The number of bacterial genomes containing luxS synthases and the percentage of the corresponding genomes that expressed luxS genes at the phylum level. (C) The number of bacterial genomes containing lsrB receptors and...

Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 4: Figure S4. (A-C) CFP-103Q-HeLa cells were treated with 20 μM of J3 for 48 h. 10 μM of CHX was added at the last 6 h. The protein expression of mHTT (anti-GFP, sc-9996) was detected by western blot (A), and the quantification of insoluble mHTT (B) and soluble mHTT (C) was analyzed. (D-F) CFP-103Q-HeLa cells were treated with 20 μM of J3 for 48 h. 40 μM of CQ was added at...

Additional file 4: Figure S4. (A-C) CFP-103Q-HeLa cells were treated with 20 μM of J3 for 48 h. 10 μM of CHX was added at the last 6 h. The protein expression of mHTT (anti-GFP, sc-9996) was detected by western blot (A), and the quantification of insoluble mHTT (B) and soluble mHTT (C) was analyzed. (D-F) CFP-103Q-HeLa cells were treated with 20 μM of J3 for 48 h. 40 μM of CQ was added at...

Additional file 6: Figure S2. Distribution and abundance of AI-2 synthases and receptors based on QS in the rumen bacterial genomes. Numbers indicate the number of bacterial genomes in which the corresponding proteins were found at the genus level. The LuxS synthases, LsrB receptors, and CahR-type receptors were discovered within 88, 27, and 42 genera, respectively.

Additional file 9: Figure S4. The types of the CahR-type receptors. Numbers indicate the number of bacterial genomes in which the corresponding proteins were found. The MCPs and HKs were two dominant AI-2 receptor proteins in rumen bacteria.

Additional file 9: Figure S2. Functional heterogeneity of CRC epithelial cells in the SMC cohort. A. UMAP feature plot of the top 10 upregulated genes in the SMC cohort. B. Correlation heat map of 123 programs identified by cNMF. The Pearson correlation coefficient is indicated by the color. C. Heatmap of the relative mean signature score of cancer cell functional signatures across C1-C7. The signature score was calculated using the “AUCell” package. D. Heatmap of...

Additional file 2: Figure S1. Depletion of LARRPM promoted proliferation, repressed apoptosis and promoted migration and invasion of LUAD cells. A LARRPM expression in HCC827 cells with LARRPM stable depletion or control was detected by qRT-PCR. B Cell proliferation of HCC827 cells with LARRPM stable depletion or control was detected using CCK-8 assays. C Cell proliferation of HCC827 cells with LARRPM stable overexpression or control was detected using EdU incorporation assays. Scale bar: 100 µm....

Additional file 3: Figure S2. The correlation between LARRPM expression, 5hmC level at LINC00240 promoter, CpG43 methylation level and LINC00240 expression in LUAD tissues. A Correlation between LINC00240 and LARRPM expression analysed using the TCGA LUAD data. r = 0.6164, P < 0.0001 by Spearman correlation analysis. B Correlation between LINC00240 and LARRPM expression analyzed in our LUAD cohort. r = 0.5521, P < 0.0001 by Spearman correlation analysis. C 5hmC levels of CpG43 from...

Additional file 4: Figure S3. LINC00240 repressed proliferation, induced apoptosis and inhibited migration and invasion of LUAD cells. A LINC00240 expression in A549 cells with LINC00240 stable overexpression or control was detected by qRT-PCR. B Cell proliferation of A549 cells with LINC00240 stable overexpression or control was detected using CCK-8 assays. C Cell proliferation of A549 cells with LINC00240 stable overexpression or control was detected using EdU incorporation assays. Scale bar: 100 µm. Red color...