The sodium channel NaV1.5, which is encoded by the SCN5A gene, underlies the fast upstroke of cardiac action potential and thus plays a crucial role in cardiac electrophysiology, but the mechanism governing the regulation of NaV1.5 has not been fully elucidated. The newly developed clustered regularly interspaced short palindromic repeats (CRSPR)/Cas9 transcription factors offer a powerful and precise approach for modulating gene expression. We investigated the potential of this new tool for activating stringently silenced...

Additional file 6: Figure S2. miRNAs with similar expression patterns in crown and leaf tissues at the winter dormancy stage, spring green-up stage and jointing stage.

Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Long non-coding RNA (lncRNA) ZFAS1 (zinc finger antisense 1) was demonstrated to play critical roles in various cancer progression. However, the functions of ZFAS in cervical cancers (CC) are unclear. Human CC cell lines were used for in vitro experiments. RT-qPCR (Real Time Quantitative PCR) was performed to detect the expression of ZFAS1, microRNA-190a-3p (miR-190a-3p) and Kruppel-like factor 6 (KLF6). Cell proliferation, invasion and migration assays were used to investigate biological behaviors of CC cells...

Additional file 9: Fig. S4. Whole-genome variation of two parental lines of QYSM. a, Density and chromosome distribution of polymorphic SNPs between Q9311B and WSSM. b, The principal component analysis. c, Phylogenetic positions of two parental lines (Q9311B and WSSM) in the neighbor-joining tree of 1,946 rice accessions from 3 K-RG, in which the known information on the clades of 3 K-RG is indicated.

Additional file 10: Fig. S10. The association between the protein secretion and prognosis in pan-cancer cohort.

Additional file 1: Fig. S1. Multi-omics analysis of CRGs. (A) Differential methylation between tumor and normal samples of CRGs in pan-cancer; FDR count: the significance of FDR; FDR: adjust p-value; Methy.diff(T-N): differential methylation (Tumor vs. Normal) (B) Co-occurrence and mutually exclusive of CRGs in pan-cancer.

Additional file 1: Fig. S1 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of paternal parent (P1).

Additional file 3: Supplementary figure 3. The expression of TRIM34, SLC30A10, HOXD4 and circ_0007675 in CLL patients and normal B cells.(A) The expression of TRIM34 in patient specimens were significantly increased. (B) The expression of SLC30A10 in patient specimens were significantly increased. (C) The expression of HOXD4 in patient specimens were significantly increased. (D) The expression of circ_0007675 in patient specimens were significantly increased. All results are expressed as mean ± SEM.

Supplementary Material 6

sj-tif-10-cll-10.1177_09636897221129171 for Effects of Low-Intensity Pulsed Ultrasound on the Migration and Homing of Human Amnion–Derived Mesenchymal Stem Cells to Ovaries in Rats With Premature Ovarian Insufficiency by Li Ling, Jiying Hou, Yan Wang, Han Shu and Yubin Huang in Cell Transplantation

sj-tif-10-cll-10.1177_09636897221129171 for Effects of Low-Intensity Pulsed Ultrasound on the Migration and Homing of Human Amnion–Derived Mesenchymal Stem Cells to Ovaries in Rats With Premature Ovarian Insufficiency by Li Ling, Jiying Hou, Yan Wang, Han Shu and Yubin Huang in Cell Transplantation

sj-tif-12-cll-10.1177_09636897221129171 for Effects of Low-Intensity Pulsed Ultrasound on the Migration and Homing of Human Amnion–Derived Mesenchymal Stem Cells to Ovaries in Rats With Premature Ovarian Insufficiency by Li Ling, Jiying Hou, Yan Wang, Han Shu and Yubin Huang in Cell Transplantation

sj-tif-6-cll-10.1177_09636897221129171 for Effects of Low-Intensity Pulsed Ultrasound on the Migration and Homing of Human Amnion–Derived Mesenchymal Stem Cells to Ovaries in Rats With Premature Ovarian Insufficiency by Li Ling, Jiying Hou, Yan Wang, Han Shu and Yubin Huang in Cell Transplantation

Additional file 1: Supplemental Figure 1. The cytotoxicity of B7-H3CAR-T cells against solid tumor cell lines was analyzed by RTCA assay. The redarrow represents the time point of effector cell addition.

Additional file 11: Figure S5. Expression of the predicted AI-2 synthases and receptors within a rumen microbial metatranscriptome at the phylum level. (A) The number of bacterial genomes containing AI-2-related genes and the percentage of corresponding genomes that expressed these genes. (B) The number of bacterial genomes containing luxS synthases and the percentage of the corresponding genomes that expressed luxS genes at the phylum level. (C) The number of bacterial genomes containing lsrB receptors and...

Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 10. Detailed region where the representative images of Figure 5A selected from. LC, lesion cord.

Additional file 9. Detailed region where the representative images of Figure 2A selected from. LC, lesion cord.

Additional file 2: Fig. S2. Ubiquitous knockdown of serotonin receptors has no effect on the climbing ability of flies. (A) qRT-PCR analysis of the mRNA expression of all five serotonin receptor knockdown lines. (B) Climbing ability of serotonin receptor knockdown flies detected by a negative geotaxis assay. Error bars are shown as the mean ± SEM. For all data, *p<0.05, **p<0.01, ***p<0.001.

Additional file 3: Fig. S3. Verification of 5-HT2B knockout flies. (A) Molecular verification of 5-HT2B knockout flies by PCR analysis. Only one band was seen in w1118 controls, but a lower band could exist when a fragment of approximately 233 bp was deleted from the 5-HT2B locus. (B) The PCR-Sanger sequencing results of two reserved homozygous 5-HT2B knockout lines. The results show that the 5-HT2BKO_5-1 line has a 224 bp deletion in the first exon...

Additional file 6: Figure S2. Distribution and abundance of AI-2 synthases and receptors based on QS in the rumen bacterial genomes. Numbers indicate the number of bacterial genomes in which the corresponding proteins were found at the genus level. The LuxS synthases, LsrB receptors, and CahR-type receptors were discovered within 88, 27, and 42 genera, respectively.