Additional file 1: Supplementary Fig. 1. YTHDF2 knockdown by siYTHDF2-2 promoted GC cell proliferation and migration. A Western blot result showing the knockdown efficiency of YTHDF2 in HGC27 cells. B YTHDF2 knockdown promoted cell viability of HGC27 cells via CCK8 assay. C YTHDF2 knockdown promoted HGC27 cell migration via transwell assay.

Additional file 2: Supplementary Fig. 2. The functional link between YTHDF2 and PPP2CA in GC cells. A Cell migration capacity was determined by transwell assay in YTHDF2 overexpression cells transfected with siPPP2CA-2 and siNC. Western blot confirmed the knockdown efficiency of PPP2CA. B PPP2CA overexpression suppressed the phenotype induced by YTHDF2 knockdown in AGS cells via transwell assay. FLAG-tagged PPP2CA was detected by western blotting.

Additional file 7: Fig. S7. Structure of nirK and nirS communities in the absence or presence of AMF. Pot expt 1. Relative abundance of major taxonomic groups of nirK and nirS communities under the −AMF and +AMF treatments in different patches (n = 8). Control, soil patch; NSfaba and Sfaba, patches with unsterilized (NS) or sterilized (S) faba bean residues, respectively; asterisks, significant differences between the −AMF and +AMF treatments in each patch type according...

Additional file 6: Fig. S6. Soil water content, total carbon and nitrogen contents, mineral nitrogen and dissolved total nitrogen contents in patches in the absence or presence of AMF A-C, pot expt 1. Soil water content (A), total carbon (B) and nitrogen (C) contents under the −AMF and +AMF treatments in different patches (n = 8). Control, soil patch; NSfaba and Sfaba, patches with unsterilized (NS) or sterilized (S) faba bean residues, respectively. D-G, pot...

Additional file 7: Figure S7. Representative FACS plots and bar graphs showing CD45R/B220+ B cells in the spleens of B-NDG mice in non-IR + PBS, IR + PBS, IR + GA, IR-OEa8 + PBS, and IR-OEa8 (PM69) + GA groups.

Additional file 2. Figure S2：Immunohistochemistry of H4M regulators. A-H The protein levels of H4M regulators in normal liver and LIHC were visualized by immunohistochemistry in HPA.

Additional file 1: Fig S1. The mutational landscape of our AML cohort. (A) Frequent mutations with more than 10 counts in our cohort were showed. (B) The relationship between mutations was analyzed, concurrent and mutually-exclusive mutations were indicated. (C) The concurrent or mutually-exclusive mutations for common rearrangements in AML were exhibited.

Additional file 3: Figure S1. The cell viability of MSC or MSCATV after 72h treatment measured by MTT assay. ns: no significance.

Additional file 10: Figure S7. Stat1 is the target gene of miR-139-3p. A, Consequential pairing of Stat1 3’UTR and miR-139-3p predicted by TargetScan. B, Luciferase activity was measured and normalized by Renilla luciferase activity. WT, wild type. Mut, mutated. NC, negative control. **P<0.01 compared with WT 3’UTR+NC mimic group, n = 4 per group.

Additional file 2: Figure S2. The standard IHC PD L1 scores in Adeno-NEPC.

Additional file 3: Figure S3. Co expression of CgA, SYN, CD56 and NSE in Adeno-NEPC.

Additional file 12. Gels and blots of Figure 1A.

Additional file 12. Gels and blots of Figure 1A.

Additional file 3: Figure S3. IPA network pathway analysis. The IPA network pathway analysis revealed that these differentially expressed lipids were related to NF-κB signaling, PI3K/AKT signaling, and JAK/STAT signaling. IPA, ingenuity pathway analysis; CP, classical signaling pathway.

Additional file 1. Representative images of clinical and autopsy changes. The severity of heart and spleen lesions were assessed to evaluate the immune protective efficacy against APEC O2 challenge. Gross lesions were evaluated visually.

Additional file 2. Fig. S2. Additional materials for GSEA analysis. A. Other cell proliferation/differentiation-related GSEA analysis results in target genes of active regulons in preosteoblast-S1. B. Other immunity-related GSEA analysis results in target genes of active regulons in preosteoblast-S1.

Additional file 2: Figure S2. The standard IHC PD L1 scores in Adeno-NEPC.

Additional file 6: Figure S3. (a) Normalized enrichment scores for GSEA analysis between baseline and post-treatment tumor samples in response group. (b) Increasing trend of T cells after treatment in response tumor samples. (c) Neutrophils showed a decreasing tendency after treatment in response lymph node samples. (d) Neutrophils related gene set was significantly down-regulated after treatment in response lymph node samples.

Additional file 7. Figure S7: Immune infiltration evaluation and WGCNA analysis process. A-B Evaluating tumor-infiltrating immune cell abundance differences among three H4Mclusters. C-E The detailed analysis process of identifying key modules by WGCNA analysis. F Scatterplots of correlation between MEturquoise membership and gene significance for H4Mcluster-C. G Expression of 36 H4M regulators among three H4M geneClusters. H-J Differences in intratumor heterogeneity score, HRD score, and proliferation score among three H4M geneClusters. *, **, and ***...

Additional file 1: Supplementary Figure 1. Expression of circHMCU in clinical samples and selection of miR-4458 and PGK1. (A) Expression of eight miRNAs by qRT-PCR in MCF-7 cells transfected with si-circHMCU or si-NC. (B) Expression of six mRNAs by qRT-PCR in MCF-7 cells transfected with mimic NC or miR-4458 mimic. *P < 0.05.

Additional file 1.

Additional file 1. Eukaryotic expression of viral proteins in HEK293T cells. HEK293T cells were transfected with pCAGGS-C-HA, pCAGGS-prM-HA, pCAGGS-NS2A-HA and pCAGGS-NS4B-HA respectively for 48 h. Samples were harvested for Western blotting analysis and immunoblotted for the proteins HA. Bands labeled by “★” indicated the expressed specific viral proteins.

Additional file 2.

Additional file 3: Supplementary Fig. 2. (A) Detection of intracellular Fe2+ concentrations in Huh7 and 7721 cells after knockdown of FASN, after overexpression of FASN, and after 24 h incubation with inhibitor orlistat. (B) Detection of intracellular GSH concentrations in Huh7 and 7721 cells after knockdown of FASN, after overexpression of FASN, and after 24 h incubation with inhibitor orlistat. (C) Detection of MDA levels in Huh7 and 7721 cells after knockdown of FASN, after...

Additional file 4: Supplementary Fig. 3. (A) Effect of overexpression of HIF1α on lipid peroxide levels in FASN-knockdown and control Huh7SR and 7721SR cells exposed to sorafenib. (B) Effect of overexpression of HIF1α on MDA concentrations in FASN-knockdown and control Huh7SR and 7721SR cells exposed to sorafenib. (C) Lipid-peroxidation levels in Huh7SR and 7721SR cells after incubated with 2.5 µM AA and 10 µM LA for 24 h. (D) MDA concentrations in Huh7SR and 7721SR...