Additional file 1: Supplementary Figure 1. OC cell lines exhibit heterogenous biosensor responses to OCM treatment. Fluorimetric measurements of iNap (A), Perceval (B), and Peredox (C) signal were normalized to the average across all cell lines (n = 3 technical replicates per cell line). Signal was measured following 48 hours of growth in OCM or regular media and normalized to growth in control regular media for iNap (D), Perceval (E), and Peredox (F).

Additional file 2: Supplementary Figure 2. Biosensor responses to carboplatin treatment across heterogenous OC cell lines are negatively correlated with carboplatin resistance. Biosensor-expressing cell lines were treated with 100 μM carboplatin and iNap (A), Perceval (B), and Peredox (C) signal was measured at 24 and 48 hours. (D-F) Signal for each biosensor in (A-C) in treated cells was compared to that in control, untreated cells, normalized to 1 (n = 3 per condition). Normalized iNap...

Additional file 1: Figure S1. Validation of TPM1 overexpression or knockdown in BV2 cells after transfection with TPM1 plasmid or siTPM1. A mRNA level of TPM1 in BV2 cells after TPM1 plasmid transfection. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test or by one-way ANOVA with Tukey’s multiple comparison test (control plasmid vs. TPM1 plasmid, **p < 0.01). Three independent experiments were performed. B, C Western blot analysis...

Additional file 2: Figure S2. TPM1 knockdown reduces inflammation via the PKA/CREB pathway in BV2 cells. A–F qPCR analysis of TPM1, TNF-α, IL-1β, IL-6, COX-2 and iNOS in BV2 cells following treatments with LPS and siTPM1-1, siTPM1-2, or siCTR. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to LPS + siCTR or control, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001;...

Additional file 3: Figure S3. TPM1 knockdown reduces LPS-induced inflammation and function decline in C57BL/6J mice. A, B mRNA level of TPM1 (A) and IL-10 (B) in C57BL/6 J mouse retinas after treatments with LPS and siCTR or siTPM1-1. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to LPS + siCTR or LPS + siTPM1-1, *p < 0.05, **p < 0.01). n = 4 or...

Additional file 6: Figure S6. TPM1 knockdown elicits inflammation-related transcriptomic alterations in the WT retina. A, B DEGs associated with the phagosome formation pathway (A) and neuroinflammation signaling pathway (B) in WT mice after treatments with LPS and siTPM1-1 or siCTR.

Additional file 10: Supplementary Figure 6. A Image of subcutaneous tumors of four groups implanted with LLC-circZNF451: WT + PBS, WT + PD1 blockade, ELF4 KO + PBS, and ELF4 KO + PD1 (n = 5 for each group) and the statistical analysis on the tumor growth of the four groups. The conditional knockout of ELF4 is accomplished with C57BL/6J-Lyz2CreERT2 and C57BL/6J-ELF4em1(flox)Smoc mice; KO, knockout. B TIM3 expression and GZMB secretion in CD8+ T cells...

Additional file 14. Figure S14. Reclustering analysis of tumor-infiltrating NK cells. (a) T-distributed stochastic neighbor embedding (t-SNE) plot for NK cells. (b) t-SNE plot for the treatment group-specific distribution of NK cell subclusters. (c) Violin plots showing NK cell subcluster-specific gene profiles. (d) Histogram representing the proportion of NK cells subcluster in each group. (e-f) Violin plots showing the levels of Cd69 and Tnf of NK cell subclusters. (g) Heatmap depicting the results of GSEA...

Additional file 2. Figure S2: Flow cytometry for daughter CD4+ T cell in one-way mixed lymphocyte reaction (MLR). Stimulating cells were BMDCs derived from BALB/c mice while responding cells were spleen cells from C57BL/6 in the MLR assays. The mixed cells with YM101 or control antibodies were cultured for four days. On day 5, cells were collected for CFSE dilution assay.

Additional file 2. Figure S2: Flow cytometry for daughter CD4+ T cell in one-way mixed lymphocyte reaction (MLR). Stimulating cells were BMDCs derived from BALB/c mice while responding cells were spleen cells from C57BL/6 in the MLR assays. The mixed cells with YM101 or control antibodies were cultured for four days. On day 5, cells were collected for CFSE dilution assay.

Additional file 6. Figure S6: The effect of the combination therapy on T cell infiltration and peritumoral collagen deposition in the EMT-6 model. (a) Immunofluorescent staining showing T cells in tumor margin and center. (b) Picrosirius red staining showing picrosirius red staining.

Additional file 7. Figure S7: The FACS gating strategies for the B16 model.

Additional file 7. Figure S7: The FACS gating strategies for the B16 model.

Additional file 5: Supplementary Figure 1. A The enrichment of 4 most significantly changed circRNAs (FC > 4, P < 0.05) of the circRNA sequencing in the exosomes of 6 LUAD patients (3 PR and 3 PD) accepting the PD1 blockade was measured by qRT-PCR. B Correlation between exosomal circRNA-seq results of circZNF451 and the expression of circZNF451 in the tumor tissues of six LUAD patients was analyzed by Spearman correlation analysis. C The expression...

Additional file 6: Supplementary Figure 2. A Infiltration of CD4+T, CD8+T, Foxp3+Treg, CD56+NK and CD11C+dendritic cells and CD86+ and CD163+ macrophages in the circZNF451high and circZNF451low group was presented in the violin plot. B The transfection efficiency of circZNF451 in LLC was measured by qRT-PCR. C The construction of A549-shcircZNF451, H1299-shcircZNF451, H1395-circZNF451 and H1975-circZNF451 cell lines was confirmed by qRT-PCR. D IFN-γ and GZMB levels in the supernatant of CD8+ T cells were measured via...

Additional file 7: Supplementary Figure 3. A Silver staining of the complexes precipitated by the biotinylated circZNF451/NC probe. The red arrow marks the specific band of the circZNF451 probe. B The overlap of the mass spectrometry data, the predicted candidates for circZNF451 in circInteractome and the most detected binding motifs for circZNF451 in catRAPID database. C After coculturing with H1395/H1975-shcircZNF451 and GW4869 (20μM), FXR1 expression in macrophages was detected via western blotting. D and E...

Supplementary Material 7

Additional file 2: Figure S2. Consensus clustering analysis of DEGs in GC. (A) The cumulative distribution function (CDF) curves in consensus cluster analysis for cluster numbers k = 2–9. (B) Relative change in the area under the CDF curve from k = 2 to k = 9. (C) The tracking plot for k = 2–9; The vertical axis shows consensus matrix k-value, the horizontal axis represents the GC samples. (D-L) Consensus matrix heat map when...

Supplementary figures 1-5 Supplementary tables 1-8

Supplemental Material for Fiber reinforced polypropylene composites interfacial behavior improvement fabricated by cold plasma jet SiOx nanoparticles deposition by Lin Zhang, Zhangchuan Xia, Yadong He, Chunling Xin, Yang Yu, Feng Ren and Ruixue Wang in Journal of Composite Materials

Supplemental Material for Fiber reinforced polypropylene composites interfacial behavior improvement fabricated by cold plasma jet SiOx nanoparticles deposition by Lin Zhang, Zhangchuan Xia, Yadong He, Chunling Xin, Yang Yu, Feng Ren and Ruixue Wang in Journal of Composite Materials

Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

Triggering receptor expressed on myeloid cells 1 (TREM1) participates in the development of endometritis. This study aims at identifying the effects and interaction of TREM1 and upstream stimulatory factor 2 (USF2) in endometritis by using a model of lipopolysaccharide (LPS)-induced human endometrial epithelial cells (HEnEpCs). ELISA was performed to determine the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF-α) after LPS stimulation. TREM1 and USF2 expression was examined with RT-qPCR and Western blot....

Ulcerative colitis (UC) is the most prevalent form of chronic inflammatory bowel disease, the etiology of which is poorly understood. This study investigated the role of miR-151-5p on UC and explored the role of brain-derived neurotrophic factor (BDNF) in a UC mouse model and cell model. A UC mouse model was engineered by dextran sulfate sodium (DSS) induction. Primary mouse intestinal epithelial cells (IECs) were isolated. Colitis mice were intraperitoneally injected with miR-151-5p antagomir and...

Supplemental material, sj-jpg-3-tej-10.1177_20417314221147113 for Construction of a pancreatic cancer nerve invasion system using brain and pancreatic cancer organoids by Chenyun Song, Xinyu Chen, Jixin Ma, Hada Buhe, Yang Liu, Hexige Saiyin and Lixiang Ma in Journal of Tissue Engineering