Additional file 2: Figure S2. Effects of manipulating androgen production in ex vivo cultured human fetal testis tissue on expression of the Sertoli cell marker SOX9. Expression pattern of the Sertoli cell marker SOX9 in fetal testis samples treated with ketoconazole (10-6 M) and hCG (1 IU/ml) for two weeks in ex vivo culture. Images representative for the expression in each age-group and treatment. Counterstaining with Mayer haematoxylin, scale bar corresponds to 50 µm.

Additional file 4: Figure S4. Effects of reduced androgen action via blocking of the androgen receptor in ex vivo culture of human fetal testes. Expression pattern of CYP11A1 and androgen receptor (AR) in fetal testis samples treated with flutamide (10-6 M) for two weeks in ex vivo culture. Images representative for the expression in samples aged GW 7-12. Counterstaining with Mayer haematoxylin, scale bar corresponds to 50 µm.

Additional file 1: Figure S1. Effects of manipulating androgen production or androgen exposure via blocking of the androgen receptor in ex vivo culture of human fetal testes. Expression of the proliferation marker (BrdU) and apoptosis marker (cPARP) in fetal testis samples treated with ketoconazole (10-6 M) and flutamide (10-6 M) for two weeks in ex vivo culture. Images representative for the expression in samples aged GW 7-12. Counterstaining with Mayer haematoxylin, scale bar corresponds to...

Additional file 1: Fig. 1. Predicted probability of death across the 6 hypothermia groups as compared to the normothermia groups at the corresponding sites. Patients in group 1, with the lowest average temperature at 4 hours had a higher probability of death.

Additional file 2: Figure S1. Birth weight percentiles by sex and gestational age for STOPPAM vs. Intergrowth (IG21) references. The percentiles before and after 33 weeks for Intergrowth were merged, hence the bend in the 10th percentile.

Additional file 2: Figure S1. Birth weight percentiles by sex and gestational age for STOPPAM vs. Intergrowth (IG21) references. The percentiles before and after 33 weeks for Intergrowth were merged, hence the bend in the 10th percentile.

Additional file 3: Figure S2. The birth weight z-scores comparing STOPPAM vs. Intergrowth (IG21) references. The dash line indicates the mean birthweight z-scores.

Additional file 4: Figure S3. Association between small for gestational age (SGA) and malaria in pregnancy excluding all HIV seropositive women. Panels A and B shows the unadjusted odds ratio (uOR) for SGA when using STOPPAM (SGASTOPPAM) and Intergrowth-21 (SGAIG21) references. Panels C and D shows the adjusted odds ratio (aOR).

Additional file 3: Figure S3. Effects of reduced androgen exposure via blocking of the androgen receptor in ex vivo culture of human fetal testes. Quantification of A) testosterone B) androstenedione and C) DHEAS secretion by ex vivo cultured fetal testis tissue from GW 7-12 following treatment with flutamide (10-6 M) and ketoconazole (10-6 M) for 14 days. Media were collected every 48 hours throughout the 14-day culture period and were pooled for each individual tissue...

Additional file 5: Figure S4. Two stage individual participant data meta-analysis on the association between malaria in pregnancy (MIP) and small for gestational age (SGA) using the STOPPAM (SGASTOPPAM) vs. the Intergrowth-21 reference (SGAIG21). uOR: unadjusted odds ratio in panels A and B, aOR: adjusted odds ratio in panels C and D controlling for body mass index, gravidity, gestational age at enrolment, HIV, and hemoglobin level at enrolment. In addition, adjusted for gestational age at...

Additional file 5: Figure S4. Two stage individual participant data meta-analysis on the association between malaria in pregnancy (MIP) and small for gestational age (SGA) using the STOPPAM (SGASTOPPAM) vs. the Intergrowth-21 reference (SGAIG21). uOR: unadjusted odds ratio in panels A and B, aOR: adjusted odds ratio in panels C and D controlling for body mass index, gravidity, gestational age at enrolment, HIV, and hemoglobin level at enrolment. In addition, adjusted for gestational age at...

Additional file 6: Figure S5. Funnel plots for meta-analysis. The closed dots indicate the observed studies, panel A indicate the trim and fill funnel plot for STOPPAM (P = 0.10) and panel B for Intergrowth-21 reference (P = 0.35). The contour enhanced funnel plot for STOPPAM (panel C) and Intergrowth-21 (panel D) show the distribution of studies in both the small and large p-values contours, hence no publication bias.

Additional file 4: Figure S4. Effects of reduced androgen action via blocking of the androgen receptor in ex vivo culture of human fetal testes. Expression pattern of CYP11A1 and androgen receptor (AR) in fetal testis samples treated with flutamide (10-6 M) for two weeks in ex vivo culture. Images representative for the expression in samples aged GW 7-12. Counterstaining with Mayer haematoxylin, scale bar corresponds to 50 µm.

Objectives. Heart transplantation (HTx) has become an established treatment option in patients with end-stage heart failure. The aim of this study was to report on long-term outcome over the past three decades. Design. Consecutive adult patients receiving first-time and isolated HTx from October 3, 1990, to November 2, 2020, at Rigshospitalet, Copenhagen, Denmark, were retrospectively evaluated. Data were obtained from the Scandinavian Transplant Registry and patient medical records. Recipients were grouped by time of transplantation...

Additional file 1: Figure S1. Effects of manipulating androgen production or androgen exposure via blocking of the androgen receptor in ex vivo culture of human fetal testes. Expression of the proliferation marker (BrdU) and apoptosis marker (cPARP) in fetal testis samples treated with ketoconazole (10-6 M) and flutamide (10-6 M) for two weeks in ex vivo culture. Images representative for the expression in samples aged GW 7-12. Counterstaining with Mayer haematoxylin, scale bar corresponds to...

Additional file 4: Figure S3. Association between small for gestational age (SGA) and malaria in pregnancy excluding all HIV seropositive women. Panels A and B shows the unadjusted odds ratio (uOR) for SGA when using STOPPAM (SGASTOPPAM) and Intergrowth-21 (SGAIG21) references. Panels C and D shows the adjusted odds ratio (aOR).

Additional file 6: Figure S5. Funnel plots for meta-analysis. The closed dots indicate the observed studies, panel A indicate the trim and fill funnel plot for STOPPAM (P = 0.10) and panel B for Intergrowth-21 reference (P = 0.35). The contour enhanced funnel plot for STOPPAM (panel C) and Intergrowth-21 (panel D) show the distribution of studies in both the small and large p-values contours, hence no publication bias.

Objectives. Heart transplantation (HTx) has become an established treatment option in patients with end-stage heart failure. The aim of this study was to report on long-term outcome over the past three decades. Design. Consecutive adult patients receiving first-time and isolated HTx from October 3, 1990, to November 2, 2020, at Rigshospitalet, Copenhagen, Denmark, were retrospectively evaluated. Data were obtained from the Scandinavian Transplant Registry and patient medical records. Recipients were grouped by time of transplantation...

Additional file 1: Fig. 1. Predicted probability of death across the 6 hypothermia groups as compared to the normothermia groups at the corresponding sites. Patients in group 1, with the lowest average temperature at 4 hours had a higher probability of death.

Additional file 3: Figure S2. The birth weight z-scores comparing STOPPAM vs. Intergrowth (IG21) references. The dash line indicates the mean birthweight z-scores.

Additional file 2: Figure S2. Effects of manipulating androgen production in ex vivo cultured human fetal testis tissue on expression of the Sertoli cell marker SOX9. Expression pattern of the Sertoli cell marker SOX9 in fetal testis samples treated with ketoconazole (10-6 M) and hCG (1 IU/ml) for two weeks in ex vivo culture. Images representative for the expression in each age-group and treatment. Counterstaining with Mayer haematoxylin, scale bar corresponds to 50 µm.

Additional file 3: Figure S3. Effects of reduced androgen exposure via blocking of the androgen receptor in ex vivo culture of human fetal testes. Quantification of A) testosterone B) androstenedione and C) DHEAS secretion by ex vivo cultured fetal testis tissue from GW 7-12 following treatment with flutamide (10-6 M) and ketoconazole (10-6 M) for 14 days. Media were collected every 48 hours throughout the 14-day culture period and were pooled for each individual tissue...