Additional file 1: Supplementary Figure 1. MMP-13 levels in the medium of IL-1β-induced C28/I2 cells detected by ELISA.

Additional file 1: Figure S1.Expression, purification and immunoblot analysis of recombinant PvRAMA protein. A Expression and purification of recombinant PvRAMA (appox. 43 kDa). M, Marker; T, total translation mix; S, supernatant; P, precipitate; Ft, flow through; Ne, elution treated with non-reducing buffer; E, elution treated with reducing buffer. b-d Recombinant PvRAMA protein (appox. 43 kDa) under reducing conditions was probed with the anti-His tag antibody (b), rPvRAMA-immunized mouse serum (c) and PBS-immunized mouse serum (d).

Additional file 3: Figure S2.Comparison of PvRAMA amino acid sequences. The first line was the reference sequence: PvRAMA of the Sal-1 strain. The number of P. vivaxisolates with the same amino acid sequence is shown in parentheses.

Additional file 7: Fig. S7. Transcription and DNA methylation Profile of Each Cell Lines, Related to Fig. 6. (A) Unsupervised hierarchical clustering of the transcriptomes (two independent experiments) of each sample, AU (Approximately Unbiased) p-value and BP (Bootstrap Probability) value; (B) Hierarchical clustering on the top changed 1000 promoters by RnBeads; (C) Heat map of key PGC-associated genes (early and late), pluripotency, mesoderm, endoderm, and gonadal somatic (Soma) markers; (D) Violin plots showing distribution of...

Supplemental material, sj-tif-3-onc-10.1177_11795549221134735 for First-Line Osimertinib in Patients With EGFR-Mutated Non-Small Cell Lung Cancer: Effectiveness, Resistance Mechanisms, and Prognosis of Different Subsequent Treatments by Naifu Nie, Jianghua Li, Jian Zhang, Jie Dai, Zhulin Liu, Zhenyu Ding, Yubo Wang, Mengxiao Zhu, Chen Hu, Rui Han, Huan Tang, Li Li and Yong He in Clinical Medicine Insights: Oncology

Additional file 4: Figure S2. Hazard ratios for long-term all-cause mortality in different subgroups.

Additional file 1: Fig. S1. (A) Kaplan-Meier survival analysis of ten hub genes (KNG1, CYP11A1, SMPD1, DAND5, NKPD1, RP11-59D5_B.2, CTD-2184C24.2, RP11-680F8.3, RP11-51F16.9, CTD-2012K14.8) in L_cancer patients between high-expression and low-expression groups. (B) Kaplan-Meier survival analysis of ten hub genes (MOCS1, PTGS2, PLEKHA8P1, ZC3H12C, LPO, METTL11B, RP11-278A23.1, RP11-452K12.7, RP11-742B18.1, RP11-626H12.2) in R_cancer patients between high-expression and low-expression groups.

Additional file 11: Supplementary Figure S11. Expression of immune checkpoints in the low and high-risk groups for CESC (A), OV (B), UCEC (C), and UCS D. ∗P<0.05; ∗∗P<0.01; ∗∗∗P<0.001; ns: not significant. The blue bars represent the low-risk group and the red bars represent the high-risk group.

Additional file 1: Fig S1. Validation of the hub genes by ROC analysis. (A) ROC analysis of the SS cohort (GSE89632); (B) was ROC analysis of the NASH cohort (GSE89632); (C) was ROC analysis of the COVID-19 cohort (GSE147507). ROC, receiver operating characteristic; SS, simple steatosis; NASH, nonalcoholic steatohepatitis; COVID-19, coronavirus disease-2019.

Supplemental material, sj-tiff-2-tah-10.1177_20406207221146031 for Antihuman T lymphocyte porcine immunoglobulin combined with cyclosporine as first-line immunosuppressive therapy for severe aplastic anemia in China: a large single-center, 10-year retrospective study by Wenrui Yang, Xu Liu, Xin Zhao, Li Zhang, Guangxin Peng, Lei Ye, Kang Zhou, Yuan Li, Jianping Li, Huihui Fan, Yang Yang, Youzhen Xiong, Liping Jing and Fengkui Zhang in Therapeutic Advances in Hematology

Supplemental material, sj-tiff-3-tah-10.1177_20406207221146031 for Antihuman T lymphocyte porcine immunoglobulin combined with cyclosporine as first-line immunosuppressive therapy for severe aplastic anemia in China: a large single-center, 10-year retrospective study by Wenrui Yang, Xu Liu, Xin Zhao, Li Zhang, Guangxin Peng, Lei Ye, Kang Zhou, Yuan Li, Jianping Li, Huihui Fan, Yang Yang, Youzhen Xiong, Liping Jing and Fengkui Zhang in Therapeutic Advances in Hematology

Supplemental Material for Fiber reinforced polypropylene composites interfacial behavior improvement fabricated by cold plasma jet SiOx nanoparticles deposition by Lin Zhang, Zhangchuan Xia, Yadong He, Chunling Xin, Yang Yu, Feng Ren and Ruixue Wang in Journal of Composite Materials

Supplemental Material for Fiber reinforced polypropylene composites interfacial behavior improvement fabricated by cold plasma jet SiOx nanoparticles deposition by Lin Zhang, Zhangchuan Xia, Yadong He, Chunling Xin, Yang Yu, Feng Ren and Ruixue Wang in Journal of Composite Materials

Hepatitis E virus (HEV) infection is primarily manifesting as acute hepatitis, but extra-hepatic replication and injury are frequently reported. During the study period, we discovered two acute myeloid leukaemia (AML) patients infected with HEV genotype 3 and 4, respectively, and HEV RNA and/or viral proteins were persistently detected in the bone marrow of both patients. The finding suggests that HEV can replicate in human bone marrow as it may serve as a new target site...

The sodium channel NaV1.5, which is encoded by the SCN5A gene, underlies the fast upstroke of cardiac action potential and thus plays a crucial role in cardiac electrophysiology, but the mechanism governing the regulation of NaV1.5 has not been fully elucidated. The newly developed clustered regularly interspaced short palindromic repeats (CRSPR)/Cas9 transcription factors offer a powerful and precise approach for modulating gene expression. We investigated the potential of this new tool for activating stringently silenced...

Additional file 6: Figure S2. miRNAs with similar expression patterns in crown and leaf tissues at the winter dormancy stage, spring green-up stage and jointing stage.

Additional file 7: Figure S3. miRNAs with different expression patterns in crown and leaf tissues at the winter dormancy stage, spring green-up stage and jointing stage.

Additional file 2: Figure S1. Common and specific known miRNAs expressed in wheat crown tissues at four different growth and development stages.

The dysregulated circular RNAs (circRNAs) are linked to progression and chemoresistance in colorectal cancer (CRC). However, the role of circRNA protein tyrosine kinase 2 (circPTK2) in CRC progression and chemoresistance is uncertain. The circPTK2, microRNA (miR)-136-5p, m6A ‘reader’ protein YTH domain family protein 1 (YTHDF1), β-catenin and cyclin D1 abundances were examined via quantitative reverse transcription PCR or Western blotting. The progression was investigated by cell counting kit-8 (CCK-8), colony formation, transwell and xenograft analysis....

Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

Triggering receptor expressed on myeloid cells 1 (TREM1) participates in the development of endometritis. This study aims at identifying the effects and interaction of TREM1 and upstream stimulatory factor 2 (USF2) in endometritis by using a model of lipopolysaccharide (LPS)-induced human endometrial epithelial cells (HEnEpCs). ELISA was performed to determine the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF-α) after LPS stimulation. TREM1 and USF2 expression was examined with RT-qPCR and Western blot....

Supplemental material, sj-jpg-3-tej-10.1177_20417314221147113 for Construction of a pancreatic cancer nerve invasion system using brain and pancreatic cancer organoids by Chenyun Song, Xinyu Chen, Jixin Ma, Hada Buhe, Yang Liu, Hexige Saiyin and Lixiang Ma in Journal of Tissue Engineering

Supplementary figure 1-3 Supplementary table 1-2

Hmg-CoA Reductase (Hmgcr) catalyzes the conversion of Hmg-CoA to mevalonate, which is the rate-limiting step in the cholesterol biosynthetic pathway. Hmgcr is the target of statins, which are the front line therapy for hypercholesterolemia. In addition to cholesterol, Hmgcr activity is also required for the synthesis of non-sterol isoprenoids, such as heme A, dolichol, ubiquinone, farnesylated and geranylgeranylated proteins. The goal of this project is to investigate the effects of Hmgcr inhibition on non-sterol isoprenoids...

Hmg-CoA Reductase (Hmgcr) catalyzes the conversion of Hmg-CoA to mevalonate, which is the rate-limiting step in the cholesterol biosynthetic pathway. Hmgcr is the target of statins, which are the front line therapy for hypercholesterolemia. In addition to cholesterol, Hmgcr activity is also required for the synthesis of non-sterol isoprenoids, such as heme A, dolichol, ubiquinone, farnesylated and geranylgeranylated proteins. The goal of this project is to investigate the effects of Hmgcr inhibition on non-sterol isoprenoids...