Additional file 7: Figure S7. Effect of LPS and CD40L on TRAFs proteins in EGCs. a Representative western blot images of TRAFs proteins expression in EGCs. b–g Western blot analysis of TRAF1 (b), TRAF2 (c), TRAF3 (d), TRAF4 (e), TRAF5 (f) and TRAF6 (g) expression in different groups of EGCs (n = 6). The data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Additional file 1: Figure S1. Transfection efficiency of RNAs. A. SOX21-AS1 expression was reduced in PC cells via transfecting shRNAs targeting SOX21-AS1. B IF assays detected the intensity of EMT markers in sh-SOX21-AS1 transfected PC cells. C SOX21 expression was elevated in PC cells by pcDNA-SOX21 transfection. D MiR-576-5p expression was elevated by miR-576-5p mimics transfection. **P < 0.01.

Supplementary Figure 3. The YY2 expression after miR-1208 mimic or inhibitor treatment in GSCs.a, b: qPCR assays were performed to detect the expression of YY2 in GSC15 after miR-1208 mimic or in GSC17 after miR-1208 inhibitor treatment. c, d: Western blotting showed the expression of YY2 in GSC15 after miR-1208 mimic or in GSC17 after miR-1208 inhibitor treatment. All data are shown as the mean ? SD (three independent experiments). *P < 0.05; **P <...

Additional file 3. Collection Proof of Strain GDFS1009.

Supplementary Figure 1. The validation of the patient-derived GSCs from fresh clinical GBM specimens. a: Immunofluorescence staining of the stem cell markers CD133+ and Nestin+ in the isolated neurospheres. b: The multi-lineage differentiation capacity and differentiated markers GFAP and ?IIItubulin were shown in the isolated neurospheres. c: The expression of circPTPRF in six GSCs and normal human astrocyte cells (NHA) was detected by qPCR. All data are shown as the mean ? SD (three independent...

Supplementary Material 1

Supplementary Material 1

Additional file 4: Figure S3. Volcano plot showing the log2 fold change of 517 significantly differentially expressed miRNAs in CRC patients from the TCGA database.

Additional file 5: Figure S4. Prediction of 6 miRNAs (miR-29b-3p, miR-4677-3p, miR-144-3p, miR-30b-5p, miR-30e-5p and miR-374b-5p) targeting LIFR-AS1 in CRC.

Additional file 1: Supplementary Figure 1. Kaplan-Meiercurves of OS differences stratified by age, gender, tumor grade and stagebetween both groups in the entire TCGA set.

Additional file 2: Supplementary Figure 2. Partial sensitive compounds.

Additional file 1: Fig. S1. (A) Kaplan-Meier survival analysis of ten hub genes (KNG1, CYP11A1, SMPD1, DAND5, NKPD1, RP11-59D5_B.2, CTD-2184C24.2, RP11-680F8.3, RP11-51F16.9, CTD-2012K14.8) in L_cancer patients between high-expression and low-expression groups. (B) Kaplan-Meier survival analysis of ten hub genes (MOCS1, PTGS2, PLEKHA8P1, ZC3H12C, LPO, METTL11B, RP11-278A23.1, RP11-452K12.7, RP11-742B18.1, RP11-626H12.2) in R_cancer patients between high-expression and low-expression groups.

Additional file 1: SupplementaryFigure 1. The top 10 cited countrys related to this field.

Recently, the rs41291957 polymorphism in the promoter region of miR-143/145 has been repeatedly investigated for its contribution to cancer susceptibility. However, the results remain conflicting rather than conclusive, which calls for further investigations. Therefore, we here conducted a case-control study and meta-analysis to explore the association between rs41291957 and cancer risk. In the case-control study, a total of 2277 cancer patients (lung, liver, gastric and colorectal cancers) and 800 normal controls were recruited, the genotyping...

Additional file 1: Figure S1. WB raw data of Figure 2A.

Non-small-cell lung cancer patients harboring TP53/KRAS co-mutation could benefit from a PD-L1 inhibitor Supplementary Figure 4

Non-small-cell lung cancer patients harboring TP53/KRAS co-mutation could benefit from a PD-L1 inhibitor Supplementary Figure 4

Additional file 2: Fig. S1. A Schematic diagram of the genomic locus of CEBPA-DT (www.ncbi.nlm.nih.gov/). B The protein-coding potential of CEBPA-DT predicted by Open reading frame (ORF) Finder software prediction ( https://www.ncbi.nlm.nih.gov/orffinder/ ). C The protein-coding potential of CEBPA-DT predicted by Coding-Potential Assessment Tool ( wlcb.oit.uci.edu/cpat/ ).

Additional file 5: Fig. S4. A Clustering heatmap of significant differentially expressed mRNAs in HCCLM9 cells transfected with negative control or CEBPA-DT siRNAs. B Significantly enriched pathways annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Significantly enriched pathways annotated by the Gene Ontology (GO) database. D Significantly enriched pathways annotated by the Molecular Signatures Database (MSigDB). E The binding sites of hnRNPC and 3’UTR of DDR2 were predicted by PRIdictor database...

Additional file 6: Fig. S5. A-B The expression levels of DDR2 were measured by western blot and RT-qPCR in indicated HCCLM9 and SNU-449 cells transfected with negative control siRNA or siRNAs targeting DDR2. C-D Cell migration capacity of indicated HCCLM9 and SNU-449 cells were measured by scratch wound-healing assays. Scale bar, 100μm. E-F Cell migration and invasion capacity of indicated HCCLM9 and SNU-449 cells were measured by transwell migration and matrigel invasion assays. Scale bar,...

Supplemental material, sj-tif-4-vmj-10.1177_1358863X221132591 for Circular RNA suppression of vascular smooth muscle apoptosis through the miR-545-3p/CKAP4 axis during abdominal aortic aneurysm formation by Jiabo Wei, Hui Wang and Qinming Zhao in Vascular Medicine

Astragalus polysaccharin (APS), an extract of Astragalus propinquus Schischk, exerts antitumor effects in hepatocellular carcinoma (HCC). This study investigated the mechanism of action of APS in HCC. Tumour-associated macrophages (TAMs) were treated with APS (0, 8, 16 mg/mL) for 24 h. APS (16 mg/mL)-treated TAMs were co-cultured with MHCC97H/Huh7 cells for 24 h. Finally, BALB/c nude mice were divided into PBS, APS (50 mg/kg), APS (100 mg/kg), APS (200 mg/kg) groups: mice were inoculated with...

The comprehensive pathological peculiarities of Neferine (NEF) have been testified in disparate diseases. But, the functions of NEF in choriocarcinoma progression remain unexplored. The research endeavoured to uncover the anti-tumour action of NEF in choriocarcinoma cells. NEF at diverse doses was employed to dispose JEG-3 and HTR-8 cells, and cell viability assessment adopted CCK-8 assay. After 60 μg/mL NEF management, BrdU-positive cells, apoptosis, migration, invasion and correlative factors were assessed. CHRF expression in choriocarcinoma tumour...

We, the Editors and Publisher of the journal Artificial Cells, Nanomedicine, and Biotechnology, have retracted the following article: Gang Wang, Ping Wang, Xiaojun Yan & Jing Liu (2019) Neferine hinders choriocarcinoma cell proliferation, migration and invasion through repression of long noncoding RNA-CHRF, Artificial Cells, Nanomedicine, and Biotechnology, 47:1, 4089–4096, DOI: 10.1080/21691401.2019.1671429 Since publication, concerns have been raised about the integrity of the data in the article. When approached for an explanation, the authors have been...

Chronic, subclinical inflammation was often observed in the diabetic wound area, causing inadequate and delayed wound-healing effects by failing to initiate cell migration, proliferation, and extracellular matrix deposition. Therefore, we presented macrophage-derived exosomes (Exos) and explored their potential for inhibiting inflammation and accelerating diabetic wound healing in a skin defect, diabetic rat model. A thorough investigation demonstrated that Exos exerted anti-inflammatory effects by inhibiting the secretion of pro-inflammatory enzymes and cytokines. Furthermore, they accelerated the...