Transarterial chemoembolization is a standard treatment for intermediate-stage hepatocellular carcinoma (HCC). This study evaluated the anti-tumor effect of the semi-interpenetrating network (IPN) hydrogel as a novel embolic material for trans-portal vein chemoembolization (TPVE) in vivo. A nude mice orthotopic HCC model was established, followed by TPVE using IPN hydrogel loaded with or without cisplatin. Portal vein blockade was visualized by MRI and the development of tumor was monitored by IVIS Spectrum Imaging. Tumor proliferation and...

Supplemental material, sj-tif-4-vmj-10.1177_1358863X221132591 for Circular RNA suppression of vascular smooth muscle apoptosis through the miR-545-3p/CKAP4 axis during abdominal aortic aneurysm formation by Jiabo Wei, Hui Wang and Qinming Zhao in Vascular Medicine

Supplemental material, sj-tif-5-vmj-10.1177_1358863X221132591 for Circular RNA suppression of vascular smooth muscle apoptosis through the miR-545-3p/CKAP4 axis during abdominal aortic aneurysm formation by Jiabo Wei, Hui Wang and Qinming Zhao in Vascular Medicine

Head and neck squamous cell carcinoma (HNSCC) remains a dreadful malignancy bearing poor clinical efficacy, with emerging evidences indicating RNA-binding proteins’ (RBPs’) relevance to the evolution of the disease. Categorized as RBPs, the K-homology domain-containing 1 (KHDC1) family is proved to be closely related to cell survival and death. As a novel KHDC1 member, only one study is currently available in osteoarthritis synovial cells to unveil KHDC1L’s function of promoting proliferation. Nevertheless, to the best...

Aim: Diagnostic methods for HSV-1 need to be updated. Materials & methods: Serial dilutions of HSV- 1 DNA and ocular pathogenic microorganisms were tested by loop-mediated isothermal amplificationlateral flow dipstick assay (LAMP-LFD assay). The reliability of the LAMP-LFD assay and quantitative realtime PCR (qPCR) for HSV-1 detection was also validated using 30 clinical swab samples. Results: The LAMP reaction is optimal at 65◦C and 30 min. And the LAMP-LFD assay could detect HSV-1 up to...

Additional file 3: Fig. S2. A The expression levels of CEBPA-DT in different hepatoma cell lines measured by RT-qPCR. B Subcellular location of CEBPA-DT in indicated Huh7 cells examined by subcellular RNA fractionations and RT-qPCR analysis, β-actin and U3 were used as cytoplasmic and nuclear endogenous control, respectively. C The expression levels of CEBPA-DT in HCCLM9 and SNU-449 cells transfected with control siRNA or siRNA against CEBPA-DT. D qPCR quantification of the expression levels of...

Additional file 5: Fig. S4. A Clustering heatmap of significant differentially expressed mRNAs in HCCLM9 cells transfected with negative control or CEBPA-DT siRNAs. B Significantly enriched pathways annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Significantly enriched pathways annotated by the Gene Ontology (GO) database. D Significantly enriched pathways annotated by the Molecular Signatures Database (MSigDB). E The binding sites of hnRNPC and 3’UTR of DDR2 were predicted by PRIdictor database...

Additional file 7: Fig. S6. A-B Scratch wound-healing assays showed cell migration capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. C-D Transwell migration and matrigel invasion assays showed cell migration and invasion capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. E Representative images (fluorescence) of lung metastatic...

Supplemental material, sj-tif-1-tct-10.1177_15330338231154092 for Genetic and Clinical Characteristics of Patients with Philadelphia-Negative Myeloproliferative Neoplasm Carrying Concurrent Mutations in JAK2V617F, CALR, and MPL by Yan Wang, Fei Ran, Jin Lin, Jing Zhang and Dan Ma in Technology in Cancer Research & Treatment

The files include supplementary tables and detailed methods used in the manuscript, such as human acetabular cartilage dissociation and nuclei isolation and library construction, and 4 supplementary figures.

The files include supplementary tables and detailed methods used in the manuscript, such as human acetabular cartilage dissociation and nuclei isolation and library construction, and 4 supplementary figures.

Additional file 2: Figures S1. Confirmation of exosomes by transmission electron microscopy (TEM).

Additional file 2: Figures S1. Confirmation of exosomes by transmission electron microscopy (TEM).

Additional file 3: Fig. S3. Show the correlation of L_cancer RiskScore and L_cancer hub genes expression with immune infiltration level in L_cancer patients.

Non-small-cell lung cancer patients harboring TP53/KRAS co-mutation could benefit from a PD-L1 inhibitor Supplementary Figure 2

Supplemental material, sj-png-1-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Additional file 2. Supplement Figure.

Additional file 2: Fig. S1. Effects of circGSAP on the proliferation, migration and mortality of PMECs under normoxia. A Expression levels of circGSAP in PMECs treated with circGSAP under normoxia (n = 3). B-D Cell proliferation analysis, wound healing analysis and cell migration analysis of PMECs overexpressing circGSAP under normoxia (n = 5 or 4). E Cell mortality analysis of PMECs overexpressing circGSAP under normoxia (n = 4). All data are presented as the mean...

Supplemental material, sj-tif-1-car-10.1177_19476035221142011 for Ferroptosis Plays a Role in Human Chondrocyte of Osteoarthritis Induced by IL-1β In Vitro by Wenbo Xu, Bowen Zhang, Chunyang Xi, Yong Qin, Xin Lin, Bo Wang, Pengyu Kong and Jinglong Yan in CARTILAGE

Additional file 2: Fig. S1. JAK1 overexpression suppresses EC cell growth and migration.

Additional file 3: Fig. S2. JAK1 knockdown elevates the expression of HIF downstream genes in SPEC-2 cells.

Additional file 4: Fig. S3. JAK1 overexpression downregulates the expression of HIF downstream genes in Ishikawa cells.

Supplementary Material 2

Additional file 2. Collection Proof of Strain CM100Z4.

Additional file 4. Collection Proof of Strain SBW10264.