2 Works

Stepwise-edited, human melanoma models reveal mutations’ effect on tumor and microenvironment

Eran Hodis & Elena Torlai Triglia
Establishing causal relationships between genetic alterations of human cancers and the specific phenotypes of malignancy remains a challenge. We sequentially introduced mutations into healthy human melanocytes in up to five genes spanning six commonly disrupted melanoma pathways forming nine genetically distinct cellular models of melanoma. We connected mutant melanocyte genotypes to malignant cell expression programs in vitro and in vivo, replicative immortality, malignancy, rapid tumor growth, pigmentation, metastasis, and histopathology. Mutations in malignant cells also...

Quantification of Bone Marrow Compartments in Histological Sections

Josefine Tratwal & Olaia Naveiras
We report the development of a semi-automated image analysis plug-in, MarrowQuant, which employs the open-source software QuPath, to systematically quantify multiple bone components in H&E sections in an unbiased manner. MarrowQuant discerns and quantifies the areas occupied by bone, adipocyte ghosts, hematopoietic cells and the interstitial/microvascular compartment. A separate feature, AdipoQuant, fragments adipocyte ghosts in H&E-stained sections of extramedullary adipose tissue sections to render adipocyte area and size distribution. Quantification of BM hematopoietic cellularity with...

Registration Year

  • 2022
    1
  • 2021
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Resource Types

  • Image
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Affiliations

  • Harvard University
    2
  • Hospital Universitario 12 De Octubre
    1
  • École Polytechnique Fédérale de Lausanne
    1
  • Washington University Medical Center
    1
  • Bar-Ilan University
    1
  • Brigham and Women's Hospital
    1
  • Broad Institute
    1
  • University Hospital of Lausanne
    1