Staphylococcus aureus (S. aureus), especially methicillin-resistant Staphylococcus aureus (MRSA), is a major cause of pneumonia, resulting in severe morbidity and mortality in adults and children. Sortase A (SrtA), which mediates the anchoring of cell surface proteins in the cell wall, is an important virulence factor of S. aureus. Here, we found that salvianolic acid A (Sal A), which is a natural product that does not affect the growth of S. aureus, could inhibit SrtA activity...

Staphylococcus aureus (S. aureus), especially methicillin-resistant Staphylococcus aureus (MRSA), is a major cause of pneumonia, resulting in severe morbidity and mortality in adults and children. Sortase A (SrtA), which mediates the anchoring of cell surface proteins in the cell wall, is an important virulence factor of S. aureus. Here, we found that salvianolic acid A (Sal A), which is a natural product that does not affect the growth of S. aureus, could inhibit SrtA activity...

To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Additional file 2: Figure S2. Results of western blot analysis showed that ETV2 overexpression induced the the activation of p-ERK and p-AKT at days 3, 7 and 14 of osteogenic differentiation. *P < 0.05, compared with the 0 day group.

Additional file 3: Figure S3. HE (scale bar = 200 μm) and Masson (scale bar = 50 μm) staining in the rat calvarial defect model (n = 6).

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Additional file 2: Figure S1. Differential expression of the 11 genes between normal and MM tissues in the (A) GSE6477 and the (B) GSE118985 datasets.

Additional file 4: Figure S3. The relationship between clinical characteristics and risk scores in the training cohort.

Additional file 4: Figure S3. The relationship between clinical characteristics and risk scores in the training cohort.

Additional file 5: Figure S4. Changes in RAS signaling pathway (A and B) and intracellular ATP level (C and D) after drug(s) treatment by invitro experiments.

Additional file 1: Fig. S1. The mRNA levels of candidate genes in different molecular subtypes of breast cancer. ER/PR+ (ER/PR+ and HER−, n = 685), HER2+ (n = 168), and TNBC (n = 177). TCGA BRCA ER, PR, and HER2 status were retrieved from Thennavan et al. [21].

Additional file 4: Figure S4. Age-standardized DALYs rate at the global, regional, and national level. (A) Age-standardized DALYs rate of early-onset colorectal cancer globally and for 21 GBD regions by SDI, 1990–2019. (B) Age-standardized DALYs rates of early-onset colorectal cancer for 204 countries and territories in 2019. The black line represents the expected age-standardized DALYs rate of rheumatic heart disease based solely on SDI. GBD: Global Burden of Diseases, Injuries, and Risk Factors Study; SDI:...

Additional file 5: Figure S5. Percentage of age-standardised DALYs due to early-onset colorectal cancer attributable to risk factors for 21 GBD regions, both sexes, 2019. GBD: Global Burden of Diseases, Injuries, and Risk Factors Study; SDI: Socio-demographic index; DALYs, Disability-Adjusted Life Years.

Additional file 3: Figure S1. (A, D, G) The heat map of 10 prognostic DEOSG expressions in the train (A), testing (D), and entire sets (G), respectively. (B, E, H) The comparison of 10 prognostic DEOSG expressions between high- and low-risk groups in the training (B), testing (E), and entire sets (H), respectively. (C, F, I) The correlations among 10 prognostic DEOSG between high- and low-risk groups in the training (C), testing (F), and entire...

Additional file 3: Fig. S2. A The expression levels of CEBPA-DT in different hepatoma cell lines measured by RT-qPCR. B Subcellular location of CEBPA-DT in indicated Huh7 cells examined by subcellular RNA fractionations and RT-qPCR analysis, β-actin and U3 were used as cytoplasmic and nuclear endogenous control, respectively. C The expression levels of CEBPA-DT in HCCLM9 and SNU-449 cells transfected with control siRNA or siRNA against CEBPA-DT. D qPCR quantification of the expression levels of...

Additional file 8: Fig. S7. A-B Subgroup analyses showed prognostic value of CEBPA-DT for overall survival (top) and recurrence-free survival (bottom) in HCC patients with differential features.

Additional file 8: Fig. S7. A-B Subgroup analyses showed prognostic value of CEBPA-DT for overall survival (top) and recurrence-free survival (bottom) in HCC patients with differential features.

Additional file 7: Fig. S7. Mapchart shown the distribution of GRAS genes on 7 chromosomes in Chinese rosa.

Additional file 3: Fig. S2. Multicollinearity analysis for BMI and the MNA-SF.

Low meat performance is the defect of Small Tail Han sheep. Intramuscular fat affects meat quality and largely determined by adipogenesis. In previous study, miR136 was showed one of differentially expressed microRNAs between preadipocytes and mature adipocytes of Small Tail Han sheep but its role in adipogenesis is still not elucidated. Here, we investigated the effect of miR136 on adipogenesis and the underlying mechanism. qPCR data showed that miR136 level increased with preadipocytes proliferation while...

Common bunt disease of wheat in China is caused mainly by Tilletia foetida. However, reliable approaches for determining disease-associated biochemical markers are rarely reported. Here, a headspace-solid-phase microextraction coupled with headspace GC-tandem mass spectrometry (HS-SPME-GC-MS) was used to screen volatile substances in infected wheat, and an optimal chemical marker was selected to establish analytical methods for disease determination. Non-targeted screening of 13 volatile compounds unique to diseased wheat allowed a metabolite with rotten fish-like smell,...

Common bunt disease of wheat in China is caused mainly by Tilletia foetida. However, reliable approaches for determining disease-associated biochemical markers are rarely reported. Here, a headspace-solid-phase microextraction coupled with headspace GC-tandem mass spectrometry (HS-SPME-GC-MS) was used to screen volatile substances in infected wheat, and an optimal chemical marker was selected to establish analytical methods for disease determination. Non-targeted screening of 13 volatile compounds unique to diseased wheat allowed a metabolite with rotten fish-like smell,...

Human papillomavirus (HPV) infection and related diseases are clinical challenges. The efficacy of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) using red laser (630 ± 5 nm) is remarkable and safe. In this study, we aim to investigate the efficacy of ALA-450 nm PDT comparing with ALA-635 nm PDT. We detected cell proliferation and cell apoptosis through MTT assay and flow cytometry assay respectively. Flow cytometry assay determined the intracellular reactive oxygen species (ROS) generation. Western blotting...

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