LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

Additional file 3: Figure S3. ShRNA Panx 1-AAV or Ctrl-AAV-labeled EGFP-positive cells have a sound co-localization with S100 β-labeled Schwann cells. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 50 μm. Lower panel, scale bar = 25 μm. n = 3–4 mice/group.

Additional file 7: Figure S7. Cytokine array reveals Panx 1-dependent cytokines and chemokines in LPS-treated Schwann cells. A. Coordinates of the cytokine array containing 40 different cytokines and chemokines, including three positive control (PC) proteins. B. Array membranes of protein expression among Ctrl, Ctrl-siRNA, and Panx 1-siRNA groups in LPS-treated Schwann cells.

Additional file 1: Fig. S1. A The process of miR-1268a derived from OL-SCs cells entering OL cells through exosomes (Cy5 marks miR-1268a, PKH26 marks exosomes, and DiO marks cell membrane). Scale bar, 10 nm. B, C Graphs of the results of the remaining two replicate experiments in the cell cycle. D The proliferation ability of OL cells transfected with mir-1268a mimics and NC mimics was detected by EdU analysis, p > 0.05. E The spheroidization...

Additional file 1: Fig. S1. A The process of miR-1268a derived from OL-SCs cells entering OL cells through exosomes (Cy5 marks miR-1268a, PKH26 marks exosomes, and DiO marks cell membrane). Scale bar, 10 nm. B, C Graphs of the results of the remaining two replicate experiments in the cell cycle. D The proliferation ability of OL cells transfected with mir-1268a mimics and NC mimics was detected by EdU analysis, p > 0.05. E The spheroidization...

Additional file 15: Figure S6. circPVT1 promotes the migration and invasion of NPC cells by regulating cell adhesion andcytoskeleton remodeling. The 231 differentially expressed proteins regulated by c-Myc from LC-MS/MS data using the Ingenuity Pathway Analysis (IPA) software.

Additional file 10: Supplementary Figure S10. Spearman correlation coefficient between 22 immune cells was calculated for CESC (A), OV (B), UCEC (C), and UCS D. Red bubbles indicate positive correlations and blue bubbles indicate negative correlations. The numbers in the bubbles represent the correlation coefficients.

Additional file 1: Supplementary Figure S1. Mutation frequency and expression variation of the 76 necroptosis-related genes (NRGs). A-D Mutation frequency of NRGs in patients with CESC (A), OV (B), UCEC (C), and UCS D. The small figure above shows the TMB, the number on the right shows the mutation frequency of each NRG, and the figure on the right shows the proportion of each vari©. E Expression levels of NRGs in four gynecological tumors. The...

Additional file 3: Supplementary Figure S3. The survival curves of necroptosis-score. A-D Kaplan–Meier curve was used to analyze the survival rate of CESC (A), OV (B), UCEC (C), and UCS (D) patients with high or low necroptosis-score. Black curves indicate low necroptosis-score and red curves indicate high necroptosis-score.

Additional file 5: Supplementary Figure S5. The ROC curves for the risk model in the four GCs. A-C The ROC curves of CESC for training (A), validation (B), and total (C) sets. D-F The ROC curves of OV for training (D), valida©n (E), and total (F) sets. G-I The ROC curves of UCEC for training (G), validation (H), and total (I) sets. J-L The ROC curves of UCS for training (J), validation (K), and total...

Additional file 9: Supplementary Figure S9. The waterfall plot of somatic mutation features established with risk scores. A-B The waterfall plot of somatic mutation in CESC for high-risk group (A) and low-risk group B. C-D The waterfall plot of somatic mutation in OV for high-risk group (C) and low-risk group D. E-F The waterfall plot of somatic mutation in UCEC for high-risk g©p (E) and low-risk group F. G-H The waterfall plot of somatic mutation...

Additional file 7: Figure S2. Expression difference of ZNF71 across genders.

Additional file 8: Figure S3. Expression analysis of ZNF71’s putative target and tight junctions-related genes.

Additional file 4: Figure S2. Hazard ratios for long-term all-cause mortality in different subgroups.

Additional file 11: Supplementary Figure S11. Expression of immune checkpoints in the low and high-risk groups for CESC (A), OV (B), UCEC (C), and UCS D. ∗P<0.05; ∗∗P<0.01; ∗∗∗P<0.001; ns: not significant. The blue bars represent the low-risk group and the red bars represent the high-risk group.

Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin, psilocin and ibotenic acid, etc. The mushrooms containing hallucinogenic components are various, widely distributed and lack of standard to define, which made a great challenge to identification. Traditional identification methods, such as morphology and toxicology analysis, showed shortcomings in old or processed samples, while the DNA-based identification of hallucinogenic mushrooms would allow to identify these samples due to the stability...

Additional file 1: Supplementary Fig. 1. Co-expression intensity of prognostic m7G-related IncRNAs and their associated mRNAs.

Additional file 1: Figure S1. Sorting of CD4+ T cells in CD4+CD25+Foxp3+Treg cells and CD3+CD8− cells in CD3+CD8−IL-17+Th17 cells analyzed by flow cytometry. Fig. S1A is related to Fig. 1A; Fig. S1B is related to Fig. 2C; Fig. S1C is related to Fig. 4A.

Additional file 1: Figure S1. Sorting of CD4+ T cells in CD4+CD25+Foxp3+Treg cells and CD3+CD8− cells in CD3+CD8−IL-17+Th17 cells analyzed by flow cytometry. Fig. S1A is related to Fig. 1A; Fig. S1B is related to Fig. 2C; Fig. S1C is related to Fig. 4A.

Additional file 5: Fig. S5. Representative picture of HE staining for mouse ID8 OC. The section for tumors of nonvaccinated mice (a) and mice immunized with unmodified whole-cell ID8 vaccine (b), α2-3NA modified DWCTV (c), and α2-6NA modified DWCTV (d). Scale bars: 100 μm.

Supplementary Material 3

Efficacy and safety of nivolumab for advanced/recurrent non-small-cell lung cancer: an up-to-date meta-analysis of large-scale phase III randomized controlled trials Supplementary figure 1

Additional file 2: Figure S2. The distribution of Panx 1 in the DRG and within sciatic nerves. A. Double-immunofluorescence images of Panx 1 (green) and the sciatic nerve axonal marker, NF200 (red). (B). Images of Panx 1 immunostaining (red) and, Nissl staining (green) in the DRG; Scale bar = 50 μm. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 100 μm. Lower panel, scale bar...

Supplemental material, sj-tif-1-taj-10.1177_20406223221146938 for Eosinophilia attention, diagnosis, treatment, and awareness in physicians: a cross-sectional survey by Bigui Chen, Yu Fu, Zhufeng Wang, Qiuping Rong, Qingling Zhang, Jiaxing Xie, Xuetao Kong and Mei Jiang in Therapeutic Advances in Chronic Disease

Additional file 1: Figure S1. Panx 2 and Panx 3 mRNA expression after CCI. A-B. A qPCR assay was used to detect Panx 2 (A) and Panx 3 (B) mRNA levels at 4 and 14 days post-CCI. The data are mean ± SEM. n = 3 mice/group. **p < 0.01, ***p < 0.001, vs. sham injury. The data were analyzed by one-way ANOVA followed by Dunnett’s test.