Additional file 2: Figure S2. The distribution of Panx 1 in the DRG and within sciatic nerves. A. Double-immunofluorescence images of Panx 1 (green) and the sciatic nerve axonal marker, NF200 (red). (B). Images of Panx 1 immunostaining (red) and, Nissl staining (green) in the DRG; Scale bar = 50 μm. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 100 μm. Lower panel, scale bar...

Common bunt disease of wheat in China is caused mainly by Tilletia foetida. However, reliable approaches for determining disease-associated biochemical markers are rarely reported. Here, a headspace-solid-phase microextraction coupled with headspace GC-tandem mass spectrometry (HS-SPME-GC-MS) was used to screen volatile substances in infected wheat, and an optimal chemical marker was selected to establish analytical methods for disease determination. Non-targeted screening of 13 volatile compounds unique to diseased wheat allowed a metabolite with rotten fish-like smell,...

Common bunt disease of wheat in China is caused mainly by Tilletia foetida. However, reliable approaches for determining disease-associated biochemical markers are rarely reported. Here, a headspace-solid-phase microextraction coupled with headspace GC-tandem mass spectrometry (HS-SPME-GC-MS) was used to screen volatile substances in infected wheat, and an optimal chemical marker was selected to establish analytical methods for disease determination. Non-targeted screening of 13 volatile compounds unique to diseased wheat allowed a metabolite with rotten fish-like smell,...

Additional file 7: Figure S2. Expression difference of ZNF71 across genders.

Additional file 2: Supplementary Figure S2. Correlation analysis of the 76 NRGs for CESC (A), OV (B), UCEC (C), and UCS D. Red indicates a positive correlation; blue indicates a negative correlation.

Additional file 2: Supplementary Figure S2. Correlation analysis of the 76 NRGs for CESC (A), OV (B), UCEC (C), and UCS D. Red indicates a positive correlation; blue indicates a negative correlation.

Additional file 6: Supplementary Figure S6. Expression values of NRGs in prognostic signature of OV. A-D Expression values of NRGs in TCGA for BACH2 (A), MLKL (B), MYC (C), MYCN (D), and SIRT2 (E). F-J Expression values of NRGs in our cohort for BACH2 (F), MLKL (I), MYC (G), MYCN (H), and SIRT2 (J).

Additional file 7: Supplementary Figure S7. Clinical value of risk score by independent prognostic analysis. A-H The Univariate Cox regression analysis and Multivariate Cox regression analysis for CESC (A-B), OV (C-D), UCEC (E-F), and UCS G-H.

Additional file 4: Fig. S4. DCs maturation/activation and subsequently lymphocyte activation. The PBMCs (a), immature DCs (b), co-incubation of DCs and tumor cells (c), and co-culture of mature DCs and lymphocytes (d) were observed under a phase-contrast microscope. Scale bars: 25 μm.

Additional file 4: Supplementary Fig. 4. Overall survival analysis of six m&G-related IncRNAs in high and low ezpression groups.

Additional file 4: Figure S4. The responses of LPS-treated cultured Schwann cells. A-E. mRNA expression levels of the inflammatory factors, IL-1β, IL-6, TNF-α, and TGF-β (A-D), the apoptosis marker, caspase 3 (E) and in LPS-treated Schwann cells (0, 100, 500, and 1000 ng/ml, 24 h at 37℃). F. Cytomorphology of LPS-treated Schwann cells at different doses (0, 100, 500, and 1000 ng/ml) under a light microscope. All data are mean ± SEM. n = 3....

Additional file 6: Figure S6. CBX and Panx 1-siRNA do not affect TGF-β mRNA expression in LPS-treated Schwann cells. A-B. Pretreatment with CBX (100 mM) for 2 h or Panx 1 siRNA for 36 h, qPCR was used to determine TGF-β mRNA expression levels in LPS-treated Schwann cells. All data are mean ± SEM. n = 4.

Additional file 10: Figure S1. A. Thedifferentially expressed circRNAs between the high metastasis NPC cell line S18 and the low metastasis NPC cell line S26 (GSE137543, the RPM value ≥ 2and Fold changes ≥ 1.5). Circular RNA circPVT1 is highly expressed in NPC cell line S18. B. The Venn diagram of data sets GSE137543 and PRJNA391554. Circular RNA circPVT1 was identified for its high abundance in both sets of data.C. circPVT1 is mainly localized in...

Additional file 14: Figure S5. c-Myc is ubiquitinated substrate of β-TrCP. A. The expression of β-TrCP was examined in CNE2 and 5-8F cells after overexpression or knockdown of circPVT1 using western blotting.B. The β-TrCP binding proteins were identified in CNE2 cells by immunoprecipitation followed by LC-MS/MS.C. The interaction of c-Myc and β-TrCP was predicted by Molecular Docking ( http://hdock.phys.hust.edu.cn/ ). The blue part is the WD40 repeat domain of β-TrCP.D. The expression of c-Myc was...

Additional file 15: Figure S6. circPVT1 promotes the migration and invasion of NPC cells by regulating cell adhesion andcytoskeleton remodeling. The 231 differentially expressed proteins regulated by c-Myc from LC-MS/MS data using the Ingenuity Pathway Analysis (IPA) software.

Additional file12: Supplementary Figure S12. Spearman correlation between NRGs expression level and IC50 level of drugs. A Bubble chart for CESC. B Bubble chart for OV. C Bubble chart for UCEC. D Bubble chart for UCS. The bubble color indicates the degree of correlation index. The bubble size indicates the P-value. The correlation with P<0.05 & |Cor|>0.3 were retained to produce the figure.

Additional file 1: Figure S2. Sorting of CD4+ T cells in CD4+CD25+Foxp3+Treg cells and CD3+CD8− cells in CD3+CD8−IL-17+Th17 cells analyzed by flow cytometry. Fig. S2 is related to Fig. 5A.

Additional file 1: Fig. S1. Screening the mitomycin C concentration for OC cell treatment. Representative images under phase contrast microscopy of OC cells treated with 0, 50, and 100 ng ml-1 mitomycin C for 2 h (a) and 3 h (b) and cultured for 24 h. Scale bars represent 100 μm.

Additional file 1: Figure S1. The principal component analysis (PCA) plot for DAMs.

Additional file 1: Figure S1. The principal component analysis (PCA) plot for DAMs.

Additional file 3: Supplementary Fig. 3. The correlation between risk score and immune cell infiltration was analyzed. A Analysis of the correlation among 22 subtyopes of immune cells. B The TIMER data base was used to analyze the risk scores and six types of immune-infiltrating cells.

Osteoarthritis (OA) is a rheumatic disease common in the elderly. AGEs are the end products of glycation reactions and play an important role in the development of OA. Etomidate is a general anesthesia-inducing agent recently reported to exert significant anti-inflammatory effects. The present study aims to explore the protective effect of Etomidate against advanced glycation end-products (AGEs)-induced reduction of extracellular matrix gene expression in chondrocytes. In the present study, we found that AGEs significantly reduced...

The clinical significance and underlying molecular mechanism of miRNA-222-3p in metastatic prostate cancer (MPCa) remain unclear. The present study used a large number of cases (n = 1,502) based on miRNA chip and miRNA sequencing datasets to evaluate the expression and diagnostic potential of miRNA-222-3p in MPCa. We applied a variety of meta-analytic methods, including forest maps, sensitivity analysis, subgroup analysis and summary receiver operating characteristic curves, to prove the final results. MiRNA-222-3p was reduced...

Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides. One of the prerequisites of enzyme fermentation production is to select and breed enzyme producing cells with good performance. So in the process of fermentation production, the low yield of chitosanase cannot meet the current requirement. In this paper, a strain producing chitosanase was screened and identified, and a novel mutagenesis system (Atmospheric and Room Temperature Plasma (ARTP)) was selected...

Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides. One of the prerequisites of enzyme fermentation production is to select and breed enzyme-producing cells with good performance. So in the process of fermentation production, the low yield of chitosanase cannot meet the current requirement. In this paper, a strain producing chitosanase was screened and identified, and a novel mutagenesis system (Atmospheric and Room Temperature Plasma (ARTP)) was selected to...