Additional file 1: Figure S1. Panx 2 and Panx 3 mRNA expression after CCI. A-B. A qPCR assay was used to detect Panx 2 (A) and Panx 3 (B) mRNA levels at 4 and 14 days post-CCI. The data are mean ± SEM. n = 3 mice/group. **p < 0.01, ***p < 0.001, vs. sham injury. The data were analyzed by one-way ANOVA followed by Dunnett’s test.

Additional file 2: Fig. S2 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of maternal parent (P2).

Additional file 16: Figure S7. c-Myc promotes circPVT1 generation by recruiting SRSF1 to couple transcription to splicing. A. The luciferase reporter gene activity of the PVT1 promoter was analyzed in NPC cells after overexpression of c-Myc. Data were represented as mean ± SD. ***, p < 0.001. B. The binding of transcription factor c-Myc to the PVT1 promoter detected by ChIP assay. Three sites on the PVT1 promoter were identified as potential c-Myc binding sites...

Additional file 2.

Additional file 1: Figure S3. Propofol inhibited invasion and migration of lung cancer cells. A Effect of different doses (0.3 μg/mL, 3 μg/mL, 30 μg/mL) of propofol on lung cancer cell viability was measured by CCK-8 assay; B Cell invasion were detected using Transwell assay; C Cell migration was detected Transwell assay; D Positive rate of E-cadherin protein was detected by immunocytochemistry and the stronger red fluorescence meant the higher positive rate. All experiments were...

Additional file 2: Fig. S1. JAK1 overexpression suppresses EC cell growth and migration.

Additional file 3: Fig. S2. JAK1 knockdown elevates the expression of HIF downstream genes in SPEC-2 cells.

Additional file 4: Fig. S3. JAK1 overexpression downregulates the expression of HIF downstream genes in Ishikawa cells.

Supplementary Material 2

Additional file 1: Supplementary Fig. 1. Co-expression intensity of prognostic m7G-related IncRNAs and their associated mRNAs.

Additional file 2: Supplementary Fig. 2. RT-qPCR was used to detect the expression of m7G-related IncRNAs. *p<0.05, **p<0.01, and ***p<0.001.

Additional file 11: Supplementary Figure S11. Expression of immune checkpoints in the low and high-risk groups for CESC (A), OV (B), UCEC (C), and UCS D. ∗P<0.05; ∗∗P<0.01; ∗∗∗P<0.001; ns: not significant. The blue bars represent the low-risk group and the red bars represent the high-risk group.

Additional file 4: Supplementary Figure S4. Statistical analysis of the number of differential NRGs and prognostic NRGs of the four GCs. Red bars show up-regulated NRGs in cancer tissue. The green bars show NRGs that are downregulated in cancer tissue. The blue bars show the sum of the differential NRGs. Yellow bars indicate prognostic NRGs.

Additional file 6: Supplementary Figure S6. Expression values of NRGs in prognostic signature of OV. A-D Expression values of NRGs in TCGA for BACH2 (A), MLKL (B), MYC (C), MYCN (D), and SIRT2 (E). F-J Expression values of NRGs in our cohort for BACH2 (F), MLKL (I), MYC (G), MYCN (H), and SIRT2 (J).

Additional file 8: Supplementary Figure S8. The Nomogram model based on risk model and clinical features for GCs. A-B The Nomogram (A) and calibration curve (B) for CESC. C-D The Nomogram (C) and calibration curve (D) for OV. (E-F) The Nomo©m (E) and calibration curve (F) for UCEC. G-H The Nomogram (G) and calibration curve (H) for UCS.

Supplementary Material 1

Additional file 1: Fig. S1. Shannon curve showed that all samples were saturated.

Additional file 1: Fig. S1. Shannon curve showed that all samples were saturated.

Additional file 9: Supplementary Figure S9. The waterfall plot of somatic mutation features established with risk scores. A-B The waterfall plot of somatic mutation in CESC for high-risk group (A) and low-risk group B. C-D The waterfall plot of somatic mutation in OV for high-risk group (C) and low-risk group D. E-F The waterfall plot of somatic mutation in UCEC for high-risk g©p (E) and low-risk group F. G-H The waterfall plot of somatic mutation...

Additional file 3: Fig. S3. Representative confocal immunofluorescence images of OC cells. (a) and (b) showed the images of unmodified, α2-3NA modified, and α2-6NA modified ID8 cells stained by α-2,3Sia (MAL II, green), α-2,6Sia (SNA, green), and Gal/GalNAc (PNA, pink). Scale bars: 100 μm.

Additional file 3: Fig. S3. Representative confocal immunofluorescence images of OC cells. (a) and (b) showed the images of unmodified, α2-3NA modified, and α2-6NA modified ID8 cells stained by α-2,3Sia (MAL II, green), α-2,6Sia (SNA, green), and Gal/GalNAc (PNA, pink). Scale bars: 100 μm.

Additional file 4: Fig. S4. DCs maturation/activation and subsequently lymphocyte activation. The PBMCs (a), immature DCs (b), co-incubation of DCs and tumor cells (c), and co-culture of mature DCs and lymphocytes (d) were observed under a phase-contrast microscope. Scale bars: 25 μm.

LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

Additional file 3: Figure S3. ShRNA Panx 1-AAV or Ctrl-AAV-labeled EGFP-positive cells have a sound co-localization with S100 β-labeled Schwann cells. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 50 μm. Lower panel, scale bar = 25 μm. n = 3–4 mice/group.

Additional file 7: Figure S7. Cytokine array reveals Panx 1-dependent cytokines and chemokines in LPS-treated Schwann cells. A. Coordinates of the cytokine array containing 40 different cytokines and chemokines, including three positive control (PC) proteins. B. Array membranes of protein expression among Ctrl, Ctrl-siRNA, and Panx 1-siRNA groups in LPS-treated Schwann cells.