Additional file 5: Figure S4. CircPVRL3 inhibits the cell proliferation in PDAC cells. (A) EdU assay, (B) colony formation assay, and (C) CCK-8 assay were performed to detect the function of circPVRL3 which effected on the cell proliferation in PANC-1 and CFPAC-1 cells. Scale bar = 100 μm. *p < 0.05; **p < 0.01; ***p < 0.001.

Additional file 6: Fig. S6. The expression patterns of the candidate miRNAs in clinical iCCA and adjacent nontumor liver tissue samples from TCGA dataset. (A) Boxplots showing that miRNAs (miR-424-5p, -424-3p, -497-5p, -15b-5p, -15a-5p, -16-5p, -195-5p and miR-16-5p) in iCCA and nontumor liver tissue samples from TCGA dataset. ns, p ≥ 0.05. **p < 0.01; ***p < 0.001, using two-tailed unpaired Student’s t test. (B-C) Heatmap shows the association of COL12A1 with miRNAs (miR-424-5p, -424-3p,...

Supplementary Material 1

Supplementary Material 2

Supplementary Material 3

Additional file 2: Fig. S2. Depression of Bim induced by ExosLean confers effects on wound healing and immune-regulation. (A) Representative images of wound closure in the different treatment groups. (B) The relative wound area was calculated; (C and D) Immunofluorescence staining image showing the localization of CD68+/CD86+ M1 macrophages and statistical analysis of the proportion of M1 macrophages; (E and F) Immunofluorescence staining image showing the localization of CD68+/CD206+ M2 macrophages and statistical analysis of...

Supplementary Material 6

Additional file 3: Fig. S3. Quantification of Alizarin Red S staining in Figure 2D (A) and 2E (B). **P <0.01.

Additional file 4: Fig. S4. Representative images of CK8/18 and H&E staining of PCa-involved tibias. (A) Immunohistochemical staining of CK8/18 in PCa-involved tibias. (B) Representative image of H&E staining in a C4-2B-inoculated tibia with large fluorescence range.

Additional file 5: Fig. S5. Effect of Pro on the expression of TGF-β2 in MC3T3-E1 cells. Western blot of TGF-β2 in MC3T3-E1 cells co-cultured with C4-2B in 0.4 μm transwells under indicated conditions.

Additional file 6: Fig. S6. Predicted structure of Pro binding to FKBP/mTOR complex. The carboxylic end of Pro was anchored on FKBP5 by inserting into a hydrophobic cavity, mainly delineated by aromatic side chains.

Additional file 4. Unprocessed images of Western blot in Figure 7.

Additional file 4. Unprocessed images of Western blot in Figure 7.

Additional file 2: Fig. S2. Models describing the regulatory network involved in the acquisition of cold tolerance in common vetch.

Additional file 1: Fig. S1. Graphs for meta-regression analysis. CI = confidence interval. Meta-regression was performed by position (supine or semi- recumbent vs. prone or Trendelenburg), PEEP ((5mH2O≤PEEP ≤15mH2O vs. others), Place (ICU vs. OR) and Device (TPTD vs. other measurement tools other than TPTD).

Additional file 1: Fig. S1. Graphs for meta-regression analysis. CI = confidence interval. Meta-regression was performed by position (supine or semi- recumbent vs. prone or Trendelenburg), PEEP ((5mH2O≤PEEP ≤15mH2O vs. others), Place (ICU vs. OR) and Device (TPTD vs. other measurement tools other than TPTD).

Additional file 2: Fig. S2. Models describing the regulatory network involved in the acquisition of cold tolerance in common vetch.

Additional file 1: Supplementary Fig. 1. Expression and prognosis of TSPs in glioma.

Additional file 1: Supplementary Fig. 1. Expression and prognosis of TSPs in glioma.

Additional file 2: Supplementary Fig. 2. Analysis of the Spearman correlation coefficients between the expression of TSP2 protein and the age of patients.

Additional file 3: Supplementary Fig. 3. The source of the TSP2 protein in surgical specimens.

Additional file 4: Supplementary Fig. 4. Effects of different concentrations of TSP2 protein on the proliferation of U251 cells.

Additional file 1: Figure S1. Mechanism of MET activation. a MET is the receptor of hepatocyte growth factor (HGF). The α and β chains encompass the remainder of the extracellular domain, the juxtamembrane domain, and the kinase domain. The intracellular part of MET contains a juxtamembrane region responsible for signal regulation and receptor degradation, a catalytic region with enzyme activity, and a C-terminal region acting as a docking site for adaptor proteins. b The binding...

Additional file 1: Fig. S1. COL12A1 mRNA expression is upregulated in clinical iCCA. (A) Boxplot to show COL12A1 mRNA expression levels in clinical iCCA, CHC, FNH and adjacent nontumor tissues samples from GSE32879 dataset. ns, p ≥ 0.05, **** p < 0.00001, using two-tailed unpaired Student's t test. Boxplot to show COL12A1 mRNA expression levels in clinical iCCA and HCC tissue samples form TCGA, GSE76297 or our in-house dataset, respectively. * p < 0.05; ****...

Additional file 4: Figure S3. MiR-194-5p affects the expression of SOCS2 which reverses the effects of miR-194-5p on cell proliferation and migration. (A) and (B), the number of colonies or cells was counted in the control group, miR-194-5p mimic or miR-194-5p inhibitor group, miR-194-5p mimic or miR-194-5p inhibitor group and SOCS2 overexpression plasmid or si-SOCS2 co-transfected group by colony formation and Transwell assays. (C) HE staining (scale bar = 100 μm) and the expression of...