Additional file 2: Fig. S2. qRT-PCR validation of 10-mRNA expression levels in gastric cancer cell lines (AGS and MGC-803) and normal cell lines (GES-1). ** indicates P < 0.01, and * indicates P < 0.05.

Additional file 1: Fig. S1. Schematic representation of LIPUS irradiation system. The platform includes a function generator (Tektronix, USA), a power amplifier (T&C Power Conversion, Inc., USA), a 10-cm culture dish, a 6-cm high agarose phantom, and an ultrasonic transducer fixed by a tube clamp.

Additional file 5: Fig. S5. The mRNA expression of NF-κB in skin allografts measured by qRT-PCR. The mRNA expression of NF-κB in skin allografts measured by qRT-PCR showed no significant difference among different groups. Error bars represent mean ± SD. Statistical significance assessed by unpaired two-tailed t-test. (n = 4 per group).

Additional file 6: Fig. S6. CD169 + immunohistochemical staining of skin allografts. Control group contained massive infiltration of CD169 + cells in comparison to the C-EVs and LIPUS-EVs group. Scale bar = 50 μm. (Representative images from 3 different mice per group).

Additional file 4: Figure S1. Verification of transcriptome accuracy by qRT–PCR. The x-axis represents the DETs, and the y-axis represents the relative expression of the gene. DETs above the horizontal line are upregulated and those below the horizontal line are downregulated. qRT–PCR: quantitative real-time PCR; DETs: differentially expressed transcripts.

Additional file 10: Figure S4. The top 30 immune- or inflammation-related GO terms of the differentially expressed (DE) lncRNAs. The significantly enriched GO terms in biological process (red column), cellular component (blue column) and molecular function (green column) terms at 12 hpi (a), 24 hpi (b) and 36 dpi (c). The left Y-axis shows the number of DElncRNAs. The X-axis shows the name of the GO terms.

Additional file 3: Figure S3. Change of stroma percentage between primary site and bone metastasis in Luminal type, HER2 positive type and triple negative type breast cancers.

Additional file 6: Figure S1. The circRNA-miRNA networks comprising the 22 DECs (blue square) and their targets of 118 miRNAs (red rhombus) in moso bamboo.

Additional file 1: Fig. S1. The LNM-DEGs associated with OS for STAD patients in TCGA Datasets based on uniCox (A) and multiCox (B) proportional hazard.

Additional file 3: Fig. S3. The original images of WB in this study to detect the protein expression level of 10-mRNA signature and GAPDH which as the internal control.

Additional file 5: Figure S2. The top 30 immune- or inflammation-related GO terms of the differentially expressed (DE) mRNAs. The significantly enriched GO terms in biological process (red column), cellular component (blue column) and molecular function (green column) at (a) 12 hpi, 24 hpi (b) and 36 dpi (c). The left Y-axis shows the number of DEmRNAs. The X-axis shows the name of the GO terms.

Additional file 8: Figure S3. The co-localization networks of DElncRNAs and DEmRNAs at (a) 12 hpi, (b) 24 hpi and (c) 36 dpi, respectively. The diamonds represent the DElncRNAs and circles represent the DEmRNAs. The up- and down-regulated RNAs are indicated by red and blue colors, respectively.

Additional file 1: Supplementary Figure1. The serum AMHconcentrations in the population with PCOS and PCOM and the control group basedon different BMIs. PCOS,polycystic ovary syndrome; PCOM, polycystic ovary morphology; AMH, anti-Müllerian hormone; BMI, body mass index.

Additional file 2: Supplementary Figure 2. ROC curves for thediagnosis of PCOM based on different BMIs. BMI, body mass index; AUC,area under the curve.

Additional file 3: Supplementary Figure3. The serum AMHconcentrations in the PCOS and the PCOM groups based on the length of menstrualcycle. PCOS, polycystic ovary syndrome; PCOM, polycystic ovary morphology; AMH,anti-Müllerian hormone. PCOS (<180): patients with PCOS with menstrual cyclelength <180 days; PCOS (≥180): patients with PCOS with menstrual cyclelength ≥180 days.

Additional file 1: Supplementary Fig. 1. YTHDF2 knockdown by siYTHDF2-2 promoted GC cell proliferation and migration. A Western blot result showing the knockdown efficiency of YTHDF2 in HGC27 cells. B YTHDF2 knockdown promoted cell viability of HGC27 cells via CCK8 assay. C YTHDF2 knockdown promoted HGC27 cell migration via transwell assay.

Additional file 2: Supplementary Fig. 2. The functional link between YTHDF2 and PPP2CA in GC cells. A Cell migration capacity was determined by transwell assay in YTHDF2 overexpression cells transfected with siPPP2CA-2 and siNC. Western blot confirmed the knockdown efficiency of PPP2CA. B PPP2CA overexpression suppressed the phenotype induced by YTHDF2 knockdown in AGS cells via transwell assay. FLAG-tagged PPP2CA was detected by western blotting.

Additional file 7: Figure S7. Representative FACS plots and bar graphs showing CD45R/B220+ B cells in the spleens of B-NDG mice in non-IR + PBS, IR + PBS, IR + GA, IR-OEa8 + PBS, and IR-OEa8 (PM69) + GA groups.

Additional file 2: Figure S1. (A) The results of wound healing assay showed impaired cell motility after knockdown of EGR1. (B) The results of wound healing assay showed enhanced cell motility after overexpression of EGR1. (C) The predicted RNA correlation of EGR1 and TWIST1 in pancreatic cancer. (D) The ChIP-qPCR results of EGR1 and SNAI1 promoter. (E) The effect of SNAI2 overexpression and kncokdown were verified by qRT-PCR and western blot assays. *: p <...

Additional file 1. A: integration of digital dental casts into 3D facial image (cheek retractor). B: integration of 3D facial image (smile) with 3D facial image (cheek retractor). Red regions indicate the registration reference areas

Additional file 2: Figure S2. The standard IHC PD L1 scores in Adeno-NEPC.

Additional file 3: Figure S3. Co expression of CgA, SYN, CD56 and NSE in Adeno-NEPC.

Additional file 3: Fig. S3. The phosphorylation level of STAT3 is proportional to the expression of KPNA2, and this change is not dependent on the degree of cell confluence. (A) HUVEC were infected with different amounts of Ad-KPNA2 to examine the effect of overexpression of KPNA2 to different degrees, and the protein expression levels of KPNA2, P-STAT3, and STAT3 were detected by Western Blot. Relative quantitative statistical analysis was performed on protein expression levels. (B)...

Additional file 3: Figure S3. IPA network pathway analysis. The IPA network pathway analysis revealed that these differentially expressed lipids were related to NF-κB signaling, PI3K/AKT signaling, and JAK/STAT signaling. IPA, ingenuity pathway analysis; CP, classical signaling pathway.

Additional file 2: Fig. S2. Combination of FPR1 and FPR2 inhibitors improves inflammation, but efficacy improvement is not significant. (A, B) Kidney tissues were sectioned for histological examination. Scale bar, 100 μm. Representative images from one experiment out of three are shown. (C) Serum from sham surgery, IRI, WRW4-treated, and CsH/WRW4-treated rats was sampled at 24 h after surgery. Serum creatinine and blood urea nitrogen levels were measured. (D) MDA levels in rat kidneys were...