2 Works

HIV-1 fusion is disrupted by addition of deoxygluose and is rescued with addition of cholesterol

Sergi Padilla-Parra
There has been resurgence in determining the role of host metabolism in viral infection yet deciphering how the metabolic state of single cells affects viral entry and fusion remains unknown. Here, we have developed a novel assay multiplexing genetically-encoded biosensors with single virus tracking (SVT) to evaluate the influence of global metabolic processes on the success rate of virus entry in single cells. We found that cells with a lower ATP:ADP ratio prior to virus...

Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading

Lisa Rodermund
Xist RNA directs the process of X-chromosome inactivation in mammals by spreading in cis along the chromosome from which it is transcribed and recruiting chromatin modifiers to silence gene transcription. To elucidate mechanisms of Xist RNA cis-confinement, we established a sequential dual-color labeling, super-resolution imaging approach to trace individual Xist RNA molecules over time, enabling us to define fundamental parameters of spreading. We demonstrate a feedback mechanism linking Xist RNA synthesis and degradation, and an...

Registration Year

  • 2021
    2

Resource Types

  • Image
    2

Affiliations

  • University of Oxford
    2
  • Wellcome Centre for Human Genetics
    1
  • Frederick National Laboratory for Cancer Research
    1
  • Antoni van Leeuwenhoek Hospital
    1