Quantification of Bone Marrow Compartments in Histological Sections

Josefine Tratwal & Olaia Naveiras
We report the development of a semi-automated image analysis plug-in, MarrowQuant, which employs the open-source software QuPath, to systematically quantify multiple bone components in H&E sections in an unbiased manner. MarrowQuant discerns and quantifies the areas occupied by bone, adipocyte ghosts, hematopoietic cells and the interstitial/microvascular compartment. A separate feature, AdipoQuant, fragments adipocyte ghosts in H&E-stained sections of extramedullary adipose tissue sections to render adipocyte area and size distribution. Quantification of BM hematopoietic cellularity with...

Registration Year

  • 2021
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Resource Types

  • Image
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Affiliations

  • Hospital Universitario 12 De Octubre
    1
  • École Polytechnique Fédérale de Lausanne
    1
  • Washington University Medical Center
    1
  • Harvard University
    1
  • University Hospital of Lausanne
    1