Hepatitis E virus (HEV) infection is primarily manifesting as acute hepatitis, but extra-hepatic replication and injury are frequently reported. During the study period, we discovered two acute myeloid leukaemia (AML) patients infected with HEV genotype 3 and 4, respectively, and HEV RNA and/or viral proteins were persistently detected in the bone marrow of both patients. The finding suggests that HEV can replicate in human bone marrow as it may serve as a new target site...

To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Supplemental material, sj-tiff-3-tah-10.1177_20406207221146031 for Antihuman T lymphocyte porcine immunoglobulin combined with cyclosporine as first-line immunosuppressive therapy for severe aplastic anemia in China: a large single-center, 10-year retrospective study by Wenrui Yang, Xu Liu, Xin Zhao, Li Zhang, Guangxin Peng, Lei Ye, Kang Zhou, Yuan Li, Jianping Li, Huihui Fan, Yang Yang, Youzhen Xiong, Liping Jing and Fengkui Zhang in Therapeutic Advances in Hematology

Additional file 4: Figure S1. Phylogenetic analysis of Pvs230 sequences from global P. vivax populations. The maximum-likelihood phylogenetic tree reconstructed based on alignment by ClustalW with bootstrap analysis to assess clade support (500 replicates) was shown for Pvs230 global isolates. The global isolates were clustered into three main groups, the branches of which are shown in red (group 1), green (group 2), and blue (group 3).

Supplementary Material 7

Additional file 1: Figure S2. Sorting of CD4+ T cells in CD4+CD25+Foxp3+Treg cells and CD3+CD8− cells in CD3+CD8−IL-17+Th17 cells analyzed by flow cytometry. Fig. S2 is related to Fig. 5A.

Additional file 1: Figure S3. Propofol inhibited invasion and migration of lung cancer cells. A Effect of different doses (0.3 μg/mL, 3 μg/mL, 30 μg/mL) of propofol on lung cancer cell viability was measured by CCK-8 assay; B Cell invasion were detected using Transwell assay; C Cell migration was detected Transwell assay; D Positive rate of E-cadherin protein was detected by immunocytochemistry and the stronger red fluorescence meant the higher positive rate. All experiments were...

Additional file 4: Fig. S3. A Schematic diagram of the binding sites of miR-532-3p on CEBPA-DT. B Relative luciferase activities of miR-532-3p binding sites on CEBPA-DT were measured by luciferase reporter assays in indicated 293T cells. C RIP-qPCR assays showed the enrichment of CEBPA-DT on AGO2 relative to IgG in indicated HCCLM9 cells. D Subcellular localization of hnRNPC in indicated HCCLM9 and SNU-449 cells were measured by subcellular protein fractionations and western blot assays. E-F...

Additional file 4: Fig. S3. A Schematic diagram of the binding sites of miR-532-3p on CEBPA-DT. B Relative luciferase activities of miR-532-3p binding sites on CEBPA-DT were measured by luciferase reporter assays in indicated 293T cells. C RIP-qPCR assays showed the enrichment of CEBPA-DT on AGO2 relative to IgG in indicated HCCLM9 cells. D Subcellular localization of hnRNPC in indicated HCCLM9 and SNU-449 cells were measured by subcellular protein fractionations and western blot assays. E-F...

Supplemental material, sj-eps-2-tam-10.1177_17588359221133546 for Circulating tumour DNA biomarkers in savolitinib-treated patients with non-small cell lung cancer harbouring MET exon 14 skipping alterations: a post hoc analysis of a pivotal phase 2 study by Yongfeng Yu, Yongxin Ren, Jian Fang, Lejie Cao, Zongan Liang, Qisen Guo, Sen Han, Zimei Ji, Ye Wang, Yulan Sun, Yuan Chen, Xingya Li, Hua Xu, Jianying Zhou, Liyan Jiang, Ying Cheng, Zhigang Han, Jianhua Shi, Gongyan Chen, Rui Ma, Yun Fan,...

Additional file 1: Figure sup 1. Opposing effects of apoE2 and apoE4 on Iba1 proteinlevels and their correlation with the amount of myelin debris in the corpuscallosum of apoE-TR mice upon cuprizone-induced demyelination. ApoE-TR micewere fed with either normal diet (CTL, n=5/genotype), or CPZ-containing diet(CPZ, n=5-6/genotype) for four weeks. (A,B) Iba1 protein level inthe CC region was assessed by Western blot (WB) analysis, and the fold change (CPZ vs. CTL) wasquantified. (C) A negative correlation...

Objectives: Caffeine has been shown to reduce the incidence of bronchopulmonary dysplasia (BPD). To investigate the protective mechanism of caffeine in a hyperoxia-based cell model of BPD in vitro. Methods: Type II alveolar epithelial cells (AECs II) were isolated and randomly divided into 6 groups: the normal, hyperoxia, caffeine (50 μM caffeine), antagonist (5 μM ZM241385), agonist (5 μM CGS21680), and DMSO groups. Transfection with siRNA against adenosine A2A receptor (siA2AR) was performed in AECs...

Objectives: Caffeine has been shown to reduce the incidence of bronchopulmonary dysplasia (BPD). To investigate the protective mechanism of caffeine in a hyperoxia-based cell model of BPD in vitro. Methods: Type II alveolar epithelial cells (AECs II) were isolated and randomly divided into 6 groups: the normal, hyperoxia, caffeine (50 μM caffeine), antagonist (5 μM ZM241385), agonist (5 μM CGS21680), and DMSO groups. Transfection with siRNA against adenosine A2A receptor (siA2AR) was performed in AECs...

Additional file 1: Figure sup 1. Opposing effects of apoE2 and apoE4 on Iba1 proteinlevels and their correlation with the amount of myelin debris in the corpuscallosum of apoE-TR mice upon cuprizone-induced demyelination. ApoE-TR micewere fed with either normal diet (CTL, n=5/genotype), or CPZ-containing diet(CPZ, n=5-6/genotype) for four weeks. (A,B) Iba1 protein level inthe CC region was assessed by Western blot (WB) analysis, and the fold change (CPZ vs. CTL) wasquantified. (C) A negative correlation...

Additional file 1: Fig. S1. Further validation of the effects of hypoxia on mESC differentiation. (A) Hypoxia significantly repressed the mRNA expression of endoderm markers (Sox17 and Cxcr4) in differentiating AB2.2 mESCs. (B) Hypoxia significantly repressed the mRNA expression of an endothelial marker (Pecam1) and a smooth muscle marker (Acta2) in differentiating AB2.2 mESCs. (C) Hypoxia significantly repressed the expression of mesendoderm markers (T, Eomes, Mesp1, and Gsc) in differentiating R1 mESCs. (D) Hypoxia significantly...

Additional file 2: Fig. S2. The effects of hypoxia on AB2.2 mESC differentiation performed with a high-serum differentiation medium. The differentiation in the high-serum differentiation medium was non-lineage-prone. In the differentiation performed with the high-serum medium, the expressions of (A) mesendoderm markers (T, Eomes, Mesp1, and Gsc) and (B) ectoderm markers (Pax6 and Nestin) were downregulated and upregulated by hypoxia, respectively. *, significant (P<0.05).

Additional file 4: Fig. S4. The analyses performed on the RNA-seq data of AB2.2 mESCs on differentiation day 2 under normoxia and hypoxia. (A) The related levels of HIF-1 signaling-related genes in AB2.2 mESCs on differentiation day 2 under hypoxia versus normoxia. (B) GSEA showed that the HIF-1 signaling pathway activation was significantly enhanced by hypoxia. (C) The related levels of Wnt/β-Catenin pathway-related genes in AB2.2 mESCs on differentiation day 2 under hypoxia versus normoxia....

Additional file 6: Fig. S6. The Wnt/β-Catenin pathway and AB2.2 mESC differentiation were affected by IWP2. (A) Schematic diagram of mESC differentiation treated with or without IWP2 under normoxia. (B) The expression of Wnt/β-Catenin downstream targets (Sp5 and Cdx1) was severely inhibited by IWP2 in AB2.2 mESCs undergoing differentiation. (C) IWP2 repressed T protein expression (red) on differentiation day 4. Nuclei were stained with DPAI (blue). (D) The ratios of T+ and Sox1+ cells were...

Additional file 7: Fig. S7. The supplementary data of the effects of Hif-1α on the mesendoderm differentiation and Wnt/β-Catenin pathway. (A) The expression patterns of Egln1 in the differentiating AB2.2 mESCs under normoxia and hypoxia. (B) Doxycycline (Dox)-inducible overexpression of oxygen-resistant Hif-1α was verified by western blotting analysis. A Myc tag was added to the C-terminus of Hif-1α. (C) The mRNA expression of mesendoderm markers (T, Eomes, Mesp1, and Gsc) in AB2.2 mESCs with or...

Additional file 3: Fig. S3. The supplementary analyses performed on the RNA-seq data of AB2.2 mESCs on differentiation day 4 under normoxia and hypoxia. (A) PCA of RNA-seq data from normoxia and hypoxia groups. The PCA included three biological repeats for each group. (B) The volcano plot showed the differentially expressed genes (DEGs). |Log2(Fold Change)|>1 and adjusted P-value<0.05 were used as the cutoff criteria. (C) The related levels of Hh signaling-related genes in AB2.2 mESCs...

Additional file 3: Fig. S3. The supplementary analyses performed on the RNA-seq data of AB2.2 mESCs on differentiation day 4 under normoxia and hypoxia. (A) PCA of RNA-seq data from normoxia and hypoxia groups. The PCA included three biological repeats for each group. (B) The volcano plot showed the differentially expressed genes (DEGs). |Log2(Fold Change)|>1 and adjusted P-value<0.05 were used as the cutoff criteria. (C) The related levels of Hh signaling-related genes in AB2.2 mESCs...

Additional file 6: Figure sup 6. The key molecules that regulate microglialfunctions are up-regulated in an apoE isoform-dependent manner uponcuprizone-induced demyelination. RNA was extracted from the CC area of CTL(n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice. (A-H) The expression of genes relatedto microglial activation, inflammation, and lipid sensing (Trem2, Tyrobp,Aim2, Btk, B2m, Ctsd, C3, and Cr3) was analyzed by real-time PCR. (I-L) The expression of genes involvedin microglial migration (Mmp2 and Mmp12) and phagocytosis (Axl)were examined...

Additional file 1. The workflow and study design of the analysis steps.

Additional file 4.The expression level of immune checkpoints in high-risk and low-risk groups.

Additional file 5. Infiltration of gene expression, overall survival, mutation profile and the comparison of tumor infiltration levels in m6A-related genes with different somatic copy number alterations for LUAD and LUSC in TIMER2.0.