Additional file 1: Supplementary Figure 1. OC cell lines exhibit heterogenous biosensor responses to OCM treatment. Fluorimetric measurements of iNap (A), Perceval (B), and Peredox (C) signal were normalized to the average across all cell lines (n = 3 technical replicates per cell line). Signal was measured following 48 hours of growth in OCM or regular media and normalized to growth in control regular media for iNap (D), Perceval (E), and Peredox (F).

Additional file 2: Supplementary Figure 2. Biosensor responses to carboplatin treatment across heterogenous OC cell lines are negatively correlated with carboplatin resistance. Biosensor-expressing cell lines were treated with 100 μM carboplatin and iNap (A), Perceval (B), and Peredox (C) signal was measured at 24 and 48 hours. (D-F) Signal for each biosensor in (A-C) in treated cells was compared to that in control, untreated cells, normalized to 1 (n = 3 per condition). Normalized iNap...

Additional file 4: Figure S4. TPM1 knockdown exacerbates inflammation in the TREM2−/− mouse retina. A, B Western blot analysis (A) and quantification of TPM1 (B) in the retina of C57BL/6J (WT) and TREM2−/− mice. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test (WT vs. TREM2−/−, **p < 0.01). C, D Western blot analysis (C) and quantification of TREM2 (D) in WT mice following LPS and siCTR or siTPM1-1...

Additional file 5: Figure S5. TPM1 knockdown changes transcriptome associated with glial cells in WT mouse retina. A A heatmap showing DEGs associated with microglia and astrocytes in WT mice following treatments with PBS, or with LPS and siTPM1-1 or siCTR. B–G The expression levels of DEGs associated with M1 (B) or M2 microglia (C, D) and with A1 (E) or A2 astrocytes (F, G) in WT mice after treatment with PBS, or with LPS...

Additional file 4: Figure S1. Phylogenetic analysis of Pvs230 sequences from global P. vivax populations. The maximum-likelihood phylogenetic tree reconstructed based on alignment by ClustalW with bootstrap analysis to assess clade support (500 replicates) was shown for Pvs230 global isolates. The global isolates were clustered into three main groups, the branches of which are shown in red (group 1), green (group 2), and blue (group 3).

Additional file 10: Fig. S4. Single cell transcriptomic analysis of TLE + normal TL integrated dataset (a) UMAP representation of TLE samples integrated with TL normal data [39]. The clusters are colored and labeled according to their respective annotated cell type identity. (b) Feature plots showing log-normalized expression of canonical markers used for annotation of cell types in the TLE + normal TL integrated analysis from (a). (c) Violin plot (left) and scaled gradient feature...

Additional file 2: Supplementary figure 2. The relationship between immune-related function and prognosis of CLL patients.

Additional file 1: Figure S1. The increase rate of frequency by slope from low to high. The increase rate of frequency was defined as the frequency at a specific slope was divided by the accumulated frequency at all observed slopes that were less than the specific slope.

Supplemental material, sj-tif-4-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Additional file 1: Fig. S1 The distributions of variant allele frequencies (VAFs) of ARID1A, ARID1B, ARID2, PBRM1, SMARCA4, and SMARCB1. The median VAFs of the above genes were 16.1%, 13.4%, 13.3%, 17.2%, 15.2%, and 16.7%, respectively. Red solid line, median; black dotted line, quartiles.

Supplementary Figure 2. CircPTPRF overexpression reversed proliferation and invasion suppression of GBM in vitro caused by circPTPRF knockdown. a, b: MTS assays showed the cell viability of circPTPRF knockdown LN229 and GSC17 after circPTPRF overexpression. c, d: CircPTPRF overexpression can greatly reverse the proliferative capacity of circPTPRF knockdown LN229 and GSC17 measured by Edu assays. Scale bar = 50?m. e, f: Representative images of transwell assay to demonstrate invasion capacity can be reversed after circPTPRF...

To verify the applicability and accuracy of the permeability model in natural and laboratory samples.

Additional file 6: Fig. S6. Characteristics of Akkermansia and Mucispirillum, and their relationships with other microbiota. A Spearman correlation of adjusted relative abundance for bacteria between the CL and TH groups, adjusted relative abundance = ln(abundance+Δ), Δ = 10− 7. B Cumulative abundance distribution of cecal bacteria. C Co-occurrence networks associated with Akkermansia and Mucispirillum (ρ > 0.4, P < 0.05). D Principal coordinates analysis (PCoA) using a distance matrix of enterotypes (Bacteroides and Desulfovibrio)....

Additional file 2 Supplementary Fig. 2. A phylogenetic tree demonstrates the genetic distance among all mouse KRAB-Zfp genes. The detailed description is the same as that in Fig. 1B.

To verify the applicability and accuracy of the permeability model in natural and laboratory samples.

Additional File 5.

Previously, we reported that the mediation of Newcastle disease virus (NDV) pathogenicity by the 524YLMY527 motif depends mainly on the regulation of F protein transport to the cell surface. The virus and host determinants that govern this intracellular trafficking remain unknown. Here, we confirmed that host adaptor protein (AP) complexes are involved in NDV infection using small interfering RNA. The transport of viral F protein to the cell surface depends on host transport proteins. We...

Additional file 4: Fig. S3. A Schematic diagram of the binding sites of miR-532-3p on CEBPA-DT. B Relative luciferase activities of miR-532-3p binding sites on CEBPA-DT were measured by luciferase reporter assays in indicated 293T cells. C RIP-qPCR assays showed the enrichment of CEBPA-DT on AGO2 relative to IgG in indicated HCCLM9 cells. D Subcellular localization of hnRNPC in indicated HCCLM9 and SNU-449 cells were measured by subcellular protein fractionations and western blot assays. E-F...

Additional file 4: Fig. S3. A Schematic diagram of the binding sites of miR-532-3p on CEBPA-DT. B Relative luciferase activities of miR-532-3p binding sites on CEBPA-DT were measured by luciferase reporter assays in indicated 293T cells. C RIP-qPCR assays showed the enrichment of CEBPA-DT on AGO2 relative to IgG in indicated HCCLM9 cells. D Subcellular localization of hnRNPC in indicated HCCLM9 and SNU-449 cells were measured by subcellular protein fractionations and western blot assays. E-F...

Additional file 2: Fig. S2. Methylation status of differentially methylated sites. (A-B) Volcano plots showing DMCs in the context of CHG (A) and CHH (B). (C) Bar plots showing distribution of differences in methylation levels of DMCs.

Supplementary Material 1

Supplementary Material 2

Supplementary Material 3

Additional file 2: Figure S2. KEGG enrichment of differential expressed genes in septic patients compared with healthy person. a The volcano plot was constructed using the fold change values and P-adjust. Red dots indicate upregulated genes; blue dots indicate downregulated genes. b The heatmap of the differential gene expression, where different colors represent trends of gene expression in different tissues. c, d The enriched KEGG signaling pathways were selected to demonstrate the primary biological actions...

Additional file 3: Figure S3. Effects of cilostazol treatment on serum inflammatory factors. The level of serum TNF-α (a) and IL-1β (b) in each group (n = 6). The data are presented as the mean ± SEM, ***P < 0.001, ****P < 0.0001, and ns indicates no significant difference.