Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

Additional file 1: Figure S1. Toxicity assessment of PMVs@PLGA complexes on cardiac muscle tissue. A TUNEL apoptosis assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. B BAX IF assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. C BCL-2 IF in cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats (scale bars indicate 100 μm).

Additional file 1: Figure S1. Toxicity assessment of PMVs@PLGA complexes on cardiac muscle tissue. A TUNEL apoptosis assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. B BAX IF assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. C BCL-2 IF in cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats (scale bars indicate 100 μm).

Additional file 2: Figure S2. Schematic representation of the sequences of the dual luciferase reporter gene vectors for the 4 miRNAs.

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Additional file 1: Figure S1. The downregulation of EMCN expression is related to metastasis and recurrence in breast cancer patients. (A) Heatmap of differentially expressed genes in breast cancer from TCGA datasets. (B) Volcano plot of differentially expressed mRNAs. Red dots represent upregulated mRNAs, and blue dots represent downregulated mRNAs. (C) EMCN expression in adjacent tissues and tumor tissues was analyzed (Wilcox tests, ****p < 0.0001, **p < 0.01, **p < 0.05). (D) EMCN gene...

Additional file 12. Figure S12. Histogram representing the proportion of clusters in each group.

Additional file 13. Figure S13. Bubble plots showing the results of DEG functional enrichment analysis using GO gene sets in T cells, NK cells, and cDCs.

Additional file 14. Figure S14. Reclustering analysis of tumor-infiltrating NK cells. (a) T-distributed stochastic neighbor embedding (t-SNE) plot for NK cells. (b) t-SNE plot for the treatment group-specific distribution of NK cell subclusters. (c) Violin plots showing NK cell subcluster-specific gene profiles. (d) Histogram representing the proportion of NK cells subcluster in each group. (e-f) Violin plots showing the levels of Cd69 and Tnf of NK cell subclusters. (g) Heatmap depicting the results of GSEA...

Additional file 4. Figure S4: The effects of MSA-2 and TGF-β on chemokine production in BMDM. (a-f) Unactivated BMDMs were cultured with MSA-2 for one day, and supernatants were collected for chemokine detection.

Additional file 5: Figure S4. The correlation between LARRPM expression, 5hmC level at CSF1 promoter, CpG82 methylation level and CSF1 expression in LUAD tissues. A Correlation between CSF1 and LARRPM expression analysed using the TCGA LUAD data. r = − 0.3069, P < 0.0001 by Spearman correlation analysis. B Correlation between CSF1 and LARRPM expression analysed in our LUAD cohort. r = − 0.5774, P < 0.0001 by Spearman correlation analysis. C 5hmC levels of...

Additional file 7: Fig. S6. Depletion of LARRPM in LUAD cells enhanced the oncogenic roles of infiltrated M2 macrophages. A–F Conditioned medium (CM) was collected from co-culture of macrophages with HCC827 cells with LARRPM depletion or control HCC827 cells, or collected from only HCC827 cells with LARRPM depletion or control HCC827 cells. A Cell proliferation of HCC827 cells treated with the CM was detected using CCK-8 assays. B Cell proliferation of HCC827 cells treated with...

Additional file 5: Figure S5. Composition of splenic immune cells and CD4+ T cell subpopulations in healthy Sigirr +/+ mice and Sigirr −/− mice. (A), Frequencies of major immune populations in spleens between Sigirr +/+ mice (n = 5) and Sigirr −/− mice (n = 5). (B), Absolute number and frequency of granulocytes among 2.5 × 105 live CD45+ leukocytes derived from Sigirr +/+ and Sigirr −/−mice. (C), Representative plots of T cells (CD3+ NK1.1−),...

Additional file 7: Figure S7. Ectopic expression of SIGIRR in purified CD4+ T cells after transfection with retrovirus packaged by phoenix helper-free retrovirus producer lines. The expression of SIGIRR (50 kDa) and GFP (27 kDa) in CD4 T-cell lysates was detected by immunoblotting with GAPDH as a loading control.

Additional file 3: Supplementary Figure S3. The survival curves of necroptosis-score. A-D Kaplan–Meier curve was used to analyze the survival rate of CESC (A), OV (B), UCEC (C), and UCS (D) patients with high or low necroptosis-score. Black curves indicate low necroptosis-score and red curves indicate high necroptosis-score.

Additional file 4: Supplementary Figure S4. Statistical analysis of the number of differential NRGs and prognostic NRGs of the four GCs. Red bars show up-regulated NRGs in cancer tissue. The green bars show NRGs that are downregulated in cancer tissue. The blue bars show the sum of the differential NRGs. Yellow bars indicate prognostic NRGs.

Supplemental material, sj-jpg-2-jet-10.1177_15266028221133700 for Management Strategy and Radiologic Outcomes of Symptomatic Spontaneous Isolated Superior Mesenteric Artery Dissection Based on Angiographic Classification: The Follow-Up Experience in a Single Center by Zihui Yuan, Guofu Hu, Shi Sheng, Yun You and Jian Wang in Journal of Endovascular Therapy

Supplemental material, sj-jpg-2-jet-10.1177_15266028221133700 for Management Strategy and Radiologic Outcomes of Symptomatic Spontaneous Isolated Superior Mesenteric Artery Dissection Based on Angiographic Classification: The Follow-Up Experience in a Single Center by Zihui Yuan, Guofu Hu, Shi Sheng, Yun You and Jian Wang in Journal of Endovascular Therapy

Additional file 1: Supplementary Figure 1. OC cell lines exhibit heterogenous biosensor responses to OCM treatment. Fluorimetric measurements of iNap (A), Perceval (B), and Peredox (C) signal were normalized to the average across all cell lines (n = 3 technical replicates per cell line). Signal was measured following 48 hours of growth in OCM or regular media and normalized to growth in control regular media for iNap (D), Perceval (E), and Peredox (F).

Additional file 2: Supplementary Figure 2. Biosensor responses to carboplatin treatment across heterogenous OC cell lines are negatively correlated with carboplatin resistance. Biosensor-expressing cell lines were treated with 100 μM carboplatin and iNap (A), Perceval (B), and Peredox (C) signal was measured at 24 and 48 hours. (D-F) Signal for each biosensor in (A-C) in treated cells was compared to that in control, untreated cells, normalized to 1 (n = 3 per condition). Normalized iNap...

Additional file 4: Figure S4. TPM1 knockdown exacerbates inflammation in the TREM2−/− mouse retina. A, B Western blot analysis (A) and quantification of TPM1 (B) in the retina of C57BL/6J (WT) and TREM2−/− mice. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test (WT vs. TREM2−/−, **p < 0.01). C, D Western blot analysis (C) and quantification of TREM2 (D) in WT mice following LPS and siCTR or siTPM1-1...