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FIGURES 21‒22. Macraspis clavata adults. 21 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES 21‒22. Macraspis clavata adults. 21, Adult on a flower of Melastomataceae (Tibouchina sp.); 22, adult reared on laboratory from larvae collected. Scale bar = 5 mm for Fig. 22.

FIGURES 14‒20. Macraspis clavata third instar. 14‒16 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES 14‒20. Macraspis clavata third instar. 14‒16) Protibiotarsus‒pretarsus, mesotibiotarsus‒pretarsus, and metatibiotar- sus‒pretarsus; outer. 17, Venter of abdominal segment X. 18‒20, Propretarsus, mesopretarsus, and metapretarsus. Scale bars = 1 mm for Figs. 14‒17 and 0.5 mm for Figs. 18‒20.

FIGURES 14‒20. Macraspis clavata third instar. 14‒16 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES 14‒20. Macraspis clavata third instar. 14‒16) Protibiotarsus‒pretarsus, mesotibiotarsus‒pretarsus, and metatibiotar- sus‒pretarsus; outer. 17, Venter of abdominal segment X. 18‒20, Propretarsus, mesopretarsus, and metapretarsus. Scale bars = 1 mm for Figs. 14‒17 and 0.5 mm for Figs. 18‒20.

FIGURES 10‒13. Macraspis clavata third instar. 10, 13 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES 10‒13. Macraspis clavata third instar. 10, 13, Left mandible (ventral, dorsal). 11‒12, right mandible (ventral, dorsal). Scale bars = 1 mm.

FIGURES 10‒13. Macraspis clavata third instar. 10, 13 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES 10‒13. Macraspis clavata third instar. 10, 13, Left mandible (ventral, dorsal). 11‒12, right mandible (ventral, dorsal). Scale bars = 1 mm.

FIGURES 6‒9. Macraspis clavata third instar. 6 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES 6‒9. Macraspis clavata third instar. 6, Right maxilla, dorsal; 7, stridulatory area of right maxilla; 8, right mala, inner; 9, epipharynx. Scale bars = 1 mm for Figs. 6, 9 and 0.5 mm for Figs. 7‒8.

FIGURES 6‒9. Macraspis clavata third instar. 6 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES 6‒9. Macraspis clavata third instar. 6, Right maxilla, dorsal; 7, stridulatory area of right maxilla; 8, right mala, inner; 9, epipharynx. Scale bars = 1 mm for Figs. 6, 9 and 0.5 mm for Figs. 7‒8.

FIGURES. 1‒5. Macraspis clavata third instar. 1 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES. 1‒5. Macraspis clavata third instar. 1, Habitus, lateral; 2, abdominal and thoracic spiracles, t, thoracic spiracle, I‒VIII, abdominal spiracle I‒VIII; 3, head, frontal. 4‒5, Antennomere III‒IV (dorsal, ventral). Scale bars = 3 mm for Fig. 1, 0.5 mm for Fig. 2, 1 cm for Fig. 3, 1 cm for Figs. 4‒5.

FIGURES. 1‒5. Macraspis clavata third instar. 1 in Description of the third instar of Macraspis clavata (Olivier, 1789) (Coleoptera: Scarabaeidae: Rutelinae)

Rone A.F. Medeiros, Carlos A. V. Frazão, Paschoal C. Grossi & Juares Fuhrmann
FIGURES. 1‒5. Macraspis clavata third instar. 1, Habitus, lateral; 2, abdominal and thoracic spiracles, t, thoracic spiracle, I‒VIII, abdominal spiracle I‒VIII; 3, head, frontal. 4‒5, Antennomere III‒IV (dorsal, ventral). Scale bars = 3 mm for Fig. 1, 0.5 mm for Fig. 2, 1 cm for Fig. 3, 1 cm for Figs. 4‒5.

MOESM5 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 5: Figure S5. Sensitivity analysis evaluating the impact of individual studies on the pooled results. (A) MMP-1 and invasion. (B) MMP-1 and differentiation. (C) MMP-2 and invasion. (D) MMP-2 and differentiation. (E) MMP-9 and invasion. (F) MMP-9 and differentiation. (G) MMP-9 and stage. (H) MMP-9 and gender. (I) VEGF and invasion. (J) VEGF and differentiation. (K) MMP-9 and VEGF coexpression.

MOESM5 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 5: Figure S5. Sensitivity analysis evaluating the impact of individual studies on the pooled results. (A) MMP-1 and invasion. (B) MMP-1 and differentiation. (C) MMP-2 and invasion. (D) MMP-2 and differentiation. (E) MMP-9 and invasion. (F) MMP-9 and differentiation. (G) MMP-9 and stage. (H) MMP-9 and gender. (I) VEGF and invasion. (J) VEGF and differentiation. (K) MMP-9 and VEGF coexpression.

MOESM3 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 3: Figure S3. Association between MMP-9 expression and retinoblastoma clinical stage, patient gender, and VEGF expression. (A) MMP-9 and clinical stage. (B) MMP-9 and patient gender. (C) MMP-9 and VEGF coexpression. The forest plots on the left side show the results of the random-effects model generated using Review Manager. The forest plots on the right side were generated using MetaXL with a quality-effects model.

MOESM6 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 6: Figure S6. Funnel plot for publication bias. (A) Tumor invasion. (B) Tumor differentiation. (C) Clinical stage. (D) Gender. (E) Coexpression of MMP-9 and VEGF.

MOESM3 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 3: Figure S3. Association between MMP-9 expression and retinoblastoma clinical stage, patient gender, and VEGF expression. (A) MMP-9 and clinical stage. (B) MMP-9 and patient gender. (C) MMP-9 and VEGF coexpression. The forest plots on the left side show the results of the random-effects model generated using Review Manager. The forest plots on the right side were generated using MetaXL with a quality-effects model.

MOESM4 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 4: Figure S4. Proteinâ protein interaction (PPI) networks between MMP-1, MMP-2, MMP-9 and VEGF. Edges with different colors represent proteinâ protein associations. Blue edges represent the association from curated databases. Yellow edges have confirmed association by text mining. Purple edges represent the protein homology.

MOESM4 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 4: Figure S4. Proteinâ protein interaction (PPI) networks between MMP-1, MMP-2, MMP-9 and VEGF. Edges with different colors represent proteinâ protein associations. Blue edges represent the association from curated databases. Yellow edges have confirmed association by text mining. Purple edges represent the protein homology.

MOESM6 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 6: Figure S6. Funnel plot for publication bias. (A) Tumor invasion. (B) Tumor differentiation. (C) Clinical stage. (D) Gender. (E) Coexpression of MMP-9 and VEGF.

MOESM2 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 2: Figure S2. Association between MMP/VEGF expression and retinoblastoma differentiation. (A) MMP-1. (B) MMP-2. (C) MMP-9. (D) VEGF. The forest plots on the left side show the results of the random-effects model generated using Review Manager. The forest plots on the right side were generated using MetaXL with a quality-effects model.

MOESM2 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 2: Figure S2. Association between MMP/VEGF expression and retinoblastoma differentiation. (A) MMP-1. (B) MMP-2. (C) MMP-9. (D) VEGF. The forest plots on the left side show the results of the random-effects model generated using Review Manager. The forest plots on the right side were generated using MetaXL with a quality-effects model.

Additional file 4: of G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

F. Berardinelli, M. Tanori, D. Muoio, M. Buccarelli, A. Di Masi, S. Leone, L. Ricci-Vitiani, R. Pallini, M. Mancuso & A. Antoccia
Figure S4. Silencing of CHK1 increases GSC response to low concentrations of RHPS4. Protein levels of GSC #163 stably expressing either a non-targeting control shRNA (NTC) or three different shRNAs targeting human CHK1 (named shCHK1 B5, E1 and F11) are shown (A). Densitometric analysis confirmed the significant reduction of CHK1 protein levels in shCHK1 cell lines (B) and a similar reduction was also observed by means of qRT-PCR (C). Growth curves showing the effect of...

Additional file 3: of G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

F. Berardinelli, M. Tanori, D. Muoio, M. Buccarelli, A. Di Masi, S. Leone, L. Ricci-Vitiani, R. Pallini, M. Mancuso & A. Antoccia
Figure S3. Telomere length analysis in cancer stem-like cells treated with RHPS4. Telomere lengths were analyzed using centromere-calibrated QFISH. Representative images of metaphase spreads from U251MG-Adh and -Sph cells (A). Box Plot of telomere lengths in untreated and RHPS4 treated U251MG-Adh, U251MG-Sph, GSCs#1, #61, #83 and #163. Box plot represents means and quartiles, whiskers represent s.d. and points represent outliers. (B) Percentage of telomeres shorter than 5 T/C% (red bars), comprised between 6 and 10...

MOESM1 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 1: Figure S1. Association between MMP/VEGF expression and retinoblastoma invasion. (A) MMP-1. (B) MMP-2. (C) MMP-9. (D) VEGF. The forest plots on the left side show the results of the random-effects model generated using Review Manager. The forest plots on the right side were generated using MetaXL with a quality-effects model.

Additional file 4: of G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

F. Berardinelli, M. Tanori, D. Muoio, M. Buccarelli, A. Di Masi, S. Leone, L. Ricci-Vitiani, R. Pallini, M. Mancuso & A. Antoccia
Figure S4. Silencing of CHK1 increases GSC response to low concentrations of RHPS4. Protein levels of GSC #163 stably expressing either a non-targeting control shRNA (NTC) or three different shRNAs targeting human CHK1 (named shCHK1 B5, E1 and F11) are shown (A). Densitometric analysis confirmed the significant reduction of CHK1 protein levels in shCHK1 cell lines (B) and a similar reduction was also observed by means of qRT-PCR (C). Growth curves showing the effect of...

Additional file 3: of G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

F. Berardinelli, M. Tanori, D. Muoio, M. Buccarelli, A. Di Masi, S. Leone, L. Ricci-Vitiani, R. Pallini, M. Mancuso & A. Antoccia
Figure S3. Telomere length analysis in cancer stem-like cells treated with RHPS4. Telomere lengths were analyzed using centromere-calibrated QFISH. Representative images of metaphase spreads from U251MG-Adh and -Sph cells (A). Box Plot of telomere lengths in untreated and RHPS4 treated U251MG-Adh, U251MG-Sph, GSCs#1, #61, #83 and #163. Box plot represents means and quartiles, whiskers represent s.d. and points represent outliers. (B) Percentage of telomeres shorter than 5 T/C% (red bars), comprised between 6 and 10...

MOESM1 of Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Jingyi Zhu, Xi Zhang, Liqianyu Ai, Rongdi Yuan & Jian Ye
Additional file 1: Figure S1. Association between MMP/VEGF expression and retinoblastoma invasion. (A) MMP-1. (B) MMP-2. (C) MMP-9. (D) VEGF. The forest plots on the left side show the results of the random-effects model generated using Review Manager. The forest plots on the right side were generated using MetaXL with a quality-effects model.

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