27,172 Works

Qualitative cellulolytic activity of the recombinant enzyme TlicBgluc1 on carboxymethylcellulose (CMC) and cellobiose substrates.

Ana Elizabeth Araújo
(A) TlicBgluc1 exhibits affinity for CMC and cellobiose under ideal reaction conditions. EV-transfected colonies exhibited no hydrolytic activity, while positive colonies were able to hydrolyze substrates, observed through a degradation halo. (B) Effect of temperature on TlicBgluc1 activity. The enzyme may act on substrate degradation between 28 ° C and 45 ° C, and higher activity at 45 ° C. Higher temperatures may be considered to limit this specific enzyme activity. (C) effect of pH...

Qualitative cellulolytic activity of the recombinant enzyme TlicBgluc1 on carboxymethylcellulose (CMC) and cellobiose substrates.

Ana Elizabeth Araújo
(A) TlicBgluc1 exhibits affinity for CMC and cellobiose under ideal reaction conditions. EV-transfected colonies exhibited no hydrolytic activity, while positive colonies were able to hydrolyze substrates, observed through a degradation halo. (B) Effect of temperature on TlicBgluc1 activity. The enzyme may act on substrate degradation between 28 ° C and 45 ° C, and higher activity at 45 ° C. Higher temperatures may be considered to limit this specific enzyme activity. (C) effect of pH...

Supplementary 2: Recombinant P. pastoris colonies-confirmation.

Ana Elizabeth Araújo
To confirm the recombinant yeast-colonies, were used a combination of specific primers with vector-gene annealing and gene-gene annealing(A) Colonies 1 to 9: forward primer annealing on p-GAPZα-B vector and reverse primer annealing on TlicBgluc1 gene ORF; Colonies 1.1 to 9.9: forward and reverse primers annealing on TlicBgluc1 gene ORF. Colony 3 and 3.1 are not recombinant. (B) Expression-cassette confirmation by restriction analysis. Digests: PstI and EcoRI (900 bp); PstI and SalI (1473 bp) and EcoRI...

Supplementary 2: Recombinant P. pastoris colonies-confirmation.

Ana Elizabeth Araújo
To confirm the recombinant yeast-colonies, were used a combination of specific primers with vector-gene annealing and gene-gene annealing(A) Colonies 1 to 9: forward primer annealing on p-GAPZα-B vector and reverse primer annealing on TlicBgluc1 gene ORF; Colonies 1.1 to 9.9: forward and reverse primers annealing on TlicBgluc1 gene ORF. Colony 3 and 3.1 are not recombinant. (B) Expression-cassette confirmation by restriction analysis. Digests: PstI and EcoRI (900 bp); PstI and SalI (1473 bp) and EcoRI...

Supplementary 3: Degradation of sugarcane biomass by caterpillars.

Ana Elizabeth Araújo
A) Diatraea saccharalis B) Telchin licus licus. C) Sugarcane stalks consumed by the caterpillar Telchin licus licus (gallery). Source: Mendonça et al, 1996.

Supplementary 3: Degradation of sugarcane biomass by caterpillars.

Ana Elizabeth Araújo
A) Diatraea saccharalis B) Telchin licus licus. C) Sugarcane stalks consumed by the caterpillar Telchin licus licus (gallery). Source: Mendonça et al, 1996.

Alignment of amino acid sequence of β-glucosidases of the glycoside hydrolase-1 family in Lepidoptera.

Ana Elizabeth Araújo
Putative T. licus licus β-glucosidase (described in this paper) was compared with two other S. frugiperda (AAC06038.1) and P. xylostella (XP_011557578.1) glucosidases. The dashed line underlines the predicted relatively conserved signal peptide cleavage site between all enzymes. The underlined sequences demonstrate the highly conserved NEPW (184 - 187) and TENG (384 - 391) catalytic motifs. Glutamic acid residues (E marked with an arrowhead) are responsible for glycosyl hydrolase activity. The letters in bold correspond to...

Alignment of amino acid sequence of β-glucosidases of the glycoside hydrolase-1 family in Lepidoptera.

Ana Elizabeth Araújo
Putative T. licus licus β-glucosidase (described in this paper) was compared with two other S. frugiperda (AAC06038.1) and P. xylostella (XP_011557578.1) glucosidases. The dashed line underlines the predicted relatively conserved signal peptide cleavage site between all enzymes. The underlined sequences demonstrate the highly conserved NEPW (184 - 187) and TENG (384 - 391) catalytic motifs. Glutamic acid residues (E marked with an arrowhead) are responsible for glycosyl hydrolase activity. The letters in bold correspond to...

TlicBgluc1gene expression pattern at different stages of insect life.

Ana Elizabeth Araújo
(A) Expression of the new TlicBgluc gene was evaluated by qPCR at different stages of insect life (egg, larval stage early –ELI, late larval stage - LLI, pupa, moth) to determine the stage at which the gene exhibits higher expression. According to the results, the gene was widely expressed throughout the insect's life cycle, but expression increased significantly at the late larval stage (LLI), when the insect required most glucose utilization. (B) In the larval...

TlicBgluc1gene expression pattern at different stages of insect life.

Ana Elizabeth Araújo
(A) Expression of the new TlicBgluc gene was evaluated by qPCR at different stages of insect life (egg, larval stage early –ELI, late larval stage - LLI, pupa, moth) to determine the stage at which the gene exhibits higher expression. According to the results, the gene was widely expressed throughout the insect's life cycle, but expression increased significantly at the late larval stage (LLI), when the insect required most glucose utilization. (B) In the larval...

Analysis of recombinant protein expression by SDS-PAGE and Western Blotting.

Ana Elizabeth Araújo
(A) SDS-PAGE analysis of empty vector colonies and TlicBgluc1-pGAPZα-B transfected P. pastoris colonies. The empty vector transfected (EV) fragment exhibits no corresponding 50 kDa band according to the predicted mw. Colonies 1 and 2 exhibit low protein expression and colonies 3 to 5 exhibit high enzyme expression predicted by MW. (B) Western blotting analysis confirms the presence of the enzyme TlicBgluc1 in colony expression supernatant 3 to 5 with a single band immuno-labeled with the...

Analysis of recombinant protein expression by SDS-PAGE and Western Blotting.

Ana Elizabeth Araújo
(A) SDS-PAGE analysis of empty vector colonies and TlicBgluc1-pGAPZα-B transfected P. pastoris colonies. The empty vector transfected (EV) fragment exhibits no corresponding 50 kDa band according to the predicted mw. Colonies 1 and 2 exhibit low protein expression and colonies 3 to 5 exhibit high enzyme expression predicted by MW. (B) Western blotting analysis confirms the presence of the enzyme TlicBgluc1 in colony expression supernatant 3 to 5 with a single band immuno-labeled with the...

Enzymatic activity of TlicBgluc1 by the DNS-mediated reducing sugar quantitation assay.

Ana Elizabeth Araújo
The enzyme-enriched supernatant of P. pastoris expression was incubated with CMC solution and the release of reducing sugar by the hydrolytic process was quantified in the DNS assay. The supernatant was incubated with the cellulosic substrate for up to 20 minutes. Uninoculated YPD medium and EV-transfected inoculated medium were also detected to discard residual reducing sugars present in the reaction medium and in the basal activity of other P. pastoris hydrolases. TlicBgluc1 activity can enrich...

Enzymatic activity of TlicBgluc1 by the DNS-mediated reducing sugar quantitation assay.

Ana Elizabeth Araújo
The enzyme-enriched supernatant of P. pastoris expression was incubated with CMC solution and the release of reducing sugar by the hydrolytic process was quantified in the DNS assay. The supernatant was incubated with the cellulosic substrate for up to 20 minutes. Uninoculated YPD medium and EV-transfected inoculated medium were also detected to discard residual reducing sugars present in the reaction medium and in the basal activity of other P. pastoris hydrolases. TlicBgluc1 activity can enrich...

Supplementary 1: TlicBgli1 cloning and subcloning.

Ana Elizabeth Araújo
(A) Amplification of TlicBgluc1 ORF from cDNA of larvae (1473 bp). (B) Cloning confirmation by restriction analysis with Eco RI and SalI in pGEM®-T Easy vector (3 kb). (C) Subcloning conformation by restriction analysis with of PsTI and SalI in pGAPZα-B protein heterologous-expression vector (3 kb).

Supplementary 1: TlicBgli1 cloning and subcloning.

Ana Elizabeth Araújo
(A) Amplification of TlicBgluc1 ORF from cDNA of larvae (1473 bp). (B) Cloning confirmation by restriction analysis with Eco RI and SalI in pGEM®-T Easy vector (3 kb). (C) Subcloning conformation by restriction analysis with of PsTI and SalI in pGAPZα-B protein heterologous-expression vector (3 kb).

Fig_S1

Sylvain Harquel, Corinne Cian, Pierre-Alain Barraud & Michel Guerraz
Supplemental data for Modulation of alpha waves in sensorimotor cortical networks during
self-motion perception evoked by different visual-vestibular conflicts - JN 00237-2019

Fig_S1

Sylvain Harquel, Corinne Cian, Pierre-Alain Barraud & Michel Guerraz
Supplemental data for Modulation of alpha waves in sensorimotor cortical networks during
self-motion perception evoked by different visual-vestibular conflicts - JN 00237-2019

Lee et al. Burmese Cnemaspis – Supplementary Table

Aryeh Miller, Justin L. Lee, George R. Zug & Daniel G. Mulcahy
Lee et al., Burmese Cnemaspis Supp. Table

Survival, Chemotherapy Treatments, and Health Care Utilization Among Patients with Advanced Small Cell Lung Cancer: An Observational Study

Jinghua He, Changxia Shao, Siu L. Hui, Zuoyi Zhang, Jarod Baker, Paul R. Dexter, Sumesh Kachroo & Fan Jin
Article full text
The article associated with this page has been accepted for online publication and is in the final stages of production. The link to the full text will be made available on this page in the next few days
Provide enhanced digital features for this article
If you are an author of this publication and would like to provide additional enhanced digital features for your article then please contact adisrapidplus@springer.com.

...

Spebrutinib (CC-292) Affects Markers of B-Cell Activation, Chemotaxis and Osteoclasts in Patients with Rheumatoid Arthritis: Results from a Mechanistic Study

Peter H. Schafer, Alan J. Kivitz, Jianglin Ma, Shimon Korish, Donna Sutherland, Li Li, Ada Azaryan, Jolanta Kosek, Mary Adams, Lori Capone, Eun Mi Hur, Douglas R. Hough & Garth E. Ringheim
Provide enhanced digital features for this article
If you are an author of this publication and would like to provide additional enhanced digital features for your article then please contact adisrapidplus@springer.com.

The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are...

Spebrutinib (CC-292) Affects Markers of B-Cell Activation, Chemotaxis and Osteoclasts in Patients with Rheumatoid Arthritis: Results from a Mechanistic Study

Peter H. Schafer, Alan J. Kivitz, Jianglin Ma, Shimon Korish, Donna Sutherland, Li Li, Ada Azaryan, Jolanta Kosek, Mary Adams, Lori Capone, Eun Mi Hur, Douglas R. Hough & Garth E. Ringheim
Provide enhanced digital features for this article
If you are an author of this publication and would like to provide additional enhanced digital features for your article then please contact adisrapidplus@springer.com.

The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are...

Cancer-Specific Loss of Urocortin 3 in Human Renal Cancer

Pouriya Faraj Tabrizi, Anahit Mohebbi Tafrechi, Inga Peters, Faranaz Atschekzei, Markus Antonius Kuczyk, Jürgen Serth & Hossein Tezval
Provide enhanced digital features for this article
If you are an author of this publication and would like to provide additional enhanced digital features for your article then please contact adisrapidplus@springer.com.

The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are...

Registration Year

  • 2019
    27,172

Resource Types

  • Image
    27,172

Data Centers

  • figshare Academic Research System
    27,172