71 Works

Supplementary figure S3. A simple dialysis device for large DNA molecules

Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
Supplementary Figure S3. Agarose gel stained with Sybr Gold with multiple 20 and 40 hour dialysis samples of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S2.

Supplementary figure 1. A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus

Yingxue Li, Zhenzhou Wan, Yihong Hu, Yi Zhou, Qin Chen & Chiyu Zhang
Comparison of the new mismatch-tolerant RT-qPCR with the assays with only Taq (conventional) and high fidelity (Q5) DNA polymerase. A. Detection of RSV mutants; B. Detection HCoV 229E mutants. The amplified products were detected using 2.5% agarose gel electrophoresis. The arrow highlights the specific product. For other details, please see Figure 3.

Supplementary figure S2. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

Supplementary figure 2. Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function

Figshare Future Science Group, Abhijit A Daspute, Xian Yunxuan, Minghua Gu, Yuriko Kobayashi, Sopan Wagh, Archana Panche & Hiroyuki Koyama
Supplementary Figure 1

Supplementary figure 2. Conducting polymer-based multilayer films for instructive biomaterial coatings

Figshare Future Science Group, John G Hardy, Hetian Li, Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S2 - Scatter plot correlating the relationship between the cell orientation (angle relative to the dipping direction of the multilayer films) and length of the cells on the polymer films without and with electrical stimulation. A) PEDOT-PSS-based films. B) PEDOT-S-based films. Black circles represent cells without electrical stimulation, whereas red circles represent cells with electrical stimulation; black and red lines represent the corresponding trend lines.

Supplementary Figure 1. Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach

Yuko Takagi, Hirokazu Nosato, Motomichi Doi, Koji Furukawa & Hidenori Sakanashi
Supplemental Figure1.T. cruzi epimastigote cell count estimation by conventional methods. (A)TC20automated cell counter(Bio-Rad, Hercules, California, USA)was used to count rosette-rich epimastigote, according to the manufacturer’s instruction. Actual cell count was determined by using hemocytometer after breaking apart cell aggregates by pipetting and vortexing. Dottedlinerepresentsthetheoreticallineofperfectcorrelationbetweenactual cell counts and machine-estimated cell counts. (B)Fiji-ImageJ software[1] was used to count the same sample as(A),using threshold adjustment and watershed segmentation functions.(C)Cell Profiler Worm Toolbox[2] was used to count the same...

Supplementary figure S4. A simple dialysis device for large DNA molecules

Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
Supplementary Figure S4. Agarose gel stained with Sybr Gold with single samples of 10, 20, 30 and 40 hour dialysis of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S3.

Supplementary figure 1. Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function

Figshare Future Science Group, Abhijit A Daspute, Xian Yunxuan, Minghua Gu, Yuriko Kobayashi, Sopan Wagh, Archana Panche & Hiroyuki Koyama
Supplementary Figure 1

Supplementary figure S6. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Figure S6. The effect of PEG-rGO on DNA damage. CV curves of 500 µM dGMP mixed with 500 µM cisplatin and (a) 0.15 mg/ml, (b) 0.75 mg/ml PEG-rGO at various time points (scan rate: 20 mV/s). (c) Oxidation peak values. (d) Reaction kinetics study of 500 µM dGMP mixed with 500 µM cisplatin, 500 µM Cisplatin+ 0.15 mg/ml PEG-rGO and 500 µM CDDP+ 0.75 mg/ml PEG-rGO over time.
Figure S6 shows that the reaction rate...

Supplementary figure 1. Conducting polymer-based multilayer films for instructive biomaterial coatings

Figshare Future Science Group, John G Hardy, Hetian Li, Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S1 - Experimental setup for dip coating multilayer films using a Gilson 223 Sample Changer converted for use as a dip coater.

Figure S3 Supplementary material for ‘A simple dialysis device for large DNA molecules’

Figshare Future Science Group, Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
The potential of genomic DNA is realized when new modalities are invented that manipulate large DNAs with minimal breakage or loss of sample. Here, we describe a polydimethylsiloxane–polycarbonate membrane device to remove small molecules from a sample while retaining large DNAs. Dialysis rates dramatically change as DNA size in kb (M) increases and DNA dimensions become comparable to pore size, and chain characteristics go from rod-like to Gaussian. Consequently, we describe empirical rates of dialysis,...

Supplementary table S1. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Table S1. Calculations of P and N percentages on Au surface after cisplatin treatment

Supplementary figure 2. A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus

Yingxue Li, Zhenzhou Wan, Yihong Hu, Yi Zhou, Qin Chen & Chiyu Zhang
Maximum likelihood (ML) tree of sequences from four clinical samples. ML tree of RSV-A and RSV-B sequences obtained from four clinical samples (Sp16, Sp19, Sp8 and Sp9) in this study. The reference sequences were retrieved from GenBank.

Supplementary table S1. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Table S1. Calculations of P and N percentages on Au surface after cisplatin treatment

Supplementary figure S2. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

Supplementary Figure 1. Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach

Yuko Takagi, Hirokazu Nosato, Motomichi Doi, Koji Furukawa & Hidenori Sakanashi
Supplemental Figure1.T. cruzi epimastigote cell count estimation by conventional methods. (A)TC20automated cell counter(Bio-Rad, Hercules, California, USA)was used to count rosette-rich epimastigote, according to the manufacturer’s instruction. Actual cell count was determined by using hemocytometer after breaking apart cell aggregates by pipetting and vortexing. Dottedlinerepresentsthetheoreticallineofperfectcorrelationbetweenactual cell counts and machine-estimated cell counts. (B)Fiji-ImageJ software[1] was used to count the same sample as(A),using threshold adjustment and watershed segmentation functions.(C)Cell Profiler Worm Toolbox[2] was used to count the same...

Supplementary figure 1. Fluidic resistance control enables high throughput establishment of mixed species biofilms

Mads Frederik Hansen, Anders Meyer Torp, Jonas Stenløkke Madsen, Henriette Lyng Røder & Mette Burmølle
Viability is unaffected by short time incubation in high viscosity medium. (A) The number of counted CFU/ml of Pseudomonas putida and Paenibacillus amylolyticus is identical after 45 min. of incubation in PBS and high viscosity liquid (50% glycerol). (B) Similarly, the viability of Xanthomonas retroflexus was unaffected by incubation in high viscosity liquid and did not change over a short time period (N=3, Error bars represent Standard Error (SE)).

Supplementary Figure S2. High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures

Juliette Ohan, Benjamin Pellea, Pulak Nath, J.-H. Huang, Blake Hovde, Momchilo Vuyisich, Armand E.K.Dichosa & Shawn R. Starkenburg
Schematic for cultivation chamber. The cultivation chamber was fabricated and sterilized as described for the microfluidic droplet generator chip (Figure 2 legend). The microfluidic cultivation chamber was used to microscopically visualize droplets without disturbing them or sacrificing viability, as well as providing an even distribution of light.

Supplementary figure S4. A simple dialysis device for large DNA molecules

Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
Supplementary Figure S4. Agarose gel stained with Sybr Gold with single samples of 10, 20, 30 and 40 hour dialysis of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S3.

Registration Year

  • 2019
    71

Resource Types

  • Image
    71

Data Centers

  • Future Science Group
    71