83 Works

Supplementary figure 1. Conducting polymer-based multilayer films for instructive biomaterial coatings

John G Hardy, Hetian Li, Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S1 - Experimental setup for dip coating multilayer films using a Gilson 223 Sample Changer converted for use as a dip coater.

Supplementary figure 3. Conducting polymer-based multilayer films for instructive biomaterial coatings

John G Hardy, Hetian Li, Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S3 HUMAN MESENCHYMAL STEM CELL ADHESION STUDIES HMSCs were supplied by Lonza (Walkersville, MD). Samples were prepared as described above. After sterilization, the samples were incubated for 30 minutes in 24 well plates containing HMSC growth medium that was composed of: high glucose Dulbecco’s Modified Eagle Medium (DMEM, 440 mL); fetal bovine serum (50 mL); antibiotic-antimycotic (5 mL); non-essential amino acids (5 mL), and 2 ng mL-1 basic fibroblast growth factor. Medium was...

Supplementary table S1. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Table S1. Calculations of P and N percentages on Au surface after cisplatin treatment

Supplementary figure S1. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Supplementary figure S1. The effect of excessive TMPD on cisplatin- induced DNA base damage.

Supplementary table 1. Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach

Yuko Takagi, Hirokazu Nosato, Motomichi Doi, Koji Furukawa & Hidenori Sakanashi
Supplemental Table 1. Number of parasites observed in the field of view in each microscopy video images.

Supplementary figure 1. Fluidic resistance control enables high throughput establishment of mixed species biofilms

Mads Frederik Hansen, Anders Meyer Torp, Jonas Stenløkke Madsen, Henriette Lyng Røder & Mette Burmølle
Viability is unaffected by short time incubation in high viscosity medium. (A) The number of counted CFU/ml of Pseudomonas putida and Paenibacillus amylolyticus is identical after 45 min. of incubation in PBS and high viscosity liquid (50% glycerol). (B) Similarly, the viability of Xanthomonas retroflexus was unaffected by incubation in high viscosity liquid and did not change over a short time period (N=3, Error bars represent Standard Error (SE)).

Supplementary figure 2. Conducting polymer-based multilayer films for instructive biomaterial coatings

John G Hardy, Hetian Li, Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S2 - Scatter plot correlating the relationship between the cell orientation (angle relative to the dipping direction of the multilayer films) and length of the cells on the polymer films without and with electrical stimulation. A) PEDOT-PSS-based films. B) PEDOT-S-based films. Black circles represent cells without electrical stimulation, whereas red circles represent cells with electrical stimulation; black and red lines represent the corresponding trend lines.

Supplementary figure S1. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Supplementary figure S1. The effect of excessive TMPD on cisplatin- induced DNA base damage.

Supplementary Figure 1. Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis

Qingdong Guan, Richard Warrington, Sem Moreno, Gefei Qing, Carolyn Weiss & Zhikang Peng
The position of human corresponding vaccine A and D peptides in human IL-18. Green represents Vaccine A peptide; Brown represents Vaccine D peptide.

Supplementary figure S4. A simple dialysis device for large DNA molecules

Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
Supplementary Figure S4. Agarose gel stained with Sybr Gold with single samples of 10, 20, 30 and 40 hour dialysis of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S3.

Supplementary Figure S3. High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures

Juliette Ohan, Benjamin Pellea, Pulak Nath, J.-H. Huang, Blake Hovde, Momchilo Vuyisich, Armand E.K.Dichosa & Shawn R. Starkenburg
Post-cultivation targeted FACS enrichment of abGMDs. Control population of cultivated aGMDs (A) and experimental cultivated population of abGMDs (D) using FSC (x-axis; linear scale) versus SSC (y-axis; log scale). (E) Cultivated abGMDs exhibiting chlorophyll autofluorescence higher than that of the cultivated control aGMDs shown in (B) using a 650/50 nm filter (x-axis at logarithmic scale) versus 530/40 nm filter (y-axis at logarithmic scale). (F) Gated selection of cultivated abGMDs exhibiting higher FSC (to distinguish from...

Supplementary Figure 1. Activation of TAp73 and inhibition of TrxR by Verteporfin for improved cancer therapy in TP53 mutant pancreatic tumors: supplementary material

Pilar Acedo, Aristi Fernandes & Joanna Zawacka-Pankau
Supplementary Figure 1. PpIX and BPD induce ROS generation.(a) PpIX and BPD generated ROS in Panc1 cells as assessed by HE staining. Pre-treatment with 4 μM resveratrol reverted the effect of these compounds. Representative histograms of three independent experiments are shown.(b) Neither, PpIX nor BPD induced ROS in non-transformed HPDE cells (DCFDA staining). H2O2 treatment was used as positive control of ROS induction. The representative histograms are shown (n=3).

Supplementary Figure 1: Staining kinetics of PI and Hoechst in 3D organoids.

Stefanie Mueller, Konstantin J. Bode, Matthias Schweinlin, Marco Metzger & Thomas Brunner
Intact (A) and damaged (B) organoids were stained with PI&Hoechst. Subsequently, brightfield (BF) and fluorescence microscopic images were taken 5, 10 ,15 ,30, and 60 minutes after the dyes were added to culturing medium. (scale bar = 100 µm)

Supplementary figure S6. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Figure S6. The effect of PEG-rGO on DNA damage. CV curves of 500 µM dGMP mixed with 500 µM cisplatin and (a) 0.15 mg/ml, (b) 0.75 mg/ml PEG-rGO at various time points (scan rate: 20 mV/s). (c) Oxidation peak values. (d) Reaction kinetics study of 500 µM dGMP mixed with 500 µM cisplatin, 500 µM Cisplatin+ 0.15 mg/ml PEG-rGO and 500 µM CDDP+ 0.75 mg/ml PEG-rGO over time.
Figure S6 shows that the reaction rate...

Supplementary Figure S2. High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures

Juliette Ohan, Benjamin Pellea, Pulak Nath, J.-H. Huang, Blake Hovde, Momchilo Vuyisich, Armand E.K.Dichosa & Shawn R. Starkenburg
Schematic for cultivation chamber. The cultivation chamber was fabricated and sterilized as described for the microfluidic droplet generator chip (Figure 2 legend). The microfluidic cultivation chamber was used to microscopically visualize droplets without disturbing them or sacrificing viability, as well as providing an even distribution of light.

Figure S2 Supplementary material for ‘A simple dialysis device for large DNA molecules’

Figshare Future Science Group, Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
Abstract The potential of genomic DNA is realized when new modalities are invented that manipulate large DNAs with minimal breakage or loss of sample. Here, we describe a polydimethylsiloxane–polycarbonate membrane device to remove small molecules from a sample while retaining large DNAs. Dialysis rates dramatically change as DNA size in kb (M) increases and DNA dimensions become comparable to pore size, and chain characteristics go from rod-like to Gaussian. Consequently, we describe empirical rates of...

Supplementary figure 1. A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus

Yingxue Li, Zhenzhou Wan, Yihong Hu, Yi Zhou, Qin Chen & Chiyu Zhang
Comparison of the new mismatch-tolerant RT-qPCR with the assays with only Taq (conventional) and high fidelity (Q5) DNA polymerase. A. Detection of RSV mutants; B. Detection HCoV 229E mutants. The amplified products were detected using 2.5% agarose gel electrophoresis. The arrow highlights the specific product. For other details, please see Figure 3.

Supplementary figure 2. A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus

Yingxue Li, Zhenzhou Wan, Yihong Hu, Yi Zhou, Qin Chen & Chiyu Zhang
Maximum likelihood (ML) tree of sequences from four clinical samples. ML tree of RSV-A and RSV-B sequences obtained from four clinical samples (Sp16, Sp19, Sp8 and Sp9) in this study. The reference sequences were retrieved from GenBank.

Supplementary figure S2. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu & Xiaowu (Shirley) Tang
Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

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