The current investigation reports the synthesis of N-CDs using glucosamine, ascorbic acid, and ethylenediamine precursors by a simple hydrothermal technique. The formation of N-CDs was proved by various characterization techniques such as X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Fourier-Transform Infrared Spectrophotometer (FT-IR). The optical properties were investigated by fluorescence and UV-vis spectrophotometer. Also, N-CDs showed high selectivity in detecting the MTZ compared to several other analytes. However, the metronidazole...

The development of safe and effective vaccines to respond to COVID-19 pandemic/endemic remains a priority. We developed a novel subunit protein-peptide COVID-19 vaccine candidate (UB-612) composed of: (i) receptor binding domain of SARS-CoV-2 spike protein fused to a modified single-chain human IgG1 Fc; (ii) five synthetic peptides incorporating conserved helper and cytotoxic T lymphocyte (Th/CTL) epitopes derived from SARS-CoV-2 structural proteins (three from S2 subunit, one from membrane and one from nucleocapsid), and one universal...

Bats are reservoir hosts for various zoonotic viruses with pandemic potential in humans and livestock. In vitro systems for studying bat host-pathogen interactions are of significant interest. Here, we establish protocols to generate bat airway organoids (AOs) and airway epithelial cells differentiated at the air-liquid interface (ALI-AECs) from tracheal tissues of the cave-nectar bat Eonycteris spelaea. In particular, we describe steps which enable laboratories that do not have access to live bats to perform extended...

We identified relapsing fever (RF) Borrelia in 1.45% (145/10426) of the ticks and 1.40% (40/2850) of the wild mammals in a field investigation in China. Three RF Borrelia species, including human-pathogenic Borrelia miyamotoi, Borrelia persica and unclassified Babesia sp. were determined. Main species determined from ticks was B. miyamotoi (44.14%), followed by the unclassified Borrelia sp. (42.76%), and Borrelia theileri (13.10%). In wild mammals, main species found was B. persica (57.50%), followed by the unclassified...

Dichlorodiphenyltrichloroethane (DDT) is well known for its harmful effects and has been officially banned as a pesticide around the world. However, DDT pollution still exists in natural environments in China because DDT degrade very slowly. In this study, 60 soil samples were collected from Cixi, Zhejiang Province, and 1) DDT and its metabolites were determined, 2) Health risk was estimated by using various indices. The results showed that the detection rate of DDT in soil...

In this work, a PAMAM@SiO2 demulsifier was synthesized by the reaction of a hyperbranched poly (amido amine) (PAMAM) and KH570 modified SiO2. The structure, morphology and composition of PAMAM@SiO2 were characterized by FT-IR, SEM and EDS. The effect of the concentration of PAMAM@SiO2, salinity and temperature on the demulsification performance was systematically studied. The results showed that the transmittance of aqueous phase after demulsification could reach as high as 92.1% at optimal conditions, and corresponding...

Introduction: Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes. Methods: The postpartum placental tissue of pregnant women with ICP were chosen as the...

Introduction: Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes. Methods: The postpartum placental tissue of pregnant women with ICP were chosen as the...

The effects of different soil properties on hydrology and nitrogen removal were studied in a simulated bioretention system. Soil capacity of permeability and water retention, changes in the soil environment, leachate concentrations at the surface and bottom layers, quantification of N removal from soil, microorganism and plant by 15N isotope tracer technique, and functional genes abundance at different depths were evaluated. The results showed that shallow root plants, soil compaction, and low organic matter content...

Flue gases emitted from the industries and other emission sources are considered the main atmospheric issues. The main Flue gases emitted are sulfur oxides SOX and Nitrogen oxides NOX. The study was about methods of removing SOX and NOX from emitted gases and the possibility of deriving useful byproducts. The process for removing was investigated using different absorbers, process conditions, and phosphorus allotropes. The yellow phosphorus (P4) was applied for removal accompanied by Potash. The...

The effects of different soil properties on hydrology and nitrogen removal were studied in a simulated bioretention system. Soil capacity of permeability and water retention, changes in the soil environment, leachate concentrations at the surface and bottom layers, quantification of N removal from soil, microorganism and plant by 15N isotope tracer technique, and functional genes abundance at different depths were evaluated. The results showed that shallow root plants, soil compaction, and low organic matter content...

Waning of neutralizing titers along with decline of protection efficacy after the second dose of COVID-19 vaccines was observed, including China-made inactivated vaccines. Efficacy of a heterologous boosting using one dose of a recombinant SARS-CoV-2 fusion protein vaccine (V-01) in inactivated vaccine-primed population was studied, aimed to restore the immunity. A randomized, double-blind and placebo-controlled phase Ⅲ trial was conducted in healthy people aged 18 years or older in Pakistan and Malaysia. Each eligible participant...

Introduction: Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes. Methods: The postpartum placental tissue of pregnant women with ICP were chosen as the...

Replicating SARS-CoV-2 has been shown to degrade HLA class I on target cells to evade the cytotoxic T-cell (CTL) response. HLA-I downregulation can be sensed by NK cells to unleash killer cell immunoglobulin-like receptor (KIR)-mediated self-inhibition by the cognate HLA-I ligands. Here, we investigated the impact of HLA and KIR genotypes and HLA-KIR combinations on COVID-19 outcome. We found that the peptide affinities of HLA alleles were not correlated with COVID-19 severity. The predicted poor...

While some evidence of an effect of radiation exposure on respiratory disease at low dose levels has now emerged, there is heterogeneity in the risks between different studies and countries. In this paper, we aim to show the effect of radiation on three different sub-types of respiratory disease mortality through the analysis of the NRRW cohort in UK. The NRRW cohort consisted of 174,541 radiation workers. Doses to the surface of the body were monitored...

In this work, a PAMAM@SiO2 demulsifier was synthesized by the reaction of a hyperbranched poly (amido amine) (PAMAM) and KH570 modified SiO2. The structure, morphology and composition of PAMAM@SiO2 were characterized by FT-IR, SEM and EDS. The effect of the concentration of PAMAM@SiO2, salinity and temperature on the demulsification performance was systematically studied. The results showed that the transmittance of aqueous phase after demulsification could reach as high as 92.1% at optimal conditions, and corresponding...

Lung adenocarcinoma (LUAD) is one of the most common causes of cancer death in men. BUB1B (BUB1 mitotic checkpoint serine/threonine kinase B) has been reported to contribute to the initiation and development of several cancers. Here, we aimed to explore the potential role of BUB1B in LUAD. We found BUB1B was upregulated in LUAD, suggesting its potential role as a biomarker for LUAD diagnosis. Significantly, LUAD patients with high BUB1B expression had a shorter survival...

Introduction: Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes. Methods: The postpartum placental tissue of pregnant women with ICP were chosen as the...

Autism spectrum disorder (ASD) is one of the common neurodevelopmental disorders in children. Its etiology and pathogenesis are poorly understood. Previous studies suggest potential changes in the complement and coagulation pathways in individuals with ASD.The etiology and pathogenesis have not been clarified. Studies have shown that complement and coagulation pathway were related to ASD. In this study, using multiple reaction monitoring proteomic technology, 16 of the 33 proteins involved in this pathway were identified as...

Autism spectrum disorder (ASD) is one of the common neurodevelopmental disorders in children. Its etiology and pathogenesis are poorly understood. Previous studies suggest potential changes in the complement and coagulation pathways in individuals with ASD.The etiology and pathogenesis have not been clarified. Studies have shown that complement and coagulation pathway were related to ASD. In this study, using multiple reaction monitoring proteomic technology, 16 of the 33 proteins involved in this pathway were identified as...