Angiopoietin-like protein 8, molecular cloning and regulating lipid accumulation in goat intramuscular preadipocytes
Wei Huang, Yaqiu Lin, Hua Xiang, Jiangjiang Zhu & Yong Wang
This study aimed to clone the full-length open reading frame (ORF) of goat ANGPTL8 gene sequence, reveal its molecular and expression characteristics, and explore its effect on the differentiation of goat intramuscular preadipocytes. The full-length ORF sequence of goat ANGPTL8 gene was cloned by RT-PCR technology, and bioinformatics analysis was performed by related biological software. RT-qPCR was used to detect the expression of ANGPTL8 mRNA in goat tissues. Further use of RNA interference to study...
Affiliations
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Affiliated Hospital of Southwest Medical University1
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Chinese Academy of Tropical Agricultural Sciences1
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North West Agriculture and Forestry University1
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Sichuan University1
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Fudan University1
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Chongqing Medical University1
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University of Cincinnati1
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Institute of Subtropical Agriculture1
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University of Delaware1
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Washington University in St. Louis1