22 Works

Synthesis and evaluation of highly releasable and structurally stable antibody-SN-38-conjugates

Lianqi Liu, Fei Xie, Dian Xiao, Xin Xu, Zheng Su, Yanming Wang, Shiyong Fan, Xinbo Zhou & Song Li
Camptothecins, traditional chemotherapy drugs, have been clinically used in antibody-drug conjugates (ADCs), which refreshes the recognition that ADCs preferably incorporate highly potent payloads. However, SN-38, active metabolite of irinotecan from camptothecins, tended to be incorporated into ADCs with an unstable acid sensitive bond, not with the widely used Cathepsin B (CTSB) sensitive bond, which may pose the risk of off-target. Herein, we reported a novel strategy to construct highly releasable and structurally stable SN-38-conjugates, in...

CircSUCO promotes proliferation and differentiation of chicken skeletal muscle satellite cells via sponging miR-15

Yongtong Tian, Xiaoxu Shen, Jing Zhao, Yuanhang Wei, Shunshun Han & Huadong Yin
1. In a previous high-throughput sequencing study, a novel circular RNA (circRNA) generated from a SUN domain containing ossification factor (SUCO) gene transcript (circSUCO) was differentially expressed during the embryonic muscle development. This study aimed to further explore the effect of circSUCO on chicken skeletal muscle development. 2. The experiment analysed the expression patterns of circSUCO in Tianfu broilers and clarified its function in the chicken skeletal muscle satellite cells (SMSC) after circSUCO knockdown. The...

Complete genome sequencing and comparative genomic analyses of a new spotted-fever Rickettsia heilongjiangensis strain B8

Maozhang He, Lifeng Zhang, Haoran Hu, Xiaohan Liu, Cong Zhang, Yu Xin, Boyu Liu, Zhen Chen, Kehan Xu & Yan Liu
Rickettsia heilongjiangensis, a tick-borne obligate intracellular bacterium and causative agent of spotted fever in China, has attracted increasing concern regarding its capability in causing human rickettsiosis. Here, we conducted a genomic analysis of a new Rickettsia heilongjiangensis strain B8 (B8) isolated from the serum of a patient who had been bitten by a Haemaphysalis longicornis tick in Anhui Province, China. The present study sought to identify exclusive genes that might be associated with the pathogenicity...

Adaptive parameter of standard deviation enhances the power of noninvasive prenatal screens

Li Qu, Qixi Wu, Xiaoke Yang, Weiwei Wang, Bing Xu, Guangyu Shan, Lin Liang, Shaowei Wang & Jingbo Zhang
Traditional Z-test methods during noninvasive prenatal screens (NIPS) use the fixed parameter of standard deviation (SD), which ignores the influence of actual sequencing read counts of a sample on the results. The aim of this study is to eliminate the influence of the sequencing depth of individual samples on the results and enhance the power of NIPS. In this study, we propose an improved NIPS method, which calculates the SD in the Z-score process adaptively...

Clinical prognostic risk analysis and progression factor exploration of primary breast lymphoma

Jili Deng, Lan Mi, Xiaopei Wang, Jun Zhu, Chen Zhang & Yuqin Song
: A primary breast lymphomais a rare form of extranodal lymphoma type. We aimed to analyze prognosticriskfactors and explore relapse factors in primary breast diffuse large B cell lymphoma (PB-DLBCL). : From November 2003 to September 2020, sixty-three patients from two medical centers newly diagnosed with PB-DLBCL patients were analyzed retrospectively. : The median age was 52, and >50% of patients were post-menopausal. The international prognostics index (IPI) (0–1) was mainlyin the low-risk group (84%),...

Corticosteroid prevents COVID-19 progression within its therapeutic window: a multicenter, proof-of-concept, observational study.

Yang Li, Xian Zhou, Tao Li, Shiji Chan, Yiqi Yu, Jing-Wen Ai, Haocheng Zhang, Feng Sun, Qiran Zhang, Lei Zhu, Lingyun Shao, Bin Xu & Wenhong Zhang
Background: Critically ill patients with COVID-19 are of grave concern. Those patients usually underwent a stage of excessive inflammation before developing acute respiratory distress syndrome. In this study, we test the hypothesis that short-term, low-to-moderate-dose corticosteroids would benefit patients when used in the early phase of excessive inflammation namely, the therapeutic window. Methods: Among a Shanghai cohort and a validation cohort, we enrolled COVID-19 patients showing marked radiographic progression. Short-term, low-to-moderate-dose corticosteroids were considered for...

Cloning, homology modeling, heterologous expression and bioinformatic analysis of Ure2pA glutathione S-transferase gene from white rot fungus Trametes gibbosa

Jian Zhang, Yujie Chi, Shuxuan Li, Xinzhi Gu & Yi Ye
Glutathione S-transferases (GSTs) are a class of enzymes with detoxification function. GSTs can be used for the degradation of toxic aromatic compounds, such as dyes. In order to promote their application and cost-effective dye degradation and to understand their structure and function more deeply, we cloned and expressed a gst2 gene from Trametes gibbosa—a white rot fungus. T.gibbosa gst2 has a 672-bp open reading frame (ORF) and encodes 223 amino acids. The recombinant Tg-gst2 in...

Neutralization of Omicron sublineages and Deltacron SARS-CoV-2 by 3 doses of BNT162b2 vaccine or BA.1 infection

Chaitanya Kurhade, Jing Zou, Hongjie Xia, Mingru Liu, Qi Yang, Mark Cutler, David Cooper, Alexander Muik, Ugur Sahin, Kathrin U. Jansen, Ping Ren, Xuping Xie, Kena A. Swanson & Pei–Yong Shi
Distinct SARS-CoV-2 Omicron sublineages have evolved showing increased fitness and immune evasion than the original Omicron variant BA.1. Here we report the neutralization activity of sera from BNT162b2 vaccinated individuals or unimmunized Omicron BA.1-infected individuals against Omicron sublineages and “Deltacron” variant (XD). BNT162b2 post-dose 3 immune sera neutralized USA-WA1/2020, Omicron BA.1-, BA.2-, BA.2.12.1-, BA.3-, BA.4/5-, and XD-spike SARS-CoV-2s with geometric mean titers (GMTs) of 1335, 393, 298, 315, 216, 103, and 301, respectively; thus, BA.4/5...

Molecular epidemiology and clinical features of hand, foot and mouth disease requiring hospitalization after the use of enterovirus A71 inactivated vaccine in Chengdu, China, 2017-2022: a descriptive study

Xiaoxia Duan, Chaoyong Zhang, Xiao Wang, Xueling Ren, Hongxia Peng, Xueqin Tang, Liangzhi Zhang, Zhenhua Chen, Yan Ye, Mengmou Zheng, Wanzhen Zhong, Xiyue Chen, Yilan Zeng, Ping Yuan & Lu Long
Three inactivated enterovirus A71 (EV-A71) vaccines have been widely vaccinated among children in the targeted age group in mainland China since mid-2016. However, comprehensive virological surveillance of hand, foot and mouth disease (HFMD) over multiple years after the use of EV-A71 vaccines has rarely been conducted. Using long-term data extracted from the Public Health and Clinical Center of Chengdu, we described the clinical, aetiological, and epidemiological characteristics of HFMD inpatients after the use of EV-A71...

Batch-to-batch consistency trial of an adenovirus type-5 vector-based COVID-19 vaccine in adults aged 18 years and above

Zhuo-Pei Li, Yun-Feng Shi, Li-Hua Hou, Peng-Fei Jin, Shi-Hua Ma, Hong-Xing Pan, Jin-Long Zhang, Yong-Mei Shan, Hai-Tao Huang, Shi-Po Wu, Pan Du, Xue Wang, Li-Li Wang, Rui-Jie Wang, Ying Wang, Xue-Wen Wang, Feng-Cai Zhu & Jing-Xin Li
The demonstration of batch-to-batch consistency is indispensable for quality control of vaccines. We did a randomized, double-blind, parallel-controlled trial to evaluate the immunogenicity consistency of one shot of Ad5-nCoV in healthy adults who had not received any COVID-19 vaccine before. All eligible participants were randomly assigned equally to receive one of three consecutive batches of Ad5-nCoV (5×1010 viral particles/vial, 0.5 mL). The primary endpoint was geometric mean titers (GMTs) of serum SARS-CoV-2 receptor binding domain...

Complete genome sequencing and comparative genomic analyses of a new spotted-fever Rickettsia heilongjiangensis strain B8

Maozhang He, Lifeng Zhang, Haoran Hu, Xiaohan Liu, Cong Zhang, Yu Xin, Boyu Liu, Zhen Chen, Kehan Xu & Yan Liu
Rickettsia heilongjiangensis, a tick-borne obligate intracellular bacterium and causative agent of spotted fever in China, has attracted increasing concern regarding its capability in causing human rickettsiosis. Here, we conducted a genomic analysis of a new Rickettsia heilongjiangensis strain B8 (B8) isolated from the serum of a patient who had been bitten by a Haemaphysalis longicornis tick in Anhui Province, China. The present study sought to identify exclusive genes that might be associated with the pathogenicity...

Study on inhibition effect and mechanism of NH4H2PO4 on explosion of titanium powder

Wenjiao Dai, Xiangbao Meng, Shunxiang Yao, Jiqing Liu, Zhifeng Wang, Yang Liu, Panpan Yang & Fang Li
In order to effectively inhibit the explosion of titanium powder, this study used the Hartmann tube experimental system and the 20 L explosion tank experimental system to carry out flame propagation and explosion overpressure experiments, respectively, and the effect of NH4H2PO4 on the explosion suppression of titanium powder was studied. The results show that when the addition of NH4H2PO4 powder is 50%, the flame propagation velocity and explosion pressure can be reduced to the minimum,...

Study on inhibition effect and mechanism of NH4H2PO4 on explosion of titanium powder

Wenjiao Dai, Xiangbao Meng, Shunxiang Yao, Jiqing Liu, Zhifeng Wang, Yang Liu, Panpan Yang & Fang Li
In order to effectively inhibit the explosion of titanium powder, this study used the Hartmann tube experimental system and the 20 L explosion tank experimental system to carry out flame propagation and explosion overpressure experiments, respectively, and the effect of NH4H2PO4 on the explosion suppression of titanium powder was studied. The results show that when the addition of NH4H2PO4 powder is 50%, the flame propagation velocity and explosion pressure can be reduced to the minimum,...

Clinical prognostic risk analysis and progression factor exploration of primary breast lymphoma

Jili Deng, Lan Mi, Xiaopei Wang, Jun Zhu, Chen Zhang & Yuqin Song
: A primary breast lymphomais a rare form of extranodal lymphoma type. We aimed to analyze prognosticriskfactors and explore relapse factors in primary breast diffuse large B cell lymphoma (PB-DLBCL). : From November 2003 to September 2020, sixty-three patients from two medical centers newly diagnosed with PB-DLBCL patients were analyzed retrospectively. : The median age was 52, and >50% of patients were post-menopausal. The international prognostics index (IPI) (0–1) was mainlyin the low-risk group (84%),...

Synthesis and evaluation of highly releasable and structurally stable antibody-SN-38-conjugates

Lianqi Liu, Fei Xie, Dian Xiao, Xin Xu, Zheng Su, Yanming Wang, Shiyong Fan, Xinbo Zhou & Song Li
Camptothecins, traditional chemotherapy drugs, have been clinically used in antibody-drug conjugates (ADCs), which refreshes the recognition that ADCs preferably incorporate highly potent payloads. However, SN-38, active metabolite of irinotecan from camptothecins, tended to be incorporated into ADCs with an unstable acid sensitive bond, not with the widely used Cathepsin B (CTSB) sensitive bond, which may pose the risk of off-target. Herein, we reported a novel strategy to construct highly releasable and structurally stable SN-38-conjugates, in...

Aggregation-induced emission tetraphenylethylene derivative as optical sensor for ammonia detection

Shoubin Zhang, Di Zhao, Liuyi Xiong, Yufeng Hu, Zhidong Lou, Yanbing Hou, Feng Teng & Qiuhong Cui
We propose a general approach for the achievement of optical sensors, where ammonia can be rapidly identified and detected through visible fluorescence changes. Here, a tetraphenylethylene derivative with carboxyl groups named TPE-2CA (4,4ʹ-(1,2-Diphenylethene-1,2-diyl) dibenzoyl acid) was applied in the sensor. The AIE (aggregation-induced emission) property of tetraphenylethylene structure provides the samples with excellent luminescent performance in aggregated state. The reaction between carboxyl groups and ammonia causes the shifts on luminescence spectra of the samples. Consequently,...

Efficacy and safety of novel-targeted drugs in the treatment of pulmonary arterial hypertension: a Bayesian network meta-analysis

Wenhai Fu, Wenjun He, Yuexin Li, Yangxiao Chen, Jingyi Liang, Hui Lei, Lin Fu, Yanghang Chen, Ni Ren, Qian Jiang, Yi Shen, Ran Ma, Tao Wang, Xinni Wang, Nuofu Zhang, Dakai Xiao & Chunli Liu
Background: Pulmonary arterial hypertension (PAH) is a severe and fatal clinical syndrome characterized by high blood pressure and vascular remodeling in the pulmonary arterioles, which is also a rapidly progressing disease of the lung vasculature with a poor prognosis. Although PAH medication made great advances in recent years, the efficacy and safety of the medication are unsatisfactory. Therefore, we aimed to update and expand previous studies to explore the efficacy and safety of PAH-targeted medications....

Efficacy and safety of novel-targeted drugs in the treatment of pulmonary arterial hypertension: a Bayesian network meta-analysis

Wenhai Fu, Wenjun He, Yuexin Li, Yangxiao Chen, Jingyi Liang, Hui Lei, Lin Fu, Yanghang Chen, Ni Ren, Qian Jiang, Yi Shen, Ran Ma, Tao Wang, Xinni Wang, Nuofu Zhang, Dakai Xiao & Chunli Liu
Background: Pulmonary arterial hypertension (PAH) is a severe and fatal clinical syndrome characterized by high blood pressure and vascular remodeling in the pulmonary arterioles, which is also a rapidly progressing disease of the lung vasculature with a poor prognosis. Although PAH medication made great advances in recent years, the efficacy and safety of the medication are unsatisfactory. Therefore, we aimed to update and expand previous studies to explore the efficacy and safety of PAH-targeted medications....

Cloning, homology modeling, heterologous expression and bioinformatic analysis of Ure2pA glutathione S-transferase gene from white rot fungus Trametes gibbosa

Jian Zhang, Yujie Chi, Shuxuan Li, Xinzhi Gu & Yi Ye
Glutathione S-transferases (GSTs) are a class of enzymes with detoxification function. GSTs can be used for the degradation of toxic aromatic compounds, such as dyes. In order to promote their application and cost-effective dye degradation and to understand their structure and function more deeply, we cloned and expressed a gst2 gene from Trametes gibbosa—a white rot fungus. T.gibbosa gst2 has a 672-bp open reading frame (ORF) and encodes 223 amino acids. The recombinant Tg-gst2 in...

Efficacy of heterologous boosting against SARS-CoV-2 using a recombinant interferon-armed fusion protein vaccine (V-01): a randomized, double-blind and placebo-controlled phase III trial

Xuan-Yi Wang, Syed Faisal Mahmood, Fang Jin, Wee Kooi Cheah, Muhammad Ahmad, Mian Amjad Sohail, Waheed Ahmad, Vijaya K Suppan, Muneeba Ahsan Sayeed, Shobha Luxmi, Aik-Howe Teo, Li Yuan Lee, Yang-Yang Qi, Rong-Juan Pei, Wei Deng, Zhong-Hui Xu, Jia-Ming Yang, Yan Zhang, Wu-Xiang Guan & Xiong Yu
Waning of neutralizing titers along with decline of protection efficacy after the second dose of COVID-19 vaccines was observed, including China-made inactivated vaccines. Efficacy of a heterologous boosting using one dose of a recombinant SARS-CoV-2 fusion protein vaccine (V-01) in inactivated vaccine-primed population was studied, aimed to restore the immunity. A randomized, double-blind and placebo-controlled phase Ⅲ trial was conducted in healthy people aged 18 years or older in Pakistan and Malaysia. Each eligible participant...

Integrated analysis of long non-coding RNAs and mRNA expression profiles identified potential interactions regulating melanogenesis in chicken skin

Shigang Yu, Gang Wang, Juan Liao, Xuemei Shen & Jia Chen
1. Long non-coding RNAs (lncRNAs) play important roles in various physiological functions. However, the mechanisms underlying the regulation of lncRNAs in melanogenesis remain unclear. To determine the molecular mechanisms involved in the melanogenesis of skin melanogenesis, the present study depicted the expression profiles of lncRNAs and messenger RNAs (mRNAs) in black- (B group) and white- (W group) skinned chickens using RNA sequencing. 2. In total, 373 differentially expressed lncRNAs (DELs; 203 up-regulated and 170 down-regulated)...

MERS-CoV nsp1 regulates autophagic flux via mTOR signaling and dysfunctional lysosomes

Yujie Feng, Zhaoyi Pan, Zhihui Wang, Zhengyang Lei, Songge Yang, Huajun Zhao, Xueyao Wang, Yating Yu, Qiuju Han & Jian Zhang
Autophagy, a cellular surveillance mechanism, plays an important role in combating invading pathogens. However, viruses have evolved various strategies to disrupt autophagy and even hijack it for replication and release. Here, we demonstrated that Middle East respiratory syndrome coronavirus (MERS-CoV) non-structural protein 1(nsp1) induces autophagy but inhibits autophagic activity. MERS-CoV nsp1 expression increased ROS and reduced ATP levels in cells, which activated AMPK and inhibited the mTOR signaling pathway, resulting in autophagy induction. Meanwhile, as...

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  • Henan University
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  • Fudan University
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