48 Works

Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK

Xuelin Han, Xueting Su, Zhiqian Li, Yanxi Liu, Shuo Wang, Miao Zhu, Changjian Zhang, Fan Yang, Jingya Zhao, Xianping Li, Fangyan Chen & Li Han
Complement receptor 3 (CD11b/CD18) is an important receptor that mediates adhesion, phagocytosis and chemotaxis in various immunocytes. The conidia of the medically-important pathogenic fungus, Aspergillus fumigatus can be internalized into alveolar epithelial cells to disseminate its infection in immunocompromised host; however, the role of CR3 in this process is poorly understood. In the present study, we investigated the potential role of CR3 on A. fumigatus internalization into type II alveolar epithelial cells and its effect...

Angiopoietin-like protein 8, molecular cloning and regulating lipid accumulation in goat intramuscular preadipocytes

Wei Huang, Yaqiu Lin, Hua Xiang, Jiangjiang Zhu & Yong Wang
This study aimed to clone the full-length open reading frame (ORF) of goat ANGPTL8 gene sequence, reveal its molecular and expression characteristics, and explore its effect on the differentiation of goat intramuscular preadipocytes. The full-length ORF sequence of goat ANGPTL8 gene was cloned by RT-PCR technology, and bioinformatics analysis was performed by related biological software. RT-qPCR was used to detect the expression of ANGPTL8 mRNA in goat tissues. Further use of RNA interference to study...

BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) Promotes Lung Adenocarcinoma by Interacting with Zinc Finger Protein ZNF143 and Regulating Glycolysis

Xiaolei Zhou, Yanli Yuan, Hongping Kuang, Bingxiang Tang, Hui Zhang & Manlin Zhang
Lung adenocarcinoma (LUAD) is one of the most common causes of cancer death in men. BUB1B (BUB1 mitotic checkpoint serine/threonine kinase B) has been reported to contribute to the initiation and development of several cancers. Here, we aimed to explore the potential role of BUB1B in LUAD. We found BUB1B was upregulated in LUAD, suggesting its potential role as a biomarker for LUAD diagnosis. Significantly, LUAD patients with high BUB1B expression had a shorter survival...

An analysis of the optimal intrusion force of the maxillary central incisor with root horizontal resorption using the finite element method and curve fitting

Xin Zhang, Min-Qi Li, Jie Guo, Heng-Wei Yang, Jian Yu & Guo-Ju Li
There are no studies on the optimal intrusion force in orthodontic patients with the existing root resorption (RR). The study aimed to analyze the optimal intrusion force for central incisors with existing horizontal root resorption using the finite element method (FEM). We calculated the optimal intrusion force using the finite element method and curve fitting. We found that with the increase of the maxillary central incisor’s root horizontal resorption length, the optimal intrusion force interval’s...

Omicron infections profile and vaccination status among 1881 liver transplant recipients: a multi-center retrospective cohort

Ensi Ma, Jingwen Ai, Yi Zhang, Jianming Zheng, Xiaogang Gao, Junming Xu, Hao Yin, Zhiren Fu, Hao Xing, Li Li, Liying Sun, Heyu Huang, Quanbao Zhang, Linlin Xu, Yanting Jin, Rui Chen, Guoyue Lv, Zhijun Zhu, Wenhong Zhang & Zhengxin Wang
A wave of Omicron infections rapidly emerged in China in 2022, but large-scale data concerning the safety profile of vaccines and Coronavirus disease 2019 (COVID-19) infection features in liver transplant (LT) recipients have not been collected. Therefore, the aim of this study was to assess the protectiveness and safety profile of the inactivated vaccines in LT patients against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant infections. A multi-center retrospective study was conducted in...

Speeding up Genetic Programming Based Symbolic Regression Using GPUs

Rui Zhang, Andrew Lensen & Yanan Sun
No description supplied

Booster vaccination is required to elicit and maintain COVID-19 vaccine-induced immunity in SIV-infected macaques

Pingchao Li, Qian Wang, Yizi He, Chenchen Yang, Zhengyuan Zhang, Zijian Liu, Bo Liu, Li Yin, Yilan Cui, Peiyu Hu, Yichu Liu, Pingqian Zheng, Wei Wang, Linbing Qu, Caijun Sun, Suhua Guan, Liqiang Feng & Ling Chen
Prolonged infection and possible evolution of SARS-CoV-2 in patients living with uncontrolled HIV-1 infection highlight the importance of an effective vaccination regimen, yet the immunogenicity of COVID-19 vaccines and predictive immune biomarkers have not been well investigated. Herein, we report that the magnitude and persistence of antibody and cell-mediated immunity (CMI) elicited by an Ad5-vectored COVID-19 vaccine are impaired in SIV-infected macaques with high viral loads (> 105 genome copies per ml plasma, SIVhi) but...

Fracture behavior of high-strength bolts and connections in the whole process of fire

Qiao Chen, Jian Jiang, Wei Chen & Jihong Ye
High-strength bolts have been widely used in steel bolted connections, and fire-induced high temperatures will affect its basic mechanical properties and fracture behavior, leading to failure of the connection and even collapse of the overall structure. Tensile experiments are conducted on Grade 10.9 high-strength bolts to investigate the effect of stress triaxiality and heating history on their mechanical and fracture properties in the whole process of fire. The true stress-strain curves are obtained to determine...

Comparisons on fluid-solid heat transfer simulation for localized fire resistance

Xuanyi Zhou, Jinggang Zhou, Beihua Cong & Wei Wang
A research idea of multi-physics field coupling can be used to solve the structural fire resistance problem. Most of the previous CFD-FEM numerical simulations in structural fire resistance studies apply adiabatic surface temperature (AST) to accurately describe the complex fire analysis boundary conditions, and this methodology belongs to the use of iterative coupling methods to achieve fluid-solid heat transfer coupling. However, there is less application for another direct two-way coupling method. In this paper, a...

A novel SNP of MYO1A gene associated with heat-tolerance in Chinese cattle

Yanhong Cao, Peng Jia, Zhuyue Wu, Mingguang Huang, Shaomei Chen, Jicai Zhang, Bizhi Huang & Chuzhao Lei
With the advent of global climate change, heat-tolerance is becoming more and more important to the sustainability of animal husbandry production systems. Previous studies have shown that MYO1A gene associated with pigmentation may be closely related to heat-tolerance in cattle. In this study, a novel missense mutation (NC_037332.1 g.56390345 A > G) was first detected in MYO1A in 891 individuals of 35 cattle breeds, which transformed the amino acid isoleucine into valine. The purpose of...

Whole-genome resequencing reveals aberrant autosomal SNPs affect chicken feathering rate

Mohan Qiu, Chaowu Yang, Huarui Du, Qingyun Li, Zengrong Zhang, Xia Xiong, Chunlin Yu, Xiaoyan Song, Chenming Hu, Bo Xia, Li Yang, Han Peng, Lan Liu & Xiaosong Jiang
Previous studies have shown that the feather growth rate of chicks is determined by two alleles located on the sex chromosome Z; however, in chicken production, feathering is usually not consistently controlled by the sex chromosome. To identify whether the feathering rate is related to autosomal inheritance, whole-genome resequencing was performed in eight chickens with slow- and fast-feathering rate. A total of 54,984 autosomal single nucleotide polymorphisms (SNPs) were identified, including 393 and 376 exonic...

Removal of harmful algae by Shigella sp. H3 and Alcaligenes sp. H5: algicidal pathways and characteristics

Gang Xue, Xiaonuan Wang, Chenlan Xu, Binxue Song & Hong Chen
Application of algicidal bacteria is a promising technology to control harmful algal blooms (HABs). In this study, algicidal bacteria strains Shigella sp. H3 and Alcaligenes sp. H5 were obtained via two different isolation methods from the same lake water sample, with optimal algicidal efficiencies 96% and 74% against algae mixture. The Shigella sp. H3 and Alcaligenes sp. H5 lysed algae cells through cells-to-cells direct contact and secretion of algicidal metabolites, respectively. The stronger algicidal capability...

Genetic, biological and epidemiological study on a cluster of H9N2 avian influenza virus infections among chickens, a pet cat, and humans at a backyard farm in Guangxi, China

Jing Yang, Jianhua Yan, Cheng Zhang, Shanqin Li, Manhua Yuan, Chunge Zhang, Chenguang Shen, Yang Yang, Lifeng Fu, Guanlong Xu, Weifeng Shi, Zhenghai Ma, Ting Rong Luo & Yuhai Bi
During an investigation in October 2018, two people with diarrhoea, mild abdominal pain, and mild arthralgia symptoms in Guangxi, China, were identified as infected by H9N2 avian influenza virus (AIV). Four H9N2 AIVs were isolated from one of two patients, a pet cat, and a dead chicken (two respective isolates from its lung and kidney tissues) bred by the patients at a backyard farm. Epidemiological investigation indicated that the newly bought chicken died first, and...

A tandem-repeat dimeric RBD protein-based COVID-19 vaccine ZF2001 protects mice and nonhuman primates

Yaling An, Shihua Li, Xiyue Jin, Jian-bao Han, Kun Xu, Senyu Xu, Yuxuan Han, Chuanyu Liu, Tianyi Zheng, Mei Liu, Mi Yang, Tian-zhang Song, Baoying Huang, Li Zhao, Wen Wang, A Ruhan, Yingjie Cheng, Changwei Wu, Enqi Huang, Shilong Yang, Gary Wong, Yuhai Bi, Changwen Ke, Wenjie Tan, Jinghua Yan … & George F. Gao
Safe, efficacious and deployable vaccines are urgently needed to control COVID-19 in the large-scale vaccination campaigns. We report here the preclinical studies of an approved protein subunit vaccine against COVID-19, ZF2001, which contains tandem-repeat dimeric receptor-binding domain (RBD) protein with alum-based adjuvant. We assessed vaccine immunogenicity and efficacy in both mice and non-human primates (NHPs). ZF2001 induced high levels of RBD-binding and SARS-CoV-2 neutralizing antibody in both mice and non-human primates, and elicited balanced TH1/TH2...

Cadomian crust of Eastern Iran: evidence from the Tapeh Tagh granitic gneisses

Habib Mollai, Rahim Dabiri, Habib Allah Torshizian, Georgia Pe-Piper & Wei Wang
Late Neoproterozoic to Early Cambrian (Cadomian) magmatic rocks are abundant in Iran, but their occurrence in east-central Iran is voluminous. This paper reports, for the first time, on the Cadomian rocks of the eastern Iranian region known as Tapeh Tagh and deals with their geochemistry and uranium-lead (U-Pb) zircon ages. The Tapeh Tagh region contains masses of Cadomian meta-igneous rocks (including various types of granitic gneiss) and overlying meta-sedimentary rocks. The granitic gneisses show tectonic...

Comparisons on fluid-solid heat transfer simulation for localized fire resistance

Xuanyi Zhou, Jinggang Zhou, Beihua Cong & Wei Wang
A research idea of multi-physics field coupling can be used to solve the structural fire resistance problem. Most of the previous CFD-FEM numerical simulations in structural fire resistance studies apply adiabatic surface temperature (AST) to accurately describe the complex fire analysis boundary conditions, and this methodology belongs to the use of iterative coupling methods to achieve fluid-solid heat transfer coupling. However, there is less application for another direct two-way coupling method. In this paper, a...

Convective heat transfer coefficient of steel member in localized fire condition

Jinggang Zhou, Xuanyi Zhou, Beihua Cong & Wei Wang
At present, values adopted from specifications are mostly used to define the convective heat transfer coefficient ch in localized fire researches. In this paper, the specific scenario of the steel column surrounded by fire plume is selected as a typical case to explore the surface ch change rules of the steel column. This paper conducts a two-way coupling simulation analysis of the fire and the thermal models by means of CFD codes. First, the validity...

Assessment of structural steel members temperatures in open car park fires: different modelling approaches

Xia Yan, Marion Charlier & Thomas Gernay
Open car parks, as defined based on ventilation conditions, are one of the most common types of car park buildings. For such structures, adopting a performance-based structural fire design based on localised fires is often justified and allowed because the fire does not reach flashover. As this approach requires assessing the temperatures in the structure, this paper focuses on the numerical analysis of the temperatures reached in open car park steel beams under localised fires...

Succession of the microbial communities and metabolic functions in composting or deep burial processing of dead chickens

Zhongzhen Lin, Chaowu Yang, Bo Xia, Mohan Qiu, Han Peng, Xiaosong Jiang, Huarui Du, Qingyun Li, Zengrong Zhang, Yiping Liu & Chunlin Yu
1. This study examined the effects of composting and deep burial techniques on degradation efficiency of dead chickens. Different raw materials (crushed branches or rape straws) and disinfectants (quicklime or bleaching powder) were applied in composting and deep burial process, respectively. The whole process lasted for 90 d in both summer and winter. 2. High throughput sequencing displayed that Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria and Deinococcus-Thermus were the most dominant bacterial phyla during the experiment. The...

Emerging and re-emerging KPC-producing hypervirulent Pseudomonas aeruginosa ST697 and ST463 between 2010 and 2021

Biying Zhang, Xun Xu, Xiaomei Song, Yicheng Wen, Zhichen Zhu, Jingnan Lv, Xiaofang Xie, Liang Chen, Yi-Wei Tang & Hong Du
Carbapenem-resistant Pseudomonas aeruginosa (CRPA) has been a major threat to human health due to its increased morbidity and mortality in clinical settings. Carbapenemase genes are less frequently found in CRPA compared with carbapenem-resistant Enterobacterales, of which carbapenemase producers are common. In this study, we identified 11 blaKPC-2-harboring P. aeruginosa isolates from 139 carbapenemase-insensitive P. aeruginosa isolates collected between 2010 and 2021 in a tertiary hospital in China. Nine isolates belonged to ST697, while the other...

Adjunctive Zoledronate+IL-2 administrations enhance anti-tuberculosis Vγ2Vδ2 T-effector populations, and improve treatment outcome of multidrug-resistant tuberculosis.

Hongbo Shen, Enzhuo Yang, Ming Guo, Rui Yang, Guixian Huang, Ying Peng, Wei Sha, Feifei Wang & Ling Shen
Multidrug-resistant tuberculosis(MDR-TB) is a refractory disease with high mortality rate due to no or few choices of antibiotics. Adjunctive immunotherapy may help improve treatment outcome of MDR-TB. Our decade-long studies demonstrated that phosphoantigen-specific Vγ2Vδ2 T cells play protective roles in immunity against TB. Here, we hypothesized that enhancing protective Vγ2Vδ2 T-effector cells could improve treatment outcome of MDR-TB. To address this, we employed clinically-approved drugs Zoledronate(ZOL) and IL-2 to induce anti-TB Vγ2Vδ2 T-effector cells as...

RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variant

Jianing Yang, Xuejiao Hu, Wenzhuo Wang, Yujing Yang, Xinqiang Zhang, Wei Fang, Lei Zhang, Shan Li & Bing Gu
The highly infectious Delta variant strain of SARS-CoV-2 remains globally dominant and undermines COVID-19 vaccines. Rapid detection of the Delta variant is crucial for the identification and quarantine of infected individuals. In this study, our aim was to design and validate a genotyping RT-LAMP method to detect Delta variants specifically. R203M in the N gene of SARS-CoV-2 was chosen as the Delta variant-specific mutation for genotyping. To target the R203M-harboring region and the conserved sequence...

miR-199-3p Suppresses Cellular Migration and Viability, Promotes Progesterone Production in Goose Ovarian Follicles Before Selection Through Regulating ITGB8 and Other ECM-related Genes

Qin Li, Keshan Zhang, Xianzhi Zhao, Yushi Wang, Jing Li, Youhui Xie, Hang Zhong & Qigui Wang
1. The extracellular matrix (ECM) constitutes the basal lamina and the area between follicular cells. Remodelling the ECM is believed to be a key event in follicular development, especially during selection, and plays an important role in cell migration, survival, and steroidogenesis. miR-199-3p is differentially expressed in the goose granulosa layer during follicular selection and is reported to play a primary role in inhibiting cell migration and invasion. Nevertheless, the effect of miR-199-3p on ovarian...

Creep behavior of Grade 10.9 high-strength bolts under and after fire

Yaoliang Lu, Jian Jiang, Wenyu Cai, Wei Chen & Jihong Ye
As an important component in steel connections, high-strength bolts have a high stress level and may experience creep deformation at high temperatures. This will lead to great deformation and even failure of steel connections, thus affecting progressive collapse resistance of steel structures. This paper experimentally and numerically investigates the creep behavior of 10.9 high-strength bolts under and after fire. The creep strain-time curves at different temperatures and stress levels were obtained using DIC measurement systems....

Impact of vaccination on kinetics of neutralizing antibodies against SARS-CoV-2 by Serum live neutralization test based on a prospective cohort

Liguo Zhu, Naiying Mao, Changhua Yi, Aidibai Simayi, Jialu Feng, Yi Feng, Min He, Songning Ding, Yin Wang, Yan Wang, Mingwei Wei, Jie Hong, Chuchu Li, Hua Tian, Lu Zhou, Jiefu Peng, Shihan Zhang, Ci Song, Hui Jin, Fengcai Zhu, Wenbo Xu, Jun Zhao & Changjun Bao
How much the vaccine contributes to the induction and development of neutralizing antibodies (NAbs) of breakthrough cases relative to those unvaccinated-infected cases is not fully understood. We conducted a prospective cohort study and collected serum samples from 576 individuals who were diagnosed with SARS-CoV-2 Delta strain infection, including 245 breakthrough cases and 331 unvaccinated-infected cases. NAbs were analyzed by live virus microneutralization test and transformation of NAb titer. NAbs titers against SARS-CoV-2 ancestral and Delta...

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  • North West Agriculture and Forestry University
  • Fudan University
  • Zhejiang University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Sun Yat-sen University
  • Southern Medical University
  • Capital Medical University
  • Chinese Academy of Sciences
  • Central South University