42 Works

Novel H5N6 reassortants bearing the clade HA gene of H5N8 virus have been detected in poultry and caused multiple human infections in China

Wenli Gu, Jianzhong Shi, Pengfei Cui, Cheng Yan, Yaping Zhang, Congcong Wang, Yuancheng Zhang, Xin Xing, Xianying Zeng, Liling Liu, Guobin Tian, Yasuo Suzuki, Chengjun Li, Guohua Deng & Hualan Chen
The globally circulating H5N8 avian influenza viruses bearing the clade hemagglutinin (HA) gene are responsible for the loss of more than 33 million domestic poultry since January 2020. Moreover, the H5N8 viruses have reassorted with other avian influenza viruses and formed H5N1, H5N2, H5N3, H5N4, and H5N5 viruses in Europe, Africa, and North America. In this study, we analyzed 15 H5N6 viruses isolated from poultry and seven H5N6 viruses isolated from humans, and found...

Molecular detection and identification of relapsing fever Borrelia in ticks and wild small mammals in China

Xiao-Ai Zhang, Feng Tian, Yue Li, Xiao-Long Zhang, Bao-Gui Jiang, Bao-Cheng Liu, Jing-Tao Zhang, Shen Tian, Heng Ding, Shuang Li, Hao Li, Li-Qun Fang & Wei Liu
We identified relapsing fever (RF) Borrelia in 1.45% (145/10426) of the ticks and 1.40% (40/2850) of the wild mammals in a field investigation in China. Three RF Borrelia species, including human-pathogenic Borrelia miyamotoi, Borrelia persica and unclassified Babesia sp. were determined. Main species determined from ticks was B. miyamotoi (44.14%), followed by the unclassified Borrelia sp. (42.76%), and Borrelia theileri (13.10%). In wild mammals, main species found was B. persica (57.50%), followed by the unclassified...

Complete genome sequencing and comparative genomic analyses of a new spotted-fever Rickettsia heilongjiangensis strain B8

Maozhang He, Lifeng Zhang, Haoran Hu, Xiaohan Liu, Cong Zhang, Yu Xin, Boyu Liu, Zhen Chen, Kehan Xu & Yan Liu
Rickettsia heilongjiangensis, a tick-borne obligate intracellular bacterium and causative agent of spotted fever in China, has attracted increasing concern regarding its capability in causing human rickettsiosis. Here, we conducted a genomic analysis of a new Rickettsia heilongjiangensis strain B8 (B8) isolated from the serum of a patient who had been bitten by a Haemaphysalis longicornis tick in Anhui Province, China. The present study sought to identify exclusive genes that might be associated with the pathogenicity...

Supplementary Table.doc

Bingyan Cao, Meijuan Liu, Yingxian Zhang, JiaJia Chen, Xiaoqiao Li, Chang Su, Wei Yang, Min Liu, Di Wu, Wenjing Li, Xuejun Liang, Qiao Wang, Haiyan Wei & Chunxiu Gong
Supplementary Table 1 Positive rate of different MODY screening criteria Supplementary Table 2 The clinical and genetic characteristics of MODY in two cohorts (gray lines represent the cohort 2)

Epidemiology, evolution, and biological characteristics of H6 avian influenza viruses in China

Xiaohao Xu, Qi Chen, Min Tan, Jia Liu, Xiyan Li, Lei Yang, Yuelong Shu, Dayan Wang & Wenfei Zhu
H6 avian influenza virus (AIV) is one of the most prevalent AIV subtypes in birds globally. To investigate the current situation and characteristics of H6 AIVs circulating in China, we analysed the epidemiology, genetic evolution and pathogenic features of this subtype. During 2000-2021, H6 subtype AIVs spread widely through Southern China and presented high host diversity. On analysing 171 H6 viruses isolated during 2009-2021, dynamic reassortments were observed among H6 and other co-circulating AIV subtypes,...

FBXO34 promotes latent HIV-1 activation by post-transcriptional modulation

Xinyi Yang, Xiaying Zhao, Yuqi Zhu, Jingna Xun, Qin Wen, Hanyu Pan, Jinlong Yang, Jing Wang, Zhimin Liang, Xiaoting Shen, Yue Liang, Qinru Lin, Huitong Liang, Min Li, Jun Chen, Shibo Jiang, Jianqing Xu, Hongzhou Lu & Huanzhang Zhu
Acquired immunodeficiency syndrome (AIDS) cannot be completely cured, mainly due to the existence of a latent HIV-1 reservoir. However, our current understanding of the molecular mechanisms underlying the establishment and maintenance of HIV-1 latent reservoir is not comprehensive. Here, using a genome-wide CRISPR-Cas9 activation library screening, we identified E3 ubiquitin ligase F-box protein 34 (FBXO34) and the substrate of FBXO34, heterogeneous nuclear ribonucleoprotein U (hnRNP U) was identified by affinity purification mass spectrometry, as new...

Distribution, sources and health risk assessment of DDT and its metabolites in agricultural soils in Zhejiang Province, China

Ning Cui, Xiong Pan & Jing Liu
Dichlorodiphenyltrichloroethane (DDT) is well known for its harmful effects and has been officially banned as a pesticide around the world. However, DDT pollution still exists in natural environments in China because DDT degrade very slowly. In this study, 60 soil samples were collected from Cixi, Zhejiang Province, and 1) DDT and its metabolites were determined, 2) Health risk was estimated by using various indices. The results showed that the detection rate of DDT in soil...

Isolation of Diaphorobacter sp. LW2 capable of degrading Phenanthrene and its migration mediated by Pythium ultimum

Jialu Li, Mei Hong, Rui Tang, Tingchen Cui, Yadong Yang, Jing Lv, Na Liu & Yutao Lei
Phenanthrene, one of polycyclic aromatic hydrocarbons, is stubborn and persistent which exists widely in petroleum contaminated soil. Filamentous fungi are good assistants to bacterial transport, by hyphae passing through soil pores and reaching further positions. An isolated bacterial strain, from the contaminated soil of coking plant, was identified as Diaphorobacter and named LW2, which could use phenanthrene as the only carbon source and energy for its growth. LW2 could degrade phenanthrene in a wide range...

Identification of mite-specific eosinophils in the colon of patients with ulcerative colitis

Shu-Wang Peng, Jiang-Ming Sheng, Bai-Sui Feng, Ke-Ping Peng, Gui-Xiang Tian, Cheng-Bai Liang, Ming-Hui Liu, Hai-Qing Xie, Qing Shu, Yan Li & Ping-Chang Yang
The pathogenesis of ulcerative colitis (UC) is unclear. House dust mite (HDM) is associated with immune inflammation in the body. This study is designed to identify the association between HDM and UC clinical symptoms. UC patients (n = 86) and non-UC control (NC) subjects (n = 64) were recruited. Colon lavage fluids (CLF) were collected from HDM skin prick test positive patients during colonoscopy, and analyzed by immunological approaches. HDM was detected in fecal samples,...

Cloning, homology modeling, heterologous expression and bioinformatic analysis of Ure2pA glutathione S-transferase gene from white rot fungus Trametes gibbosa

Jian Zhang, Yujie Chi, Shuxuan Li, Xinzhi Gu & Yi Ye
Glutathione S-transferases (GSTs) are a class of enzymes with detoxification function. GSTs can be used for the degradation of toxic aromatic compounds, such as dyes. In order to promote their application and cost-effective dye degradation and to understand their structure and function more deeply, we cloned and expressed a gst2 gene from Trametes gibbosa—a white rot fungus. T.gibbosa gst2 has a 672-bp open reading frame (ORF) and encodes 223 amino acids. The recombinant Tg-gst2 in...

Myostain is involved in ginsenoside Rb1-mediated anti-obesity

Hong-Shi Li, Jiang-Ying Kuang, Gui-Jun Liu, Wei-Jie Wu, Xian-Lun Yin, Hao-Dong Li, Lei Wang, Tao Qin, Wen-Cheng Zhang & Yuan-Yuan Sun
Obesity, one of the major public health problems worldwide, has attracted increasing attention. Ginsenoside Rb1 is the most abundant active component of Panax ginseng C.A.Mey (Araliaceae) and is reported to have beneficial effects on obesity and diabetes. However, the mechanisms by which Rb1 regulates obesity remain to be explored. This paper intends to further explore the mechanism of Rb1 in regulating obesity. The C57BL/6 obese mice were divided into two groups: the control (CTR) and...

Total RNA sequencing of Phlebotomus chinensis, a neglected vector in China, simultaneously revealed viral, bacterial, and eukaryotic microbes that are potentially pathogenic to humans

Jing Wang, Qin-yu Gou, Geng-yan Luo, Xin Hou, Guodong Liang & Mang Shi
Phlebotomus chinensis sandfly is a neglected insect vector in China which is well-known for carrying Leishmania. Recent studies have expanded its pathogen repertoire with two novel arthropod-borne phleboviruses capable of infecting human and animals. Despite these discoveries, our knowledge on the general pathogen diversity and overall microbiome composition of this vector species are still very limited. Here we carried out a meta-transcriptomics analysis which simultaneously revealed the actively replicating/transcribing RNA viruses, DNA viruses, bacteria and...

Integrated analysis of long non-coding RNAs and mRNA expression profiles identified potential interactions regulating melanogenesis in chicken skin

Shigang Yu, Gang Wang, Juan Liao, Xuemei Shen & Jia Chen
1. Long non-coding RNAs (lncRNAs) play important roles in various physiological functions. However, the mechanisms underlying the regulation of lncRNAs in melanogenesis remain unclear. To determine the molecular mechanisms involved in the melanogenesis of skin melanogenesis, the present study depicted the expression profiles of lncRNAs and messenger RNAs (mRNAs) in black- (B group) and white- (W group) skinned chickens using RNA sequencing. 2. In total, 373 differentially expressed lncRNAs (DELs; 203 up-regulated and 170 down-regulated)...

Identification of key candidate genes for wing length-related traits by whole-genome resequencing in 772 geese

Jing Ouyang, Yongfei Wu, Yaxi Li, Junjie Miao, Sumei Zheng, Hongbo Tang, Cong Wang, Yanpeng Xiong, Yuren Gao, Luping Wang, Xueming Yan & Hao Chen
1. A total of 772, 420-day-old Xingguo gray geese (XGG) were sequenced using a low-depth (~1×) whole-genome resequencing strategy to reveal the genetic mechanism of wing length-related traits by genome-wide association analysis (GWAS). 2. The results showed that 119 SNPs had genome-wide significance for wing length in five regions of chromosome 4, of which the most significant locus (P=7.95E-11) was located upstream of RBM47 and explained 7.3% of phenotypic variation. 3. A total of 219...

MERS-CoV nsp1 regulates autophagic flux via mTOR signaling and dysfunctional lysosomes

Yujie Feng, Zhaoyi Pan, Zhihui Wang, Zhengyang Lei, Songge Yang, Huajun Zhao, Xueyao Wang, Yating Yu, Qiuju Han & Jian Zhang
Autophagy, a cellular surveillance mechanism, plays an important role in combating invading pathogens. However, viruses have evolved various strategies to disrupt autophagy and even hijack it for replication and release. Here, we demonstrated that Middle East respiratory syndrome coronavirus (MERS-CoV) non-structural protein 1(nsp1) induces autophagy but inhibits autophagic activity. MERS-CoV nsp1 expression increased ROS and reduced ATP levels in cells, which activated AMPK and inhibited the mTOR signaling pathway, resulting in autophagy induction. Meanwhile, as...

Impact of vaccination on kinetics of neutralizing antibodies against SARS-CoV-2 by Serum live neutralization test based on a prospective cohort

Liguo Zhu, Naiying Mao, Changhua Yi, Aidibai Simayi, Jialu Feng, Yi Feng, Min He, Songning Ding, Yin Wang, Yan Wang, Mingwei Wei, Jie Hong, Chuchu Li, Hua Tian, Lu Zhou, Jiefu Peng, Shihan Zhang, Ci Song, Hui Jin, Fengcai Zhu, Wenbo Xu, Jun Zhao & Changjun Bao
How much the vaccine contributes to the induction and development of neutralizing antibodies (NAbs) of breakthrough cases relative to those unvaccinated-infected cases is not fully understood. We conducted a prospective cohort study and collected serum samples from 576 individuals who were diagnosed with SARS-CoV-2 Delta strain infection, including 245 breakthrough cases and 331 unvaccinated-infected cases. NAbs were analyzed by live virus microneutralization test and transformation of NAb titer. NAbs titers against SARS-CoV-2 ancestral and Delta...

Preparation and evaluation of a nanoemulsion containing cordycepin and its protective effect on skin

Hucheng Zhang, Lina Deng, Jun Yang, Guowei Yang, Haitao Fan, Yiqi Yin, Shuai Luo, Shuangshi Li, Linying Liu & Ming Yang
The purpose of this work was to analyze and evaluate the functional properties of a new nanoemulsion that contained cordycepin. Droplet size and distribution were measured, and the stability of the nanoemulsion in different storage conditions was evaluated. We also tested skin irritation caused by the nanoemulsion, cordycepin stability in the nanoemulsion, anti-aging effect, and microbial challenge. The nanoemulsion was homogeneous with proper dispersion stability, and cordycepin was stable for eight months within the cosmetic...

miR-181a-5p can inhibit the proliferation and promote the differentiation of chicken primary myoblasts

Pengtao Yuan, Shengxin Fan, Bin Zhai, Yuanfang Li, Shuaihao Li, Hongtai Li, Hongyuan Zhang, Yanhua Zhang, Ruili Han, Yadong Tian, Guoxi Li & Xiangtao Kang
1. Myoblast proliferation and differentiation is one of the most important biological processes in the development of skeletal muscle. MicroRNAs (miRNAs) play a crucial role in this process. 2. In this study, the expression level of miR-181a-5p was detected, which found that miR-181a-5p was expressed differently in different tissues, different embryonic ages, and different differentiation stages of primary myoblasts in Gushi chickens. 3. The effect of miR-181a-5p was further investigated on chicken primary myoblasts (CPMs)....

Human parainfluenza 3 and respiratory syncytial viruses detected in pangolins

Tengcheng Que, Jing Li, Yugan He, Panyu Chen, Wei Lin, Meihong He, Lei Yu, Aiqiong Wu, Luohao Tan, Yingjiao Li, Yanling Hu & Yigang Tong
Pangolins have gained increasing global attention owing to their public health significance as potential zoonotic hosts since the identification of SARS-CoV-2-related viruses in them. Moreover, these animals could carry other respiratory viruses. In this study, we investigated the virome composition of 16 pangolins that died in 2018 with symptoms of pneumonia using metagenomic approaches. A total of eight whole virus sequences belonging to the Paramyxoviridae or Pneumoviridae families were identified, including one human parainfluenza virus...

Clinical features of the first critical case of acute encephalitis caused by avian influenza A (H5N6) virus

Libing Zhang, Kaituo Liu, Qin Su, Xiao Chen, Xiaoquan Wang, Qingfeng Li, Wenlei Wang, Xuhua Mao, Jinmei Xu, Xin Zhou, Qin Xu, Le Zhou, Xiufan Liu & Pinghu Zhang
Highly pathogenic avian influenza virus (HPAIV) such as H5N1, H5N6, and H7N9 have been reported to frequently infect human, but acute encephalitis caused by HPAIV in human have been rarely reported. We report the first critical case of acute encephalitis with mild pneumonia caused by H5N6 virus. On January 25 of 2022, a 6-year-old girl with severe neurological symptoms was admitted to our hospital and rapidly developed to seizures and coma. Brain imaging showed abnormalities....

Structural insights into broadly neutralizing antibodies against SARS-CoV-2 elicited by hybrid immunity

Mengxiao Luo, Biao Zhou, Eswar R. Reddem, Bingjie Tang, Bohao Chen, Runhong Zhou, Hang Liu, Lihong Liu, Phinikoula S. Katsamba, Ka-Kit Au, Hiu-On Man, Kelvin Kai-Wang To, Kwok-Yung Yuen, Lawrence Shapiro, Shangyu Dang, David D Ho & Zhiwei Chen
Increasing spread by SARS-CoV-2 Omicron variants challenges existing vaccines and broadly reactive neutralizing antibodies (bNAbs) against COVID-19. Here we determine the diversity, potency, breadth and structural insights of bNAbs derived from memory B cells of BNT162b2-vaccinee after homogeneous Omicron BA.1 breakthrough infection. The infection activates diverse memory B cell clonotypes for generating potent class I/II or III bNAbs with new epitopes mapped to receptor-binding domain (RBD). The top eight bNAbs neutralize wildtype and BA.1 potently...

Booster vaccination is required to elicit and maintain COVID-19 vaccine-induced immunity in SIV-infected macaques

Pingchao Li, Qian Wang, Yizi He, Chenchen Yang, Zhengyuan Zhang, Zijian Liu, Bo Liu, Li Yin, Yilan Cui, Peiyu Hu, Yichu Liu, Pingqian Zheng, Wei Wang, Linbing Qu, Caijun Sun, Suhua Guan, Liqiang Feng & Ling Chen
Prolonged infection and possible evolution of SARS-CoV-2 in patients living with uncontrolled HIV-1 infection highlight the importance of an effective vaccination regimen, yet the immunogenicity of COVID-19 vaccines and predictive immune biomarkers have not been well investigated. Herein, we report that the magnitude and persistence of antibody and cell-mediated immunity (CMI) elicited by an Ad5-vectored COVID-19 vaccine are impaired in SIV-infected macaques with high viral loads (> 105 genome copies per ml plasma, SIVhi) but...

Antibody persistence and safety after heterologous boosting with orally aerosolised Ad5-nCoV in individuals primed with two-dose CoronaVac previously: twelve-month analyses of a randomized controlled trial

Lairun Jin, Rong Tang, Shipo Wu, Xiling Guo, Haitao Huang, Lihua Hou, Xiaoqin Chen, Tao Zhu, Jinbo Gou, Jin Zhong, Hongxing Pan, Lunbiao Cui, Yin Chen, Xin Xia, Jialu Feng, Xue Wang, Qi Zhao, XiaoYu Xu, Zhuopei Li, Xiaoyin Zhang, Wei Chen, Jingxin Li & Fengcai Zhu
Antibody persistence and safety up to 12 months of heterologous orally administered adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) in individuals who were primed with two-dose inactivated SARS-CoV-2 vaccine (CoronaVac) previously, has not been reported yet. This randomized, open label, single-center trial included Chinese adults who have received two-dose CoronaVac randomized to low-dose or high-dose aerosolised Ad5-nCoV group, or CoronaVac group. In this report, we mainly evaluated the geometric mean titres (GMTs) of neutralising antibodies (NAbs)...

Preparation and evaluation of a nanoemulsion containing cordycepin and its protective effect on skin

Hucheng Zhang, Lina Deng, Jun Yang, Guowei Yang, Haitao Fan, Yiqi Yin, Shuai Luo, Shuangshi Li, Linying Liu & Ming Yang
The purpose of this work was to analyze and evaluate the functional properties of a new nanoemulsion that contained cordycepin. Droplet size and distribution were measured, and the stability of the nanoemulsion in different storage conditions was evaluated. We also tested skin irritation caused by the nanoemulsion, cordycepin stability in the nanoemulsion, anti-aging effect, and microbial challenge. The nanoemulsion was homogeneous with proper dispersion stability, and cordycepin was stable for eight months within the cosmetic...

Heterologous Omicron-adapted vaccine as a secondary booster promotes neutralizing antibodies against Omicron and its sub-lineages in mice

Jianyang Liu, Qian He, Fan Gao, Lianlian Bian, Qian Wang, Chaoqiang An, Lifang Song, Jialu Zhang, Dong Liu, Ziyang Song, Lu Li, Yu Bai, Zhongfang Wang, Zhenglun Liang, Qunying Mao & Miao Xu
Over one billion people have received 2–3 dosages of an inactivated COVID-19 vaccine for basic immunization. Whether a booster dose should be delivered to protect against the Omicron variant and its sub-lineages, remains controversial. Here, we tested different vaccine platforms targeting the ancestral or Omicron strain as a secondary booster of the ancestral inactivated vaccine in mice. We found that the Omicron-adapted inactivated viral vaccine promoted a neutralizing antibody response against Omicron in mice. Furthermore,...

Registration Year

  • 2022

Resource Types

  • Other


  • Sun Yat-sen University
  • Huazhong University of Science and Technology
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • University of Chinese Academy of Sciences
  • Sichuan University
  • Shanghai Jiao Tong University
  • Capital Medical University
  • Zhejiang University
  • Fudan University
  • Wenzhou Medical University