35 Works

Molecular epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae in China

Xuemei Yang, Qiaoling Sun, Jiaping Li, Yu Jiang, Yi Li, Jianping Lin, Kaichao Chen, Edward Wai-Chi Chan, Rong Zhang & Sheng Chen
The epidemiological features of the newly emerged carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and its potential threat to human health are currently unknown. In this study, a total of 784 blaKPC-2-bearing CRKP strains collected from three hospitals located at different geographical locales in China during 2014-2017 were subjected to molecular typing, screening of virulence plasmid, string test and WGS (367/784 strains). The proportion of CRKP among all clinical K. pneumoniae strains increased sharply in China during...

Comparable antigen-specific T cell responses in vaccinees with diverse humoral immune responses after primary and booster BBIBP-CorV vaccination

Dong Wei, Yingying Chen, Xiaoqi Yu, Yang-dian Lai, Wenxin Xu, Ping Ji, Zhitao Yang, Erzhen Chen, Xinxin Zhang & Ying Wang
BBIBP-CorV exerts efficient protection against SARS-CoV-2 infection. However, waning vaccine-induced humoral immune responses after two-dose vaccination has significantly undermined durable immuno-protection. In this study, we have demonstrated that although anti-spike (S) antibody responses in BBIBP-CorV vaccinees exhibited three serotypes after 6 months including de novo sero-negative, sero-positive and sero-decay features, S-specific interferon-γ release as well as Th1 cytokine production in CD4+ and CD8+ T cells were comparable, especially in vaccinees without detectable neutralizing antibodies. Notably,...

CircSUCO promotes proliferation and differentiation of chicken skeletal muscle satellite cells via sponging miR-15

Yongtong Tian, Xiaoxu Shen, Jing Zhao, Yuanhang Wei, Shunshun Han & Huadong Yin
1. In a previous high-throughput sequencing study, a novel circular RNA (circRNA) generated from a SUN domain containing ossification factor (SUCO) gene transcript (circSUCO) was differentially expressed during the embryonic muscle development. This study aimed to further explore the effect of circSUCO on chicken skeletal muscle development. 2. The experiment analysed the expression patterns of circSUCO in Tianfu broilers and clarified its function in the chicken skeletal muscle satellite cells (SMSC) after circSUCO knockdown. The...

Molecular detection and identification of relapsing fever Borrelia in ticks and wild small mammals in China

Xiao-Ai Zhang, Feng Tian, Yue Li, Xiao-Long Zhang, Bao-Gui Jiang, Bao-Cheng Liu, Jing-Tao Zhang, Shen Tian, Heng Ding, Shuang Li, Hao Li, Li-Qun Fang & Wei Liu
We identified relapsing fever (RF) Borrelia in 1.45% (145/10426) of the ticks and 1.40% (40/2850) of the wild mammals in a field investigation in China. Three RF Borrelia species, including human-pathogenic Borrelia miyamotoi, Borrelia persica and unclassified Babesia sp. were determined. Main species determined from ticks was B. miyamotoi (44.14%), followed by the unclassified Borrelia sp. (42.76%), and Borrelia theileri (13.10%). In wild mammals, main species found was B. persica (57.50%), followed by the unclassified...

Transcriptional Regulation of the Chicken ASMT Gene- A Preliminary Analysis

Jun Teng, Dan Wang, Wenwen Wang, Chao Ning, Qin Zhang & Hui Tang
1. Melatonin is an indole hormone that, among its myriad biological functions, regulates circadian and seasonal rhythms in animals. The ASMT gene plays an essential role in melatonin synthesis. However, in chickens, little is known about the regulatory elements governing its transcription. 2. The following study identified the transcription start site of the chicken ASMT gene by 5’-RACE. Then, the proximal minimal promoter was identified using a series of 5′ truncations of the ASMT promoter...

Epidemiology, evolution, and biological characteristics of H6 avian influenza viruses in China

Xiaohao Xu, Qi Chen, Min Tan, Jia Liu, Xiyan Li, Lei Yang, Yuelong Shu, Dayan Wang & Wenfei Zhu
H6 avian influenza virus (AIV) is one of the most prevalent AIV subtypes in birds globally. To investigate the current situation and characteristics of H6 AIVs circulating in China, we analysed the epidemiology, genetic evolution and pathogenic features of this subtype. During 2000-2021, H6 subtype AIVs spread widely through Southern China and presented high host diversity. On analysing 171 H6 viruses isolated during 2009-2021, dynamic reassortments were observed among H6 and other co-circulating AIV subtypes,...

The prognostic value of ADAMTS8 and its role as a tumor suppressor in breast cancer

Qia Zhang, Quist Kanyomse, Chenghao Luo, Qingfan Mo, XunPing Zhao, Long Wang, Weiyan Peng & Guosheng Ren
A disintegrin-like and metalloprotease with therombospondin type1 motif 8 (ADAMTS8) plays an important role in many malignancies. However, the clinical and biological significance of ADAMTS8 in breast cancer remain unknown. In this study, the clinical data from 1066 breast cancer patients were analyzed by The Cancer Genome Atlas (TCGA) database, and were analyzed using the correlation between ADAMTS8 expression and the clinicopathological features and prognoses. The CCK-8 assay, clone formation assay, flow cytometry and Transwell...

Gendered Injustice: The policing and criminalisation of victim-survivors of domestic and family violence

Emma Russell, Hui Zhou & Gabriela Franich
No description supplied

FBXO34 promotes latent HIV-1 activation by post-transcriptional modulation

Xinyi Yang, Xiaying Zhao, Yuqi Zhu, Jingna Xun, Qin Wen, Hanyu Pan, Jinlong Yang, Jing Wang, Zhimin Liang, Xiaoting Shen, Yue Liang, Qinru Lin, Huitong Liang, Min Li, Jun Chen, Shibo Jiang, Jianqing Xu, Hongzhou Lu & Huanzhang Zhu
Acquired immunodeficiency syndrome (AIDS) cannot be completely cured, mainly due to the existence of a latent HIV-1 reservoir. However, our current understanding of the molecular mechanisms underlying the establishment and maintenance of HIV-1 latent reservoir is not comprehensive. Here, using a genome-wide CRISPR-Cas9 activation library screening, we identified E3 ubiquitin ligase F-box protein 34 (FBXO34) and the substrate of FBXO34, heterogeneous nuclear ribonucleoprotein U (hnRNP U) was identified by affinity purification mass spectrometry, as new...

Isolation of Diaphorobacter sp. LW2 capable of degrading Phenanthrene and its migration mediated by Pythium ultimum

Jialu Li, Mei Hong, Rui Tang, Tingchen Cui, Yadong Yang, Jing Lv, Na Liu & Yutao Lei
Phenanthrene, one of polycyclic aromatic hydrocarbons, is stubborn and persistent which exists widely in petroleum contaminated soil. Filamentous fungi are good assistants to bacterial transport, by hyphae passing through soil pores and reaching further positions. An isolated bacterial strain, from the contaminated soil of coking plant, was identified as Diaphorobacter and named LW2, which could use phenanthrene as the only carbon source and energy for its growth. LW2 could degrade phenanthrene in a wide range...

Transcriptomic analysis of the innate immune signatures of SARS-CoV-2 protein subunit vaccine ZF2001 and a mRNA vaccine RRV

Qian Wang, Ziyang Song, Jinghuan Yang, Qian He, Qunying Mao, Yu Bai, Jianyang Liu, Chaoqiang An, Xujia Yan, Bopei Cui, Lifang Song, Dong Liu, Miao Xu & Zhenglun Liang
Analysis of large-scale gene expression post vaccination can provide an overview of immune responses. We used transcriptional approaches to comprehensively analyze the innate immune response signatures elicited by protein subunit (PS) vaccine ZF2001 and a mRNA vaccine named RRV. A fine-grained time-dependent dissection of large-scale gene expression post immunization revealed that ZF001 induced MHC class II-related genes, including cd74 and H2-Aa, more expeditiously than RRV. Notably, RRV induced MHC class I-related genes like Tap1/2, B2m...

Neutralization of Omicron sublineages and Deltacron SARS-CoV-2 by 3 doses of BNT162b2 vaccine or BA.1 infection

Chaitanya Kurhade, Jing Zou, Hongjie Xia, Mingru Liu, Qi Yang, Mark Cutler, David Cooper, Alexander Muik, Ugur Sahin, Kathrin U. Jansen, Ping Ren, Xuping Xie, Kena A. Swanson & Pei–Yong Shi
Distinct SARS-CoV-2 Omicron sublineages have evolved showing increased fitness and immune evasion than the original Omicron variant BA.1. Here we report the neutralization activity of sera from BNT162b2 vaccinated individuals or unimmunized Omicron BA.1-infected individuals against Omicron sublineages and “Deltacron” variant (XD). BNT162b2 post-dose 3 immune sera neutralized USA-WA1/2020, Omicron BA.1-, BA.2-, BA.2.12.1-, BA.3-, BA.4/5-, and XD-spike SARS-CoV-2s with geometric mean titers (GMTs) of 1335, 393, 298, 315, 216, 103, and 301, respectively; thus, BA.4/5...

mRNA based vaccines provide broad protection against different SARS-CoV-2 variants of concern

Haomeng Wang, Zhao Chen, Zhenghua Wang, Jin Li, Zhihong Yan, Jinbo Yuan, Airu Zhu, Lan Chen, Ye Liu, Chenlong Hu, Ali Zhu, Guowei Li, Yuehu Li, Jie Deng, Liqiao Ma, Xiuwen Sui, Wei Miao, Junqiang Li, Xiuyu Zheng, Jinhua Piao, Yanfeng Yao, Juhong Rao, Chao Shan, Zhiming Yuan, Jincun Zhao … & Tao Zhu
In order to overcome the pandemic of COVID-19, messenger RNA (mRNA)-based vaccine has been extensively researched as a rapid and versatile strategy. Herein, we described the immunogenicity of mRNA-based vaccines for Beta and the most recent Omicron variants. The homologous mRNA-Beta and mRNA-Omicron and heterologous Ad5-nCoV plus mRNA vaccine exhibited high level cross-reactive neutralization for Beta, original, Delta and Omicron variants. It indicated that the COVID-19 mRNA vaccines have great potential in the clinical use...

Apathy in amyotrophic lateral sclerosis: systematic review and meta-analysis of frequency, correlates, and outcomes

Mansur A. Kutlubaev, Jashelle Caga, Ying Xu, Daria K. Areprintseva, Ekaterina V. Pervushina & Matthew C. Kiernan
Objectives: To determine the frequency and correlates of apathy in amyotrophic lateral sclerosis (ALS) and its influence on the prognosis of the disease. Methods: Three databases were searched: MEDLINE, PubMed, and Google Scholar. Quantitative synthesis of the frequency of apathy in ALS was conducted using random effects in Stata (College Station, TX). Meta-regression and subgroup analyses were conducted to investigate the association between frequency of apathy in ALS and different covariates. Results: Fifty-two studies (51...

Humoral and Cellular Immunogenicity and Efficacy of a Coxsackievirus A10 Vaccine in Mice

Huan-Huan An, Meng Li, Rui-Lun Liu, Jie Wu, Sheng-Li Meng, Jing Guo, Ze-Jun Wang, Sha-Sha Qian & Shuo Shen
Coxsackievirus A10 (CV-A10) has become one of the major pathogens of hand, foot and mouth disease (HFMD), and studies on the vaccine and animal model of CV-A10 are still far from complete. Our study used a mouse adapted CV-A10 strain, which was lethal for 14-day-old mice, to develop an infected mouse model. Then this model was employed to establish an actively immunized-challenged mouse model to evaluate the efficacy of a formaldehyde-inactivated CV-A10 vaccine, which was...

Physico-chemical assessment of on-farm bioconversion of organic waste in dairy farms in context to sustainability and circular bioeconomy

Pramod K. Pandey, Brahmaver D. Shetty, Patrick Wickam, Peiman Aminabadi, Zhao Chen, Kelly Mai, Jeffery W. Stackhouse & Michele T. Jay-Russell
On a milk-producing dairy farm, milk production is correlated with manure production and number of cattle, and manure is widely used as soil fertilizer. However, excessive dairy manure production is linked with greenhouse gas emissions and water quality issues. On-farm planning of manure storage and application to enhance soil nutrients are essential in a circular economy to reduce environmental impact, where manure is not landfilled and incinerated. Instead, it creates a nutrient resource for crops,...

Genetic, biological and epidemiological study on a cluster of H9N2 avian influenza virus infections among chickens, a pet cat, and humans at a backyard farm in Guangxi, China

Jing Yang, Jianhua Yan, Cheng Zhang, Shanqin Li, Manhua Yuan, Chunge Zhang, Chenguang Shen, Yang Yang, Lifeng Fu, Guanlong Xu, Weifeng Shi, Zhenghai Ma, Ting Rong Luo & Yuhai Bi
During an investigation in October 2018, two people with diarrhoea, mild abdominal pain, and mild arthralgia symptoms in Guangxi, China, were identified as infected by H9N2 avian influenza virus (AIV). Four H9N2 AIVs were isolated from one of two patients, a pet cat, and a dead chicken (two respective isolates from its lung and kidney tissues) bred by the patients at a backyard farm. Epidemiological investigation indicated that the newly bought chicken died first, and...

Interactions between host epithelial cells and Acinetobacter baumannii promote the emergence of highly antibiotic resistant and highly mucoid strains

Wang Zhang, Yue Yao, Hua Zhou, Jintao He, Jingfen Wang, Li Li, Minsong Gao, Xiaochen Liu, Ya Shi, Jinzhong Lin, Jianzhao Liu, Huan Chen, Yu Feng, Zhihui Zhou, Yunsong Yu & Xiaoting Hua
Acinetobacter baumannii is an important nosocomial pathogen. Upon colonizing a host, A. baumannii are subjected to selective pressure by immune defenses as they adapt to the host environment. However, the mechanism of this pathoadaptation is unknown. Here, we established an in vitro system to evolve A. baumannii driven by the continuous selective pressure exerted by epithelial cells, and we used a combination of experimental evolution, phenotypic characterization and multi-omics analysis to address the underlying mechanism....

Booster vaccination is required to elicit and maintain COVID-19 vaccine-induced immunity in SIV-infected macaques

Pingchao Li, Qian Wang, Yizi He, Chenchen Yang, Zhengyuan Zhang, Zijian Liu, Bo Liu, Li Yin, Yilan Cui, Peiyu Hu, Yichu Liu, Pingqian Zheng, Wei Wang, Linbing Qu, Caijun Sun, Suhua Guan, Liqiang Feng & Ling Chen
Prolonged infection and possible evolution of SARS-CoV-2 in patients living with uncontrolled HIV-1 infection highlight the importance of an effective vaccination regimen, yet the immunogenicity of COVID-19 vaccines and predictive immune biomarkers have not been well investigated. Herein, we report that the magnitude and persistence of antibody and cell-mediated immunity (CMI) elicited by an Ad5-vectored COVID-19 vaccine are impaired in SIV-infected macaques with high viral loads (> 105 genome copies per ml plasma, SIVhi) but...

Antibody persistence and safety after heterologous boosting with orally aerosolised Ad5-nCoV in individuals primed with two-dose CoronaVac previously: twelve-month analyses of a randomized controlled trial

Lairun Jin, Rong Tang, Shipo Wu, Xiling Guo, Haitao Huang, Lihua Hou, Xiaoqin Chen, Tao Zhu, Jinbo Gou, Jin Zhong, Hongxing Pan, Lunbiao Cui, Yin Chen, Xin Xia, Jialu Feng, Xue Wang, Qi Zhao, XiaoYu Xu, Zhuopei Li, Xiaoyin Zhang, Wei Chen, Jingxin Li & Fengcai Zhu
Antibody persistence and safety up to 12 months of heterologous orally administered adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) in individuals who were primed with two-dose inactivated SARS-CoV-2 vaccine (CoronaVac) previously, has not been reported yet. This randomized, open label, single-center trial included Chinese adults who have received two-dose CoronaVac randomized to low-dose or high-dose aerosolised Ad5-nCoV group, or CoronaVac group. In this report, we mainly evaluated the geometric mean titres (GMTs) of neutralising antibodies (NAbs)...

Heterologous Omicron-adapted vaccine as a secondary booster promotes neutralizing antibodies against Omicron and its sub-lineages in mice

Jianyang Liu, Qian He, Fan Gao, Lianlian Bian, Qian Wang, Chaoqiang An, Lifang Song, Jialu Zhang, Dong Liu, Ziyang Song, Lu Li, Yu Bai, Zhongfang Wang, Zhenglun Liang, Qunying Mao & Miao Xu
Over one billion people have received 2–3 dosages of an inactivated COVID-19 vaccine for basic immunization. Whether a booster dose should be delivered to protect against the Omicron variant and its sub-lineages, remains controversial. Here, we tested different vaccine platforms targeting the ancestral or Omicron strain as a secondary booster of the ancestral inactivated vaccine in mice. We found that the Omicron-adapted inactivated viral vaccine promoted a neutralizing antibody response against Omicron in mice. Furthermore,...

Succession of the microbial communities and metabolic functions in composting or deep burial processing of dead chickens

Zhongzhen Lin, Chaowu Yang, Bo Xia, Mohan Qiu, Han Peng, Xiaosong Jiang, Huarui Du, Qingyun Li, Zengrong Zhang, Yiping Liu & Chunlin Yu
1. This study examined the effects of composting and deep burial techniques on degradation efficiency of dead chickens. Different raw materials (crushed branches or rape straws) and disinfectants (quicklime or bleaching powder) were applied in composting and deep burial process, respectively. The whole process lasted for 90 d in both summer and winter. 2. High throughput sequencing displayed that Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria and Deinococcus-Thermus were the most dominant bacterial phyla during the experiment. The...

Adjunctive Zoledronate+IL-2 administrations enhance anti-tuberculosis Vγ2Vδ2 T-effector populations, and improve treatment outcome of multidrug-resistant tuberculosis.

Hongbo Shen, Enzhuo Yang, Ming Guo, Rui Yang, Guixian Huang, Ying Peng, Wei Sha, Feifei Wang & Ling Shen
Multidrug-resistant tuberculosis(MDR-TB) is a refractory disease with high mortality rate due to no or few choices of antibiotics. Adjunctive immunotherapy may help improve treatment outcome of MDR-TB. Our decade-long studies demonstrated that phosphoantigen-specific Vγ2Vδ2 T cells play protective roles in immunity against TB. Here, we hypothesized that enhancing protective Vγ2Vδ2 T-effector cells could improve treatment outcome of MDR-TB. To address this, we employed clinically-approved drugs Zoledronate(ZOL) and IL-2 to induce anti-TB Vγ2Vδ2 T-effector cells as...

Transcriptome profiling of morphogenetic differences between contour and flight feathers in duck

Qian Xu, Yang Xi, Shengchao Ma, Jianmei Wang, Junpeng Li, Chunchun Han, Liang Li, Jiwen Wang & Hehe Liu
1. This study examined the transcriptomic profiles of contour and flight feather follicles from two duck breeds to determine the molecular network and the candidate genes associated with contour and flight feather morphogenesis. 2. High-throughput RNA sequencing was performed to compare differences in feather follicles between contour and flight feathers in two duck breeds (Heiwu and Nonghua duck). 3. Comparing the contour feather follicles with flight feather follicles, 4,757 and 4,820 differentially expressed genes (DEGs)...

Total RNA sequencing of Phlebotomus chinensis, a neglected vector in China, simultaneously revealed viral, bacterial, and eukaryotic microbes that are potentially pathogenic to humans

Jing Wang, Qin-yu Gou, Geng-yan Luo, Xin Hou, Guodong Liang & Mang Shi
Phlebotomus chinensis sandfly is a neglected insect vector in China which is well-known for carrying Leishmania. Recent studies have expanded its pathogen repertoire with two novel arthropod-borne phleboviruses capable of infecting human and animals. Despite these discoveries, our knowledge on the general pathogen diversity and overall microbiome composition of this vector species are still very limited. Here we carried out a meta-transcriptomics analysis which simultaneously revealed the actively replicating/transcribing RNA viruses, DNA viruses, bacteria and...

Registration Year

  • 2022

Resource Types

  • Other


  • Central South University
  • Zhejiang University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • University of Chinese Academy of Sciences
  • Huazhong University of Science and Technology
  • Sichuan University
  • Sun Yat-sen University
  • West China Hospital of Sichuan University
  • Air Force Medical University