Background: Critically ill patients with COVID-19 are of grave concern. Those patients usually underwent a stage of excessive inflammation before developing acute respiratory distress syndrome. In this study, we test the hypothesis that short-term, low-to-moderate-dose corticosteroids would benefit patients when used in the early phase of excessive inflammation namely, the therapeutic window. Methods: Among a Shanghai cohort and a validation cohort, we enrolled COVID-19 patients showing marked radiographic progression. Short-term, low-to-moderate-dose corticosteroids were considered for...

Supplementary Table 1 Positive rate of different MODY screening criteria Supplementary Table 2 The clinical and genetic characteristics of MODY in two cohorts (gray lines represent the cohort 2)

Changes in the activated sludge performance in an anaerobic/aerobic biological treatment system for leachate was discussed under the condition of tetracycline (TC) exposure. The results show that a low concentration of TC did not have an obvious effect on the removal of chemical oxygen demand (COD) while a high concentration of TC had a certain promoting effect. Under the stimulation of TC, the particle size distribution of anaerobic/aerobic sludge tended to be more uniform, the...

Combinatorial chemo-photodynamic therapy is regared as effective cancer therapy strategy, which could be realized via multiple nano-drug delivery system. Herein, novel high payload nanoparticles stabilized by amphiphilic block polymer cholesterol-b-poly(ethylene glycol) (PEG)2000 (Chol-PEG2000) were fabricated for loading chemotherapeutic drug 10-hydroxycamptothecin (HCPT) and photosensitizer chlorin e6 (Ce6). The obtained HCPT/Ce6 NPs showed uniform rod-like morphology with a hydration diameter of 178.9 ± 4.0 nm and excellent stability in aqueous solution. HCPT and Ce6 in the NPs...

The fitness of multidrug-resistant tuberculosis (MDR-TB) is thought to be an important determinant of a strain’s ability to be transmitted and cause outbreaks. Studies in the laboratory have demonstrated that MDR-TB strains have reduced fitness but the relative transmissibility of MDR-TB versus drug-susceptible (DS) TB strains in human populations remains unresolved. We used data on genomic clustering from our previous molecular epidemiological study in Songjiang (2011-2020) and Wusheng (2009-2020), China, to compare the relative transmissibility...

A new C19-diterpenoid alkaloid named gyalanutine A (1) and fourteen known compounds 2-15 were isolated from the plant of Delphinium gyalanum C. Marquand & Airy Shaw. Compound 1 displayed an unusual lycoctonine-type C19-diterpenoid alkaloid skeleton with the cleavage of N-C19 and C7-C17 bonds, and the construction of the N-C7 bond. Structures were identified by multiple spectroscopic analyses including 1 D, 2 D NMR, IR and HR-ESI-MS. Compounds were tested for acetylcholinesterase inhibitory and anti-inflammatory activity.

Supplementary Table 1 Positive rate of different MODY screening criteria Supplementary Table 2 The clinical and genetic characteristics of MODY in two cohorts (gray lines represent the cohort 2)

A disintegrin-like and metalloprotease with therombospondin type1 motif 8 (ADAMTS8) plays an important role in many malignancies. However, the clinical and biological significance of ADAMTS8 in breast cancer remain unknown. In this study, the clinical data from 1066 breast cancer patients were analyzed by The Cancer Genome Atlas (TCGA) database, and were analyzed using the correlation between ADAMTS8 expression and the clinicopathological features and prognoses. The CCK-8 assay, clone formation assay, flow cytometry and Transwell...

Nonparametric control charts that can detect arbitrary distributional changes are highly desirable due to their flexibility to adapt to different distributional assumptions and changes. However, most of the nonparametric control charts in the literature either can only detect location changes, or involve intensive computation. In this article, we propose a new nonparametric adaptive CUSUM chart. The proposed control chart can detect arbitrary distributional changes and is computationally efficient. Its self-starting nature makes the proposed control...

The epidemiological features of the newly emerged carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and its potential threat to human health are currently unknown. In this study, a total of 784 blaKPC-2-bearing CRKP strains collected from three hospitals located at different geographical locales in China during 2014-2017 were subjected to molecular typing, screening of virulence plasmid, string test and WGS (367/784 strains). The proportion of CRKP among all clinical K. pneumoniae strains increased sharply in China during...

Bismuth-based photocatalytic technology has been a promising way to degrade contaminants in the aqueous system. In this work, a direct Z-scheme BiOCl/Bi3NbO7 heterojunction with a different molar ratio of Bi and Nb elements was fabricated through a facile in situ growth method. The structural and photoelectric characteristics of these as-prepared samples were investigated by SEM, XPS, TEM, XRD, BET, UV-vis DRS, PL, EIS and TPC. The photocatalytic activity was evaluated by the degradation of RhB,...

Combinatorial chemo-photodynamic therapy is regared as effective cancer therapy strategy, which could be realized via multiple nano-drug delivery system. Herein, novel high payload nanoparticles stabilized by amphiphilic block polymer cholesterol-b-poly(ethylene glycol) (PEG)2000 (Chol-PEG2000) were fabricated for loading chemotherapeutic drug 10-hydroxycamptothecin (HCPT) and photosensitizer chlorin e6 (Ce6). The obtained HCPT/Ce6 NPs showed uniform rod-like morphology with a hydration diameter of 178.9 ± 4.0 nm and excellent stability in aqueous solution. HCPT and Ce6 in the NPs...

Candida parapsilosis is becoming a predominant non-albicans cause of invasive candidiasis (IC). Echinocandins are the preferred choice for IC treatment and prophylaxis. Resistance to echinocandins in C. parapsilosis has emerged in several countries, but little is known about the susceptibility profile in China or about mechanisms of resistance. Here, we investigated the echinocandin susceptibilities of 2523 C. parapsilosis isolates collected from China and further explored the resistance mechanism among echinocandin-resistant isolates. Anidulafungin exhibited the highest...

Rickettsia heilongjiangensis, a tick-borne obligate intracellular bacterium and causative agent of spotted fever in China, has attracted increasing concern regarding its capability in causing human rickettsiosis. Here, we conducted a genomic analysis of a new Rickettsia heilongjiangensis strain B8 (B8) isolated from the serum of a patient who had been bitten by a Haemaphysalis longicornis tick in Anhui Province, China. The present study sought to identify exclusive genes that might be associated with the pathogenicity...

Introduction: Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes. Methods: The postpartum placental tissue of pregnant women with ICP were chosen as the...

Autism spectrum disorder (ASD) is one of the common neurodevelopmental disorders in children. Its etiology and pathogenesis are poorly understood. Previous studies suggest potential changes in the complement and coagulation pathways in individuals with ASD.The etiology and pathogenesis have not been clarified. Studies have shown that complement and coagulation pathway were related to ASD. In this study, using multiple reaction monitoring proteomic technology, 16 of the 33 proteins involved in this pathway were identified as...

Autism spectrum disorder (ASD) is one of the common neurodevelopmental disorders in children. Its etiology and pathogenesis are poorly understood. Previous studies suggest potential changes in the complement and coagulation pathways in individuals with ASD.The etiology and pathogenesis have not been clarified. Studies have shown that complement and coagulation pathway were related to ASD. In this study, using multiple reaction monitoring proteomic technology, 16 of the 33 proteins involved in this pathway were identified as...

In order to effectively inhibit the explosion of titanium powder, this study used the Hartmann tube experimental system and the 20 L explosion tank experimental system to carry out flame propagation and explosion overpressure experiments, respectively, and the effect of NH4H2PO4 on the explosion suppression of titanium powder was studied. The results show that when the addition of NH4H2PO4 powder is 50%, the flame propagation velocity and explosion pressure can be reduced to the minimum,...

In order to effectively inhibit the explosion of titanium powder, this study used the Hartmann tube experimental system and the 20 L explosion tank experimental system to carry out flame propagation and explosion overpressure experiments, respectively, and the effect of NH4H2PO4 on the explosion suppression of titanium powder was studied. The results show that when the addition of NH4H2PO4 powder is 50%, the flame propagation velocity and explosion pressure can be reduced to the minimum,...

The fitness of multidrug-resistant tuberculosis (MDR-TB) is thought to be an important determinant of a strain’s ability to be transmitted and cause outbreaks. Studies in the laboratory have demonstrated that MDR-TB strains have reduced fitness but the relative transmissibility of MDR-TB versus drug-susceptible (DS) TB strains in human populations remains unresolved. We used data on genomic clustering from our previous molecular epidemiological study in Songjiang (2011-2020) and Wusheng (2009-2020), China, to compare the relative transmissibility...

1. Methyltransferase-like 21C (METTL21C) and insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) play important roles in the proliferation of chicken myoblasts. However, it remains unclear whether there is protein-protein interaction between METTL21C and IGF2BP1 to regulate proliferation of chicken myoblasts. 2. In this study, the Igf2bp1 gene was amplified from cDNA of liver tissue of Lueyang black-bone chicken to construct the overexpression vector HA-Igf2bp1. The HA-Igf2bp1 and Flag-Mettl21c vectors were individually transfected and co-transfected...

Nonparametric control charts that can detect arbitrary distributional changes are highly desirable due to their flexibility to adapt to different distributional assumptions and changes. However, most of the nonparametric control charts in the literature either can only detect location changes, or involve intensive computation. In this article, we propose a new nonparametric adaptive CUSUM chart. The proposed control chart can detect arbitrary distributional changes and is computationally efficient. Its self-starting nature makes the proposed control...

Waning of neutralizing titers along with decline of protection efficacy after the second dose of COVID-19 vaccines was observed, including China-made inactivated vaccines. Efficacy of a heterologous boosting using one dose of a recombinant SARS-CoV-2 fusion protein vaccine (V-01) in inactivated vaccine-primed population was studied, aimed to restore the immunity. A randomized, double-blind and placebo-controlled phase Ⅲ trial was conducted in healthy people aged 18 years or older in Pakistan and Malaysia. Each eligible participant...

Introduction: Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes. Methods: The postpartum placental tissue of pregnant women with ICP were chosen as the...

The role of thyroid hormones in tumors has been discussed in observational studies; however, the causal relationship between them remains controversial. Six different Mendelian randomization (MR) Methods, including Inverse variance weighted (IVW), MR-Egger, Simple mode, MR-Pleiotropy Residual Sum and Outlier methods (MR-PRESSO), Weighted mode and Weighted median were used to Two-Sample MR analysis. Our research shows that hypothyroidism genetically has a protective causal association with lung cancer.