22 Works

Healthy Outcomes of Patients with COVID-19 Two Years after the Infection: A Prospective Cohort Study

Dapeng Li, Xuejiao Liao, Zhi Liu, Zhenghua Ma, Jingke Dong, Guoqin Zheng, Mei Zi, Fang Wang, Qing He, Guobao Li, Zheng Zhang & Lei Liu
The long-term effect of coronavirus disease 2019 (COVID-19) has been rarely known. This study aimed to investigate healthy outcomes of COVID-19 survivors up to 2 years after the infection. A total of 155 COVID-19 patients, who were discharged from Shenzhen Third People's Hospital from February 2020 to April 2020, were enrolled and followed up until March 4, 2022. COVID-19 survivors received questionnaires of long COVID symptoms and psychological symptoms, pulmonary function tests, chest computed tomography...

Succession of the microbial communities and metabolic functions in composting or deep burial processing of dead chickens

Zhongzhen Lin, Chaowu Yang, Bo Xia, Mohan Qiu, Han Peng, Xiaosong Jiang, Huarui Du, Qingyun Li, Zengrong Zhang, Yiping Liu & Chunlin Yu
1. This study examined the effects of composting and deep burial techniques on degradation efficiency of dead chickens. Different raw materials (crushed branches or rape straws) and disinfectants (quicklime or bleaching powder) were applied in composting and deep burial process, respectively. The whole process lasted for 90 d in both summer and winter. 2. High throughput sequencing displayed that Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria and Deinococcus-Thermus were the most dominant bacterial phyla during the experiment. The...

Transcriptome profiling of morphogenetic differences between contour and flight feathers in duck

Qian Xu, Yang Xi, Shengchao Ma, Jianmei Wang, Junpeng Li, Chunchun Han, Liang Li, Jiwen Wang & Hehe Liu
1. This study examined the transcriptomic profiles of contour and flight feather follicles from two duck breeds to determine the molecular network and the candidate genes associated with contour and flight feather morphogenesis. 2. High-throughput RNA sequencing was performed to compare differences in feather follicles between contour and flight feathers in two duck breeds (Heiwu and Nonghua duck). 3. Comparing the contour feather follicles with flight feather follicles, 4,757 and 4,820 differentially expressed genes (DEGs)...

Identification of key candidate genes for wing length-related traits by whole-genome resequencing in 772 geese

Jing Ouyang, Yongfei Wu, Yaxi Li, Junjie Miao, Sumei Zheng, Hongbo Tang, Cong Wang, Yanpeng Xiong, Yuren Gao, Luping Wang, Xueming Yan & Hao Chen
1. A total of 772, 420-day-old Xingguo gray geese (XGG) were sequenced using a low-depth (~1×) whole-genome resequencing strategy to reveal the genetic mechanism of wing length-related traits by genome-wide association analysis (GWAS). 2. The results showed that 119 SNPs had genome-wide significance for wing length in five regions of chromosome 4, of which the most significant locus (P=7.95E-11) was located upstream of RBM47 and explained 7.3% of phenotypic variation. 3. A total of 219...

Referral criteria to palliative care for patients with Parkinson’s disease: a systematic review

Yiping Chen, Liyuan Hou, Wei Li, Qiaohong Wang, Wentao Zhou & Hui Yang
Objective: This systematic review aimed to identify the referral criteria for palliative care in patients with Parkinson’s disease. Methods: We conducted an electronic search for publications on referral criteria for palliative care in patients with Parkinson’s disease in six electronic databases. The articles were thoroughly reviewed by two independent reviewers for inclusion using a predefined data extraction list. The referral criteria were thematically classified using a coding methodology. Results: This systematic review included 36 publications....

Total RNA sequencing of Phlebotomus chinensis, a neglected vector in China, simultaneously revealed viral, bacterial, and eukaryotic microbes that are potentially pathogenic to humans

Jing Wang, Qin-yu Gou, Geng-yan Luo, Xin Hou, Guodong Liang & Mang Shi
Phlebotomus chinensis sandfly is a neglected insect vector in China which is well-known for carrying Leishmania. Recent studies have expanded its pathogen repertoire with two novel arthropod-borne phleboviruses capable of infecting human and animals. Despite these discoveries, our knowledge on the general pathogen diversity and overall microbiome composition of this vector species are still very limited. Here we carried out a meta-transcriptomics analysis which simultaneously revealed the actively replicating/transcribing RNA viruses, DNA viruses, bacteria and...

Efficacy of heterologous boosting against SARS-CoV-2 using a recombinant interferon-armed fusion protein vaccine (V-01): a randomized, double-blind and placebo-controlled phase III trial

Xuan-Yi Wang, Syed Faisal Mahmood, Fang Jin, Wee Kooi Cheah, Muhammad Ahmad, Mian Amjad Sohail, Waheed Ahmad, Vijaya K Suppan, Muneeba Ahsan Sayeed, Shobha Luxmi, Aik-Howe Teo, Li Yuan Lee, Yang-Yang Qi, Rong-Juan Pei, Wei Deng, Zhong-Hui Xu, Jia-Ming Yang, Yan Zhang, Wu-Xiang Guan & Xiong Yu
Waning of neutralizing titers along with decline of protection efficacy after the second dose of COVID-19 vaccines was observed, including China-made inactivated vaccines. Efficacy of a heterologous boosting using one dose of a recombinant SARS-CoV-2 fusion protein vaccine (V-01) in inactivated vaccine-primed population was studied, aimed to restore the immunity. A randomized, double-blind and placebo-controlled phase Ⅲ trial was conducted in healthy people aged 18 years or older in Pakistan and Malaysia. Each eligible participant...

RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variant

Jianing Yang, Xuejiao Hu, Wenzhuo Wang, Yujing Yang, Xinqiang Zhang, Wei Fang, Lei Zhang, Shan Li & Bing Gu
The highly infectious Delta variant strain of SARS-CoV-2 remains globally dominant and undermines COVID-19 vaccines. Rapid detection of the Delta variant is crucial for the identification and quarantine of infected individuals. In this study, our aim was to design and validate a genotyping RT-LAMP method to detect Delta variants specifically. R203M in the N gene of SARS-CoV-2 was chosen as the Delta variant-specific mutation for genotyping. To target the R203M-harboring region and the conserved sequence...

Integrated analysis of long non-coding RNAs and mRNA expression profiles identified potential interactions regulating melanogenesis in chicken skin

Shigang Yu, Gang Wang, Juan Liao, Xuemei Shen & Jia Chen
1. Long non-coding RNAs (lncRNAs) play important roles in various physiological functions. However, the mechanisms underlying the regulation of lncRNAs in melanogenesis remain unclear. To determine the molecular mechanisms involved in the melanogenesis of skin melanogenesis, the present study depicted the expression profiles of lncRNAs and messenger RNAs (mRNAs) in black- (B group) and white- (W group) skinned chickens using RNA sequencing. 2. In total, 373 differentially expressed lncRNAs (DELs; 203 up-regulated and 170 down-regulated)...

FBXO34 promotes latent HIV-1 activation by post-transcriptional modulation

Xinyi Yang, Xiaying Zhao, Yuqi Zhu, Jingna Xun, Qin Wen, Hanyu Pan, Jinlong Yang, Jing Wang, Zhimin Liang, Xiaoting Shen, Yue Liang, Qinru Lin, Huitong Liang, Min Li, Jun Chen, Shibo Jiang, Jianqing Xu, Hongzhou Lu & Huanzhang Zhu
Acquired immunodeficiency syndrome (AIDS) cannot be completely cured, mainly due to the existence of a latent HIV-1 reservoir. However, our current understanding of the molecular mechanisms underlying the establishment and maintenance of HIV-1 latent reservoir is not comprehensive. Here, using a genome-wide CRISPR-Cas9 activation library screening, we identified E3 ubiquitin ligase F-box protein 34 (FBXO34) and the substrate of FBXO34, heterogeneous nuclear ribonucleoprotein U (hnRNP U) was identified by affinity purification mass spectrometry, as new...

Molecular detection and identification of relapsing fever Borrelia in ticks and wild small mammals in China

Xiao-Ai Zhang, Feng Tian, Yue Li, Xiao-Long Zhang, Bao-Gui Jiang, Bao-Cheng Liu, Jing-Tao Zhang, Shen Tian, Heng Ding, Shuang Li, Hao Li, Li-Qun Fang & Wei Liu
We identified relapsing fever (RF) Borrelia in 1.45% (145/10426) of the ticks and 1.40% (40/2850) of the wild mammals in a field investigation in China. Three RF Borrelia species, including human-pathogenic Borrelia miyamotoi, Borrelia persica and unclassified Babesia sp. were determined. Main species determined from ticks was B. miyamotoi (44.14%), followed by the unclassified Borrelia sp. (42.76%), and Borrelia theileri (13.10%). In wild mammals, main species found was B. persica (57.50%), followed by the unclassified...

Epidemiology, evolution, and biological characteristics of H6 avian influenza viruses in China

Xiaohao Xu, Qi Chen, Min Tan, Jia Liu, Xiyan Li, Lei Yang, Yuelong Shu, Dayan Wang & Wenfei Zhu
H6 avian influenza virus (AIV) is one of the most prevalent AIV subtypes in birds globally. To investigate the current situation and characteristics of H6 AIVs circulating in China, we analysed the epidemiology, genetic evolution and pathogenic features of this subtype. During 2000-2021, H6 subtype AIVs spread widely through Southern China and presented high host diversity. On analysing 171 H6 viruses isolated during 2009-2021, dynamic reassortments were observed among H6 and other co-circulating AIV subtypes,...

Exposure to di (2-ethylhexyl) phthalate causes locomotor increase and anxiety-like behavior via induction of oxidative stress in brain

Shixin Tang, Hongyang Zhang, Yinyin Xia, Shiyue Luo, Yijun Liu, Xinhao Duan, Zhen Zou, Chengzhi Chen, Lixiao Zhou & Jingfu Qiu
Di (2-ethylhexyl) phthalate (DEHP) is the most prevalent exposure scenario in the daily life. A growing number of significant studies showed that DEHP could exhibit long-term adverse health effects for the human body, particularly in the liver, kidneys, heart and reproductive systems. However, the impact of oral intake of DEHP on nervous system is extremely limited. In the present study, the adult C57BL/6J male mice were intragastric administered with two dosages DEHP for 35 days....

Omicron infections profile and vaccination status among 1881 liver transplant recipients: a multi-center retrospective cohort

Ensi Ma, Jingwen Ai, Yi Zhang, Jianming Zheng, Xiaogang Gao, Junming Xu, Hao Yin, Zhiren Fu, Hao Xing, Li Li, Liying Sun, Heyu Huang, Quanbao Zhang, Linlin Xu, Yanting Jin, Rui Chen, Guoyue Lv, Zhijun Zhu, Wenhong Zhang & Zhengxin Wang
A wave of Omicron infections rapidly emerged in China in 2022, but large-scale data concerning the safety profile of vaccines and Coronavirus disease 2019 (COVID-19) infection features in liver transplant (LT) recipients have not been collected. Therefore, the aim of this study was to assess the protectiveness and safety profile of the inactivated vaccines in LT patients against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant infections. A multi-center retrospective study was conducted in...

Speeding up Genetic Programming Based Symbolic Regression Using GPUs

Rui Zhang, Andrew Lensen & Yanan Sun
No description supplied

Decreased echinocandin susceptibility in Candida parapsilosis causing candidemia and emergence of a pan-echinocandin resistant case in China

Yating Ning, Meng Xiao, David S. Perlin, Yanan Zhao, Minya Lu, Yi Li, Zhengyu Luo, Rongchen Dai, Shengjie Li, Jiajun Xu, Lingli Liu, Hong He, Yun Liu, Fushun Li, Yuguang Guo, Zhongju Chen, Yingchun Xu, Tianshu Sun & Li Zhang
Candida parapsilosis is becoming a predominant non-albicans cause of invasive candidiasis (IC). Echinocandins are the preferred choice for IC treatment and prophylaxis. Resistance to echinocandins in C. parapsilosis has emerged in several countries, but little is known about the susceptibility profile in China or about mechanisms of resistance. Here, we investigated the echinocandin susceptibilities of 2523 C. parapsilosis isolates collected from China and further explored the resistance mechanism among echinocandin-resistant isolates. Anidulafungin exhibited the highest...

Complete genome sequencing and comparative genomic analyses of a new spotted-fever Rickettsia heilongjiangensis strain B8

Maozhang He, Lifeng Zhang, Haoran Hu, Xiaohan Liu, Cong Zhang, Yu Xin, Boyu Liu, Zhen Chen, Kehan Xu & Yan Liu
Rickettsia heilongjiangensis, a tick-borne obligate intracellular bacterium and causative agent of spotted fever in China, has attracted increasing concern regarding its capability in causing human rickettsiosis. Here, we conducted a genomic analysis of a new Rickettsia heilongjiangensis strain B8 (B8) isolated from the serum of a patient who had been bitten by a Haemaphysalis longicornis tick in Anhui Province, China. The present study sought to identify exclusive genes that might be associated with the pathogenicity...

Spectral correlation between wheat genotype replications over the visible and near-infrared spectrum

Xuebin Yang, Xu Wang, Byron Evers, Yaokui Cui & Jesse Poland
The growth stage best suitable for wheat yield phenotyping has been a hot topic. This study provides a fresh insight into it, from the perspective of spectral correlation between wheat genotype replications. A number of 340 wheat genotypes and their replication were distributed in separate parts (west and east) of an experiment field (2019–2020) in Ashland, Kansas, USA. Unmanned aerial vehicle based hyperspectral images (400–100 nm) of the experiment field were taken over the late...

METTL21C mediates lysine trimethylation of IGF2BP1 to regulate chicken myoblast proliferation

Shanshan Wang, Jiarong Zhao, Ling Wang, Tao Zhang, Wenxian Zeng & Hongzhao Lu
1. Methyltransferase-like 21C (METTL21C) and insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) play important roles in the proliferation of chicken myoblasts. However, it remains unclear whether there is protein-protein interaction between METTL21C and IGF2BP1 to regulate proliferation of chicken myoblasts. 2. In this study, the Igf2bp1 gene was amplified from cDNA of liver tissue of Lueyang black-bone chicken to construct the overexpression vector HA-Igf2bp1. The HA-Igf2bp1 and Flag-Mettl21c vectors were individually transfected and co-transfected...

Antibody persistence and safety after heterologous boosting with orally aerosolised Ad5-nCoV in individuals primed with two-dose CoronaVac previously: twelve-month analyses of a randomized controlled trial

Lairun Jin, Rong Tang, Shipo Wu, Xiling Guo, Haitao Huang, Lihua Hou, Xiaoqin Chen, Tao Zhu, Jinbo Gou, Jin Zhong, Hongxing Pan, Lunbiao Cui, Yin Chen, Xin Xia, Jialu Feng, Xue Wang, Qi Zhao, XiaoYu Xu, Zhuopei Li, Xiaoyin Zhang, Wei Chen, Jingxin Li & Fengcai Zhu
Antibody persistence and safety up to 12 months of heterologous orally administered adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) in individuals who were primed with two-dose inactivated SARS-CoV-2 vaccine (CoronaVac) previously, has not been reported yet. This randomized, open label, single-center trial included Chinese adults who have received two-dose CoronaVac randomized to low-dose or high-dose aerosolised Ad5-nCoV group, or CoronaVac group. In this report, we mainly evaluated the geometric mean titres (GMTs) of neutralising antibodies (NAbs)...

Spectral correlation between wheat genotype replications over the visible and near-infrared spectrum

Xuebin Yang, Xu Wang, Byron Evers, Yaokui Cui & Jesse Poland
The growth stage best suitable for wheat yield phenotyping has been a hot topic. This study provides a fresh insight into it, from the perspective of spectral correlation between wheat genotype replications. A number of 340 wheat genotypes and their replication were distributed in separate parts (west and east) of an experiment field (2019–2020) in Ashland, Kansas, USA. Unmanned aerial vehicle based hyperspectral images (400–100 nm) of the experiment field were taken over the late...

Interactions between host epithelial cells and Acinetobacter baumannii promote the emergence of highly antibiotic resistant and highly mucoid strains

Wang Zhang, Yue Yao, Hua Zhou, Jintao He, Jingfen Wang, Li Li, Minsong Gao, Xiaochen Liu, Ya Shi, Jinzhong Lin, Jianzhao Liu, Huan Chen, Yu Feng, Zhihui Zhou, Yunsong Yu & Xiaoting Hua
Acinetobacter baumannii is an important nosocomial pathogen. Upon colonizing a host, A. baumannii are subjected to selective pressure by immune defenses as they adapt to the host environment. However, the mechanism of this pathoadaptation is unknown. Here, we established an in vitro system to evolve A. baumannii driven by the continuous selective pressure exerted by epithelial cells, and we used a combination of experimental evolution, phenotypic characterization and multi-omics analysis to address the underlying mechanism....

Registration Year

  • 2022

Resource Types

  • Other


  • Jilin University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Fudan University
  • Sichuan University
  • University of Chinese Academy of Sciences
  • Zhejiang University
  • Capital Medical University
  • Tongji University
  • Huazhong University of Science and Technology